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PR01235

Identifier
TNFALPHA  [View Relations]  [View Alignment]  
Accession
PR01235
No. of Motifs
6
Creation Date
20-OCT-1999
Title
Tumour necrosis factor alpha (cachectin) signature
Database References
PRINTS; PR01234 TNECROSISFCT
INTERPRO; IPR002959
PDB; 1TNF
SCOP; 1TNF
CATH; 1TNF
Literature References
1. FRANSEN, L., MULLER, R., MARMENOUT, A., TAVERNIER, J., VAN DER
HEYDEN, J., KAWASHIMA, E., CHOLLET, A., TIZARD, R., VAN HEUVERSWYN, H.
AND VAN VLIET, A.
Molecular cloning of mouse tumour necrosis factor cDNA and its eukaryotic
expression.
NUCLEIC ACIDS RES. 13 4417-4429 (1985).
 
2. KRIEGLER, M., PEREZ, C., DEFAY, K., ALBERT, I. AND LU, S.D. 
A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane
protein: ramifications for the complex physiology of TNF.
CELL 53 45-53 (1988). 
 
3. SHERRY, B., JUE, D.M., ZENTELLA, A. AND CERAMI, A.
Characterization of high molecular weight glycosylated forms of murine 
tumor necrosis factor.
BIOCHEM.BIOPHYS.RES.COMMUN. 173 1072-1078 (1990). 
 
4. CSEH, K. AND BEUTLER, B.
Alternative cleavage of the cachectin/tumor necrosis factor propeptide 
results in a larger, inactive form of secreted protein.
J.BIOL.CHEM. 264 16256-16260 (1989). 
 
5. JONES, E.Y., STUART, D.I. AND WALKER, N.P.
Structure of tumour necrosis factor.
NATURE 338 225-228 (1989). 
 
6. VAN OSTADE X., TAVERNIER J., PRANGE T. AND FIERS W.
Localization of the active site of human tumour necrosis factor (hTNF) by
mutational analysis.
EMBO J. 10 827-836 (1991).

Documentation
Tumor necrosis factor (TNF) (or cachectin) is a monocyte-derived cytotoxin
that has been implicated in tumour regression, septic shock and cachexia
[1,2]. The protein is synthesised as a prohormone with an unusually long 
and atypical signal sequence, which is absent from the mature secreted
cytokine [3]. A short hydrophobic stretch of amino acids serves to anchor 
the prohormone in lipid bilayers [4]. Both the mature protein and a 
partially-processed form of the hormone are secreted after cleavage of the
propeptide [4]. 
 
The structure of TNF has been determined to 2.9A using X-ray crystallography
[5]. The protein is trimeric, each subunit consisting of an anti-parallel
beta-sandwich. The subunits trimerise via a novel edge-to-face packing of
beta-sheets [5]. Comparison of the subunit fold with that of other proteins
reveals similarity to the `jelly-roll' structural motif characteristic of
viral coat proteins [5]. It is believed that each TNF molecule has three 
receptor-interaction sites (between the three subunits), thus allowing
signal transmission by receptor clustering [6]. 
 
TNF-alpha is a soluble cytokine with a wide variety of functions: it causes
damage to tumour cells but has no effect on normal cells; it is involved in 
the induction of cachexia; it is a potent pyrogen, causing fever by direct
action or by stimulation of interleukin-1 secretion; and it can stimulate
cell proliferation and induce cell differentiation under certain conditions.
 
