SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00994

Identifier
BRADYKINNB2R  [View Relations]  [View Alignment]  
Accession
PR00994
No. of Motifs
8
Creation Date
25-NOV-1998  (UPDATE 14-JUN-1999)
Title
B2 bradykinin receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00425 BRADYKININR
INTERPRO; IPR001504
GCRDB; GCR_0170; GCR_0171; GCR_0172; GCR_0775; GCR_0795; GCR_0302

GCRDB; GCR_0508
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL,
Bradykinin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp67-70.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Bradykinins (BKs) are a family of short, structurally similar peptides that 
activate sensory fibres, contract venous smooth muscle, stimulate release
of cytokines, induce connective tissue proliferation and mediate endo-
thelium-dependent vasodilation [6]. BK antagonists are of potential use
in the treatment of inflammation, asthma, mild pain and endotoxic shock.
BK receptors are widespread in peripheral tissues, and at least 3 different
receptor subtypes have been proposed. Of these, B2 is the predominant
subtype, mediating slow contraction of various smooth muscles (including
veins, intestine, uterus, trachea and lung), inducing endothelium-dependent
relaxation of arteries and arterioles, and stimulating natriuresis/diuresis
in kidney. BK also induces hyperalgesia through activation of B2 receptors
in sensory nerve fibres and dorsal root ganglion neurons [6].
 
BRADYKINNB2R is an 8-element fingerprint that provides a signature for 
the B2 bradykinin receptors. The fingerprint was derived from an initial 
alignment of 5 sequences: the motifs were drawn from conserved sections 
within either loop or TM regions, focusing on those areas of the alignment 
that characterise the B2 bradykinin receptors but distinguish them from the 
rest of the bradykinin receptor family - motif 1 lies at the N-terminus;
motifs 2 and 3 span the C-terminus of TM domain 4, leading into the second
external loop; motif 4 spans the third cytoplasmic loop; motif 5 lies in
the third external loop; and motifs 6-8 span the C-terminus. A single
iteration on OWL30.2 was required to reach convergence, no further sequences
being identified beyond the starting set. 
 
An update on SPTR37_9f identified a true set of 5 sequences, and 1
partial match.
Summary Information
   5 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
855555555
700000000
600000000
500000000
400000000
301010010
200000000
12345678
True Positives
BRB2_HUMAN    BRB2_MOUSE    BRB2_RABIT    BRB2_RAT      
O70526
True Positive Partials
Codes involving 3 elements
O42402
Sequence Titles
BRB2_HUMAN  B2 BRADYKININ RECEPTOR (BK-2 RECEPTOR) - HOMO SAPIENS (HUMAN). 
BRB2_MOUSE B2 BRADYKININ RECEPTOR (BK-2 RECEPTOR) (B2R) - MUS MUSCULUS (MOUSE).
BRB2_RABIT B2 BRADYKININ RECEPTOR (BK-2 RECEPTOR) - ORYCTOLAGUS CUNICULUS (RABBIT).
BRB2_RAT B2 BRADYKININ RECEPTOR (BK-2 RECEPTOR) - RATTUS NORVEGICUS (RAT).
O70526 BRADYKININ B2 RECEPTOR - CAVIA PORCELLUS (GUINEA PIG).

O42402 ORNITHOKININ RECEPTOR - GALLUS GALLUS (CHICKEN).
Scan History
OWL30_2    1  100  NSINGLE    
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
CPQVEWLGWLNTIQP BRB2_HUMAN 47 47 -
CPDTEWWSWLNAIQA BRB2_RAT 22 22 -
CPDTEWWSWLNAIQA BRB2_MOUSE 22 22 -
CPNTEWSGWLNVIQA OCU33334 23 23 -
CLDAEWWSWLNTIQA CPAJ3243 21 21 -

Motif 2 width=13
Element Seqn Id St Int Rpt
SCTLLLSSPMLVF BRB2_RAT 158 121 -
GCALLLSSPMLVF CPAJ3243 157 121 -
GCTLLLSSPMLVF OCU33334 159 121 -
GCTLLLSSPMLVF BRB2_MOUSE 158 121 -
GCTLLLSSPMLVF BRB2_HUMAN 183 121 -

Motif 3 width=13
Element Seqn Id St Int Rpt
TMREYSEEGHNVT BRB2_MOUSE 172 1 -
TMKEYSDEGHNVT BRB2_HUMAN 197 1 -
TMKDYREEGHNVT BRB2_RAT 172 1 -
TMKDYRDEGYNVT OCU33334 173 1 -
TMKDYRDEGHNVT CPAJ3243 171 1 -

Motif 4 width=17
Element Seqn Id St Int Rpt
RNNEMQKFKEIQTERRA CPAJ3243 226 42 -
RNNEMQKFKEIQTERRA BRB2_HUMAN 252 42 -
RNNEMKKFKEVQTEKKA BRB2_RAT 227 42 -
RNNEMKKFKEVQTERKA BRB2_MOUSE 227 42 -
RNNEMQKFKEIQTERRA OCU33334 228 42 -

