| Literature References | 1. NILSSON, O., LUNDQVIST, T., HAHNE, S. AND SJOBERG, B.-M.
Structure-function studies of the large subunit of ribonucleotide reductase
from Escherichia coli.
BIOCHEM.SOC.TRANS. 16 91-94 (1988).
2. REICHARD, P.
From RNA to DNA, why so many ribonucleotide reductases?
SCIENCE 260 1773-1777 (1993).
3. ERIKSSON, M., UHLIN, U., S.R., EKBERG, M., REGNSTROM, K., SJOBERG, B.M.
AND EKLUND, H.
Binding of allosteric effectors to ribonucleotide reductase protein R1:
reduction of active-site cysteines promotes substrate binding.
STRUCTURE 5 1077-1092 (1997).
4. UHLIN, U. AND EKLUND, H.
Structure of ribonucleotide reductase protein R1.
NATURE 370 533-539 (1994).
5. CARAS, I.W., LEVINSON, B.B., FABRY, M., WILLIAMS, S.R. AND MARTIN, D.W.JR.
Cloned mouse ribonucleotide reductase subunit M1 cDNA reveals amino acid
sequence homology with Escherichia coli and herpesvirus ribonucleotide
reductases.
J.BIOL.CHEM. 260 7015-7022 (1985).
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| Documentation | Ribonucleotide reductase catalyses the reductive synthesis of deoxy-
ribonucleotides from their corresponding ribonucleotides, providing the
necessary precursors for DNA synthesis [1,2]. The enzyme comprises two
dimers (termed R1 and R2), and contains several redox active cysteine
residues. The reduction of ribonucleotides to deoxyribonucleotides involves
the transfer of free radicals [3]. The radical-based reaction involves five
cysteines: two of these are located at adjacent anti-parallel strands in a
new type of ten-stranded alpha/beta-barrel; two others reside at the
carboxyl end in a flexible arm; and the fifth, in a loop in the centre of
the barrel, is positioned to initiate the radical reaction [4].
There are several regions of similarity in the sequence of the large
chain of prokaryotes, eukaryotes and viruses spread across 3 domains:
an N-terminal domain common to the mammalian and bacterial enzymes; a
C-terminal domain common to the mammalian and viral ribonucleotide
reductases; and a central domain common to all three [3].
RIBORDTASEM1 is a 6-element fingerprint that provides a signature for
ribonucleotide reductase M1 (large) chains. The fingerprint was derived
from an initial alignment of 11 sequences: the motifs were drawn from short
conserved regions spanning the C-terminal half of the alignment. Five
iterations on SPTR37_10f were required to reach convergence, at which point
a true set comprising 63 sequences was identified. Several partial matches
were also found, all of which are ribonucleotide reductases and homologues
that fail to match one or more motifs.
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| Sequence Titles | O12935 RIBONUCLEOTIDE REDUCTASE - SPODOPTERA EXIGUA NUCLEOPOLYHEDROVIRUS.
O28609 RIBONUCLEOTIDE REDUCTASE (NRD) - ARCHAEOGLOBUS FULGIDUS.
O33839 RIBONUCLEOTIDE REDUCTASE - THERMOTOGA MARITIMA.
O36411 RIBONUCLEOTIDE-REDUCTASE, LARGE SUBUNIT - ALCELAPHINE HERPESVIRUS 1 (WILDEBEEST
O39263 COUNTERPART OF HSV-1 GENE UL39 AND VZV GENE 19 - EQUINE HERPESVIRUS 4.
O41111 A629R PROTEIN - PARAMECIUM BURSARIA CHLORELLA VIRUS 1 (PBCV-1).
O41269 RIBONUCLEOTIDE REDUCTASE - SPODOPTERA LITTORALIS NUCLEAR POLYHEDROSIS VIRUS (SLN
O41962 RIBONUCLEOTIDE REDUCTASE LARGE - MURINE HERPESVIRUS 68.
O53767 HYPOTHETICAL 74.5 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O54196 VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE - STREPTOMYCES CLAVULIGERUS.
O66503 RIBONUCLEOTIDE REDUCTASE ALPHA CHAIN - AQUIFEX AEOLICUS.
O69273 RIBONUCLEOTIDE REDUCTASE SUBUNIT R1E - CORYNEBACTERIUM AMMONIAGENES (BREVIBACTER
O69981 RIBONUCLEOTIDE REDUCTASE - STREPTOMYCES COELICOLOR.