TNFALPHA is a 6-element fingerprint that provides a signature for tumour 
necrosis factor alpha. The fingerprint was derived from an initial alignment
of 13 sequences: the motifs were drawn from conserved regions spanning 
virtually the full alignment length, focusing on those sections that 
characterise TNF-alpha but distinguish it from the rest of the TNF family -
motifs 1-3 were drawn from the N-terminal region; motif 4 spans beta-strands
2 and 3; motif 5 encodes the N-terminus of strnd 9; and motif 6 spans strand
10 and helix 1. Two iterations on SPTR37_10f were required to reach
convergence, at which point a true set comprising 22 sequences was 
identified.
Summary Information
22 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6222222222222
5000000
4000000
3000000
2000000
123456
True Positives
O18779        O35853        O77764        Q28320        
TNFA_BOVIN TNFA_CANFA TNFA_CAVPO TNFA_FELCA
TNFA_HORSE TNFA_HUMAN TNFA_MACFA TNFA_MACMU
TNFA_MARMO TNFA_MOUSE TNFA_PAPHU TNFA_PAPSP
TNFA_PERLE TNFA_PIG TNFA_RABIT TNFA_RAT
TNFA_SHEEP TNFA_TRIVU
Sequence Titles
O18779      TUMOR NECROSIS FACTOR ALPHA - BOS TAURUS (BOVINE). 
O35853 TUMOR NECROSIS FACTOR ALPHA - MUS MUSCULUS (MOUSE).
O77764 TUMOUR NECROSIS FACTOR ALPHA - MACROPUS EUGENII (TAMMAR WALLABY).
Q28320 TNF-ALPHA - CAPRA HIRCUS (GOAT).
TNFA_BOVIN TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - BOS TAURUS (BOVINE).
TNFA_CANFA TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - CANIS FAMILIARIS (DOG)
TNFA_CAVPO TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - CAVIA PORCELLUS (GUINE
TNFA_FELCA TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - FELIS SILVESTRIS CATUS
TNFA_HORSE TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - EQUUS CABALLUS (HORSE)
TNFA_HUMAN TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - HOMO SAPIENS (HUMAN).
TNFA_MACFA TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - MACACA FASCICULARIS (C
TNFA_MACMU TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - MACACA MULATTA (RHESUS
TNFA_MARMO TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - MARMOTA MONAX (WOODCHU
TNFA_MOUSE TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - MUS MUSCULUS (MOUSE).
TNFA_PAPHU TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - PAPIO HAMADRYAS URSINU
TNFA_PAPSP TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - PAPIO SP. (BABOON).
TNFA_PERLE TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - PEROMYSCUS LEUCOPUS (W
TNFA_PIG TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - SUS SCROFA (PIG).
TNFA_RABIT TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - ORYCTOLAGUS CUNICULUS
TNFA_RAT TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - RATTUS NORVEGICUS (RAT
TNFA_SHEEP TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - OVIS ARIES (SHEEP).
TNFA_TRIVU TUMOR NECROSIS FACTOR PRECURSOR (TNF-ALPHA) (CACHECTIN) - TRICHOSURUS VULPECULA
Scan History
SPTR37_10f 2  40   NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
STESMIRDVELAEEALPKK TNFA_PAPSP 2 2 -
STESMIRDVELAEEALPKK TNFA_HUMAN 2 2 -
STESMIRDVELAEEALAKK TNFA_PIG 2 2 -
STESMIRDVELAEEALPRK TNFA_MACMU 2 2 -
STESMIRDVELAEEELAKK TNFA_HORSE 2 2 -
STESMIQDVELAEEALPRK TNFA_MACFA 2 2 -
STESMIRDVELAEEALPKK TNFA_PERLE 2 2 -
STKSMIRDVELAEEVLSEK TNFA_BOVIN 2 2 -
STKSMIRDVELAEEVLSNK TNFA_SHEEP 2 2 -
STESMIRDVELAEEALPQK TNFA_MOUSE 2 2 -
STESMIRDVELAEGPLPKK TNFA_RABIT 2 2 -
STESMIRDVELAEEALPKK TNFA_RAT 2 2 -
STESMIRDLELAEEALKRK TNFA_TRIVU 2 2 -

Motif 2 width=16
Element Seqn Id St Int Rpt
KTGGPQGSRRCLFLSL TNFA_PAPSP 20 -1 -
KTGGPQGSRRCLFLSL TNFA_HUMAN 20 -1 -
KAGGPQGSRRCLCLSL TNFA_PIG 20 -1 -
KTAGPQGSRRCWFLSL TNFA_MACMU 20 -1 -
KAGGPQGSRRCLCLSL TNFA_HORSE 20 -1 -
KTAGPQGSRRCWFLSL TNFA_MACFA 20 -1 -
KAWGPQNSSRCLCLSL TNFA_PERLE 20 -1 -
KAGGPQGSRSCLCLSL TNFA_BOVIN 20 -1 -
KAGGPQGSRSCWCLSL TNFA_SHEEP 20 -1 -
KMGGFQNSRRCLCLSL TNFA_MOUSE 20 -1 -
KAGGPQGSKRCLCLSL TNFA_RABIT 20 -1 -
KMGGLQNSRRCLCLSL TNFA_RAT 20 -1 -
KARGPLGLGRCLCLTL TNFA_TRIVU 20 -1 -

Motif 3 width=20
Element Seqn Id St Int Rpt
GATTLFCLLHFGVIGPQREE TNFA_PAPSP 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_HUMAN 43 7 -
GATTLFCLLHFEVIGPQKEE TNFA_PIG 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_MACMU 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_HORSE 43 7 -
GAATLFCLLHFGVIGPQREE TNFA_MACFA 43 7 -
GATTLFCLLNFGVIGPQREE TNFA_PERLE 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_BOVIN 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_SHEEP 43 7 -
GATTLFCLLNFGVIGPQRDE TNFA_MOUSE 43 7 -
GATTLFCLLHFRVIGPQEEE TNFA_RABIT 43 7 -
GATTLFCLLNFGVIGPNKEE TNFA_RAT 43 7 -
GATVLFCLLHFGVIGPQKEQ TNFA_TRIVU 43 7 -

