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PR01049

Identifier
PHOSPHBKNASE  [View Relations]  [View Alignment]  
Accession
PR01049
No. of Motifs
7
Creation Date
22-DEC-1998  (UPDATE 07-JUN-1999)
Title
Phosphorylase kinase family signature
Database References

PROSITE; PS50011 PROTEIN_KINASE_DOM; PS00108 PROTEIN_KINASE_ST; PS00107 PROTEIN_KINASE_ATP

INTERPRO; IPR002291
PDB; 1PHK
SCOP; 1PHK
CATH; 1PHK
Literature References
1. YU, J.S. AND YANG, S.D.
Phosphorylation activation of phosphorylase-b kinase by cAMP,
Ca2+-independent, autophosphorylation-dependent protein-kinase.
BIOCHEM.BIOPHYS.RES.COMMUN. 207 140-147 (1995).
 
2. POLISHCHUK, S.V., BRANDT, N.R., MEYER, H.E., VARSANYI, M. AND 
HEILMEYER, L.M.G.
Does phosphorylase-kinase control glycogen biosynthesis in skeletal muscle.
FEBS LETT. 362 271-275 (1995).
 
3.ZEMSKOVA, M.A., SHUR, S.A., SKOLYSHEVA, L.K. AND VULFSON, P.L.
Association of rabbit skeletal muscle phosphorylase kinase with sarcoplasmic
reticulum membranes.
BIOCHEMISTRY 60 1457-1463 (1995).
 
4. YU, J.S., LEE, S.C. AND YANG, S.D.
Effect of Mg2+ concentrations on phosphorylation activation of phosphorylase
b kinase by cAMP/Ca2+ independent, autophosphorylation-dependent protein
kinase.
J.PROTEIN CHEM. 14 747-752 (1995).
 
5. KURGANOV, B.I., ANDREEVA, I.E., MAKEEVA, V.F. AND LIVANOVA, N.B.
Hystereticproperties of rabbit skeletal muscle phosphorylase kinase:
Synergistic activation by phosphorylase b, Ca2+, and Mg2+.
BIOCHEM.MOL.BIOL.INT. 39 805-812 (1996).
 
6. LAFORET, P., EYMARD, B., LOMBES, A., DUBOC, D., JEHENSON, P.,
ROCCHICCIOLI, F., CHAUSSAIN, M., CHATEAU, D., BRUNET, P. AND FARDEAU, M.
Exercise intolerance due to muscular phosphorylase kinase deficiency.
Contribution of in vivo metabolism investigations.
REV.NEUROL. 152 458-464 (1996).
 
7. SAHIN, G., GUNGOR, T., RETTWITZVOLK, W., SCHOLTE, W., SHIN, Y.S.,
PODSKARBI, T. AND SEWELL, A.C.
Infantile muscle phosphorylase-b-kinase deficiency. A case report.
NEUROPEDIATRICS 29 48-50 (1998).
 
8. KAGALWALLA, A.F., KAGALWALLA, Y.A., ALAJAJI, S., GORKA, W. AND ASHRAF, M.
Phosphorylase-b kinase deficiency glycogenosis with cirrhosis of the liver.
J.PEDIATRICS 127 602-605 (1995).

Documentation
Phosphorylase b kinase (EC 2.7.1.38) belongs to a family of proteins 
involved in glycogen biosynthesis [1]. The protein has a subunit composition
of (alpha, beta, gamma, delta)4, where the alpha and beta subunits are 
regulatory, delta is calmodulin, and the gamma subunit is catalytic. The 
enzyme is believed to have a dual role: the first is connected with glycogen
degradation via phosphorylation of glycogen phosphorylase; the second 
controls glycogen biosynthesis on the sarcoplasmic reticular membrane more
directly by phosphorylation, and thus inhibition, of glycogen synthase [2].
 
