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PR01015

Identifier
NRPEPTIDEY4R  [View Relations]  [View Alignment]  
Accession
PR01015
No. of Motifs
10
Creation Date
30-NOV-1998  (UPDATE 07-JUN-1999)
Title
Neuropeptide Y4 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01012 NRPEPTIDEYR
INTERPRO; IPR001933
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Neuropeptide Y.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.194-198.
 
7. BARD, J.A., WALKER, M.W., BRANCHEK, T.A. AND WEINSHANK, R.L.
Cloning and functional expression of a human Y4 subtype receptor for
pancreatic polypeptide, neuropeptide Y, and peptide YY.
J.BIOL.CHEM. 270 26762-26765 (1995). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Neuropeptide Y (NPY) is one of the most abundant peptides in mammalian
brain, inducing a variety of behavioural effects (e.g., stimulation of food
intake, anxiety, facilitation of learning and memory, and regulation of the
cardiovascular and neuroendocrine systems) [6]. In the periphery, NPY 
stimulates vascular smooth muscle contraction and modulates hormone
secretion. NPY has been implicated in the pathophysiology of hypertension, 
congestive heart failure, affective disorders and appetite regulation [6].
 
Several pharmacologically distinct neuropeptide Y receptors have been 
characterised, designated NPY Y1-Y6. Y4 receptors show highest similarity
to Y1 receptors from human, rat and mouse [7]. It is believed that they
may have potential roles in central nervous system, cardiovascular and
gastrointestinal function [7]. 
 
NRPEPTIDEY4R is a 10-element fingerprint that provides a signature for
neuropeptide Y4 receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the Y4 receptors but distinguish them from the rest of
the neuropeptide Y family - motifs 1-3 span the N-terminus, leading into 
TM domain 1; motifs 4 and 5 span the C-terminus of TM domain 4, leading
into second external loop; motif 6 lies in the third cytoplasmic loop; motif
7 lies in the third external loop; and motifs 8-10 span the C-terminus. A
single iteration on OWL30.2 was required to reach convergence, no further
sequences being identified beyond the starting set. A single partial match 
was found, AF030245, a zebrafish neuropeptide Y receptor that matches motifs
7 and 9.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
103333333333
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
NY4R_HUMAN    NY4R_MOUSE    NY4R_RAT      
Sequence Titles
NY4R_HUMAN  Neuropeptide Y receptor type 4 (NPY4-R) (Pancreatic polypeptide receptor 1) (PP1) - Homo sapiens (Human). 
NY4R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 4 (NPY4-R) (PANCREATIC POLYPEPTIDE RECEPTOR 1) (PP1) (NPYR-D) - MUS MUSCULUS (MOUSE).
NY4R_RAT NEUROPEPTIDE Y RECEPTOR TYPE 4 (NPY4-R) (PANCREATIC POLYPEPTIDE RECEPTOR 1) (PP1) - RATTUS NORVEGICUS (RAT).
Scan History
OWL30_2    1  50   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
NTSHFLAPLFPGSLQG NY4R_MOUSE 2 2 -
NTSHLMASLSPAFLQG NY4R_RAT 2 2 -
NTSHLLALLLPKSPQG NY4R_HUMAN 2 2 -

Motif 2 width=14
Element Seqn Id St Int Rpt
NGTNPLDSPYNFSD NY4R_MOUSE 19 1 -
NGTNPLDSLYNLSD NY4R_RAT 19 1 -
NRSKPLGTPYNFSE NY4R_HUMAN 19 1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
CQDSAELLAFIITT NY4R_MOUSE 34 1 -
CQDSADLLAFIITT NY4R_RAT 34 1 -
CQDSVDVMVFIVTS NY4R_HUMAN 34 1 -

Motif 4 width=16
Element Seqn Id St Int Rpt
LANSTLNDLFHYNHSK NY4R_MOUSE 175 127 -
LANSILNDLFHYNHSK NY4R_RAT 175 127 -
LANSILENVFHKNHSK NY4R_HUMAN 175 127 -

Motif 5 width=11
Element Seqn Id St Int Rpt
KVVEFLEDKVV NY4R_MOUSE 190 -1 -
KVVEFLEDKVV NY4R_RAT 190 -1 -
KALEFLADKVV NY4R_HUMAN 190 -1 -

