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PR01013

Identifier
NRPEPTIDEY1R  [View Relations]  [View Alignment]  
Accession
PR01013
No. of Motifs
7
Creation Date
30-NOV-1998  (UPDATE 10-JUN-1999)
Title
Neuropeptide Y1 receptor signature 
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01012 NRPEPTIDEYR
INTERPRO; IPR000351
GCRDB; GCR_0295; GCR_0366; GCR_0477; GCR_0641; GCR_0228
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Neuropeptide Y.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.194-198.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Neuropeptide Y (NPY) is one of the most abundant peptides in mammalian
brain, inducing a variety of behavioural effects (e.g., stimulation of food
intake, anxiety, facilitation of learning and memory, and regulation of the
cardiovascular and neuroendocrine systems) [6]. In the periphery, NPY 
stimulates vascular smooth muscle contraction and modulates hormone
secretion. NPY has been implicated in the pathophysiology of hypertension, 
congestive heart failure, affective disorders and appetite regulation [6].
 
Several pharmacologically distinct neuropeptide Y receptors have been 
characterised, designated NPY Y1-Y6. The Y1 receptor is present in smooth
muscle (e.g., intestine and blood vessels), and has also been described
in cell lines. The receptors are believed to have a predominantly post-
synaptic location [6], and are involved in the inhibition of adenylyl
cyclase through a pertussis-toxin-sensitive G protein, probably of the
G0/Gi class [6]. There is also evidence that NPY can stimulate an increase
in intracellular calcium independently of the phosphoinositide pathway [6].
 
NRPEPTIDEY1R is a 7-element fingerprint that provides a signature for
neuropeptide Y1 receptors. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the Y1 receptors but distinguish them from the rest of
the neuropeptide Y family - motif 1 lies at the N-terminus, leading into
TM domain 1; motifs 2 and 3 span the C-terminus of TM domain 4 and the
second external loop; motif 4 lies in the third cytoplasmic loop; motif 5
spans the third external loop; and motifs 6 and 7 reside at the C-terminus. 
A single iteration on OWL30.2 was required to reach convergence, no further
sequences being identified beyond the starting set. Two partial matches 
were found: NY6R_MOUSE is a neuropeptide Y6 receptor that matches motifs 2,
6 and 7; and OAU62122 is an ovine neuropeptide Y1 receptor fragment that 
matches motifs 2 and 3. 
 
An update on SPTR37_9f identified a true set of 5 sequences, and 3
partial matches.
Summary Information
   5 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
75555555
60000000
50000000
40000000
30000000
20101011
1234567
True Positives
NY1R_CANFA    NY1R_HUMAN    NY1R_MOUSE    NY1R_RAT      
NY1R_XENLA
True Positive Partials
Codes involving 2 elements
NY6R_MOUSE NY6R_RABIT
Sequence Titles
NY1R_CANFA  NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) - CANIS FAMILIARIS (DOG). 
NY1R_HUMAN NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) - HOMO SAPIENS (HUMAN).
NY1R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) - MUS MUSCULUS (MOUSE).
NY1R_RAT NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) (FC5) - RATTUS NORVEGICUS (RAT).
NY1R_XENLA NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).

NY6R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 6 (NPY6-R) (PANCREATIC POLYPEPTIDE RECEPTOR 2) (PP2) - MUS MUSCULUS (MOUSE).
NY6R_RABIT NEUROPEPTIDE Y RECEPTOR TYPE 6 (NPY6-R) - ORYCTOLAGUS CUNICULUS (RABBIT).
Scan History
OWL30_2    1  75   NSINGLE    
SPTR37_9f 2 61 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
DCHLPLAVIFTLALAY NY1R_RAT 31 31 -
DCHLPLAVIFTLALAY NY1R_MOUSE 31 31 -
DCHLPLAMIFTLALAY AF005778 31 31 -
DCHLPLAMIFTLALAY AF005779 2 2 -
DCALPLPMIFTLALAY NY1R_XENLA 27 27 -
DCHLPLAMIFTLALAY NY1R_HUMAN 32 32 -

Motif 2 width=16
Element Seqn Id St Int Rpt
YSVLTDEPFKNISLDS NY1R_XENLA 171 128 -
YQVMTDEPFQNVTLDA NY1R_HUMAN 176 128 -
YQVLTDEPFQNVTLDA AF005779 146 128 -
YQVLTDEPFQNVTLDA AF005778 175 128 -
YQILTDEPFQNVSLAA NY1R_RAT 175 128 -
YQILTDEPFQNVSLAA NY1R_MOUSE 175 128 -

Motif 3 width=17
Element Seqn Id St Int Rpt
FKDKYVCFDKFPSDSHR AF005778 191 0 -
FKDKYVCFDKFLSDSHR AF005779 162 0 -
FKDKYVCFDKFPSDSHR NY1R_MOUSE 191 0 -
FKDKYVCFDKFPSDSHR NY1R_RAT 191 0 -
YKDKYVCFDQFPSDSHR NY1R_HUMAN 192 0 -
YIGKYVCLEDFPEDKFR NY1R_XENLA 187 0 -

