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PR01016

Identifier
NRPEPTIDEY5R  [View Relations]  [View Alignment]  
Accession
PR01016
No. of Motifs
12
Creation Date
30-NOV-1998  (UPDATE 07-JUN-1999)
Title
Neuropeptide Y5 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01012 NRPEPTIDEYR
INTERPRO; IPR000393
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Neuropeptide Y.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.194-198.
 
7. GERALD, C., WALKER, M.W., CRISCIONE, L., GUSTAFSON, E.L., BATZL-HARTMANN, 
C., SMITH, K.E., VAYSSE, P., DURKIN, M.M., LAZ, T.M., LINEMEYER, D.L., 
SCHAFFHAUSER, A.O., WHITEBREAD, S., HOFBAUER, K.G., TABER, R.I., BRANCHEK,
T.A. AND WEINSHANK, R.L.
A receptor subtype involved in neuropeptide-Y-induced food intake.
NATURE 382 168-171 (1996). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Neuropeptide Y (NPY) is one of the most abundant peptides in mammalian
brain, inducing a variety of behavioural effects (e.g., stimulation of food
intake, anxiety, facilitation of learning and memory, and regulation of the
cardiovascular and neuroendocrine systems) [6]. In the periphery, NPY 
stimulates vascular smooth muscle contraction and modulates hormone
secretion. NPY has been implicated in the pathophysiology of hypertension, 
congestive heart failure, affective disorders and appetite regulation [6].
 
Several pharmacologically distinct neuropeptide Y receptors have been 
characterised, designated NPY Y1-Y6. The Y5 receptor is a 456-residue
protein with less than 35% overall identity to known Y-type receptors [7].
The messenger RNA is found primarily in the central nervous system,
including the paraventricular nucleus of the hypothalamus [7]. Y5 receptors
have been postulated to be the `feeding' receptors, and may provide new 
approaches for the study and treatment of obesity and eating disorders [7]. 
 
NRPEPTIDEY5R is a 12-element fingerprint that provides a signature for
neuropeptide Y5 receptors. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the Y5 receptors but distinguish them from the rest of
the neuropeptide Y family - motifs 1 and 2 reside at the N-terminus,
leading into TM domain 1; motifs 3 and 4 span the C-terminus of TM domain
3, the second cytoplasmic loop and the N-terminal portion of TM domain 4;
motifs 5 and 6 span the C-terminal portion of TM domain 4 and the second
external loop; motifs 7-10 span the third cytoplasmic loop; motif 11 spans
the third external loop; and motif 12 resides at the C-terminus. Two 
iterations on OWL30.2 were required to reach convergence, at which point
a true set comprising 3 sequences was identified. 
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving 12 elements
0 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
12555555555555
11000000000000
10000000000000
9000000000000
8000000000000
7000000000000
6000000000000
5000000000000
4000000000000
3000000000000
2000000000000
123456789101112
True Positives
NY5R_CANFA    NY5R_HUMAN    NY5R_MOUSE    NY5R_RAT      
O35380
Sequence Titles
NY5R_CANFA  NEUROPEPTIDE Y RECEPTOR TYPE 5 (NPY5-R) (Y5 RECEPTOR) (NPYY5) - CANIS FAMILIARIS (DOG). 
NY5R_HUMAN NEUROPEPTIDE Y RECEPTOR TYPE 5 (NPY5-R) (Y5 RECEPTOR) (NPYY5) - HOMO SAPIENS (HUMAN).
NY5R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 5 (NPY5-R) (Y5 RECEPTOR) - MUS MUSCULUS (MOUSE).
NY5R_RAT NEUROPEPTIDE Y RECEPTOR TYPE 5 (NPY5-R) (Y5 RECEPTOR) - RATTUS NORVEGICUS (RAT).
O35380 NEUROPEPTIDE Y Y5 RECEPTOR - MUS MUSCULUS (MOUSE).
Scan History
OWL30_2    2  100  NSINGLE    
SPTR37_9f 2 8 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
LEEHFNKTFVTENNT NY5R_RAT 16 16 -
LDEYYNKTLATENNT NY5R_HUMAN 15 15 -

