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PR01178

Identifier
GABAB2RECPTR  [View Relations]  [View Alignment]  
Accession
PR01178
No. of Motifs
13
Creation Date
18-SEP-1999
Title
Metabotropic gamma-aminobutyric acid type B2 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00248 GPCRMGR
PRINTS; PR01176 GABABRECEPTR
INTERPRO; IPR002457
Literature References
1. KAUPMANN, K., HUGGEL, K., HEID, J., FLOR, P.J., BISCHOFF, S., MICKEL, S.J.,
MCMASTER, G., ANGST, C., BITTIGER, H., FROESTL, W. AND BETTLER, B.
Expression cloning of GABA(B) receptors uncovers similarity to metabotropic
glutamate receptors.
NATURE 386 239-246 (1997). 
 
2. KAUPMANN, K., SCHULER, V., MOSBACHER., J, BISCHOFF, S., BITTIGER, H.,
HEID, J., FROESTL, W., LEONHARD, S., PFAFF, T., KARSCHIN, A. AND BETTLER, B.
Human gamma-aminobutyric acid type B receptors are differentially expressed
and regulate inwardly rectifying K+ channels.
PROC.NATL.ACAD.SCI.U.S.A. 95(25) 14991-14996 (1998),
 
3. WHITE, J.H., WISE, A., MAIN, M.J., GREEN, A., FRASER, N.J., DISNEY, G.H.,
BARNES, A.A., EMSON, P., FOORD, S.M. AND MARSHALL, F.H.
Heterodimerization is required for the formation of a functional GABA(B)
receptor.
NATURE 396 679-82 (1998).

Documentation
GABA (gamma-amino-butyric acid) is the principal inhibitory neurotransmitter
in the brain, and signals through ionotropic (GABA(A)/GABA(C)) and
metabotropic (GABA(B)) receptor systems [1]. The GABA(B) receptors have 
been cloned, and photoaffinity labelling experiments suggest that they
correspond to two highly conserved receptor forms in the vertebrate nervous
system [1]. 
 
GABA(B) receptors are involved in the fine tuning of inhibitory synaptic
transmission [2]. Presynaptic receptors inhibit neurotransmitter release by
down-regulating high-voltage activated Ca2+ channels, while postsynaptic 
receptors decrease neuronal excitability by activating a prominent inwardly
rectifying K+ (Kir) conductance that underlies the late inhibitory post-
synaptic potentials [2]. GABA(B) receptors negatively couple to adenylyl
cyclase and show sequence similarity to the metabotropic receptors for the
excitatory neurotransmitter L-glutamate. 
 
A new subtype of the GABA(B) receptor (GABA(B)R2) has been identified by
EST database mining [3]. Yeast two-hybrid screening has shown that the new
subtype forms heterodimers with GABA(B)R1 via an interaction at their
intracellular C-terminal tails [3]. On expression with GABA(B)R2 in HEK293T
cells, GABA(B)R1 is terminally glycosylated and expressed at the cell 
surface. Co-expression of the receptors produces a fully functional GABA(B)
receptor at the cell surface; this receptor binds GABA with a high affinity
equivalent to that of the endogenous brain receptor [3]. Such results
indicate that, in vivo, functional brain GABA(B) receptors may be hetero-
dimers of GABA(B)R1 and GABA(B)R2.
 
GABAB2RECPTR is a 13-element fingerprint that provides a signature for 
type 2 GABA(B) receptors. The fingerprint was derived from an initial 
alignment of 2 sequences: the motifs were drawn from conserved regions 
spanning virtually the full alignment length, focusing on those sections 
that characterise the type 2 receptors but distinguish them from the rest
of the GABA(B) receptor family. A single iteration on SPTR37_10f was 
required to reach convergence, no further sequences being identified
beyond the starting set. 
Summary Information
2 codes involving 13 elements
0 codes involving 12 elements
0 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
132222222222222
120000000000000
110000000000000
100000000000000
90000000000000
80000000000000
70000000000000
60000000000000
50000000000000
40000000000000
30000000000000
20000000000000
12345678910111213
True Positives
O75899        O88871        
Sequence Titles
O75899      GABA-B RECEPTOR - HOMO SAPIENS (HUMAN).       
O88871 GABA-B RECEPTOR GB2 - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  40   NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
LAPGAWGWARGAPRPPPSS O75899 35 35 -
LAPGAWGWTRGAPRPPPSS O88871 34 34 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PLTKEVAKGSIGRGVLP O75899 63 9 -
PLTKEVAKGSIGRGVLP O88871 62 9 -

Motif 3 width=18
Element Seqn Id St Int Rpt
QIRNESLLRPYFLDLRLY O75899 87 7 -
QIRNESLLRPYFLDLRLY O88871 86 7 -

