SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01128

Identifier
EMR1HORMONER  [View Relations]  [View Alignment]  
Accession
PR01128
No. of Motifs
9
Creation Date
22-MAY-1999
Title
EMR1 hormone receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00249 GPCRSECRETIN

INTERPRO; IPR001740
GCRDB; GCR_1309
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994). 
 
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.
Molecular cloning and expression of a cDNA encoding the secretin receptor
EMBO J. 10 1635-1641 (1991).
 
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H., 
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor
SCIENCE 254 1022-1024 (1991).
 
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F., 
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
 
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
 
6. MCKNIGHT, A.J., MACFARLANE, A.J., DRI, P., TURLEY, L., WILLIS, A.C.
AND GORDON, S.
Molecular cloning of F4/80, a murine macrophage-restricted cell surface
glycoprotein with homology to the G protein-linked transmembrane 7 hormone
receptor family.
J.BIOL.CHEM. 271 486-489 (1996).
 
7. BAUD, V., CHISSOE, S.L., VIEGAS-PEQUIGNOT, E., DIRIONG, S., N'GUYEN, V.C.,
ROE, B.A. AND LIPINSKI, M.
EMR1, an unusual member in the family of hormone receptors with seven
transmembrane segments.
GENOMICS 26 334-344 (1995). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a
manner reminiscent of the rhodopsins and other receptors believed to inter-
act with G proteins. However, while a similar 3D framework has been
proposed to account for this, there is no significant sequence similarity
between these families: the secretin-like receptors thus bear their own
unique `7TM' signature.
 
F4/80 is a monoclonal antibody that recognises a murine macrophage-
restricted cell surface glycoprotein and has been extensively used to
characterise macrophage populations in a wide range of immunological studies
[6]. Little is known about its possible role in macrophage differentiation
and function. The sequence of the F4/80 protein is similar to two protein
superfamilies: the N-terminal region contains seven epidermal growth factor
(EGF)-like domains, while the C-terminal region contains seven hydrophobic
regions whose signature is consistent with membership of the secretin-like
superfamily of GPCRs. The EGF and GPCR domains are separated from each other
by a serine/threonine-rich domain, a feature reminiscent of mucin-like,
single-span, integral membrane glycoproteins with adhesive properties [7].
Because of its unique characteristics, this putative EGF module-containing, 
mucin-like hormone receptor has been named EMR1 [7]. The EMR1 gene has been
localised to human chromosome 19p13.3 [7]. 
 
EMR1HORMONER is a 9-element fingerprint that provides a signature for EMR1
hormone receptors. The fingerprint was derived from an initial alignment of
2 sequences: the motifs were drawn from conserved regions spanning the 
C-terminal half of the alignment, focusing on those sections that 
characterise the EMR1 hormone receptors but distinguish them from the rest
of the secretin-like superfamily - motifs 1-4 lie immediately N-terminal to
TM domain 1; motif 5 spans the loop between TM domains 1 and 2 and the
N-terminal portion of TM domain 2; motif 6 lies in the loop between TM 
domains 3 and 4; motif 7 spans the loop between TM domains 5 and 6, and the
N-terminal portion of TM domain 6; and motif 9 spans the C-terminus. A 
single iteration on SPTR37_9f was required to reach convergence, no further 
sequences being identified beyond the starting set. Two partial matches were
also found, both leucocyte antigen CD97 sequences that match motifs 5 and 8.
Summary Information
   2 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
9222222222
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000020020
123456789
True Positives
EMR1_HUMAN    EMR1_MOUSE    
True Positive Partials
Codes involving 2 elements
CD97_HUMAN O00718
Sequence Titles
EMR1_HUMAN  CELL SURFACE GLYCOPROTEIN EMR1 PRECURSOR (EMR1 HORMONE RECEPTOR) - HOMO SAPIENS (HUMAN). 
EMR1_MOUSE CELL SURFACE GLYCOPROTEIN EMR1 PRECURSOR (EMR1 HORMONE RECEPTOR) (CELL SURFACE GLYCOPROTEIN F4/80) - MUS MUSCULUS (MOUSE).