The gamma catalytic chain contains three domains; one protein kinase and two
calmodulin-binding domains. Calcium and magnesium ions, together with cyclic
AMP, positively affect the efficiency of the enzyme, which is believed to 
be associated with its auto-kinase activity [1,3-5].
 
The full extent of the effects of deficiencies in this enzyme in humans is 
unknown; but case studies have been documented [6-8] that detail symptoms as
mild as `exercise intolerance' [6], to infant mortality arising from floppy
infant syndrome [7].
 
PHOSPHBKNASE is a 7-element fingerprint that provides a signature for the
phosphorylase kinase family. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions
spanning the full alignment length - in the kinase domain, motif 1 spans 
the C-terminus of helix 2 and the N-terminus of helix 3; motif 2 spans 
helices 12 and 13; and the remaining motifs reside in the calmodulin-binding
domains. Two iterations on OWL30.2 were required to reach convergence, at
which point a true set comprising 8 sequences was identified.
 
An update on SPTR37_9f identified a true set of 7 sequences.
Summary Information
7 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
77777777
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
KPBG_HUMAN    KPBG_MOUSE    KPBG_RABIT    KPBG_RAT      
KPBH_HUMAN KPBH_RAT Q23996
Sequence Titles
KPBG_HUMAN  PHOSPHORYLASE B KINASE GAMMA CATALYTIC CHAIN, SKELETAL MUSCLE ISOFORM (EC 2.7.1.38) (PHOSPHORYLASE KINASE GAMMA SUBUNIT) - HOMO SAPIENS (HUMAN). 
KPBG_MOUSE PHOSPHORYLASE B KINASE GAMMA CATALYTIC CHAIN, SKELETAL MUSCLE ISOFORM (EC 2.7.1.38) (PHOSPHORYLASE KINASE GAMMA SUBUNIT) - MUS MUSCULUS (MOUSE).
KPBG_RABIT PHOSPHORYLASE B KINASE GAMMA CATALYTIC CHAIN, SKELETAL MUSCLE ISOFORM (EC 2.7.1.38) (PHOSPHORYLASE KINASE GAMMA SUBUNIT) - ORYCTOLAGUS CUNICULUS (RABBIT).
KPBG_RAT PHOSPHORYLASE B KINASE GAMMA CATALYTIC CHAIN, SKELETAL MUSCLE ISOFORM (EC 2.7.1.38) (PHOSPHORYLASE KINASE GAMMA SUBUNIT) - RATTUS NORVEGICUS (RAT).
KPBH_HUMAN PHOSPHORYLASE B KINASE GAMMA CATALYTIC CHAIN, TESTIS ISOFORM (EC 2.7.1.38) (PHK-GAMMA-T) (PSK-C3) (PHOSPHORYLASE KINASE GAMMA SUBUNIT) - HOMO SAPIENS (HUMAN).
KPBH_RAT PHOSPHORYLASE B KINASE GAMMA CATALYTIC CHAIN, TESTIS ISOFORM (EC 2.7.1.38) (PHK-GAMMA-T) (PHOSPHORYLASE KINASE GAMMA SUBUNIT) - RATTUS NORVEGICUS (RAT).
Q23996 PHOSPHORYLASE KINASE GAMMA - DROSOPHILA MELANOGASTER (FRUIT FLY).
Scan History
OWL30_2    2  50   NSINGLE    
SPTR37_9f 2 8 NSINGLE
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
TEKVTLSEKETRKIMRALLEVI KPBG_RABIT 116 116 -
TEKVTLSEKETRKIMRALLEVI KPBG_HUMAN 116 116 -
TEKVTLTEKETRKIMRALLEVV KPBG_RAT 116 116 -
TEKVTLTEKETRKIMRALLEVI KPBG_MOUSE 116 116 -
TEKVALSEKETRSIMRSLLEAV KPBH_RAT 120 120 -
TEKVALSEKETRSIMRSLLEAV KPBH_HUMAN 120 120 -
TSVVTLSEKKTRTIMRQIFEGV S51600 120 120 -

