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PR00978

Identifier
STARPROTEIN  [View Relations]  [View Alignment]  
Accession
PR00978
No. of Motifs
9
Creation Date
14-AUG-1998  (UPDATE 08-JUL-1999)
Title
Steroidogenic acute regulatory protein signature
Database References

INTERPRO; IPR000799
Literature References
1. CLARK B.J., WELLS J., KING S.R. AND STOCCO D.M.
The purification, cloning, and expression of a novel luteinizing hormone-
induced mitochondrial protein in MA-10 mouse Leydig tumor cells.
Characterization of the steroidogenic acute regulatory protein (StAR). 
J.BIOL.CHEM. 269 28314-28322 (1994). 
 
2. GRADI, A., TANG-WAI, R., MCBRIDE, H.M., CHU, L.L., SHORE, G.C. 
AND PELLETIER, J.
The human steroidogenic acute regulatory (StAR) gene is expressed in the
urogenital system and encodes a mitochondrial polypeptide. 
BIOCHIM.BIOPHYS.ACTA. 1258 228-233 (1995). 
 
3. SUGAWARA, T., LIN, D., HOLT, J.A., MARTIN, K.O., JAVITT, N.B.,
MILLER, W.L. AND STRAUSS, J.F. 
Structure of the human steroidogenic acute regulatory protein (STAR) gene: 
STAR stimulates mitochondrial cholesterol 27-hydroxylase activity. 
BIOCHEMISTRY 34 12506-12512 (1995). 

Documentation
Steroidogenic cells respond to trophic hormone stimulation by mobilisation
of cholesterol from cellular stores to the mitochondrial outer membrane, 
and thence to the inner membrane, where the first enzymatic step in 
steroidogenesis occurs [1]. Cholesterol transfer across the membranes is
dependent on de novo protein synthesis, which is the regulated step in
the process [1]. 
 
The newly-synthesised regulatory protein(s) have yet to be identified.
Nevertheless, a candidate mitochondrial protein has been characterised that
is synthesised in response to luteinising hormone stimulation [1]. 
Expression of the protein in the absence of hormone stimulation is 
sufficient to induce steroid production, suggesting that this protein is 
required in the acute regulation of steroidogenesis. This novel protein has
been termed Steroidogenic Acute Regulatory protein (StAR). 
 
STARPROTEIN is a 9-element fingerprint that provides a signature for
steroidogenic acute regulatory proteins. The fingerprint was derived from
an initial alignment of 11 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length. A single iteration
on OWL30.2 was required to reach convergence, no further sequences being
identified beyond the starting set. Two partial matches were found,
SSU72195 and S80098, both of which are fragments that match motifs 3-6.
 
An update on SPTR37_9f identified a true set of 7 sequences, and 2
partial matches.
Summary Information
   7 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
9777777777
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000020020
123456789
True Positives
STAR_BOVIN    STAR_HORSE    STAR_HUMAN    STAR_MESAU    
STAR_MOUSE STAR_PIG STAR_RAT
True Positive Partials
Codes involving 2 elements
Q14849 Q61542
Sequence Titles
STAR_BOVIN  STEROIDOGENIC ACUTE REGULATORY PROTEIN PRECURSOR - BOS TAURUS (BOVINE). 
STAR_HORSE STEROIDOGENIC ACUTE REGULATORY PROTEIN PRECURSOR - EQUUS CABALLUS (HORSE).
STAR_HUMAN STEROIDOGENIC ACUTE REGULATORY PROTEIN PRECURSOR - HOMO SAPIENS (HUMAN).
STAR_MESAU STEROIDOGENIC ACUTE REGULATORY PROTEIN PRECURSOR - MESOCRICETUS AURATUS (GOLDEN HAMSTER).
STAR_MOUSE STEROIDOGENIC ACUTE REGULATORY PROTEIN PRECURSOR (LUTEINIZING HORMONE- INDUCED PROTEIN) - MUS MUSCULUS (MOUSE).
STAR_PIG STEROIDOGENIC ACUTE REGULATORY PROTEIN PRECURSOR - SUS SCROFA (PIG).
STAR_RAT STEROIDOGENIC ACUTE REGULATORY PROTEIN PRECURSOR - RATTUS NORVEGICUS (RAT).