O72889 RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT (EC 1.17.4.1) - FELINE HERPESVIRUS (FELID
O82573 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT - ARABIDOPSIS THALIANA (MOUSE
O84834 RIBONUCLEOSIDE REDUCTASE, LARGE CHAIN - CHLAMYDIA TRACHOMATIS.
P74911 RIBONUCLEOTIDE REDUCTASE - THERMOPLASMA ACIDOPHILUM.
P88952 RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT (ORF 61) - KAPOSI'S SARCOMA-ASSOCIATED HE
P89462 RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT - HERPES SIMPLEX VIRUS (TYPE 2).
Q47412 RIBONUCLEOSIDE DIPHOSPHATE REDUCTASE B1 SUBUNIT ALPHA-PRIME-POLYPEPTIDE PRECURSO
Q66663 RIBONUCLEOTIDE REDUCTASE, LARGE SUBUNIT - EQUINE HERPESVIRUS 2.
Q9YTK6 LARGE SUBUNIT OF RIBONUCLEOTIDE REDUCTASE - ATELINE HERPESVIRUS 3.
Q9Z6S5 RIBONUCLEOSIDE REDUCTASE, LARGE CHAIN - CHLAMYDIA PNEUMONIAE.
Q9ZLF9 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 ALPHA CHAIN - HELICOBACTER PYLORI J99.
Q9ZR56 RIBONUCLEOTIDE REDUCTASE - NICOTIANA TABACUM (COMMON TOBACCO).
Q9ZR57 RIBONUCLEOTIDE REDUCTASE - NICOTIANA TABACUM (COMMON TOBACCO).
RIR1_ASFB7 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_ASFM2 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_BPT4 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_BRARE RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE M1 CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE REDU
RIR1_CAEEL PROBABLE RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUC
RIR1_CRYPV RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_EBV RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_ECOLI RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE
RIR1_HAEIN RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HELPY RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HSV11 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HSV23 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HSVBC RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HSVE4 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HSVEB RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HSVSA RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HUMAN RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE M1 CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE REDU
RIR1_MOUSE RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE M1 CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE REDU
RIR1_MYCGE RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_MYCPN RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_MYCTU RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_PLAF4 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_PLAFG RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_PRVKA RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_SALTY RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE
RIR1_SCHPO RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_SYNY3 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_TREPA RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_TRYBB RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_VACCC RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_VACCV RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_VARV RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_VZVD RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_YEAST RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN 1 (EC 1.17.4.1) (RIBONUCLEOTIDE
RIR3_ECOLI RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 2 ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE
RIR3_SALTY RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 2 ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE
RIR3_YEAST RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN 2 (EC 1.17.4.1) (RIBONUCLEOTIDE
O26748 RIBONUCLEOTIDE REDUCTASE, LARGE SUBUNIT - METHANOBACTERIUM THERMOAUTOTROPHICUM.
Q89942 RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT - TURKEY HERPESVIRUS.
Q9Z5C8 RIBONUCELOTIDE REDUCTASE MAJOR SUBUNIT - STAPHYLOCOCCUS AUREUS.
Q9ZD34 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (NRDA) - RICKETTSIA PROWAZEKII
O58101 1291AA LONG HYPOTHETICAL RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE - PYROCOCCUS HORIK
P95484 RIBONUCLEOTIDE REDUCTASE - PYROCOCCUS FURIOSUS.
RIR1_BACSU RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
O55716 PUTATIVE RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN - CHILO IRIDESCENT VIR
O64173 RIBUNUCLEOTIDE REDUCTASE LARGE SUBUNIT - BACTERIOPHAGE SPBC2.
RIR1_HCMVA RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
O10288 RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT - ORGYIA PSEUDOTSUGATA NUCLEAR POLYHEDROS
O31874 YOSO PROTEIN (RIBONUCLEOTIDE REDUCTASE HOMOLOG) - BACILLUS SUBTILIS.
Q89941 RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT - HUMAN HERPESVIRUS 6.
Q9YMI0 RIBONUCLEOTIDE REDUCTASE SMALL SUBUNIT HOMOLOG - LYMANTRIA DISPAR MULTICAPSID NU
RIR1_HSV6U RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
RIR1_HSV7J RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN (EC 1.17.4.1) (RIBONUCLEOTIDE R
VG50_BPMD2 PUTATIVE ADENOSYLCOBALAMIN-DEPENDENT RIBONUCLEOTIDE REDUCTASE (EC 1.17.4.2) (GP5
VG50_BPML5 PUTATIVE ADENOSYLCOBALAMIN-DEPENDENT RIBONUCLEOTIDE REDUCTASE (EC 1.17.4.2) (GP5
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