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PR01241

Identifier
GPROTEINAFNG  [View Relations]  [View Alignment]  
Accession
PR01241
No. of Motifs
5
Creation Date
17-DEC-1999
Title
Fungal G-protein alpha subunit signature
Database References
PRINTS; PR00318 GPROTEINA
INTERPRO; IPR002975
PDB; 1GIA
SCOP; 1GIA
Literature References
1. LINDER, M.E. AND GILMAN, A.G.
G proteins.
SCI.AM. 267 56 (1992).
 
2. KAZIRO, Y., ITOH, H., KOZASA, T., NAKAFUKU, M. AND SATOH, T.
Structure and function of signal-transducing GTP-binding proteins.
ANNU.REV.BIOCHEMISTRY 60 349-400 (1991).
 
3. SIMON, M.I., STRATHMANN, M.P. AND GAUTAM, N.
Diversity of G proteins in signal transduction.
SCIENCE 252 802-808 (1993).
 
4. BOLKER, M.
Heterotrimeric G proteins in fungal mating and pathogenesis.
FUNGAL GENETICS AND BIOLOGY 25(3) 143-156.

Documentation
Guanine nucleotide binding proteins (G proteins) are a family of membrane-
associated proteins that couple extracellularly-activated integral-membrane
receptors to intracellular effectors, such as ion channels and enzymes that
vary the concentration of second messenger molecules [1-3]. G proteins are
composed of 3 subunits (alpha, beta and gamma) which, in the resting state,
associate as a trimer at the inner face of the plasma membrane. The alpha
subunit has a molecule of guanosine diphosphate (GDP) bound to it:
stimulation of the G protein by an activated receptor leads to its exchange
for GTP (guanosine triphosphate). This results in the separation of the
alpha from the beta and gamma subunits, which always remain tightly
associated as a dimer. Both the alpha and beta-gamma subunits are then able
to interact with effectors, either individually or in a cooperative manner.
The intrinsic GTPase activity of the alpha subunit hydrolyses the bound GTP
to GDP. This returns the alpha subunit to its inactive conformation and
allows it to reassociate with the beta-gamma subunit, thus restoring the
system to its resting state.
 
G protein alpha subunits are 350-400 amino acids in length and have
molecular weights in the range 40-45 kDa. Seventeen distinct types of alpha
subunit have been identified in mammals. These fall into 4 main groups on
the basis of both sequence similarity and function: alpha-S, alpha-Q,
alpha-I and alpha-12 [3]. Many alpha subunits are substrates for
ADP-ribosylation by cholera or pertussis toxins. They are often N-terminally
acylated, usually with myristate and/or palmitoylate, and these fatty acid
modifications are probably important for membrane association and high-
affinity interactions with other proteins. The atomic structure of the 
alpha subunit of the G protein involved in mammalian vision, transducin,
has been elucidated in both GTP- and GDB-bound forms, and shows considerable
similarity in both primary and tertiary structure in the nucleotide-binding
regions to other G proteins, such as p21-ras and EF-Tu.
 
A number of fungal alpha subunits have also been identified. In
S.cerevisiae, the G protein alpha-1 subunit (GP1-alpha) is involved in
mating factor signal transduction, while the alpha-2 subunit is thought to
regulate intracellular levels of cyclic AMP [4]. Unlike in mammalian cells,
in yeast it is the beta-gamma dimer that activates the effector rather than
the alpha subunit. The sequence identity between different fungal alpha 
subunits is relatively low and is equivalent to the level of similarity
observed between mammalian alpha subtypes. The GTP-binding and hydrolysis
regions, however, show remarkable conservation.
 
GPROTEINAFNG is a 5-element fingerprint that provides a signature for the
fungal G protein alpha subunit. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections 
that characterise fungal alpha subunits but distinguish them from other
G protein alpha subtypes. Two iterations on SPTR37_10f were required to
reach convergence, at which point a true set comprising 15 sequences was 
identified. Several partial matches were also found, many of which are
fungal G protein alpha subunits that lack significant matches with one or
more motifs; GBI_HOMAM, GBI_LYMST and GB05_DROME are non-fungal alpha-I/O 
G proteins that match 2 motifs.
Summary Information
  15 codes involving  5 elements
1 codes involving 4 elements
0 codes involving 3 elements
7 codes involving 2 elements
Composite Feature Index
51515151515
411011
300000
240541
12345
True Positives
GBA1_CANAL    GBA1_COLTR    GBA1_CRYPA    GBA1_EMENI    
GBA1_MAGGR GBA1_NEUCR GBA1_SCHPO GBA1_USTMA
GBA1_YEAST GBA2_NEUCR O13316 O74227
O74259 O74768 P78705
True Positive Partials
Codes involving 4 elements
GBA1_COPCO
Codes involving 2 elements
GB05_DROME GBA1_CRYNE GBA2_KLULA GBA2_YEAST
GBI_HOMAM GBI_LYMST Q01711
Sequence Titles
GBA1_CANAL  GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA SUBUNIT - CANDIDA ALBICANS (YEAST). 
GBA1_COLTR GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA SUBUNIT - COLLETOTRICHUM TRIFOLII.
GBA1_CRYPA GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA SUBUNIT - CRYPHONECTRIA PARASITICA (CHE
GBA1_EMENI GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA SUBUNIT - EMERICELLA NIDULANS (ASPERGIL
GBA1_MAGGR GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA SUBUNIT - MAGNAPORTHE GRISEA (RICE BLAS
GBA1_NEUCR GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP1-ALPHA) - NEUROSPORA CRAS
GBA1_SCHPO GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP1-ALPHA) - SCHIZOSACCHAROM
GBA1_USTMA GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT - USTILAGO MAYDIS (SMUT FUNGU
GBA1_YEAST GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP1-ALPHA) - SACCHAROMYCES C
GBA2_NEUCR GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-2 SUBUNIT (GP2-ALPHA) - NEUROSPORA CRAS
O13316 MAGC - MAGNAPORTHE GRISEA (RICE BLAST FUNGUS).
O74227 GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA SUBUNIT - COCHLIOBOLUS HETEROSTROPHUS (DRECHSLERA MAYDIS).
O74259 G PROTEIN ALPHA SUBUNIT - SPOROTHRIX SCHENCKII.
O74768 GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT - SCHIZOSACCHAROMYCES POMBE (
P78705 G PROTEIN ALPHA SUBUNIT - NEUROSPORA CRASSA.

