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PR01146

Identifier
GPR1ORPHANR  [View Relations]  [View Alignment]  
Accession
PR01146
No. of Motifs
9
Creation Date
14-MAR-1999
Title
GPR1 orphan receptor signature 
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN

INTERPRO; IPR002275
GCRDB; GCR_1172
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. MARCHESE, A., DOCHERTY, J.M., NGUYEN, T., HEIBER, M., CHENG, R., 
HENG, H.H., TSUI, L.C., SHI, X., GEORGE, S.R. AND O'DOWD, B.F.
Cloning of human genes encoding novel G protein-coupled receptors.
GENOMICS 23:609-618(1994). 
 
7. MARCHESE, A., CHENG, R., LEE, M.C., PORTER, C.A., HEIBER, M., GOODMAN, M.,
GEORGE, S.R. AND O'DOWD, B.F.
Mapping studies of two G protein-coupled receptor genes: an amino acid
difference may confer a functional variation between a human and rodent
receptor.
BIOCHEM.BIOPHYS.RES.COMMUN. 205 1952-1958 (1994). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Several 7TM receptors have been cloned but their endogenous ligands are
unknown; these have been termed orphan receptors. Several novel human genes 
encoding GPCRs that most closely resemble peptide binding receptors have
been isolated and characterised [6]. Gene GPR1, which is intronless in the
coding region, encodes a receptor that shares identity in the TM regions
with the opioid receptors. GPR1 transcripts are expressed in the human
hippocampus [6]. By contrast, the rat GPR1 gene is not expressed in
hippocampus, demonstrating a functional variation for this receptor in 
these two species [7]. 
 
GPR1ORPHANR is a 9-element fingerprint that provides a signature for the
GPR1 orphan receptor. The fingerprint was derived from an initial alignment
of 2 sequences: the motifs were drawn from short conserved regions spanning
the full alignment length, focusing on those sections that characterise the
GPR1 orphan receptor but distinguish it from related members of the 
rhodopsin-like superfamily - motifs 1 and 2 reside at the N-terminus; motif
3 lies in the second cytoplasmic loop; motifs 4 and 5 span the second 
external loop; motif 6 spans the third cytoplasmic loop and the N-terminus
of TM domain 6; motif 7 lies in the third external loop; and motifs 8 and 9
reside at the C-terminus. A single iteration on SPTR37_9f was required to 
reach convergence, no further sequences being identified beyond the starting
set.
Summary Information
2 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9222222222
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
GPR1_HUMAN    GPR1_RAT      
Sequence Titles
GPR1_HUMAN  Probable G-protein coupled receptor 1 - Homo sapiens (Human). 
GPR1_RAT Probable G-protein coupled receptor 1 - Rattus norvegicus (Rat).
Scan History
SPTR37_9f  1  3    NSINGLE    
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
EDLEETLFEEFENYSYDLDYYS GPR1_HUMAN 2 2 -
EVSREMLFEELDNYSYALEYYS GPR1_RAT 2 2 -

Motif 2 width=15
Element Seqn Id St Int Rpt
ESDLEEKVQLGVVHW GPR1_HUMAN 25 1 -
EPDAEENVYPGIVHW GPR1_RAT 25 1 -

Motif 3 width=17
Element Seqn Id St Int Rpt
HLIHPVLSHRHRTLKNS GPR1_HUMAN 138 98 -
HLIHPGLSHPHRTLKNS GPR1_RAT 138 98 -

Motif 4 width=16
Element Seqn Id St Int Rpt
GGPALYFRDTVEFNNH GPR1_HUMAN 169 14 -
GGPTLYFRDTVEVNNR GPR1_RAT 169 14 -

Motif 5 width=13
Element Seqn Id St Int Rpt
LTLIRHHVLTWVK GPR1_HUMAN 198 13 -
LTLMRHHVLTWVK GPR1_RAT 196 11 -

Motif 6 width=16
Element Seqn Id St Int Rpt
KKRTVLISSRHFWTIL GPR1_HUMAN 234 23 -
KKQNILISSKHLWMIL GPR1_RAT 232 23 -

Motif 7 width=13
Element Seqn Id St Int Rpt
LFSIWELTIHHNS GPR1_HUMAN 264 14 -
LFSIWELSIHHNS GPR1_RAT 262 14 -

Motif 8 width=18
Element Seqn Id St Int Rpt
ISKKFQARFRSSVAEILK GPR1_HUMAN 307 30 -
ISKKFQARFRASVAEVLK GPR1_RAT 305 30 -

Motif 9 width=22
Element Seqn Id St Int Rpt
LWEVSCSGTVSEQLRNSETKNL GPR1_HUMAN 327 2 -
LWEASCSGTVSEQLRSAETKSL GPR1_RAT 325 2 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
EDLEETLFEEFENYSYDLDYYS GPR1_HUMAN 2 2 -
EVSREMLFEELDNYSYALEYYS GPR1_RAT 2 2 -

Motif 2 width=15
Element Seqn Id St Int Rpt
ESDLEEKVQLGVVHW GPR1_HUMAN 25 1 -
EPDAEENVYPGIVHW GPR1_RAT 25 1 -

Motif 3 width=17
Element Seqn Id St Int Rpt
HLIHPVLSHRHRTLKNS GPR1_HUMAN 138 98 -
HLIHPGLSHPHRTLKNS GPR1_RAT 138 98 -

Motif 4 width=16
Element Seqn Id St Int Rpt
GGPALYFRDTVEFNNH GPR1_HUMAN 169 14 -
GGPTLYFRDTVEVNNR GPR1_RAT 169 14 -

Motif 5 width=13
Element Seqn Id St Int Rpt
LTLIRHHVLTWVK GPR1_HUMAN 198 13 -
LTLMRHHVLTWVK GPR1_RAT 196 11 -

Motif 6 width=16
Element Seqn Id St Int Rpt
KKRTVLISSRHFWTIL GPR1_HUMAN 234 23 -
KKQNILISSKHLWMIL GPR1_RAT 232 23 -

Motif 7 width=13
Element Seqn Id St Int Rpt
LFSIWELTIHHNS GPR1_HUMAN 264 14 -
LFSIWELSIHHNS GPR1_RAT 262 14 -

Motif 8 width=18
Element Seqn Id St Int Rpt
ISKKFQARFRSSVAEILK GPR1_HUMAN 307 30 -
ISKKFQARFRASVAEVLK GPR1_RAT 305 30 -

Motif 9 width=22
Element Seqn Id St Int Rpt
LWEVSCSGTVSEQLRNSETKNL GPR1_HUMAN 327 2 -
LWEASCSGTVSEQLRSAETKSL GPR1_RAT 325 2 -