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PR01012

Identifier
NRPEPTIDEYR  [View Relations]  [View Alignment]  
Accession
PR01012
No. of Motifs
5
Creation Date
30-NOV-1998  (UPDATE 03-JUL-1999)
Title
Neuropeptide Y receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01013 NRPEPTIDEY1R; PR01014 NRPEPTIDEY2R; PR01015 NRPEPTIDEY4R
PRINTS; PR01016 NRPEPTIDEY5R; PR01017 NRPEPTIDEY6R
INTERPRO; IPR000611
GCRDB; GCR_0295; GCR_0366; GCR_0477; GCR_0641; GCR_0228
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Neuropeptide Y.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.194-198.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Neuropeptide Y (NPY) is one of the most abundant peptides in mammalian
brain, inducing a variety of behavioural effects (e.g., stimulation of food
intake, anxiety, facilitation of learning and memory, and regulation of the
cardiovascular and neuroendocrine systems) [6]. In the periphery, NPY 
stimulates vascular smooth muscle contraction and modulates hormone
secretion. NPY has been implicated in the pathophysiology of hypertension, 
congestive heart failure, affective disorders and appetite regulation [6].
 
NRPEPTIDEYR is a 5-element fingerprint that provides a signature for
neuropeptide Y receptors. The fingerprint was derived from an initial
alignment of 11 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the neuropeptide Y receptors but distinguish them from the
rest of the rhodopsin-like GPCR superfamily - motif 1 spans the first 
cytoplasmic loop; motif 2 spans the first external loop; motif 3 encodes
the N-terminal portion of TM domain 3; motif 4 spans the central portion 
of TM domain 7; and motif 5 lies at the C-terminus. Two iterations on
OWL30.2 were required to reach convergence, at which point a true set 
comprising 20 sequences was identified. Several partial matches were also
found, all of which are superfamily members that match two motifs. 
 
