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PR01076

Identifier
CALDESMON  [View Relations]  [View Alignment]  
Accession
PR01076
No. of Motifs
7
Creation Date
16-JAN-1999  (UPDATE 06-JUN-1999)
Title
Caldesmon signature
Database References

INTERPRO; IPR000075
Literature References
1. HUMPHREY, M.B., HERRERA-SOSA, H., GONZALEZ, G., LEE, R. AND BRYAN, J.
Cloning of cDNAs encoding human caldesmons.
GENE 112 197-204 (1992). 
 
2. BRYAN, J. AND LEE, R.
Sequence of an avian non-muscle caldesmon.
J.MUSCLE RES.CELL.MOTIL. 12 372-375 (1991)

Documentation
Caldesmon (CDM) is an actin- and myosin-binding protein implicated in the
regulation of actomyosin interactions in smooth muscle and non-muscle cells,
possibly acting as a bridge between myosin and actin filaments) [1]. CDM is
believed to be an elongated molecule, with an N-terminal myosin/calmodulin-
binding domain and a C-terminal tropomyosin/actin/calmodulin-binding domain,
separated by a 40nm-long central helix [1].
 
A high-molecular-weight form of CDM is predominantly expressed in smooth
muscles, while a low-molecular-weight form is widely distributed in non-
muscle tissues and cells (the protein is not expressed in skeletal muscle
or heart). 
 
A short CDM has been cloned from a chicken gizzard library [2]. The 
predicted protein contains 524 amino acids, with molecular mass ~60Kda.
The expressed protein binds to F-actin and is retained on calmodulin-
Sepharose in the presence of Ca2+ [2]. Like the human non-muscle form [1],
this CDM is identical to the smooth muscle protein at its N- and C-termini,
but is missing 232 amino acids from the centre. Lack of this central
segment, which is thought to be helical, renders the non-muscle protein
~35nm shorter than smooth muscle CDM [2].
 
CALDESMON is a 7-element fingerprint that provides a signature for
caldesmons. The fingerprint was derived from an initial alignment of 4
sequences: the motifs were drawn from conserved regions spanning the
C-terminal portion of the alignment - motif 3 encodes an actin-binding 
region; and motif 5 includes a calmodulin-binding domain. Two iterations 
on OWL31.1 were required to reach convergence, at which point a true set
comprising 11 sequences was identified.
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
74444444
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
CALD_CHICK    CALD_HUMAN    CALD_RAT      Q28708        
Sequence Titles
CALD_CHICK  CALDESMON (CDM) - GALLUS GALLUS (CHICKEN).    
CALD_HUMAN CALDESMON (CDM) - HOMO SAPIENS (HUMAN).
CALD_RAT NON-MUSCLE CALDESMON (CDM) (L-CALDESMON) - RATTUS NORVEGICUS (RAT).
Q28708 L-CALDESMON - ORYCTOLAGUS CUNICULUS (RABBIT).
Scan History
OWL31_1    2  100  NSINGLE    
SPTR37_9f 2 5 NSINGLE
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
EKRQKMPEDGLSEDKKPFKCF CALD_RAT 356 356 -
EKRQKVPEDGVSEEKKPFKCF CALD_CHICK 576 576 -
EKRQKMPEDGVSEEKKPFKCF CALD_MELGA 79 79 -
EKRQKMPEDGLSDDKKPFKCF CALD_HUMAN 617 617 -

Motif 2 width=24
Element Seqn Id St Int Rpt
FSPKGSSLKIEERAEFLNKSAQKS CALD_CHICK 596 -1 -
FTPKGSSLKIEERAEFLNKSVQKS CALD_RAT 376 -1 -
FSPKGSSLKIEERAEFLNKSAQKS CALD_MELGA 99 -1 -
FTPKGSSLKIEERAEFLNKSVQKS CALD_HUMAN 637 -1 -

Motif 3 width=21
Element Seqn Id St Int Rpt
GVKSTHQAAVVSKIDSRLEQY CALD_RAT 400 0 -
GMKPAHTTAVVSKIDSRLEQY CALD_MELGA 123 0 -
GVKSTHQAAIVSKIDSRLEQY CALD_HUMAN 662 1 -
GMKPAHTTAVVSKIDSRLEQY CALD_CHICK 620 0 -

