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PR01017

Identifier
NRPEPTIDEY6R  [View Relations]  [View Alignment]  
Accession
PR01017
No. of Motifs
6
Creation Date
07-DEC-1998  (UPDATE 03-JUL-1999)
Title
Neuropeptide Y6 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01012 NRPEPTIDEYR
INTERPRO; IPR000986
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Neuropeptide Y.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.194-198.
 
7. WEINBERG, D.H., SIRINATHSINGHJI, D.J., TAN, C.P., SHIAO, L.L., MORIN, N.,
RIGBY, M.R., HEAVENS, R.H., RAPOPORT, D.R., BAYNE, M.L., CASCIERI, M.A.,
STRADER, C.D., LINEMEYER, D.L. AND MACNEIL, D.J.
Cloning and expression of a novel neuropeptide Y receptor.
J.BIOL.CHEM. 271 16435-16438 (1996). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Neuropeptide Y (NPY) is one of the most abundant peptides in mammalian
brain, inducing a variety of behavioural effects (e.g., stimulation of food
intake, anxiety, facilitation of learning and memory, and regulation of the
cardiovascular and neuroendocrine systems) [6]. In the periphery, NPY 
stimulates vascular smooth muscle contraction and modulates hormone
secretion. NPY has been implicated in the pathophysiology of hypertension, 
congestive heart failure, affective disorders and appetite regulation [6].
 
Several pharmacologically distinct neuropeptide Y receptors have been 
characterised, designated NPY Y1-Y6. NPY Y6 shares 60% sequence identity 
with the Y1 receptor. Its pharmacology resembles that of the Y1 receptor 
and is distinct from that described for Y2, Y3 and Y4 receptors [7]. The
receptor is expressed within discrete regions of the hypothalamus, including
the suprachiasmatic nucleus, anterior hypothalamus, bed nucleus stria 
terminalis, and the ventromedial nucleus, with no localisation apparent
elsewhere in the brain [7]. 
 
NRPEPTIDEY6R is a 6-element fingerprint that provides a signature for
neuropeptide Y6 receptors. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the Y6 receptors but distinguish them from the rest of
the neuropeptide Y family - motifs 1 and 2 span the N-terminus, leading
into TM domain 1; motifs 3 and 4 span the C-terminus of TM domain 4, leading
into the second external loop; motif 5 resides in the third cytoplasmic 
loop; and motif 6 lies at the C-terminus. A single iteration on OWL30.2 was
required to reach convergence, no further sequences being identified beyond
the starting set. Four partial matches were found: D86519 and HSU59431 are
neuropeptide Y6 receptor fragments that lack the portion of sequence bearing
the final motif; and AF005779 and OAU62122 are neuropeptide Y1 receptors 
that match motifs 3 and 5. 
 
An update on SPTR37_9f identified a true set of 2 sequences, and 3
partial matches.
Summary Information
   2 codes involving  6 elements
2 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
6222222
5222220
4000000
3000000
2001001
123456
True Positives
NY6R_MOUSE    NY6R_RABIT    
True Positive Partials
Codes involving 5 elements
Q99463 Q99647
Codes involving 2 elements
O57463
Sequence Titles
NY6R_MOUSE  NEUROPEPTIDE Y RECEPTOR TYPE 6 (NPY6-R) (PANCREATIC POLYPEPTIDE RECEPTOR 2) (PP2) - MUS MUSCULUS (MOUSE). 
NY6R_RABIT NEUROPEPTIDE Y RECEPTOR TYPE 6 (NPY6-R) - ORYCTOLAGUS CUNICULUS (RABBIT).

Q99463 Y6 ENCODING PROTEIN - HOMO SAPIENS (HUMAN).
Q99647 TRUNCATED PANCREATIC POLYPEPTIDE RECEPTOR PP2 - HOMO SAPIENS (HUMAN).

O57463 NEUROPEPTIDE Y /PEPTIDE YY RECEPTOR YB - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
Scan History
OWL30_2    1  100  NSINGLE    
SPTR37_9f 1 75 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
EVSLNDPASNKTSAKSNSS D86521 2 2 -
EVLTNQPTPNKTSGKSNNS NY6R_MOUSE 2 2 -

Motif 2 width=16
Element Seqn Id St Int Rpt
AFFYFESCQSPSLALL D86521 21 0 -
AFFYFESCQPPFLAIL NY6R_MOUSE 21 0 -

Motif 3 width=15
Element Seqn Id St Int Rpt
LSYHLTDEPFRNLSL D86521 171 134 -
LSYHLTNEPFHNLSL NY6R_MOUSE 171 134 -

Motif 4 width=14
Element Seqn Id St Int Rpt
LPTDLYSHHVVCVE D86521 185 -1 -
LPTDIYTHQVACVE NY6R_MOUSE 185 -1 -

Motif 5 width=15
Element Seqn Id St Int Rpt
LKIVICLHKRNSKID D86521 230 31 -
LKIVLCLRKRTRQVD NY6R_MOUSE 230 31 -

Motif 6 width=19
Element Seqn Id St Int Rpt
LVVLIHHCLCFALRERYEN D86521 329 84 -
LMMLIHHCWCGEPQESYEN NY6R_MOUSE 329 84 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
EVLTNQPTPNKTSGKSNNS NY6R_MOUSE 2 2 -
EVSLNDPASNKTSAKSNSS NY6R_RABIT 2 2 -

Motif 2 width=16
Element Seqn Id St Int Rpt
AFFYFESCQPPFLAIL NY6R_MOUSE 21 0 -
AFFYFESCQSPSLALL NY6R_RABIT 21 0 -

Motif 3 width=15
Element Seqn Id St Int Rpt
LSYHLTNEPFHNLSL NY6R_MOUSE 171 134 -
LSYHLTDEPFRNLSL NY6R_RABIT 171 134 -

Motif 4 width=14
Element Seqn Id St Int Rpt
LPTDIYTHQVACVE NY6R_MOUSE 185 -1 -
LPTDLYSHHVVCVE NY6R_RABIT 185 -1 -

Motif 5 width=15
Element Seqn Id St Int Rpt
LKIVLCLRKRTRQVD NY6R_MOUSE 230 31 -
LKIVICLHKRNSKID NY6R_RABIT 230 31 -

Motif 6 width=19
Element Seqn Id St Int Rpt
LMMLIHHCWCGEPQESYEN NY6R_MOUSE 329 84 -
LVVLIHHCLCFALRERYEN NY6R_RABIT 329 84 -