An update on SPTR37_9f identified a true set of 22 sequences, and 9
partial matches.
Summary Information
  22 codes involving  5 elements
0 codes involving 4 elements
2 codes involving 3 elements
7 codes involving 2 elements
Composite Feature Index
52222222222
400000
322200
243403
12345
True Positives
NY1R_CANFA    NY1R_HUMAN    NY1R_MOUSE    NY1R_RAT      
NY1R_XENLA NY2R_BOVIN NY2R_HUMAN NY2R_MOUSE
NY2R_PIG NY4R_HUMAN NY4R_MOUSE NY4R_RAT
NY5R_CANFA NY5R_HUMAN NY5R_MOUSE NY5R_RAT
NY6R_MOUSE NY6R_RABIT O35380 O57463
O73733 O73734
True Positive Partials
Codes involving 3 elements
Q99463 Q99647
Codes involving 2 elements
CCKR_CAVPO CCKR_HUMAN CCKR_RAT O08786
SSR1_HUMAN SSR1_MOUSE SSR1_RAT
Sequence Titles
NY1R_CANFA  NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) - CANIS FAMILIARIS (DOG). 
NY1R_HUMAN NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) - HOMO SAPIENS (HUMAN).
NY1R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) - MUS MUSCULUS (MOUSE).
NY1R_RAT NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) (FC5) - RATTUS NORVEGICUS (RAT).
NY1R_XENLA NEUROPEPTIDE Y RECEPTOR TYPE 1 (NPY1-R) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
NY2R_BOVIN NEUROPEPTIDE Y RECEPTOR TYPE 2 (NPY2-R) - BOS TAURUS (BOVINE).
NY2R_HUMAN NEUROPEPTIDE Y RECEPTOR TYPE 2 (NPY2-R) - HOMO SAPIENS (HUMAN).
NY2R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 2 (NPY2-R) - MUS MUSCULUS (MOUSE).
NY2R_PIG NEUROPEPTIDE Y RECEPTOR TYPE 2 (NPY2-R) - SUS SCROFA (PIG).
NY4R_HUMAN Neuropeptide Y receptor type 4 (NPY4-R) (Pancreatic polypeptide receptor 1) (PP1) - Homo sapiens (Human).
NY4R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 4 (NPY4-R) (PANCREATIC POLYPEPTIDE RECEPTOR 1) (PP1) (NPYR-D) - MUS MUSCULUS (MOUSE).
NY4R_RAT NEUROPEPTIDE Y RECEPTOR TYPE 4 (NPY4-R) (PANCREATIC POLYPEPTIDE RECEPTOR 1) (PP1) - RATTUS NORVEGICUS (RAT).
NY5R_CANFA NEUROPEPTIDE Y RECEPTOR TYPE 5 (NPY5-R) (Y5 RECEPTOR) (NPYY5) - CANIS FAMILIARIS (DOG).
NY5R_HUMAN NEUROPEPTIDE Y RECEPTOR TYPE 5 (NPY5-R) (Y5 RECEPTOR) (NPYY5) - HOMO SAPIENS (HUMAN).
NY5R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 5 (NPY5-R) (Y5 RECEPTOR) - MUS MUSCULUS (MOUSE).
NY5R_RAT NEUROPEPTIDE Y RECEPTOR TYPE 5 (NPY5-R) (Y5 RECEPTOR) - RATTUS NORVEGICUS (RAT).
NY6R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 6 (NPY6-R) (PANCREATIC POLYPEPTIDE RECEPTOR 2) (PP2) - MUS MUSCULUS (MOUSE).
NY6R_RABIT NEUROPEPTIDE Y RECEPTOR TYPE 6 (NPY6-R) - ORYCTOLAGUS CUNICULUS (RABBIT).
O35380 NEUROPEPTIDE Y Y5 RECEPTOR - MUS MUSCULUS (MOUSE).
O57463 NEUROPEPTIDE Y /PEPTIDE YY RECEPTOR YB - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
O73733 NEUROPEPTIDE Y/PEPTIDE YY RECEPTOR YA - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
O73734 NEUROPEPTIDE Y/PEPTIDE YY RECEPTOR YC - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).

Q99463 Y6 ENCODING PROTEIN - HOMO SAPIENS (HUMAN).
Q99647 TRUNCATED PANCREATIC POLYPEPTIDE RECEPTOR PP2 - HOMO SAPIENS (HUMAN).

CCKR_CAVPO Cholecystokinin type A receptor (CCK-A receptor) (CCK-AR) - Cavia porcellus (Guinea pig).
CCKR_HUMAN Cholecystokinin type A receptor (CCK-A receptor) (CCK-AR) - Homo sapiens (Human).
CCKR_RAT Cholecystokinin type A receptor (CCK-A receptor) (CCK-AR) - Rattus norvegicus (Rat).
O08786 CHOLECYSTOKININ A RECEPTOR (CHOLECYSTOKININ TYPE-A RECEPTOR) - MUS MUSCULUS (MOUSE).
SSR1_HUMAN Somatostatin receptor type 1 (SS1R) (SRIF-2) - Homo sapiens (Human).
SSR1_MOUSE Somatostatin receptor type 1 (SS1R) (SRIF-2) - Mus musculus (Mouse).
SSR1_RAT Somatostatin receptor type 1 (SS1R) (SRIF-2) - Rattus norvegicus (Rat).
Scan History
OWL30_2    2  100  NSINGLE    
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
KQKEMRNVTNILI NY1R_XENLA 62 62 -
KQKEMRNVTNILI NY1R_RAT 66 66 -
KQKEMRNVTNILI NY1R_MOUSE 66 66 -
KQKEMRNVTNILI NY1R_HUMAN 67 67 -
RQKEKSNVTNLLI NY4R_MOUSE 68 68 -
RQKEKSNVTNLLI NY4R_RAT 68 68 -
RQKEKANVTNLLI NY4R_HUMAN 68 68 -
KKRNQKTTVNFLI NY5R_RAT 79 79 -
KKRNQKTTVNFLI NY5R_HUMAN 78 78 -
KFKSMRTVTNFFI NY2R_HUMAN 77 77 -
KFKSMRTVTNFFI NY2R_BOVIN 80 80 -