Motif 4 width=22
Element Seqn Id St Int Rpt
GTKSAKPTKPAASDLPVPAEGV CALD_HUMAN 688 5 -
GNKAAKPAKPAASDLPVPAEGV CALD_CHICK 646 5 -
GNKAAKPAKPAASDLPVPAEGV CALD_MELGA 149 5 -
GTKASKPMKPAASDLPVPAEGV CALD_RAT 426 5 -

Motif 5 width=16
Element Seqn Id St Int Rpt
RNIKSMWEKGNVFSSP CALD_MELGA 171 0 -
RNIKSMWEKGNVFSSP CALD_HUMAN 710 0 -
RNIKSMWEKGSVFSSP CALD_RAT 448 0 -
RNIKSMWEKGNVFSSP CALD_CHICK 668 0 -

Motif 6 width=22
Element Seqn Id St Int Rpt
GTPNKETAGLKVGVSSRINEWL CALD_CHICK 687 3 -
GTPNKETAGLKVGVSSRINEWL CALD_MELGA 190 3 -
GTPNKETAGLKVGVSSRINEWL CALD_HUMAN 729 3 -
GTPNKETAGLKVGVSSRINEWL CALD_RAT 467 3 -

Motif 7 width=21
Element Seqn Id St Int Rpt
TKTPDGNKSPAPKPSDLRPGD CALD_HUMAN 751 0 -
TKTPEGNKSPAPKPSDLRPGD CALD_CHICK 709 0 -
TKSPDGNKSPAPKPSDLRPGD CALD_RAT 489 0 -
TKTPEGNKSPAPKPSDLRPGD CALD_MELGA 212 0 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
EKRQKMPEDGLSDDKKPFKCF CALD_HUMAN 617 617 -
EKRQKMPEDGLSDDKKPFKCF Q28708 358 358 -
EKRQKMPEDGLSEDKKPFKCF CALD_RAT 356 356 -
EKRQKVPEDGVSEEKKPFKCF CALD_CHICK 576 576 -

Motif 2 width=24
Element Seqn Id St Int Rpt
FTPKGSSLKIEERAEFLNKSVQKS CALD_HUMAN 637 -1 -
FTPKGSSLKIEERAEFLNKSVQKS Q28708 378 -1 -
FTPKGSSLKIEERAEFLNKSVQKS CALD_RAT 376 -1 -
FSPKGSSLKIEERAEFLNKSAQKS CALD_CHICK 596 -1 -

Motif 3 width=21
Element Seqn Id St Int Rpt
GVKSTHQAAIVSKIDSRLEQY CALD_HUMAN 662 1 -
GVKSTHQAAVVSKIDSRLEQY Q28708 402 0 -
GVKSTHQAAVVSKIDSRLEQY CALD_RAT 400 0 -
GMKPAHTTAVVSKIDSRLEQY CALD_CHICK 620 0 -

Motif 4 width=22
Element Seqn Id St Int Rpt
GTKSAKPTKPAASDLPVPAEGV CALD_HUMAN 688 5 -
GTKAAKSTKPAASDLPVPAEGV Q28708 428 5 -
GTKASKPMKPAASDLPVPAEGV CALD_RAT 426 5 -
GNKAAKPAKPAASDLPVPAEGV CALD_CHICK 646 5 -

Motif 5 width=16
Element Seqn Id St Int Rpt
RNIKSMWEKGNVFSSP CALD_HUMAN 710 0 -
RNIKSMWEKGNVFSAP Q28708 450 0 -
RNIKSMWEKGSVFSSP CALD_RAT 448 0 -
RNIKSMWEKGNVFSSP CALD_CHICK 668 0 -

Motif 6 width=22
Element Seqn Id St Int Rpt
GTPNKETAGLKVGVSSRINEWL CALD_HUMAN 729 3 -
GTPNKETAGLKVGVSSRINEWL Q28708 469 3 -
GTPNKETAGLKVGVSSRINEWL CALD_RAT 467 3 -
GTPNKETAGLKVGVSSRINEWL CALD_CHICK 687 3 -

Motif 7 width=21
Element Seqn Id St Int Rpt
TKTPDGNKSPAPKPSDLRPGD CALD_HUMAN 751 0 -
TKTPDGSKSPAPKPSDLRPGD Q28708 491 0 -
TKSPDGNKSPAPKPSDLRPGD CALD_RAT 489 0 -
TKTPEGNKSPAPKPSDLRPGD CALD_CHICK 709 0 -