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PR00989

Identifier
ADENOKINASE  [View Relations]  [View Alignment]  
Accession
PR00989
No. of Motifs
5
Creation Date
05-OCT-1998  (UPDATE 06-JUN-1999)
Title
Adenosine kinase signature
Database References

PROSITE; PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2
BLOCKS; BL00583
INTERPRO; IPR001805
Literature References
1. SPYCHALA, J., DATTA, N.S., TAKABAYASHI, K., DATTA, M., FOX, I.H.,
GRIBBIN, T. AND MITCHELL, B.S.
Cloning of human adenosine kinase cDNA: Sequence similarity to microbrial
ribokinases and fructokinases.
PROC.NATL.ACAD.SCI.U.S.A. 93 1232-1237 (1996).
 
2. SINGH, B., HAO, W.H., WU, Z.C., EIGL, B. AND GUPTA, R.S.
Cloning and characterization of cDNA for adenosine kinase from mammalian
Chinese hamster ovary cells involve structural alterations in the gene.
EUR.J.BIOCHEMISTRY 241 564-571 (1996).
 
3. MCNALLY, T., HELFRICH, R.J., COWART, M., DORWIN, S.A., MEUTH, J.L.,
IDLER, K.B., KLUTE, K.A., SIMMER, R.L., KOWALUK, E.A. AND HALBERT, D.N.
Cloning and expressions of the adenosine kinase gene from rat and human
tissues.
BIOCHEM.BIOPHYS.RES.COMMUN. 231 645-650 (1997).
 
4. KULKARNI, J.S., PRZYWARA, D.A., WAKADE, T.D. AND WAKADE, A.R.
Adenosine induces apoptosis by inhibiting mRNA and protein synthesis in
chick embryonic sympathetic neurons.
NEUROSCI.LETT. 248 187-190 (1998).
 
5. KEIL, G.J AND SALTER, M.W.
Multiple roles of ATP and adenosine in somatosensory processing: Therapeutic
implications.
DRUG DEV.RES. 39 279-288 (1996).
 
6. SAWYNOK, J.
Adenosine receptor activation and nociception.
EUR.J.PHARMACOL. 347 1-11 (1998).
 
7. FIRESTEIN, G.S.
Anti-inflammatory effects of adenosine kinase inhibitors in acute and
chronic inflammation.
DRUG DEV.RES. 39 371-376 (1996).

Documentation
Adenosine kinase (ADK) phosphorylates adenosine and other related nucleosides
[1,2]. It is exclusive to eukaryotes [3] and is a key enzyme in the purine
salvage pathway.
 
ADK catalyses the reaction:
 
		  Adenosine + ATP = ADP + AMP	     [1]
 
This reaction prevents toxic levels of adenosine building up within the
cell. Experiments have been carried out on chick embryonic neurons to
document the toxicity of adenosine, and the associated requirement for ADK.
[4]
 
Adenosine and ATP have been connected with pain, and the control of pain
[5,6]. Associated with this, adenosine has also been shown to have a number
of anti-inflammatory activities [7].
 
Human ADK bears little sequence similarity to other mammalian nucleoside
kinases, but does share high similarity with some microbrial ribokinases and
fructokinases, suggesting possible evolutionary connections [1,2].
 
ADENOKINASE is a 5-element fingerprint that provides a signature for
adenosine kinases. The fingerprint was derived from an initial alignment of
6 sequences: the motifs were drawn from conserved regions spanning the
alignment N- and C-termini. Two iterations on OWL30.2 were required to 
reach convergence, at which point a true set comprising 12 sequences was 
identified.
 
An update on SPTR37_9f identified a true set of 8 sequences.
Summary Information
8 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
588888
400000
300000
200000
12345
True Positives
ADK_CRIGR     ADK_HUMAN     ADK_RAT       ADK_YEAST     
O00741 O00742 O49923 Q93934
Sequence Titles
ADK_CRIGR   ADENOSINE KINASE (EC 2.7.1.20) (AK) (ADENOSINE 5'-PHOSPHOTRANSFERASE) - CRICETULUS GRISEUS (CHINESE HAMSTER). 
ADK_HUMAN ADENOSINE KINASE (EC 2.7.1.20) (AK) (ADENOSINE 5'-PHOSPHOTRANSFERASE) - HOMO SAPIENS (HUMAN).
ADK_RAT ADENOSINE KINASE (EC 2.7.1.20) (AK) (ADENOSINE 5'-PHOSPHOTRANSFERASE) - RATTUS NORVEGICUS (RAT).
ADK_YEAST PUTATIVE ADENOSINE KINASE (EC 2.7.1.20) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
O00741 ADENOSINE KINASE LONG FORM (EC 2.7.1.20) - HOMO SAPIENS (HUMAN).
O00742 ADENOSINE KINASE SHORT FORM (EC 2.7.1.20) - HOMO SAPIENS (HUMAN).
O49923 ADENOSINE KINASE (EC 2.7.1.20) - PHYSCOMITRELLA PATENS (MOSS).
Q93934 R07H5.8 PROTEIN - CAENORHABDITIS ELEGANS.
Scan History
OWL30_2    2  50   NSINGLE    
SPTR37_9f 2 22 NSINGLE
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
NSMKVAQWMI ADK_RAT 57 57 -
NSIKVAQWMI ADK_CRIGR 57 57 -
NSIKVAQWMI ADK_HUMAN 57 57 -
NSCRAAQYVL D89173 72 72 -
NSMKVAQWMI ADK_MOUSE 1 1 -
NTARGAAYVL ADK_YEAST 68 68 -

