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PR01060

Identifier
C3ANPHYLTXNR  [View Relations]  [View Alignment]  
Accession
PR01060
No. of Motifs
10
Creation Date
26-JAN-1999  (UPDATE 06-JUN-1999)
Title
C3A-anaphylatoxin receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01104 ANPHYLATOXNR
INTERPRO; IPR001644
GCRDB; GCR_1052; GCR_1916; GCR_1994; GCR_1303; GCR_1304; GCR_2591
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL,
C5a anaphylatoxin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp71-73.
 
7. AMES, R.S., LI, Y., SARAU, H.M., NUTHULAGANTI, P., FOLEY, J.J., 
ELLIS, C., ZENG, Z., SU, K., JUREWICZ, A.J., HERTZBERG, R.P., BERGSMA, D.J.
AND KUMAR, C.
Molecular cloning AND characterization of the human anaphylatoxin C3a receptor.
J.BIOL.CHEM. 271 20231-20234 (1996). 
 
8. CRASS, T., RAFFETSEDER, U., MARTIN, U., GROVE, M., KLOS, A., KOHL, J.
AND BAUTSCH, W.
Expression cloning of the human C3a anaphylatoxin receptor (C3aR) from
differentiated U-937 cells.
EUR.J.IMMUNOL. 26 1944-1950 (1996). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
An orphan GPCR with 37% nucleotide identity to the C5a receptor [6] has 
been identified [7]. By contrast with C5aR and other GPCRs, a novel feature
of the gene product is the presence of an unusually large predicted extra-
cellular loop, containing in excess of 160 amino acid residues, between
TM domains 4 and 5 [7]. In human tissues, expression of mRNA for this 
receptor is similar to, but distinct from C5aR expression [7]. Although
there are some differences, transcripts for both receptors are detected
in tissues throughout the body and the central nervous system [7]. This
novel gene has been shown to encode the human anaphylatoxin C3a receptor 
[7]. The C3aR appears to be widely expressed in different lymphoid tissues,
providing evidence for a central role of the C3a anaphylatoxin in 
inflammatory processes [8]. 
 
C3ANPHYLTXNR is a 10-element fingerprint that provides a signature for 
anaphylatoxin C3a receptors. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from short conserved 
regions spanning the full alignment length, focusing on those sections
that characterise the C3a receptors but distinguish them from the rest of
the rhodopsin-like superfamily - motifs 1 and 2 reside at the N-terminus;
motif 3 spans the first cytoplasmic loop; motif 4 spans the C-terminus of 
putative TM domain 2, leading into the first external loop; motifs 5 and 6
lie in the second external loop; motif 7 spans the third cytoplasmic loop;
motif 8 spans the C-terminus of TM domain 6, leading into the third external
loop; motif 9 spans the C-terminus of the third external loop and the
N-terminus of TM domain 7; and motif 10 resides at the C-terminus. Two
iterations on OWL31.1 were required to reach convergence, at which point
a true set comprising 7 sequences was identified.
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
105555555555
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
C3AR_HUMAN    C3AR_MOUSE    O43771        O55197        
O88680
Sequence Titles
C3AR_HUMAN  C3a anaphylatoxin chemotactic receptor (C3a-R) (C3AR) - Homo sapiens (Human). 
C3AR_MOUSE C3a anaphylatoxin chemotactic receptor (C3a-R) (C3AR) (Complement component 3a receptor 1) - Mus musculus (Mouse).
O43771 C3A ANAPHYLATOXIN RECEPTOR - HOMO SAPIENS (HUMAN).
O55197 ANAPHYLATOXIN C3A RECEPTOR - RATTUS NORVEGICUS (RAT).
O88680 C3A RECEPTOR - CAVIA PORCELLUS (GUINEA PIG).
Scan History
OWL31_1    2  75   NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
SFSAETNSTDLLSQPWNEP HSU62027 3 3 -
SFDADTNSTDLHSRPLFQP MMU97537 3 3 -
SFTADTNSTDLHSRPLFKP JC5835 3 3 -
SFDADTNSTDLHSRPLFQP C3AR_MOUSE 3 3 -
SFSAETNSTDLLSQPWNEP C3AR_HUMAN 3 3 -