Motif 2 width=15
Element Seqn Id St Int Rpt
YRGSVDDLQYFLIGL NY5R_RAT 44 13 -
YKSSVDDLQYFLIGL NY5R_HUMAN 43 13 -

Motif 3 width=14
Element Seqn Id St Int Rpt
VRYHMIKHPISNNL NY5R_RAT 149 90 -
VRYHMIKHPISNNL NY5R_HUMAN 148 90 -

Motif 4 width=12
Element Seqn Id St Int Rpt
TANHGYFLIATV NY5R_RAT 163 0 -
TANHGYFLIATV NY5R_HUMAN 162 0 -

Motif 5 width=17
Element Seqn Id St Int Rpt
GFAICSPLPVFHSLVEL NY5R_RAT 178 3 -
GFAICSPLPVFHSLVEL NY5R_HUMAN 177 3 -

Motif 6 width=17
Element Seqn Id St Int Rpt
ETFGSALLSSKYLCVES NY5R_RAT 196 1 -
ETFGSALLSSRYLCVES NY5R_HUMAN 195 1 -

Motif 7 width=20
Element Seqn Id St Int Rpt
ENRLEENEMINLTLQPSKKS NY5R_RAT 257 44 -
ENRLEENEMINLTLHPSKKS NY5R_HUMAN 256 44 -

Motif 8 width=25
Element Seqn Id St Int Rpt
KWSYSFIRKHRRRYSKKTACVLPAP NY5R_RAT 287 10 -
KWSYSFIKKHRRRYSKKTACVLPAP NY5R_HUMAN 286 10 -

Motif 9 width=18
Element Seqn Id St Int Rpt
KVIPGVPICFEVKPEESS NY5R_RAT 338 26 -
KFIPGVPTCFEIKPEENS NY5R_HUMAN 338 27 -

Motif 10 width=24
Element Seqn Id St Int Rpt
DAHEMRVKRSITRIKKRSRSVFYR NY5R_RAT 356 0 -
DVHELRVKRSVTRIKKRSRSVFYR NY5R_HUMAN 356 0 -

Motif 11 width=17
Element Seqn Id St Int Rpt
HVVTDFNDNLISNRHFK NY5R_RAT 398 18 -
HVVTDFNDNLISNRHFK NY5R_HUMAN 398 18 -

Motif 12 width=17
Element Seqn Id St Int Rpt
LNNGIKADLRALIHCLH NY5R_RAT 438 23 -
LNNGIKADLVSLIHCLH NY5R_HUMAN 438 23 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
LEEHFNKTFVTENNT O35380 5 5 -
LEEHFNKTFVTENNT NY5R_RAT 16 16 -
LEEHFNKTFVTENNT NY5R_MOUSE 5 5 -
LDEYYNKTLATENNT NY5R_HUMAN 15 15 -
LQDFYNKTLATENNT NY5R_CANFA 5 5 -

Motif 2 width=15
Element Seqn Id St Int Rpt
YRGSVDDLQYFLIGL O35380 54 34 -
YRGSVDDLQYFLIGL NY5R_RAT 44 13 -
YRGSVDDLQYFLIGL NY5R_MOUSE 54 34 -
YKSSVDDLQYFLIGL NY5R_HUMAN 43 13 -
YKSSVDDLQYFLIGL NY5R_CANFA 33 13 -

Motif 3 width=14
Element Seqn Id St Int Rpt
VRYHMIKHPISNNL O35380 159 90 -
VRYHMIKHPISNNL NY5R_RAT 149 90 -
VRYHMIKHPISNNL NY5R_MOUSE 159 90 -
VRYHMIKHPISNNL NY5R_HUMAN 148 90 -
VRYHMIKHPISNNL NY5R_CANFA 138 90 -

Motif 4 width=12
Element Seqn Id St Int Rpt
TANHGYFLIATV O35380 173 0 -
TANHGYFLIATV NY5R_RAT 163 0 -
TANHGYFLIATV NY5R_MOUSE 173 0 -
TANHGYFLIATV NY5R_HUMAN 162 0 -
TANHGYFLIATV NY5R_CANFA 152 0 -