Motif 2 width=15
Element Seqn Id St Int Rpt
FFGCIGIDKFGEILK ADK_CRIGR 75 8 -
FFGCIGIDKFGEILK ADK_MOUSE 19 8 -
FAGCVGQDKFADMLL D89173 88 6 -
FFGCIGIDKFGEILK ADK_HUMAN 75 8 -
FFGCIGIDKFGEILK ADK_RAT 75 8 -
YFGSVGKDKFSERLL ADK_YEAST 84 6 -

Motif 3 width=16
Element Seqn Id St Int Rpt
EQPTGTCAACITGDNR ADK_HUMAN 106 16 -
TTPTGVCAVVLSNNNK D89173 119 16 -
DIGTGKCAALITGHNR ADK_YEAST 115 16 -
EQPTGTCAACITGGNR ADK_MOUSE 50 16 -
EQPTGTCAACISGGNR ADK_RAT 106 16 -
EQPTGTCAACITGDNR ADK_CRIGR 106 16 -

Motif 4 width=27
Element Seqn Id St Int Rpt
WKFVEEAKVIYVGGFHLTVSPESMLCL D89173 159 24 -
WMLVEKARVCYIAGFFLHVSPESVLKV ADK_HUMAN 145 23 -
WVLVEKARVYYIAGFFLTVSPESVLKV ADK_CRIGR 145 23 -
WMLVEKARVYYIAGFFLTVSPESVLKM ADK_RAT 145 23 -
WDLVEAAKLFYIGGFHLTVSPDAIVKL ADK_YEAST 151 20 -
WMLVEKARVYYIAGFFLTVSPESVLKV ADK_MOUSE 89 23 -

Motif 5 width=20
Element Seqn Id St Int Rpt
TQGADATIVAKDGKVTTYKP D89173 268 82 -
THGVEPTVVVSSKGTSTYPV ADK_YEAST 258 80 -
TQGRDDTIVATGNDVTAFPV ADK_RAT 254 82 -
TQGRDDTVVATENEVMAFAV ADK_CRIGR 254 82 -
NQGRDDTIVATGNDVTAFPV ADK_MOUSE 199 83 -
TQGRDDTIMATESEVTAFAV ADK_HUMAN 254 82 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
NSIKVAQWMI O00741 85 85 -
NSIKVAQWMI O00742 68 68 -
NSIKVAQWMI ADK_HUMAN 57 57 -
NSIKVAQWMI ADK_CRIGR 57 57 -
NSMKVAQWMI ADK_RAT 57 57 -
NTIRIAQWML O49923 66 66 -
NSLRVAQWIL Q93934 68 68 -
NTARGAAYVL ADK_YEAST 68 68 -

Motif 2 width=15
Element Seqn Id St Int Rpt
FFGCIGIDKFGEILK O00741 103 8 -
FFGCIGIDKFGEILK O00742 86 8 -
FFGCIGIDKFGEILK ADK_HUMAN 75 8 -
FFGCIGIDKFGEILK ADK_CRIGR 75 8 -
FFGCIGIDKFGEILK ADK_RAT 75 8 -
YFGCVGKDEYGDRMF O49923 83 7 -
FFGAVGKDQYGDLLA Q93934 85 7 -
YFGSVGKDKFSERLL ADK_YEAST 84 6 -

Motif 3 width=16
Element Seqn Id St Int Rpt
EQPTGTCAACITGDNR O00741 134 16 -
EQPTGTCAACITGDNR O00742 117 16 -
EQPTGTCAACITGDNR ADK_HUMAN 106 16 -
EQPTGTCAACITGDNR ADK_CRIGR 106 16 -
EQPTGTCAACISGGNR ADK_RAT 106 16 -
DLPTGTCGVLVVKGER O49923 114 16 -
TVKTGTCAALINGTHR Q93934 116 16 -
DIGTGKCAALITGHNR ADK_YEAST 115 16 -

Motif 4 width=27
Element Seqn Id St Int Rpt
WMLVEKARVCYIAGFFLTVSPESVLKV O00741 173 23 -
WMLVEKARVCYIAGFFLTVSPESVLKV O00742 156 23 -
WMLVEKARVCYIAGFFLHVSPESVLKV ADK_HUMAN 145 23 -
WVLVEKARVYYIAGFFLTVSPESVLKV ADK_CRIGR 145 23 -
WMLVEKARVYYIAGFFLTVSPESVLKM ADK_RAT 145 23 -
WAFVEKAKYIYSAGFFLTVSPESMMTV O49923 152 22 -
QKIIEQAKYFYVTGFFITVCPPAILQL Q93934 154 22 -
WDLVEAAKLFYIGGFHLTVSPDAIVKL ADK_YEAST 151 20 -

Motif 5 width=20
Element Seqn Id St Int Rpt
TQGRDDTIMATESEVTAFAV O00741 282 82 -
TQGRDDTIMATESEVTAFAV O00742 265 82 -
TQGRDDTIMATESEVTAFAV ADK_HUMAN 254 82 -
TQGRDDTVVATENEVMAFAV ADK_CRIGR 254 82 -
TQGRDDTIVATGNDVTAFPV ADK_RAT 254 82 -
TQGTDPTIVAEDGKVTEFPV O49923 261 82 -
TQGPEPVIVVEGDKVTEFPV Q93934 262 81 -
THGVEPTVVVSSKGTSTYPV ADK_YEAST 258 80 -