Motif 2 width=13
Element Seqn Id St Int Rpt
TLIYTLMDHWIFG NY1R_XENLA 92 17 -
TFVYTLMDHWVFG NY1R_RAT 96 17 -
TFVYTLMDHWVFG NY1R_MOUSE 96 17 -
TFVYTLMDHWVFG NY1R_HUMAN 97 17 -
TVTYTIMDYWIFG NY4R_MOUSE 98 17 -
TVTYTIMDYWIFG NY4R_RAT 98 17 -
TAVYTIMDYWIFG NY4R_HUMAN 98 17 -
TLTSVLLDQWMFG NY5R_RAT 109 17 -
TLTSVLLDQWMFG NY5R_HUMAN 108 17 -
TLTYTLMGEWKMG NY2R_HUMAN 107 17 -
TLTYTLMGEWKMG NY2R_BOVIN 110 17 -

Motif 3 width=16
Element Seqn Id St Int Rpt
QCVSVTVSIFSLVLIA NY1R_XENLA 115 10 -
QCVSITVSIFSLVLIA NY1R_RAT 119 10 -
QCVSITVSIFSLVLIA NY1R_MOUSE 119 10 -
QCVSITVSIFSLVLIA NY1R_HUMAN 120 10 -
QCMSVTVSILSLVLVA NY4R_MOUSE 121 10 -
QCMSVTVSILSLVLVA NY4R_RAT 121 10 -
QCMSVTVSILSLVLVA NY4R_HUMAN 121 10 -
QCVSVLVSTLILISIA NY5R_RAT 132 10 -
QCVSVLVSTLILISIA NY5R_HUMAN 131 10 -
QGLAVQVSTITLTVIA NY2R_HUMAN 130 10 -
QGLAVQVSTITLTVIA NY2R_BOVIN 133 10 -

Motif 4 width=10
Element Seqn Id St Int Rpt
LTAMISTCVN NY1R_XENLA 302 171 -
LTAMISTCVN NY1R_RAT 306 171 -
LTAMISTCVN NY1R_MOUSE 306 171 -
LTAMISTCVN NY1R_HUMAN 307 171 -
LLAMASTCVN NY4R_MOUSE 309 172 -
LFAMASTCVN NY4R_RAT 309 172 -
LLAMASTCVN NY4R_HUMAN 309 172 -
LLGMMSCCLN NY5R_RAT 422 274 -
LLGMMSCCLN NY5R_HUMAN 422 275 -
IIAMCSTFAN NY2R_HUMAN 312 166 -
IIAMCSTFAN NY2R_BOVIN 315 166 -

