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PR01154

Identifier
VIP1RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01154
No. of Motifs
8
Creation Date
23-MAY-1999
Title
Vasoactive intestinal peptide receptor 1 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00249 GPCRSECRETIN
PRINTS; PR00491 VASOACTVEIPR
INTERPRO; IPR001771
GCRDB; GCR_0397; GCR_0652; GCR_0774; GCR_0904; GCR_1888; GCR_1565

GCRDB; GCR_0369
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.   
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.  
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
 
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991). 
 
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
 
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
 
6. WATSON, S. AND ARKINSTALL, S.
Vasoactive intestinal polypeptide family.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.278-283.
 
7. WEI, Y. AND MOJSOV, S.
Tissue specific expression of different human receptor types for pituitary
adenylate cyclase activating polypeptide and vasoactive intestinal 
polypeptide: implications for their role in human physiology.
J.NEUROENDOCRINOL. 8 811-817 (1996). 
 
8. DELGADO, M., MARTINEZ, C., JOHNSON, M.C., GOMARIZ, R.P. AND GANEA, D.
Differential expression of vasoactive intestinal peptide receptors 1 and 2
J.NEUROIMMUNOL. 68 27-38 (1996). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that   
encompasses a wide range of functions (including various autocrine, para-   
crine and endocrine processes). They show considerable diversity at the   
sequence level, on the basis of which they can be separated into distinct   
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,   
but between which there is no statistically significant similarity in   
sequence [1]. The currently known clan members include the rhodopsin-like   
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating   
pheromone receptors, and the metabotropic glutamate receptor family.       
 
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid   
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-   
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
families: the secretin-like receptors thus bear their own unique `7TM'
signature.      
 
Vasoactive intestinal polypeptide (VIP) has a wide physiological profile.
In the periphery, it induces relaxation in smooth muscle; inhibits
secretion in certain tissues, but stimulates secretion in others; and
modulates activity of cells in the immune system [6]. In the CNS, it has a
range of both excitatory and inhibitory actions. VIP receptors are
distributed widely in the periphery, and occur throughout the gastro-
intestinal tract and genitourinary system, other smooth muscles and
secretory glands. In the CNS, they are found abundantly in, e.g. the cortex,
hippocampus and thalamus [6]. All VIP receptors activate adenylyl cyclase.
 
There are two structurally distinct receptors that recognise VIP peptides
and pituitary adenylate cyclase activating polypeptide (PACAP) with similar
affinities (PACAP/VIPR-1, PACAP/VIPR-2), as well as a specific receptor for
the PACAP peptide (PACAP-1). RNA transcripts for all three receptor types
are found in human heart, brain and adipose tissue [7]. VIPR-1 is 
constitutively expressed, while the expression of VIPR-2 is induced only
following stimulation through the TCR-associated CD3 complex [8]. VIP
induces the expression of the VIPR-2 gene in the absence of additional
stimuli. Differential expression and regulation of the two VIP receptors 
in T lymphocytes suggests different physiological roles in mediating the
immunomodulatory activities of VIP and related neuropeptides [8]. 
 
VIP1RECEPTOR is an 8-element fingerprint that provides a signature for 
vasoactive intestinal peptide type 1 receptors. The fingerprint was derived
from an initial alignment of 3 sequences: the motifs were drawn from 
conserved regions spanning the full alignment length, focusing on those
areas that characterise the type 1 VIP receptors but distinguish them from
the rest of the VIP receptor family - motifs 1-3 lie in the N-terminal 
region immediately preceding TM domain 1; motif 4 spans the loop between
TM domains 2 and 3; motif 5 resides in the loop between TM domains 3 and 4;
motif 6 spans the loop between TM domains 6 and 7; and motifs 7 and 8 reside
at the C-terminus. A single iteration on SPTR37_9f was required to reach 
convergence, no further sequences being identified beyond the starting set.
A single partial match was found, VIPR_CARAU, a goldfish type 1 VIP receptor
that matches motifs 4, 5, 7 and 8.
Summary Information
   3 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
833333333
700000000
600000000
500000000
400011011
300000000
200000000
12345678
True Positives
VIPR_HUMAN    VIPR_PIG      VIPR_RAT      
True Positive Partials
Codes involving 4 elements
VIPR_CARAU
Sequence Titles
VIPR_HUMAN  VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-R-1) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE II RECEPTOR) (PACAP TYPE II RECEPTOR) (PACAP-R-2) - HOMO SAPIENS (HUMAN). 
VIPR_PIG VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-R-1) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE II RECEPTOR) (PACAP TYPE II RECEPTOR) (PACAP-R-2) - SUS SCROFA (PIG).
VIPR_RAT VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-R-1) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE II RECEPTOR) (PACAP TYPE II RECEPTOR) (PACAP-R-2) - RATTUS NORVEGICUS (RAT).