Motif 4 width=15
Element Seqn Id St Int Rpt
ANALLANGVELRDNQ TNFA_PAPSP 109 46 -
ANALLANGVELRDNQ TNFA_HUMAN 109 46 -
ANALLANGVKLKDNQ TNFA_PIG 108 45 -
ANALLANGVELTDNQ TNFA_MACMU 109 46 -
ANALLANGVKLTDNQ TNFA_HORSE 110 47 -
ANALVANGVELTDNQ TNFA_MACFA 109 46 -
ANALLANGMDLKDNQ TNFA_PERLE 112 49 -
ANALMANGVKLEDNQ TNFA_BOVIN 109 46 -
ANALMANGVELKDNQ TNFA_SHEEP 110 47 -
ANALLANGMDLKDNQ TNFA_MOUSE 112 49 -
ANALLANGMKLTDNQ TNFA_RABIT 112 49 -
ANALLANGMDLKDNQ TNFA_RAT 112 49 -
ANTLLMNGMELVDNQ TNFA_TRIVU 109 46 -

Motif 5 width=22
Element Seqn Id St Int Rpt
KSPCQRETPEGAEAKPWYEPIY TNFA_PAPSP 174 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_HUMAN 174 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_PIG 173 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_MACMU 174 50 -
KSPCHTESPEQAEAKPWYEPIY TNFA_HORSE 175 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_MACFA 174 50 -
KSPCPKETPEGSELKPWYEPIY TNFA_PERLE 176 49 -
KSPCHRETPEWAEAKPWYEPIY TNFA_BOVIN 174 50 -
KSPCHRETLEGAEAKPWYEPIY TNFA_SHEEP 175 50 -
KSPCPKDTPEGAELKPWYEPIY TNFA_MOUSE 176 49 -
KSPCHRETPEEAEPMAWYEPIY TNFA_RABIT 176 49 -
KSPCPKDTPEGAELKPWYEPMY TNFA_RAT 176 49 -
RSSCRKAAEDDGEPSAWYEPVY TNFA_TRIVU 174 50 -

Motif 6 width=20
Element Seqn Id St Int Rpt
DRLSAEINLPDYLDFAESGQ TNFA_PAPSP 206 10 -
DRLSAEINRPDYLDFAESGQ TNFA_HUMAN 206 10 -
DRLSAEINLPDYLDFAESGQ TNFA_PIG 205 10 -
DRLSAEINLPDYLDFAESGQ TNFA_MACMU 206 10 -
DQLSAEINQPNYLDFAESGQ TNFA_HORSE 207 10 -
DRLSAEINLPDYLDFAESGQ TNFA_MACFA 206 10 -
DRLSAEVNLPKYLDFAESGQ TNFA_PERLE 208 10 -
DRLSAEINLPDYLDYAESGQ TNFA_BOVIN 206 10 -
DRLSAEINLPEYLDYAESGQ TNFA_SHEEP 207 10 -
DQLSAEVNLPKYLDFAESGQ TNFA_MOUSE 208 10 -
DRLSTEVNQPEYLDLAESGQ TNFA_RABIT 208 10 -
DLLSAEVNLPKYLDITESGQ TNFA_RAT 208 10 -
DKLVVDTNYPENLDFAEPGQ TNFA_TRIVU 206 10 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
STESMIRDVELAEEALPKK TNFA_PAPSP 2 2 -
STESMIRDVELAEEALPKK TNFA_FELCA 2 2 -
STESMIRDVELAEEALPKK TNFA_HUMAN 2 2 -
STESMIRDVELAEEALAKK TNFA_PIG 2 2 -
STESMIRDVELAEEALPRK TNFA_PAPHU 2 2 -
STESMIRDVELAEEALPRK TNFA_MACMU 2 2 -
STESMIRDVELAEEPLPKK TNFA_CANFA 2 2 -
STESMIRDVELAEEQLPKK TNFA_CAVPO 2 2 -
STESMIRDVELAEEELAKK TNFA_HORSE 2 2 -
STKSMIRDVELAEEVLSKK Q28320 2 2 -
STESMIQDVELAEEALPRK TNFA_MACFA 2 2 -
STESMIRDVELAEEALPKK TNFA_PERLE 2 2 -
STKSMIRDVELAEEVLSEK TNFA_BOVIN 2 2 -
STKSMIRDVELAEEVLSNK TNFA_SHEEP 2 2 -
STKSMIRDVELAEEVLSEK O18779 2 2 -
STESMIRDVELAEEALPKE TNFA_MARMO 2 2 -
STESMIRDVELAEEALPQK TNFA_MOUSE 2 2 -
STESMTRDVELAEEALPQK O35853 2 2 -
STESMIRDVELAEGPLPKK TNFA_RABIT 2 2 -
STESMIRDVELAEEALPKK TNFA_RAT 2 2 -
STENMIRDVELAEEELQRK O77764 2 2 -
STESMIRDLELAEEALKRK TNFA_TRIVU 2 2 -