Motif 2 width=15
Element Seqn Id St Int Rpt
PQDIASMVILGLTCL MMU97537 21 -1 -
PPVILSMVILSLTFL HSU62027 21 -1 -
PQDIASMVILGLTCL C3AR_MOUSE 21 -1 -
PPVILSMVILSLTFL C3AR_HUMAN 21 -1 -
PQDIASMVILSLTCL JC5835 21 -1 -

Motif 3 width=12
Element Seqn Id St Int Rpt
GLKMQRTVNTIW C3AR_HUMAN 49 13 -
GLKMQRTVNTIW HSU62027 49 13 -
GVKMKRTVNTVW JC5835 49 13 -
GVKMKTTVNTVW MMU97537 49 13 -
GVKMKTTVNTVW C3AR_MOUSE 49 13 -

Motif 4 width=13
Element Seqn Id St Int Rpt
SLPFSLAHLILQG MMU97537 74 13 -
SLPFSVAHLILRG JC5835 74 13 -
SLPFSLAHLALQG C3AR_HUMAN 74 13 -
SLPFSLAHLALQG HSU62027 74 13 -
SLPFSLAHLILQG C3AR_MOUSE 74 13 -

Motif 5 width=26
Element Seqn Id St Int Rpt
DRSAPSSVQARDYFWTVTTALQSQPF C3AR_MOUSE 212 125 -
DRSAPSSVQARDYFWTVTTALQSQPF MMU97537 212 125 -
DRTAPSSSVPARDLWTATTALQSQTF JC5835 208 121 -
DRLDPSSFQTNDHPWTVPTVFQPQTF HSU62027 209 122 -
DRLDPSSFQTNDHPWTVPTVFQPQTF C3AR_HUMAN 209 122 -

Motif 6 width=19
Element Seqn Id St Int Rpt
QFTYDNHVPTPLMAITITR C3AR_MOUSE 315 77 -
QLMYGNHAWTPQVAITISR JC5835 311 77 -
QFTYDNHVPTPLMAITITR MMU97537 315 77 -
QFTDDDQVPTPLVAITITR HSU62027 322 87 -
QFTDDDQVPTPLVAITITR C3AR_HUMAN 322 87 -

Motif 7 width=17
Element Seqn Id St Int Rpt
FRMRKTNFTKSRNKTFR C3AR_MOUSE 354 20 -
FRMQRGRFAKSQSKTFR HSU62027 361 20 -
FRMRKTNLTKSRNKTLR JC5835 350 20 -
FRMRKTNFTKSRNKTFR MMU97537 354 20 -
FRMQRGRFAKSQSKTFR C3AR_HUMAN 361 20 -

Motif 8 width=17
Element Seqn Id St Int Rpt
HIFGVLSLLTDPETPLG C3AR_HUMAN 394 16 -
HLVGILLLITDPESSLG MMU97537 386 15 -
HIVGILLVITDQESALR JC5835 383 16 -
HIFGVLSLLTDPETPLG HSU62027 394 16 -
HLVGVLLLITDPESSLG C3AR_MOUSE 387 16 -

Motif 9 width=14
Element Seqn Id St Int Rpt
MSWDHMSIALASAN MMU97537 406 3 -
MSWDHVCIALASAN C3AR_HUMAN 414 3 -
MSWDHMSIALASAN C3AR_MOUSE 407 3 -
LPWDHMSIALASAN JC5835 403 3 -
MSWDHVCIALASAN HSU62027 414 3 -

Motif 10 width=20
Element Seqn Id St Int Rpt
GKDFRKKARQSIKGILEAAF MMU97537 431 11 -
GKDFRKKARQSIQGILEAAF HSU62027 439 11 -
GKDFRKKARQSIKGILEAAF C3AR_MOUSE 432 11 -
GKDFRKKARQSIQGILEAAF C3AR_HUMAN 439 11 -
GKDFRKKARQSVKGILEAAF JC5835 428 11 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
SFSAETNSTDLLSQPWNEP C3AR_HUMAN 3 3 -
SFSAETNSTDLLSQPWNEP O43771 3 3 -
SFDADTNSTDLHSRPLFQP C3AR_MOUSE 3 3 -
SFTADTNSTDLHSRPLFKP O55197 3 3 -
SSSAETNSTGLHLEPQYQP O88680 3 3 -