GBA1_COPCO GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA SUBUNIT - COPRINUS CONGREGATUS (INKY CA

GB05_DROME GUANINE NUCLEOTIDE-BINDING PROTEIN G(O), ALPHA SUBUNIT - DROSOPHILA MELANOGASTER
GBA1_CRYNE GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA SUBUNIT - CRYPTOCOCCUS NEOFORMANS (FILO
GBA2_KLULA GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-2 SUBUNIT (GP2-ALPHA) - KLUYVEROMYCES L
GBA2_YEAST GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-2 SUBUNIT (GP2-ALPHA) - SACCHAROMYCES C
GBI_HOMAM GUANINE NUCLEOTIDE-BINDING PROTEIN G(I), ALPHA SUBUNIT (ADENYLATE CYCLASE-INHIBI
GBI_LYMST GUANINE NUCLEOTIDE-BINDING PROTEIN G(I), ALPHA SUBUNIT (ADENYLATE CYCLASE-INHIBI
Q01711 GUANOSINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT - PNEUMOCYSTIS CARINII.
Scan History
SPTR37_10f 2  50   NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
GGYSRDERESFKE GBA1_CRYPA 58 58 -
GGYSRDERESFKE GBA1_EMENI 58 58 -
GSYSAEERESYKE GBA1_USTMA 59 59 -
QGFSKNEKLEWRP GBA1_NEUCR 60 60 -
GGFSHQERLQYAQ GBA1_YEAST 66 66 -
PGFSQVVRKQYRV GBA1_SCHPO 99 99 -
GGFTQQERRQYSH GBA1_CANAL 66 66 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TETTFIIGDLT GBA1_CRYPA 184 113 -
TETTFIIGDLT GBA1_EMENI 184 113 -
TETHFKIGELN GBA1_USTMA 185 113 -
TETVFDLGQLT GBA1_NEUCR 186 113 -
TETEFNIGSSK GBA1_YEAST 303 224 -
SEETFLLNRHH GBA1_SCHPO 224 112 -
TETDFLIKSFQ GBA1_CANAL 260 181 -

Motif 3 width=13
Element Seqn Id St Int Rpt
KEKLPVSPMKNYF GBA1_CRYPA 274 79 -
KEKLPVSPMKNYF GBA1_EMENI 274 79 -
KQKLPISPMADYF GBA1_USTMA 275 79 -
KEKLPRSPITNHG GBA1_NEUCR 276 79 -
EEKVKSMPIRKYF GBA1_YEAST 393 79 -
KRKGSHFPIQKHF GBA1_SCHPO 314 79 -
ENKIKKNPLKNYF GBA1_CANAL 350 79 -

Motif 4 width=12
Element Seqn Id St Int Rpt
YILNRFVSLNQH GBA1_CRYPA 301 14 -
YILNRFVSLNQA GBA1_EMENI 301 14 -
YIVNRFVSLNQS GBA1_USTMA 302 14 -
YFMDKFRALNRN GBA1_NEUCR 305 16 -
YFEKIFLSLNKT GBA1_YEAST 421 15 -
YFYLKFESLNRI GBA1_SCHPO 356 29 -
FFETNFLKINQT GBA1_CANAL 378 15 -

