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PR01091

Identifier
OREXINPP  [View Relations]  [View Alignment]  
Accession
PR01091
No. of Motifs
4
Creation Date
25-FEB-1999  (UPDATE 07-JUN-1999)
Title
Prepro-orexin signature
Database References

PRODOM; PD020321
INTERPRO; IPR001704
Literature References
1. DATE, Y., UETA, Y., YAMASHITA, H., YAMAGUCHI, H., MATSUKURA, S.,
KANGAWA, K., SAKURAI, T., YANAGISAWA, M. AND NAKAZATO, M.
Orexins, orexigenic hypothalamic peptides, interact with autonomic,
neuroendocrine and neuroregulatory systems.
PROC.NATL.ACAD.SCI.U.S.A. 96 748-753 (1999).
 
2. DE LECEA, L., KILDUFF, T.S., PEYRON, C., GAO, X.-B., FOYE, P.E.,
DANIELSON, P.E., FUKUHARA, C., BATTENBERG, E.L.F., GAUTVIK, V.T.,
BARTLETT, F.S. II, FRANKEL, W.N., VAN DEN POL, A.N., BLOOM, F.E., 
GAUTVIK K.M. AND SUTCLIFFE J.G.
The hypocretins: hypothalamus-specific peptides with neuroexcitatory
activity.
PROC.NATL.ACAD.SCI.U.S.A. 95 322-327 (1998).
 
3. SAKURAI, T., AMEMIYA, A., ISHII, M., MATSUZAKI, I., CHEMELLI, R.M.,
TANAKA, H., WILLIAMS, S.C., RICHARDSON, J.A., KOZLOWSKI, G.P., WILSON, S.,
ARCH, J.R., BUCKINGHAM, R.E., HAYNES, A.C., CARR, S.A., ANNAN, R.S., 
MCNULTY, D.E., LIU, W.S., TERRETT, J.A., ELSHOURBAGY, N.A., BERGSMA, D.J. 
AND YANAGISAWA, M.
Orexins and orexin receptors: a family of hypothalamic neuropeptides and G
protein-coupled receptors that regulate feeding behavior.
CELL 92 573-585 (1998).

Documentation
Orexins (also known as hypocretins) are recently identified neuropeptides
that are specifically localised to the hypothalamus. They are thought to
interact with autonomic, neurendocrine and neuroregulatory systems, and play
an important role in the regulation of feeding behaviour [1,2]. When applied
to hypothalamic neurones, these peptides are neuroexcitatory, which action
is probably mediated by their binding to a new family of G protein-coupled
receptors (orexin receptors 1 and 2), which were previously orphan [3].
 
To date, two orexins have been characterised (orexin-A and -B), both encoded
by a single mRNA transcript (prepro-orexin): orexin-A is a 33-residue
peptide with two intramolecular disulphide bonds in the N-terminal region;
and orexin-B is a linear 28-residue peptide. These peptides have 46%
identity at the amino acid sequence level, and show some similarity to the
glucagon/vasoactive intestinal polypeptide/secretin peptide family.
 
OREXINPP is a 4-element fingerprint that provides a signature for prepro-
orexin. The fingerprint was derived from an initial alignment of 2 sequences: 
the motifs were drawn from the central portion of the alignment, which 
characterises prepro-orexins and distinguishes them from the secretin-
related peptides (to which greatest similarity is shown in the C-terminal
region). Two iterations on OWL31.1 were required to reach convergence, at
which point a true set comprising 4 sequences was identified. 
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
44444
30000
20000
1234
True Positives
O43612        O55232        O55241        O77668        
Sequence Titles
O43612      PREPRO-OREXIN - HOMO SAPIENS (HUMAN).         
O55232 PREPRO-OREXIN - RATTUS NORVEGICUS (RAT).
O55241 HYPOCRETIN (PREPRO-OREXIN) - MUS MUSCULUS (MOUSE).
O77668 PREPRO-OREXIN PRECURSOR - SUS SCROFA (PIG).
Scan History
OWL31_1    2  150  NSINGLE    
SPTR37_9f 2 10 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
QPLPDCCRQKTCSCRLYE AF075241 34 34 -
QPLPDCCRQKTCSCRLYE AF041240 34 34 -

Motif 2 width=19
Element Seqn Id St Int Rpt
LLHGAGNHAAGILTLGKRR AF075241 52 0 -
LLHGAGNHAAGILTLGKRR AF041240 52 0 -

Motif 3 width=11
Element Seqn Id St Int Rpt
LQGRLQRLLQA AF075241 76 5 -
LQGRLQRLLQA AF041240 76 5 -

Motif 4 width=17
Element Seqn Id St Int Rpt
GNHAAGILTMGRRAGAE AF075241 88 1 -
GNHAAGILTMGRRAGAE AF041240 88 1 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
QPLPDCCRQKTCSCRLYE O43612 34 34 -
QPLPDCCRQKTCSCRLYE O77668 34 34 -
QPLPDCCRQKTCSCRLYE O55241 33 33 -
QPLPDCCRQKTCSCRLYE O55232 33 33 -

Motif 2 width=19
Element Seqn Id St Int Rpt
LLHGAGNHAAGILTLGKRR O43612 52 0 -
LLHGAGNHAAGILTLGKRR O77668 52 0 -
LLHGAGNHAAGILTLGKRR O55241 51 0 -
LLHGAGNHAAGILTLGKRR O55232 51 0 -

Motif 3 width=11
Element Seqn Id St Int Rpt
LQGRLQRLLQA O43612 76 5 -
LQGRLQRLLQA O77668 76 5 -
LQGRLQRLLQA O55241 75 5 -
LQGRLQRLLQA O55232 75 5 -

Motif 4 width=17
Element Seqn Id St Int Rpt
GNHAAGILTMGRRAGAE O43612 88 1 -
GNHAAGILTMGRRAGAE O77668 88 1 -
GNHAAGILTMGRRAGAE O55241 87 1 -
GNHAAGILTMGRRAGAE O55232 87 1 -