Motif 2 width=15
Element Seqn Id St Int Rpt
PPVILSMVILSLTFL C3AR_HUMAN 21 -1 -
PPVILSMVILSLTFL O43771 21 -1 -
PQDIASMVILGLTCL C3AR_MOUSE 21 -1 -
PQDIASMVILSLTCL O55197 21 -1 -
PETILAMAILGLTFV O88680 21 -1 -

Motif 3 width=12
Element Seqn Id St Int Rpt
GLKMQRTVNTIW C3AR_HUMAN 49 13 -
GLKMQRTVNTIW O43771 49 13 -
GVKMKTTVNTVW C3AR_MOUSE 49 13 -
GVKMKRTVNTVW O55197 49 13 -
GLKMRRTVNTVW O88680 49 13 -

Motif 4 width=13
Element Seqn Id St Int Rpt
SLPFSLAHLALQG C3AR_HUMAN 74 13 -
SLPFSLAHLALQG O43771 74 13 -
SLPFSLAHLILQG C3AR_MOUSE 74 13 -
SLPFSVAHLILRG O55197 74 13 -
SLPFSMAHLALRG O88680 74 13 -

Motif 5 width=26
Element Seqn Id St Int Rpt
DRLDPSSFQTNDHPWTVPTVFQPQTF C3AR_HUMAN 209 122 -
DRLDPSSFQTNDHPWTVPTVFQPQTF O43771 209 122 -
DRSAPSSVQARDYFWTVTTALQSQPF C3AR_MOUSE 212 125 -
DRTAPSSSVPARDLWTATTALQSQTF O55197 208 121 -
HRSDPSSFQTQDGPWSVTTTLYSQTS O88680 210 123 -

Motif 6 width=19
Element Seqn Id St Int Rpt
QFTDDDQVPTPLVAITITR C3AR_HUMAN 322 87 -
QFTDDDQVPTPLVAITITR O43771 322 87 -
QFTYDNHVPTPLMAITITR C3AR_MOUSE 315 77 -
QLMYGNHAWTPQVAITISR O55197 311 77 -
PFTHEYRTPRLLKVITFTR O88680 313 77 -

Motif 7 width=17
Element Seqn Id St Int Rpt
FRMQRGRFAKSQSKTFR C3AR_HUMAN 361 20 -
FRMQRGRFAKSQSKTFR O43771 361 20 -
FRMRKTNFTKSRNKTFR C3AR_MOUSE 354 20 -
FRMRKTNLTKSRNKTLR O55197 350 20 -
FRMRRVRVVKSWNKALH O88680 352 20 -

Motif 8 width=17
Element Seqn Id St Int Rpt
HIFGVLSLLTDPETPLG C3AR_HUMAN 394 16 -
HIFGVLSLLTDPETPLG O43771 394 16 -
HLVGVLLLITDPESSLG C3AR_MOUSE 387 16 -
HIVGILLVITDQESALR O55197 383 16 -
HVFGVLILFINPESRVG O88680 385 16 -

Motif 9 width=14
Element Seqn Id St Int Rpt
MSWDHVCIALASAN C3AR_HUMAN 414 3 -
MSWDHVCIALASAN O43771 414 3 -
MSWDHMSIALASAN C3AR_MOUSE 407 3 -
LPWDHMSIALASAN O55197 403 3 -
LSWDHVSIALASAN O88680 405 3 -

Motif 10 width=20
Element Seqn Id St Int Rpt
GKDFRKKARQSIQGILEAAF C3AR_HUMAN 439 11 -
GKDFRKKARQSIQGILEAAF O43771 439 11 -
GKDFRKKARQSIKGILEAAF C3AR_MOUSE 432 11 -
GKDFRKKARQSVKGILEAAF O55197 428 11 -
GRDLRKRVRQSMKGILEAAF O88680 430 11 -