Motif 2 width=16
Element Seqn Id St Int Rpt
KTGGPQGSRRCLFLSL TNFA_PAPSP 20 -1 -
KAGGPQGSGRCLCLSL TNFA_FELCA 20 -1 -
KTGGPQGSRRCLFLSL TNFA_HUMAN 20 -1 -
KAGGPQGSRRCLCLSL TNFA_PIG 20 -1 -
KTAGPQGSRRCWFLSL TNFA_PAPHU 20 -1 -
KTAGPQGSRRCWFLSL TNFA_MACMU 20 -1 -
KAGGPPGSRRCFCLSL TNFA_CANFA 20 -1 -
KAGGPQGSRRCWCLSL TNFA_CAVPO 20 -1 -
KAGGPQGSRRCLCLSL TNFA_HORSE 20 -1 -
KAGGPQGSRSCWCLSL Q28320 20 -1 -
KTAGPQGSRRCWFLSL TNFA_MACFA 20 -1 -
KAWGPQNSSRCLCLSL TNFA_PERLE 20 -1 -
KAGGPQGSRSCLCLSL TNFA_BOVIN 20 -1 -
KAGGPQGSRSCWCLSL TNFA_SHEEP 20 -1 -
KAGGPQGSRSCLCLSL O18779 20 -1 -
EAWGPQGSSRCLCLSL TNFA_MARMO 20 -1 -
KMGGFQNSRRCLCLSL TNFA_MOUSE 20 -1 -
KMGGFQNSRRCLCLSL O35853 20 -1 -
KAGGPQGSKRCLCLSL TNFA_RABIT 20 -1 -
KMGGLQNSRRCLCLSL TNFA_RAT 20 -1 -
KARGPQGPGRCLCLIL O77764 20 -1 -
KARGPLGLGRCLCLTL TNFA_TRIVU 20 -1 -

Motif 3 width=20
Element Seqn Id St Int Rpt
GATTLFCLLHFGVIGPQREE TNFA_PAPSP 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_FELCA 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_HUMAN 43 7 -
GATTLFCLLHFEVIGPQKEE TNFA_PIG 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_PAPHU 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_MACMU 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_CANFA 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_CAVPO 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_HORSE 43 7 -
GATTLFCLLHFGVIGPQREE Q28320 43 7 -
GAATLFCLLHFGVIGPQREE TNFA_MACFA 43 7 -
GATTLFCLLNFGVIGPQREE TNFA_PERLE 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_BOVIN 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_SHEEP 43 7 -
GATTLCCLLHFGVIGPQREE O18779 43 7 -
GATTLFCLLHFGVIGPQREE TNFA_MARMO 43 7 -
GATTLFCLLNFGVIGPQRDE TNFA_MOUSE 43 7 -
GATTLFCLLNFGVIGPQRDE O35853 43 7 -
GATTLFCLLHFRVIGPQEEE TNFA_RABIT 43 7 -
GATTLFCLLNFGVIGPNKEE TNFA_RAT 43 7 -
GATLLFCLLHFGVIGPQNEE O77764 43 7 -
GATVLFCLLHFGVIGPQKEQ TNFA_TRIVU 43 7 -