Motif 5 width=14
Element Seqn Id St Int Rpt
IFYGFLNKNFQRDL NY1R_XENLA 313 1 -
IFYGFLNKNFQRDL NY1R_RAT 317 1 -
IFYGFLNKNFQRDL NY1R_MOUSE 317 1 -
IFYGFLNKNFQRDL NY1R_HUMAN 318 1 -
FIYGFLNINFKKDI NY4R_MOUSE 320 1 -
FIYGFLNINFKKDI NY4R_RAT 320 1 -
FIYGFLNTNFKKEI NY4R_HUMAN 320 1 -
ILYGFLNNGIKADL NY5R_RAT 433 1 -
ILYGFLNNGIKADL NY5R_HUMAN 433 1 -
LLYGWMNSNYRKAF NY2R_HUMAN 323 1 -
LLYGWMNSNYRKAF NY2R_BOVIN 326 1 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
KQKEMRNVTNILI NY1R_XENLA 62 62 -
KQKEMRNVTNILI NY1R_RAT 66 66 -
KQKEMRNVTNILI NY1R_MOUSE 66 66 -
KQKEMRNVTNILI NY1R_HUMAN 67 67 -
KQKEMRNVTNILI NY1R_CANFA 66 66 -
RQKEMRNVTNILI O73734 57 57 -
KQREAQNVTNILI NY6R_MOUSE 63 63 -
RQKEMRNVTNIFI O57463 52 52 -
RQKEKSNVTNLLI NY4R_MOUSE 68 68 -
KQREAQNVTNILI NY6R_RABIT 63 63 -
RQKEKSNVTNLLI NY4R_RAT 68 68 -
HQRDPPNVTSILI O73733 69 69 -
RQKEKANVTNLLI NY4R_HUMAN 68 68 -
KKRNQKTTVNFLI O35380 89 89 -
KKRNQKTTVNFLI NY5R_RAT 79 79 -
KKRNQKTTVNFLI NY5R_MOUSE 89 89 -
KKRNQKTTVNFLI NY5R_HUMAN 78 78 -
RKRNQKTMVNFLI NY5R_CANFA 68 68 -
KFKSMRTVTNFFI NY2R_PIG 78 78 -
KFKSMRTVTNFFI NY2R_MOUSE 81 81 -
KFKSMRTVTNFFI NY2R_HUMAN 77 77 -
KFKSMRTVTNFFI NY2R_BOVIN 80 80 -

Motif 2 width=13
Element Seqn Id St Int Rpt
TLIYTLMDHWIFG NY1R_XENLA 92 17 -
TFVYTLMDHWVFG NY1R_RAT 96 17 -
TFVYTLMDHWVFG NY1R_MOUSE 96 17 -
TFVYTLMDHWVFG NY1R_HUMAN 97 17 -
TFVYTLMDHWVFG NY1R_CANFA 96 17 -
TVIYTLMDRWILG O73734 87 17 -
TVIYTLMDHWVFG NY6R_MOUSE 93 17 -
TIIYTLMDRWILG O57463 82 17 -
TVTYTIMDYWIFG NY4R_MOUSE 98 17 -
TAIYTLMDRWIFG NY6R_RABIT 93 17 -
TVTYTIMDYWIFG NY4R_RAT 98 17 -
TVVYTLMDHWIFG O73733 99 17 -
TAVYTIMDYWIFG NY4R_HUMAN 98 17 -
TLTSVLLDQWMFG O35380 119 17 -
TLTSVLLDQWMFG NY5R_RAT 109 17 -
TLTSVLLDQWMFG NY5R_MOUSE 119 17 -
TLTSVLLDQWMFG NY5R_HUMAN 108 17 -
TLTSVLLDQWMFG NY5R_CANFA 98 17 -
TLTYTLMGEWKMG NY2R_PIG 108 17 -
TLTYTLMGEWKMG NY2R_MOUSE 111 17 -
TLTYTLMGEWKMG NY2R_HUMAN 107 17 -
TLTYTLMGEWKMG NY2R_BOVIN 110 17 -

Motif 3 width=16
Element Seqn Id St Int Rpt
QCVSVTVSIFSLVLIA NY1R_XENLA 115 10 -
QCVSITVSIFSLVLIA NY1R_RAT 119 10 -
QCVSITVSIFSLVLIA NY1R_MOUSE 119 10 -
QCVSITVSIFSLVLIA NY1R_HUMAN 120 10 -
QCVSITVSIFSLVLIA NY1R_CANFA 119 10 -
QCMSVTVSIFSLVLIA O73734 110 10 -
QSVSVSVSIFSLVLIA NY6R_MOUSE 116 10 -
QCMSVTVSIFSMVLIA O57463 105 10 -
QCMSVTVSILSLVLVA NY4R_MOUSE 121 10 -
QSVSISVSIFSLVLIA NY6R_RABIT 116 10 -
QCMSVTVSILSLVLVA NY4R_RAT 121 10 -
QCVSVTVSVLSLVLIA O73733 122 10 -
QCMSVTVSILSLVLVA NY4R_HUMAN 121 10 -
QCVSVLVSTLILISIA O35380 142 10 -
QCVSVLVSTLILISIA NY5R_RAT 132 10 -
QCVSVLVSTLILISIA NY5R_MOUSE 142 10 -
QCVSVLVSTLILISIA NY5R_HUMAN 131 10 -
QCVSVLVSTLILISIA NY5R_CANFA 121 10 -
QGLAVQVSTITLTVIA NY2R_PIG 131 10 -
QGLAVQVSTITLTVIA NY2R_MOUSE 134 10 -
QGLAVQVSTITLTVIA NY2R_HUMAN 130 10 -
QGLAVQVSTITLTVIA NY2R_BOVIN 133 10 -