CD97_HUMAN LEUCOCYTE ANTIGEN CD97 PRECURSOR - HOMO SAPIENS (HUMAN).
O00718 CD97 - HOMO SAPIENS (HUMAN).
Scan History
SPTR37_9f  1  50   NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
NSRVVGGIMTGEKKDGFS EMR1_HUMAN 512 512 -
NSRVVGGTVTGEKKEDFS EMR1_MOUSE 557 557 -

Motif 2 width=20
Element Seqn Id St Int Rpt
YTLENVQPKQKFERPICVSW EMR1_HUMAN 534 4 -
YTLQHIQPKQKSERPICVSW EMR1_MOUSE 579 4 -

Motif 3 width=19
Element Seqn Id St Int Rpt
WSTDVKGGRWTSFGCVILE EMR1_HUMAN 553 -1 -
WNTDVEDGRWTPSGCEIVE EMR1_MOUSE 598 -1 -

Motif 4 width=22
Element Seqn Id St Int Rpt
NQMANLAVIMASGELTMDFSLY EMR1_HUMAN 582 10 -
NRMANLAIIMASGELTMEFSLY EMR1_MOUSE 627 10 -

Motif 5 width=18
Element Seqn Id St Int Rpt
CRSIRNHNTYLHLHLCVC EMR1_HUMAN 627 23 -
CRAVQNHNTYMHLHLCVC EMR1_MOUSE 672 23 -

Motif 6 width=20
Element Seqn Id St Int Rpt
RNLKVVNYFSSRNIKMLHIC EMR1_HUMAN 695 50 -
RNLKVVNYFSSRNIKMLHLC EMR1_MOUSE 740 50 -

Motif 7 width=15
Element Seqn Id St Int Rpt
SASVQPQGYGMHNRC EMR1_HUMAN 728 13 -
SASVQPRGYGMHNRC EMR1_MOUSE 773 13 -

Motif 8 width=22
Element Seqn Id St Int Rpt
EVSTLKDTRLLTFKAFAQLFIL EMR1_HUMAN 786 43 -
EVSKLKDTRLLTFKAIAQIFIL EMR1_MOUSE 831 43 -

Motif 9 width=25
Element Seqn Id St Int Rpt
TGKTKPSSQSQTSRILLSSMPSASK EMR1_HUMAN 860 52 -
TRKTDLSSHSQTSGILLSSMPSTSK EMR1_MOUSE 905 52 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
NSRVVGGIMTGEKKDGFS EMR1_HUMAN 512 512 -
NSRVVGGTVTGEKKEDFS EMR1_MOUSE 557 557 -

Motif 2 width=20
Element Seqn Id St Int Rpt
YTLENVQPKQKFERPICVSW EMR1_HUMAN 534 4 -
YTLQHIQPKQKSERPICVSW EMR1_MOUSE 579 4 -

Motif 3 width=19
Element Seqn Id St Int Rpt
WSTDVKGGRWTSFGCVILE EMR1_HUMAN 553 -1 -
WNTDVEDGRWTPSGCEIVE EMR1_MOUSE 598 -1 -

Motif 4 width=22
Element Seqn Id St Int Rpt
NQMANLAVIMASGELTMDFSLY EMR1_HUMAN 582 10 -
NRMANLAIIMASGELTMEFSLY EMR1_MOUSE 627 10 -

Motif 5 width=18
Element Seqn Id St Int Rpt
CRSIRNHNTYLHLHLCVC EMR1_HUMAN 627 23 -
CRAVQNHNTYMHLHLCVC EMR1_MOUSE 672 23 -

Motif 6 width=20
Element Seqn Id St Int Rpt
RNLKVVNYFSSRNIKMLHIC EMR1_HUMAN 695 50 -
RNLKVVNYFSSRNIKMLHLC EMR1_MOUSE 740 50 -

Motif 7 width=15
Element Seqn Id St Int Rpt
SASVQPQGYGMHNRC EMR1_HUMAN 728 13 -
SASVQPRGYGMHNRC EMR1_MOUSE 773 13 -

Motif 8 width=22
Element Seqn Id St Int Rpt
EVSTLKDTRLLTFKAFAQLFIL EMR1_HUMAN 786 43 -
EVSKLKDTRLLTFKAIAQIFIL EMR1_MOUSE 831 43 -

Motif 9 width=25
Element Seqn Id St Int Rpt
TGKTKPSSQSQTSRILLSSMPSASK EMR1_HUMAN 860 52 -
TRKTDLSSHSQTSGILLSSMPSTSK EMR1_MOUSE 905 52 -