Motif 6 width=14
Element Seqn Id St Int Rpt
FHAHACSSRAGQMK NY4R_MOUSE 248 47 -
FHTHTCSSRVGQMK NY4R_RAT 248 47 -
FHKGTYSLRAGHMK NY4R_HUMAN 248 47 -

Motif 7 width=12
Element Seqn Id St Int Rpt
WYQEAIPACHGN NY4R_MOUSE 290 28 -
WYQEAIPACHGN NY4R_RAT 290 28 -
WHHEAIPICHGN NY4R_HUMAN 290 28 -

Motif 8 width=12
Element Seqn Id St Int Rpt
FKKDIKALVLTC NY4R_MOUSE 329 27 -
FKKDIKALVLTC NY4R_RAT 329 27 -
FKKEIKALVLTC NY4R_HUMAN 329 27 -

Motif 9 width=14
Element Seqn Id St Int Rpt
PQGESEHLPLSTVH NY4R_MOUSE 345 4 -
PQGEPEPLPLSTVH NY4R_RAT 345 4 -
PLEESEHLPLSTVH NY4R_HUMAN 345 4 -

Motif 10 width=15
Element Seqn Id St Int Rpt
TDLSKGSMRMGSKSN NY4R_MOUSE 359 0 -
TDLSKGSMRMGSKSN NY4R_RAT 359 0 -
TEVSKGSLRLSGRSN NY4R_HUMAN 359 0 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
NTSHFLAPLFPGSLQG NY4R_MOUSE 2 2 -
NTSHLMASLSPAFLQG NY4R_RAT 2 2 -
NTSHLLALLLPKSPQG NY4R_HUMAN 2 2 -

Motif 2 width=14
Element Seqn Id St Int Rpt
NGTNPLDSPYNFSD NY4R_MOUSE 19 1 -
NGTNPLDSLYNLSD NY4R_RAT 19 1 -
NRSKPLGTPYNFSE NY4R_HUMAN 19 1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
CQDSAELLAFIITT NY4R_MOUSE 34 1 -
CQDSADLLAFIITT NY4R_RAT 34 1 -
CQDSVDVMVFIVTS NY4R_HUMAN 34 1 -

Motif 4 width=16
Element Seqn Id St Int Rpt
LANSTLNDLFHYNHSK NY4R_MOUSE 175 127 -
LANSILNDLFHYNHSK NY4R_RAT 175 127 -
LANSILENVFHKNHSK NY4R_HUMAN 175 127 -

Motif 5 width=11
Element Seqn Id St Int Rpt
KVVEFLEDKVV NY4R_MOUSE 190 -1 -
KVVEFLEDKVV NY4R_RAT 190 -1 -
KALEFLADKVV NY4R_HUMAN 190 -1 -

Motif 6 width=14
Element Seqn Id St Int Rpt
FHAHACSSRAGQMK NY4R_MOUSE 248 47 -
FHTHTCSSRVGQMK NY4R_RAT 248 47 -
FHKGTYSLRAGHMK NY4R_HUMAN 248 47 -

Motif 7 width=12
Element Seqn Id St Int Rpt
WYQEAIPACHGN NY4R_MOUSE 290 28 -
WYQEAIPACHGN NY4R_RAT 290 28 -
WHHEAIPICHGN NY4R_HUMAN 290 28 -

Motif 8 width=12
Element Seqn Id St Int Rpt
FKKDIKALVLTC NY4R_MOUSE 329 27 -
FKKDIKALVLTC NY4R_RAT 329 27 -
FKKEIKALVLTC NY4R_HUMAN 329 27 -

Motif 9 width=14
Element Seqn Id St Int Rpt
PQGESEHLPLSTVH NY4R_MOUSE 345 4 -
PQGEPEPLPLSTVH NY4R_RAT 345 4 -
PLEESEHLPLSTVH NY4R_HUMAN 345 4 -

Motif 10 width=15
Element Seqn Id St Int Rpt
TDLSKGSMRMGSKSN NY4R_MOUSE 359 0 -
TDLSKGSMRMGSKSN NY4R_RAT 359 0 -
TEVSKGSLRLSGRSN NY4R_HUMAN 359 0 -