Motif 4 width=15
Element Seqn Id St Int Rpt
ANALLANGVELRDNQ TNFA_PAPSP 109 46 -
ANALLANGVELTDNQ TNFA_FELCA 109 46 -
ANALLANGVELRDNQ TNFA_HUMAN 109 46 -
ANALLANGVKLKDNQ TNFA_PIG 108 45 -
ANALLANGVELTDNQ TNFA_PAPHU 109 46 -
ANALLANGVELTDNQ TNFA_MACMU 109 46 -
ANALLANGVELTDNQ TNFA_CANFA 109 46 -
ANALLANGMGLSDNQ TNFA_CAVPO 111 48 -
ANALLANGVKLTDNQ TNFA_HORSE 110 47 -
ANALKANGVELKDNQ Q28320 110 47 -
ANALVANGVELTDNQ TNFA_MACFA 109 46 -
ANALLANGMDLKDNQ TNFA_PERLE 112 49 -
ANALMANGVKLEDNQ TNFA_BOVIN 109 46 -
ANALMANGVELKDNQ TNFA_SHEEP 110 47 -
ANALMANGVKLEDNQ O18779 109 46 -
ANALLANGMELIDNQ TNFA_MARMO 110 47 -
ANALLANGMDLKDNQ TNFA_MOUSE 112 49 -
ANALLANGMDLKDNQ O35853 109 46 -
ANALLANGMKLTDNQ TNFA_RABIT 112 49 -
ANALLANGMDLKDNQ TNFA_RAT 112 49 -
ANVLLSNGMDLVDNQ O77764 109 46 -
ANTLLMNGMELVDNQ TNFA_TRIVU 109 46 -

Motif 5 width=22
Element Seqn Id St Int Rpt
KSPCQRETPEGAEAKPWYEPIY TNFA_PAPSP 174 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_FELCA 174 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_HUMAN 174 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_PIG 173 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_PAPHU 174 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_MACMU 174 50 -
KSPCQRETPEGTEAKPWYEPIY TNFA_CANFA 174 50 -
KSPCQKETPEGAERKPWYEPIY TNFA_CAVPO 175 49 -
KSPCHTESPEQAEAKPWYEPIY TNFA_HORSE 175 50 -
KSPCHRETPEGAEAKPWYEPIY Q28320 175 50 -
KSPCQRETPEGAEAKPWYEPIY TNFA_MACFA 174 50 -
KSPCPKETPEGSELKPWYEPIY TNFA_PERLE 176 49 -
KSPCHRETPEWAEAKPWYEPIY TNFA_BOVIN 174 50 -
KSPCHRETLEGAEAKPWYEPIY TNFA_SHEEP 175 50 -
KSPCHRETPEWAEAKPWYEPIY O18779 174 50 -
KSPCPKESLEGAEFKPWYEPIY TNFA_MARMO 174 49 -
KSPCPKDTPEGAELKPWYEPIY TNFA_MOUSE 176 49 -
KSPCPKDTPEGAELKPWYEPIY O35853 173 49 -
KSPCHRETPEEAEPMAWYEPIY TNFA_RABIT 176 49 -
KSPCPKDTPEGAELKPWYEPMY TNFA_RAT 176 49 -
KSPCQKTVKGAREASPWYEPIY O77764 174 50 -
RSSCRKAAEDDGEPSAWYEPVY TNFA_TRIVU 174 50 -

Motif 6 width=20
Element Seqn Id St Int Rpt
DRLSAEINLPDYLDFAESGQ TNFA_PAPSP 206 10 -
DRLSTEINLPAYLDFAESGQ TNFA_FELCA 206 10 -
DRLSAEINRPDYLDFAESGQ TNFA_HUMAN 206 10 -
DRLSAEINLPDYLDFAESGQ TNFA_PIG 205 10 -
DRLSAEINLPDYLDFAESGQ TNFA_PAPHU 206 10 -
DRLSAEINLPDYLDFAESGQ TNFA_MACMU 206 10 -
DRLSAEINLPNYLDFAESGQ TNFA_CANFA 206 10 -
DRLSAEVNLPQYLDFADSGQ TNFA_CAVPO 207 10 -
DQLSAEINQPNYLDFAESGQ TNFA_HORSE 207 10 -
DRLSAEINQPEYLDYAESGQ Q28320 207 10 -
DRLSAEINLPDYLDFAESGQ TNFA_MACFA 206 10 -
DRLSAEVNLPKYLDFAESGQ TNFA_PERLE 208 10 -
DRLSAEINLPDYLDYAESGQ TNFA_BOVIN 206 10 -
DRLSAEINLPEYLDYAESGQ TNFA_SHEEP 207 10 -
DRLSAEINLPDYLDYAESGQ O18779 206 10 -
DRLSAEVNLPSYLDFAESGQ TNFA_MARMO 206 10 -
DQLSAEVNLPKYLDFAESGQ TNFA_MOUSE 208 10 -
DQLSAEVNLPKYLDFAESGQ O35853 205 10 -
DRLSTEVNQPEYLDLAESGQ TNFA_RABIT 208 10 -
DLLSAEVNLPKYLDITESGQ TNFA_RAT 208 10 -
DKLSADTNYPNYLDFAESGQ O77764 206 10 -
DKLVVDTNYPENLDFAEPGQ TNFA_TRIVU 206 10 -