Motif 4 width=10
Element Seqn Id St Int Rpt
LTAMISTCVN NY1R_XENLA 302 171 -
LTAMISTCVN NY1R_RAT 306 171 -
LTAMISTCVN NY1R_MOUSE 306 171 -
LTAMISTCVN NY1R_HUMAN 307 171 -
LTAMISTCVN NY1R_CANFA 306 171 -
LTAMASTCVN O73734 300 174 -
LIAMVSTCIN NY6R_MOUSE 305 173 -
LTAMASTCVN O57463 295 174 -
LLAMASTCVN NY4R_MOUSE 309 172 -
LVAMVSTCIN NY6R_RABIT 305 173 -
LFAMASTCVN NY4R_RAT 309 172 -
LLAMSSTCVN O73733 313 175 -
LLAMASTCVN NY4R_HUMAN 309 172 -
LLGMMSCCLN O35380 432 274 -
LLGMMSCCLN NY5R_RAT 422 274 -
LLGMMSCCLN NY5R_MOUSE 432 274 -
LLGMMSCCLN NY5R_HUMAN 422 275 -
LLGMMSCCLN NY5R_CANFA 412 275 -
IIAMCSTFAN NY2R_PIG 314 167 -
IIAMCSTFAN NY2R_MOUSE 316 166 -
IIAMCSTFAN NY2R_HUMAN 312 166 -
IIAMCSTFAN NY2R_BOVIN 315 166 -

Motif 5 width=14
Element Seqn Id St Int Rpt
IFYGFLNKNFQRDL NY1R_XENLA 313 1 -
IFYGFLNKNFQRDL NY1R_RAT 317 1 -
IFYGFLNKNFQRDL NY1R_MOUSE 317 1 -
IFYGFLNKNFQRDL NY1R_HUMAN 318 1 -
IFYGFLNKNFQRDL NY1R_CANFA 317 1 -
VVYGFLNTNFQKEL O73734 311 1 -
LFYGFLNKNFQKDL NY6R_MOUSE 316 1 -
VIYGFLNNNFQKEL O57463 306 1 -
FIYGFLNINFKKDI NY4R_MOUSE 320 1 -
LFYGFLNRNFQKDL NY6R_RABIT 316 1 -
FIYGFLNINFKKDI NY4R_RAT 320 1 -
IIYGFLNSNFRKDV O73733 324 1 -
FIYGFLNTNFKKEI NY4R_HUMAN 320 1 -
ILYGFLNNGIKADL O35380 443 1 -
ILYGFLNNGIKADL NY5R_RAT 433 1 -
ILYGFLNNGIKADL NY5R_MOUSE 443 1 -
ILYGFLNNGIKADL NY5R_HUMAN 433 1 -
ILYGFLNNGIKADL NY5R_CANFA 423 1 -
LLYGWMNSNYRKAF NY2R_PIG 325 1 -
LLYGWMNSNYRKAF NY2R_MOUSE 327 1 -
LLYGWMNSNYRKAF NY2R_HUMAN 323 1 -
LLYGWMNSNYRKAF NY2R_BOVIN 326 1 -