Motif 4 width=19
Element Seqn Id St Int Rpt
NNMMDKIRDSKYRSSETKR NY1R_RAT 241 33 -
NNMMDKIRDSKYRSSETKR NY1R_MOUSE 241 33 -
NNMMDKMRDNKYRSSETKR AF005778 241 33 -
NNMMDKMRDNKYRSSETKR AF005779 212 33 -
NNMMDKIRDNKYRSSETKR NY1R_XENLA 237 33 -
NNMMDKMRDNKYRSSETKR NY1R_HUMAN 242 33 -

Motif 5 width=12
Element Seqn Id St Int Rpt
WNHQIIATCNHN AF005778 287 27 -
WNHQIIATCNHN AF005779 258 27 -
WNHQIIATCNHN NY1R_MOUSE 287 27 -
WNHQIIATCNHN NY1R_RAT 287 27 -
WNHQIIATCNHN NY1R_HUMAN 288 27 -
WNHEAVATCNHN NY1R_XENLA 283 27 -

Motif 6 width=14
Element Seqn Id St Int Rpt
KNFQRDLQFFFNFC NY1R_HUMAN 325 25 -
KNFQRDLQFFFNFC NY1R_XENLA 320 25 -
KNFQRDLQFFFNFC AF005778 324 25 -
KNFQRDLQFFFNFC AF005779 295 25 -
KNFQRDLQFFFNFC NY1R_MOUSE 324 25 -
KNFQRDLQFFFNFC NY1R_RAT 324 25 -

Motif 7 width=18
Element Seqn Id St Int Rpt
CDFRSRDDDYETIAMSTM NY1R_MOUSE 337 -1 -
CDFRSRDDDYETIAMSTM AF005779 308 -1 -
CDFRSREDDYETIAMSTM NY1R_XENLA 333 -1 -
CDFRSRDDDYETIAMSTM NY1R_HUMAN 338 -1 -
CDFRSRDDDYETIAMSTM NY1R_RAT 337 -1 -
CDFRSRDDDYETIAMSTM AF005778 337 -1 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
DCHLPLAMIFTLALAY NY1R_CANFA 31 31 -
DCHLPLAVIFTLALAY NY1R_MOUSE 31 31 -
DCHLPLAVIFTLALAY NY1R_RAT 31 31 -
DCHLPLAMIFTLALAY NY1R_HUMAN 32 32 -
DCALPLPMIFTLALAY NY1R_XENLA 27 27 -

Motif 2 width=16
Element Seqn Id St Int Rpt
YQVLTDEPFQNVTLDA NY1R_CANFA 175 128 -
YQILTDEPFQNVSLAA NY1R_MOUSE 175 128 -
YQILTDEPFQNVSLAA NY1R_RAT 175 128 -
YQVMTDEPFQNVTLDA NY1R_HUMAN 176 128 -
YSVLTDEPFKNISLDS NY1R_XENLA 171 128 -

Motif 3 width=17
Element Seqn Id St Int Rpt
FKDKYVCFDKFPSDSHR NY1R_CANFA 191 0 -
FKDKYVCFDKFPSDSHR NY1R_MOUSE 191 0 -
FKDKYVCFDKFPSDSHR NY1R_RAT 191 0 -
YKDKYVCFDQFPSDSHR NY1R_HUMAN 192 0 -
YIGKYVCLEDFPEDKFR NY1R_XENLA 187 0 -

Motif 4 width=19
Element Seqn Id St Int Rpt
NNMMDKMRDNKYRSSETKR NY1R_CANFA 241 33 -
NNMMDKIRDSKYRSSETKR NY1R_MOUSE 241 33 -
NNMMDKIRDSKYRSSETKR NY1R_RAT 241 33 -
NNMMDKMRDNKYRSSETKR NY1R_HUMAN 242 33 -
NNMMDKIRDNKYRSSETKR NY1R_XENLA 237 33 -

Motif 5 width=12
Element Seqn Id St Int Rpt
WNHQIIATCNHN NY1R_CANFA 287 27 -
WNHQIIATCNHN NY1R_MOUSE 287 27 -
WNHQIIATCNHN NY1R_RAT 287 27 -
WNHQIIATCNHN NY1R_HUMAN 288 27 -
WNHEAVATCNHN NY1R_XENLA 283 27 -

Motif 6 width=14
Element Seqn Id St Int Rpt
KNFQRDLQFFFNFC NY1R_CANFA 324 25 -
KNFQRDLQFFFNFC NY1R_MOUSE 324 25 -
KNFQRDLQFFFNFC NY1R_RAT 324 25 -
KNFQRDLQFFFNFC NY1R_HUMAN 325 25 -
KNFQRDLQFFFNFC NY1R_XENLA 320 25 -

Motif 7 width=18
Element Seqn Id St Int Rpt
CDFRSRDDDYETIAMSTM NY1R_CANFA 337 -1 -
CDFRSRDDDYETIAMSTM NY1R_MOUSE 337 -1 -
CDFRSRDDDYETIAMSTM NY1R_RAT 337 -1 -
CDFRSRDDDYETIAMSTM NY1R_HUMAN 338 -1 -
CDFRSREDDYETIAMSTM NY1R_XENLA 333 -1 -