Motif 4 width=21
Element Seqn Id St Int Rpt
WEQVHTEANSSRCLRKNLLAA O75899 290 185 -
WEQVHVEANSSRCLRRSLLAA O88871 289 185 -

Motif 5 width=20
Element Seqn Id St Int Rpt
YIGVDFEPLSSKQIKTISGK O75899 314 3 -
YIGVDFEPLSSKQIKTISGK O88871 313 3 -

Motif 6 width=23
Element Seqn Id St Int Rpt
PQQYEREYNNKRSGVGPSKFHGY O75899 335 1 -
PQQFEREYNSKRSGVGPSKFHGY O88871 334 1 -

Motif 7 width=20
Element Seqn Id St Int Rpt
KTLQRAMETLHASSRHQRIQ O75899 367 9 -
KTLQRAMETLHASSRHQRIQ O88871 366 9 -

Motif 8 width=20
Element Seqn Id St Int Rpt
FRNGERMGTIKFTQFQDSRE O75899 417 30 -
FRNGERMGTIKFTQFQDSRE O88871 416 30 -

Motif 9 width=21
Element Seqn Id St Int Rpt
YNAVADTLEIINDTIRFQGSE O75899 442 5 -
YNAVADTLEIINDTIRFQGSE O88871 441 5 -

Motif 10 width=20
Element Seqn Id St Int Rpt
PDAATQNRRFQFTQNQKKED O75899 751 288 -
PDAATQNRRFQFTQNQKKED O88871 750 288 -

Motif 11 width=25
Element Seqn Id St Int Rpt
RLEGLQSENHRLRMKITELDKDLEE O75899 787 16 -
RLEGLQSENHRLRMKITELDKDLEE O88871 786 16 -

Motif 12 width=21
Element Seqn Id St Int Rpt
KAILKNHLDQNPQLQWNTTEP O75899 851 39 -
KAILKNHLDQNPQLQWNTTEP O88871 850 39 -

Motif 13 width=21
Element Seqn Id St Int Rpt
DASCVSPCVSPTASPRHRHVP O75899 911 39 -
DASCVSPCVSPTASPRHRHVP O88871 910 39 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
LAPGAWGWARGAPRPPPSS O75899 35 35 -
LAPGAWGWTRGAPRPPPSS O88871 34 34 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PLTKEVAKGSIGRGVLP O75899 63 9 -
PLTKEVAKGSIGRGVLP O88871 62 9 -

Motif 3 width=18
Element Seqn Id St Int Rpt
QIRNESLLRPYFLDLRLY O75899 87 7 -
QIRNESLLRPYFLDLRLY O88871 86 7 -

Motif 4 width=21
Element Seqn Id St Int Rpt
WEQVHTEANSSRCLRKNLLAA O75899 290 185 -
WEQVHVEANSSRCLRRSLLAA O88871 289 185 -

Motif 5 width=20
Element Seqn Id St Int Rpt
YIGVDFEPLSSKQIKTISGK O75899 314 3 -
YIGVDFEPLSSKQIKTISGK O88871 313 3 -

Motif 6 width=23
Element Seqn Id St Int Rpt
PQQYEREYNNKRSGVGPSKFHGY O75899 335 1 -
PQQFEREYNSKRSGVGPSKFHGY O88871 334 1 -

Motif 7 width=20
Element Seqn Id St Int Rpt
KTLQRAMETLHASSRHQRIQ O75899 367 9 -
KTLQRAMETLHASSRHQRIQ O88871 366 9 -

Motif 8 width=20
Element Seqn Id St Int Rpt
FRNGERMGTIKFTQFQDSRE O75899 417 30 -
FRNGERMGTIKFTQFQDSRE O88871 416 30 -

Motif 9 width=21
Element Seqn Id St Int Rpt
YNAVADTLEIINDTIRFQGSE O75899 442 5 -
YNAVADTLEIINDTIRFQGSE O88871 441 5 -

Motif 10 width=20
Element Seqn Id St Int Rpt
PDAATQNRRFQFTQNQKKED O75899 751 288 -
PDAATQNRRFQFTQNQKKED O88871 750 288 -

Motif 11 width=25
Element Seqn Id St Int Rpt
RLEGLQSENHRLRMKITELDKDLEE O75899 787 16 -
RLEGLQSENHRLRMKITELDKDLEE O88871 786 16 -

Motif 12 width=21
Element Seqn Id St Int Rpt
KAILKNHLDQNPQLQWNTTEP O75899 851 39 -
KAILKNHLDQNPQLQWNTTEP O88871 850 39 -

Motif 13 width=21
Element Seqn Id St Int Rpt
DASCVSPCVSPTASPRHRHVP O75899 911 39 -
DASCVSPCVSPTASPRHRHVP O88871 910 39 -