VIPR_CARAU VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR (VIP-R) (VIP RECEPTOR) - CARASSIUS AURATUS (GOLDFISH).
Scan History
SPTR37_9f  1  50   NSINGLE    
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
ECDYLQMIKVQHKQCLEEAQLE VIPR_PIG 37 37 -
ECDYVQMIEVQHKQCLEEAQLE VIPR_HUMAN 36 36 -
ECEYLQLIEIQRQQCLEEAQLE VIPR_RAT 36 36 -

Motif 2 width=17
Element Seqn Id St Int Rpt
WPATPRGQVVVLACPLI VIPR_PIG 74 15 -
WPATPRGQVVVLACPLI VIPR_HUMAN 73 15 -
WPTTPRGQAVVLDCPLI VIPR_RAT 73 15 -

Motif 3 width=21
Element Seqn Id St Int Rpt
LEPGPYPIACGMDDKASGLDE VIPR_PIG 114 23 -
LEPGPYPIACGLDDKAASLDE VIPR_HUMAN 113 23 -
LEPGPYHIACGLNDRASSLDE VIPR_RAT 113 23 -

Motif 4 width=15
Element Seqn Id St Int Rpt
FDSEESDHCSKGSVG VIPR_PIG 201 66 -
FDSGESDQCSEGSVG VIPR_HUMAN 200 66 -
FNSGEIDHCSEASVG VIPR_RAT 201 67 -

Motif 5 width=14
Element Seqn Id St Int Rpt
VSFFSERKYFWGYI VIPR_PIG 247 31 -
VSFFSERKYFWGYI VIPR_HUMAN 246 31 -
VSFFSERKYFWGYI VIPR_RAT 247 31 -

Motif 6 width=13
Element Seqn Id St Int Rpt
FFPDNFKAEVKMV VIPR_PIG 360 99 -
FFPDNFKPEVKMV VIPR_HUMAN 359 99 -
FFPDNFKAQVKMV VIPR_RAT 361 100 -

Motif 7 width=23
Element Seqn Id St Int Rpt
RWHQQGVLGWDSKYQHPSGGSNG VIPR_PIG 406 33 -
RWHLQGVLGWNPKYRHPSGGSNG VIPR_HUMAN 405 33 -
RWHLQGVLGWSSKSQHPWGGSNG VIPR_RAT 407 33 -

Motif 8 width=24
Element Seqn Id St Int Rpt
CSTQVSMLTRVSPSARRSSSFQAE VIPR_PIG 431 2 -
CSTQVSMLTRVSPGARRSSSFQAE VIPR_HUMAN 430 2 -
CSTQVSMLTRVSPSARRSSSFQAE VIPR_RAT 432 2 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
ECDYLQMIKVQHKQCLEEAQLE VIPR_PIG 37 37 -
ECDYVQMIEVQHKQCLEEAQLE VIPR_HUMAN 36 36 -
ECEYLQLIEIQRQQCLEEAQLE VIPR_RAT 36 36 -

Motif 2 width=17
Element Seqn Id St Int Rpt
WPATPRGQVVVLACPLI VIPR_PIG 74 15 -
WPATPRGQVVVLACPLI VIPR_HUMAN 73 15 -
WPTTPRGQAVVLDCPLI VIPR_RAT 73 15 -

Motif 3 width=21
Element Seqn Id St Int Rpt
LEPGPYPIACGMDDKASGLDE VIPR_PIG 114 23 -
LEPGPYPIACGLDDKAASLDE VIPR_HUMAN 113 23 -
LEPGPYHIACGLNDRASSLDE VIPR_RAT 113 23 -

Motif 4 width=15
Element Seqn Id St Int Rpt
FDSEESDHCSKGSVG VIPR_PIG 201 66 -
FDSGESDQCSEGSVG VIPR_HUMAN 200 66 -
FNSGEIDHCSEASVG VIPR_RAT 201 67 -

Motif 5 width=14
Element Seqn Id St Int Rpt
VSFFSERKYFWGYI VIPR_PIG 247 31 -
VSFFSERKYFWGYI VIPR_HUMAN 246 31 -
VSFFSERKYFWGYI VIPR_RAT 247 31 -

Motif 6 width=13
Element Seqn Id St Int Rpt
FFPDNFKAEVKMV VIPR_PIG 360 99 -
FFPDNFKPEVKMV VIPR_HUMAN 359 99 -
FFPDNFKAQVKMV VIPR_RAT 361 100 -

Motif 7 width=23
Element Seqn Id St Int Rpt
RWHQQGVLGWDSKYQHPSGGSNG VIPR_PIG 406 33 -
RWHLQGVLGWNPKYRHPSGGSNG VIPR_HUMAN 405 33 -
RWHLQGVLGWSSKSQHPWGGSNG VIPR_RAT 407 33 -

Motif 8 width=24
Element Seqn Id St Int Rpt
CSTQVSMLTRVSPSARRSSSFQAE VIPR_PIG 431 2 -
CSTQVSMLTRVSPGARRSSSFQAE VIPR_HUMAN 430 2 -
CSTQVSMLTRVSPSARRSSSFQAE VIPR_RAT 432 2 -