Motif 5 width=19
Element Seqn Id St Int Rpt
FQVSTFLDTLLKLGVLSSC OCU33334 262 17 -
FQISTFLDTLLRLGVLSGC BRB2_MOUSE 261 17 -
FQISTFLDTLLRLGVLSGC BRB2_RAT 261 17 -
FQISTFLDTLHRLGILSSC BRB2_HUMAN 286 17 -
FQIGTFLDTLRLLGFLPGC CPAJ3243 260 17 -

Motif 6 width=20
Element Seqn Id St Int Rpt
KRFRKKSREVYHGLCRSGGC CPAJ3243 310 31 -
KRFRKKSREVYRVLCQKGGC BRB2_MOUSE 312 32 -
KRFRKKSREVYQAICRKGGC BRB2_RAT 312 32 -
KRFRKKSREVYRAACPKAGC OCU33334 313 32 -
KRFRKKSWEVYQGVCQKGGC BRB2_HUMAN 337 32 -

Motif 7 width=17
Element Seqn Id St Int Rpt
CVLEPVQAESSMGTLRT OCU33334 332 -1 -
CVSEPAQSENSMGTLRT CPAJ3243 329 -1 -
CRSEPIQMENSMGTLRT BRB2_HUMAN 356 -1 -
CMGEPVQMENSMGTLRT BRB2_MOUSE 331 -1 -
CMGESVQMENSMGTLRT BRB2_RAT 331 -1 -

Motif 8 width=16
Element Seqn Id St Int Rpt
TSISVDRQIHKLQDWA CPAJ3243 345 -1 -
TSISVERQIHKLQDWA BRB2_HUMAN 372 -1 -
TSISVDRQIHKLQDWA BRB2_RAT 347 -1 -
TSISVERQIHKLQDWA BRB2_MOUSE 347 -1 -
TSISVERQIHKLPEWT OCU33334 348 -1 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
CPDTEWWSWLNAIQA BRB2_MOUSE 22 22 -
CPDTEWWSWLNAIQA BRB2_RAT 22 22 -
CPQVEWLGWLNTIQP BRB2_HUMAN 47 47 -
CLDAEWWSWLNTIQA O70526 21 21 -
CPNTEWSGWLNVIQA BRB2_RABIT 23 23 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GCTLLLSSPMLVF BRB2_MOUSE 158 121 -
SCTLLLSSPMLVF BRB2_RAT 158 121 -
GCTLLLSSPMLVF BRB2_HUMAN 183 121 -
GCALLLSSPMLVF O70526 157 121 -
GCTLLLSSPMLVF BRB2_RABIT 159 121 -

Motif 3 width=13
Element Seqn Id St Int Rpt
TMREYSEEGHNVT BRB2_MOUSE 172 1 -
TMKDYREEGHNVT BRB2_RAT 172 1 -
TMKEYSDEGHNVT BRB2_HUMAN 197 1 -
TMKDYRDEGHNVT O70526 171 1 -
TMKDYRDEGYNVT BRB2_RABIT 173 1 -

Motif 4 width=17
Element Seqn Id St Int Rpt
RNNEMKKFKEVQTERKA BRB2_MOUSE 227 42 -
RNNEMKKFKEVQTEKKA BRB2_RAT 227 42 -
RNNEMQKFKEIQTERRA BRB2_HUMAN 252 42 -
RNNEMQKFKEIQTERRA O70526 226 42 -
RNNEMQKFKEIQTERRA BRB2_RABIT 228 42 -

Motif 5 width=19
Element Seqn Id St Int Rpt
FQISTFLDTLLRLGVLSGC BRB2_MOUSE 261 17 -
FQISTFLDTLLRLGVLSGC BRB2_RAT 261 17 -
FQISTFLDTLHRLGILSSC BRB2_HUMAN 286 17 -
FQIGTFLDTLRLLGFLPGC O70526 260 17 -
FQVSTFLDTLLKLGVLSSC BRB2_RABIT 262 17 -

Motif 6 width=20
Element Seqn Id St Int Rpt
KRFRKKSREVYRVLCQKGGC BRB2_MOUSE 312 32 -
KRFRKKSREVYQAICRKGGC BRB2_RAT 312 32 -
KRFRKKSWEVYQGVCQKGGC BRB2_HUMAN 337 32 -
KRFRKKSREVYHGLCRSGGC O70526 310 31 -
KRFRKKSREVYRAACPKAGC BRB2_RABIT 313 32 -

Motif 7 width=17
Element Seqn Id St Int Rpt
CMGEPVQMENSMGTLRT BRB2_MOUSE 331 -1 -
CMGESVQMENSMGTLRT BRB2_RAT 331 -1 -
CRSEPIQMENSMGTLRT BRB2_HUMAN 356 -1 -
CVSEPAQSENSMGTLRT O70526 329 -1 -
CVLEPVQAESSMGTLRT BRB2_RABIT 332 -1 -

Motif 8 width=16
Element Seqn Id St Int Rpt
TSISVERQIHKLQDWA BRB2_MOUSE 347 -1 -
TSISVDRQIHKLQDWA BRB2_RAT 347 -1 -
TSISVERQIHKLQDWA BRB2_HUMAN 372 -1 -
TSISVDRQIHKLQDWA O70526 345 -1 -
TSISVERQIHKLPEWT BRB2_RABIT 348 -1 -