Motif 5 width=11
Element Seqn Id St Int Rpt
TQIRFVMAAVN GBA1_CRYPA 328 15 -
TQIRFVMAAVN GBA1_EMENI 328 15 -
SQIKFVMSAVN GBA1_USTMA 329 15 -
NLLKITMGSVQ GBA1_NEUCR 332 15 -
QTMKFVLSAVT GBA1_YEAST 447 14 -
SLLQRVMVSVQ GBA1_SCHPO 383 15 -
KSMKFVLSAVT GBA1_CANAL 404 14 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
GGYSRDERESFKE GBA1_COLTR 58 58 -
GGYSRDERESFKE GBA2_NEUCR 59 59 -
GGYSRDERESFKE GBA1_CRYPA 58 58 -
GGYSRDERESFKE GBA1_MAGGR 58 58 -
GGYSRDERESFKE GBA1_EMENI 58 58 -
GGYSRDERESFKE O74227 58 58 -
GGYSRDGAESFKE P78705 59 59 -
GGYSRDERESFKE O74259 59 59 -
GSYSAEERESYKE GBA1_USTMA 59 59 -
QGFSKNEKLEWRP GBA1_NEUCR 60 60 -
GGFSHQERLQYAQ GBA1_YEAST 66 66 -
QGFSKNEKIEWKP O13316 60 60 -
PGFSQVVRKQYRV O74768 100 100 -
PGFSQVVRKQYRV GBA1_SCHPO 99 99 -
GGFTQQERRQYSH GBA1_CANAL 66 66 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TETTFIIGDLT GBA1_COLTR 184 113 -
TETTFIIGDLT GBA2_NEUCR 185 113 -
TETTFIIGDLT GBA1_CRYPA 184 113 -
TETTFIIGDLT GBA1_MAGGR 184 113 -
TETTFIIGDLT GBA1_EMENI 184 113 -
TETTFIIGDLT O74227 184 113 -
TETTFIIGDLT P78705 185 113 -
TEKTFIIGDLT O74259 185 113 -
TETHFKIGELN GBA1_USTMA 185 113 -
TETVFDLGQLT GBA1_NEUCR 186 113 -
TETEFNIGSSK GBA1_YEAST 303 224 -
TETVFDLGQLT O13316 186 113 -
SEETFLLNRHH O74768 225 112 -
SEETFLLNRHH GBA1_SCHPO 224 112 -
TETDFLIKSFQ GBA1_CANAL 260 181 -

Motif 3 width=13
Element Seqn Id St Int Rpt
KEKLPVSPMKNYF GBA1_COLTR 274 79 -
KEKLPVSPMKNYF GBA2_NEUCR 275 79 -
KEKLPVSPMKNYF GBA1_CRYPA 274 79 -
KEKLPISPMKNYF GBA1_MAGGR 274 79 -
KEKLPVSPMKNYF GBA1_EMENI 274 79 -
KEKLPVSPMKNYF O74227 274 79 -
KEKLPVSPMKNYF P78705 275 79 -
KEKLPISPMKNYF O74259 275 79 -
KQKLPISPMADYF GBA1_USTMA 275 79 -
KEKLPRSPITNHG GBA1_NEUCR 276 79 -
EEKVKSMPIRKYF GBA1_YEAST 393 79 -
KEKITKSPITEYG O13316 276 79 -
KRKVHISPIQKHF O74768 315 79 -
KRKGSHFPIQKHF GBA1_SCHPO 314 79 -
ENKIKKNPLKNYF GBA1_CANAL 350 79 -

Motif 4 width=12
Element Seqn Id St Int Rpt
YILNRFVSLNQH GBA1_COLTR 301 14 -
YILNRFVSLNQH GBA2_NEUCR 302 14 -
YILNRFVSLNQH GBA1_CRYPA 301 14 -
YILNRFVSLNQH GBA1_MAGGR 301 14 -
YILNRFVSLNQA GBA1_EMENI 301 14 -
YILNRFVSLNQH O74227 301 14 -
YILNRFVSLNQH P78705 302 14 -
YILNRFVNLNQH O74259 302 14 -
YIVNRFVSLNQS GBA1_USTMA 302 14 -
YFMDKFRALNRN GBA1_NEUCR 305 16 -
YFEKIFLSLNKT GBA1_YEAST 421 15 -
YFMDKFRALNRN O13316 305 16 -
YFYLKFESLNRI O74768 357 29 -
YFYLKFESLNRI GBA1_SCHPO 356 29 -
FFETNFLKINQT GBA1_CANAL 378 15 -

Motif 5 width=11
Element Seqn Id St Int Rpt
TQIRFVMAAVN GBA1_COLTR 328 15 -
TQIRFVMAAVN GBA2_NEUCR 329 15 -
TQIRFVMAAVN GBA1_CRYPA 328 15 -
TQIRFVMAAVN GBA1_MAGGR 328 15 -
TQIRFVMAAVN GBA1_EMENI 328 15 -
MQIRFVMAAVN O74227 328 15 -
TQIRFVMAAVN P78705 329 15 -
TQIRFVMAAVN O74259 329 15 -
SQIKFVMSAVN GBA1_USTMA 329 15 -
NLLKITMGSVQ GBA1_NEUCR 332 15 -
QTMKFVLSAVT GBA1_YEAST 447 14 -
NLLKITMTSVQ O13316 332 15 -
SLLQRVMVSVQ O74768 384 15 -
SLLQRVMVSVQ GBA1_SCHPO 383 15 -
KSMKFVLSAVT GBA1_CANAL 404 14 -