Motif 2 width=12
Element Seqn Id St Int Rpt
EEALAHPFFQQY KPBG_RABIT 279 141 -
EEALAHPFFQEY KPBG_RAT 279 141 -
KEVLRHPFFNQM S51600 283 141 -
EQALQHPFFERC KPBH_HUMAN 283 141 -
EQALQHPFFERC KPBH_RAT 283 141 -
EEALAHPFFQEY KPBG_MOUSE 279 141 -
EEALAHPFFQQY KPBG_HUMAN 279 141 -

Motif 3 width=16
Element Seqn Id St Int Rpt
WNLTPRQRFRVAVWTV KPBH_HUMAN 300 5 -
SRFNARKKFQFAILVI S51600 468 173 -
RHFSPRGKFKVICLTV KPBG_RABIT 296 5 -
RHFSPRGKFKVIALTV KPBG_HUMAN 296 5 -
RHFSPRGKFKVICLTV KPBG_RAT 296 5 -
RHFSPRGKFKVICLTV KPBG_MOUSE 296 5 -
WNLTPRQRFRVAVWTI KPBH_RAT 300 5 -

Motif 4 width=19
Element Seqn Id St Int Rpt
LASVKIYYQYRRVKPVTRE KPBG_MOUSE 312 0 -
LAAGRVALSSHRLRPLTKN KPBH_RAT 316 0 -
LAAGRVALSTHRVRPLTKN KPBH_HUMAN 316 0 -
RAIIRIRRLRFTAEPLHVE S51600 484 0 -
LASVRIYYQYRRVKPVTRE KPBG_RABIT 312 0 -
LASVRIYYQYRRVKPVTRE KPBG_HUMAN 312 0 -
LASVRIYYQYRRVKPVTRE KPBG_RAT 312 0 -

Motif 5 width=15
Element Seqn Id St Int Rpt
IVIRDPYALRPLRRL KPBG_MOUSE 331 0 -
ALLRDPYALRSVRHL KPBH_HUMAN 335 0 -
EAIRDPYRVKVMRKV S51600 503 0 -
IVIRDPYALRPLRRL KPBG_RABIT 331 0 -
IVIRDPYALRPLRRL KPBG_HUMAN 331 0 -
IVIRDPYALRPLRRL KPBG_RAT 331 0 -
ALLRDPYALRPVRRL KPBH_RAT 335 0 -

Motif 6 width=16
Element Seqn Id St Int Rpt
IDNCAFRLYGHWVKKG KPBH_RAT 350 0 -
IDGCACRVYGHWVKKG S51600 518 0 -
IDAYAFRIYGHWVKKG KPBG_RABIT 346 0 -
IDAYAFRIYGHWVKKG KPBG_HUMAN 346 0 -
IDAYAFRIYGHWVKKG KPBG_RAT 346 0 -
IDAYAFRIYGHWVKKG KPBG_MOUSE 346 0 -
IDNCAFRLYGHWVKKG KPBH_HUMAN 350 0 -

Motif 7 width=14
Element Seqn Id St Int Rpt
QQQNRAALFENTPK KPBG_RAT 362 0 -
EQQNRAALFQHQPP KPBH_RAT 366 0 -
EQQNRAALFQHRPP KPBH_HUMAN 366 0 -
QQQNRAALFENTPK KPBG_RABIT 362 0 -
EGQDRAALFENTPR S51600 534 0 -
QQQNRAALFENTPK KPBG_HUMAN 362 0 -
QQQNRAALFENTPK KPBG_MOUSE 362 0 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
TEKVTLSEKETRKIMRALLEVI KPBG_RABIT 116 116 -
TEKVTLSEKETRKIMRALLEVI KPBG_HUMAN 116 116 -
TEKVTLTEKETRKIMRALLEVV KPBG_RAT 116 116 -
TEKVTLTEKETRKIMRALLEVI KPBG_MOUSE 116 116 -
TEKVALSEKETRSIMRSLLEAV KPBH_RAT 120 120 -
TEKVALSEKETRSIMRSLLEAV KPBH_HUMAN 120 120 -
TSVVTLSEKKTRTIMRQIFEGV Q23996 120 120 -