Motif 5 width=17
Element Seqn Id St Int Rpt
GFAICSPLPVFHSLVEL O35380 188 3 -
GFAICSPLPVFHSLVEL NY5R_RAT 178 3 -
GFAICSPFPVFHSLVEL NY5R_MOUSE 188 3 -
GFAICSPLPVFHSLVEL NY5R_HUMAN 177 3 -
GFAICSPLPVFHSLVEL NY5R_CANFA 167 3 -

Motif 6 width=17
Element Seqn Id St Int Rpt
ETFGSALLSSKYLCVES O35380 206 1 -
ETFGSALLSSKYLCVES NY5R_RAT 196 1 -
ETFGSALLSSKYLCVES NY5R_MOUSE 206 1 -
ETFGSALLSSRYLCVES NY5R_HUMAN 195 1 -
ETFDSALLSSRYLCVES NY5R_CANFA 185 1 -

Motif 7 width=20
Element Seqn Id St Int Rpt
ENRLEENEMINLTLHPSKKS O35380 267 44 -
ENRLEENEMINLTLQPSKKS NY5R_RAT 257 44 -
ENRLEENEMINLTLHPSKKS NY5R_MOUSE 267 44 -
ENRLEENEMINLTLHPSKKS NY5R_HUMAN 256 44 -
ENKLEENEMINLTLQPFKKS NY5R_CANFA 246 44 -

Motif 8 width=25
Element Seqn Id St Int Rpt
KWSYSFIRKHRRRYSKKTACVLPAP O35380 297 10 -
KWSYSFIRKHRRRYSKKTACVLPAP NY5R_RAT 287 10 -
KWSYSFIRKHRRRYSKKTACVLPAP NY5R_MOUSE 297 10 -
KWSYSFIKKHRRRYSKKTACVLPAP NY5R_HUMAN 286 10 -
KWSYSFIRKHRRRYSKKTACVLPAP NY5R_CANFA 276 10 -

Motif 9 width=18
Element Seqn Id St Int Rpt
KVIPGVPICFEVKPEESS O35380 348 26 -
KVIPGVPICFEVKPEESS NY5R_RAT 338 26 -
KVIPGVPICFEVKPEESS NY5R_MOUSE 348 26 -
KFIPGVPTCFEIKPEENS NY5R_HUMAN 338 27 -
KFIPGVPTCFEVKPEENS NY5R_CANFA 328 27 -

Motif 10 width=24
Element Seqn Id St Int Rpt
DAQEMRVKRSLTRIKKRSRSVFYR O35380 366 0 -
DAHEMRVKRSITRIKKRSRSVFYR NY5R_RAT 356 0 -
DAQEMRVKRSLTRIKKRSRSVFYR NY5R_MOUSE 366 0 -
DVHELRVKRSVTRIKKRSRSVFYR NY5R_HUMAN 356 0 -
DVHDMRVNRSIMRIKKRSRSVFYR NY5R_CANFA 346 0 -

Motif 11 width=17
Element Seqn Id St Int Rpt
HVVTDFNDNLISNRHFK O35380 408 18 -
HVVTDFNDNLISNRHFK NY5R_RAT 398 18 -
HVVTDFNDNLISNRHFK NY5R_MOUSE 408 18 -
HVVTDFNDNLISNRHFK NY5R_HUMAN 398 18 -
HVVTDFNDNLISNRHFK NY5R_CANFA 388 18 -

Motif 12 width=17
Element Seqn Id St Int Rpt
LNNGIKADLRALIHCLH O35380 448 23 -
LNNGIKADLRALIHCLH NY5R_RAT 438 23 -
LNNGIKADLRALIHCLH NY5R_MOUSE 448 23 -
LNNGIKADLVSLIHCLH NY5R_HUMAN 438 23 -
LNNGIKADLISLIQCLH NY5R_CANFA 428 23 -