Motif 2 width=12
Element Seqn Id St Int Rpt
EEALAHPFFQQY KPBG_RABIT 279 141 -
EEALAHPFFQQY KPBG_HUMAN 279 141 -
EEALAHPFFQEY KPBG_RAT 279 141 -
EEALAHPFFQEY KPBG_MOUSE 279 141 -
EQALQHPFFERC KPBH_RAT 283 141 -
EQALQHPFFERC KPBH_HUMAN 283 141 -
KEVLRHPFFNQM Q23996 283 141 -

Motif 3 width=16
Element Seqn Id St Int Rpt
RHFSPRGKFKVICLTV KPBG_RABIT 296 5 -
RHFSPRGKFKVIALTV KPBG_HUMAN 296 5 -
RHFSPRGKFKVICLTV KPBG_RAT 296 5 -
RHFSPRGKFKVICLTV KPBG_MOUSE 296 5 -
WNLTPRQRFRVAVWTI KPBH_RAT 300 5 -
WNLTPRQRFRVAVWTV KPBH_HUMAN 300 5 -
SRFNARKKFQFAILVI Q23996 468 173 -

Motif 4 width=19
Element Seqn Id St Int Rpt
LASVRIYYQYRRVKPVTRE KPBG_RABIT 312 0 -
LASVRIYYQYRRVKPVTRE KPBG_HUMAN 312 0 -
LASVRIYYQYRRVKPVTRE KPBG_RAT 312 0 -
LASVKIYYQYRRVKPVTRE KPBG_MOUSE 312 0 -
LAAGRVALSSHRLRPLTKN KPBH_RAT 316 0 -
LAAGRVALSTHRVRPLTKN KPBH_HUMAN 316 0 -
RAIIRIRRLRFTAEPLHVE Q23996 484 0 -

Motif 5 width=15
Element Seqn Id St Int Rpt
IVIRDPYALRPLRRL KPBG_RABIT 331 0 -
IVIRDPYALRPLRRL KPBG_HUMAN 331 0 -
IVIRDPYALRPLRRL KPBG_RAT 331 0 -
IVIRDPYALRPLRRL KPBG_MOUSE 331 0 -
ALLRDPYALRPVRRL KPBH_RAT 335 0 -
ALLRDPYALRSVRHL KPBH_HUMAN 335 0 -
EAIRDPYRVKVMRKV Q23996 503 0 -

Motif 6 width=16
Element Seqn Id St Int Rpt
IDAYAFRIYGHWVKKG KPBG_RABIT 346 0 -
IDAYAFRIYGHWVKKG KPBG_HUMAN 346 0 -
IDAYAFRIYGHWVKKG KPBG_RAT 346 0 -
IDAYAFRIYGHWVKKG KPBG_MOUSE 346 0 -
IDNCAFRLYGHWVKKG KPBH_RAT 350 0 -
IDNCAFRLYGHWVKKG KPBH_HUMAN 350 0 -
IDGCACRVYGHWVKKG Q23996 518 0 -

Motif 7 width=14
Element Seqn Id St Int Rpt
QQQNRAALFENTPK KPBG_RABIT 362 0 -
QQQNRAALFENTPK KPBG_HUMAN 362 0 -
QQQNRAALFENTPK KPBG_RAT 362 0 -
QQQNRAALFENTPK KPBG_MOUSE 362 0 -
EQQNRAALFQHQPP KPBH_RAT 366 0 -
EQQNRAALFQHRPP KPBH_HUMAN 366 0 -
EGQDRAALFENTPR Q23996 534 0 -