Q14849 MLN64 MRNA - HOMO SAPIENS (HUMAN).
Q61542 ES64 PROTEIN - MUS MUSCULUS (MOUSE).
Scan History
OWL30_2    1  100  NSINGLE    
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
TFKLCAGSSYRHVRSMK STAR_BOVIN 5 5 -
TFKLCAGSSYRHVRNMK STAR_PIG 5 5 -
TFKLCAGSSYRHLRNMK STAR_MESAU 5 5 -
TFKLCAGSSYRHMRNMK STAR_MOUSE 5 5 -
TFKLCAGSSYRHMRNMK STAR_RAT 5 5 -
TFCLCAGSSYRHMRNMK JC5386 83 83 -
TFCLCAGSSYRHMRNMK RNU76419 83 83 -
TFKLCAGSSYRHVRNMK AF031696 5 5 -
TFKLCAGSSYRHVRSMK BTY17259 5 5 -
TFKLCAGSSYRHVRSMK BTY17260 5 5 -
TFKLCAGSSYRHMRNMK STAR_HUMAN 5 5 -

Motif 2 width=20
Element Seqn Id St Int Rpt
GLRHQAVLALGQELNRRALG STAR_PIG 22 0 -
GLRHQAVLAIGQELNRRTLG STAR_MESAU 22 0 -
GLRHQAVLAIGQELNWRALG STAR_MOUSE 22 0 -
GLRHQAVLAIGQELNRRALG STAR_RAT 22 0 -
GLQQQAVLAIGQELNRRALG STAR_BOVIN 22 0 -
GLRHQAALAIGQELNWRAPG AF031696 22 0 -
GLRHQAVLAIGQELNRRALG JC5386 100 0 -
GLRHQAVLAIGQELNRRALG RNU76419 100 0 -
GLQQQAVLAIGQELNRRALG BTY17259 22 0 -
GLQQQAVLAIGQELNRRALG BTY17260 22 0 -
GLRQQAVMAISQELNRRALG STAR_HUMAN 22 0 -

Motif 3 width=20
Element Seqn Id St Int Rpt
LYEELVDRMEAMGEWNPNVK RNU76419 210 90 -
LYEELVERMEAMGEWNPNVK AF031696 133 91 -
LYEELVDRMEAMGEWNPNVK JC5386 210 90 -
LYEELVERMEAMGEWNPNVK BTY17259 133 91 -
LYEELVERMEAMGEWNPNVK BTY17260 133 91 -
LYEELVDRMEAMGEWNPNVK STAR_RAT 132 90 -
LYEELVDRMEAMGEWNPNVK STAR_MOUSE 132 90 -
LYAELVDRMEAMGEWNPNVK STAR_MESAU 132 90 -
LYEELVERMEAMGEWNPSVK STAR_PIG 133 91 -
LYEELVERMEAMGEWNPNVK STAR_HUMAN 133 91 -
LYEELVERMEAMGEWNPNVK STAR_BOVIN 133 91 -

Motif 4 width=18
Element Seqn Id St Int Rpt
KKIKILQKIGKDTVITHE STAR_PIG 152 -1 -
KEIKVLQKIGKDTFITHE STAR_HUMAN 152 -1 -
KEIKVLQKIGKDTVITHE BTY17260 152 -1 -
KEIKVLQKIGKDTVITHE BTY17259 152 -1 -
KEIKVLKKIGKDTVITHE RNU76419 229 -1 -
KEIKVLKKIGKDTVITHE JC5386 229 -1 -
KEIKVLQKIGKDTVITHE AF031696 152 -1 -
KEIKVLQKIGKDTVITHE STAR_BOVIN 152 -1 -
KEIKVLKKIGKDTVITHE STAR_RAT 151 -1 -
KEIKVLQRIGKDTVITHE STAR_MOUSE 151 -1 -
KEIKVLQKIGKDTVITHE STAR_MESAU 151 -1 -

Motif 5 width=21
Element Seqn Id St Int Rpt
ELAAEVAGNLVGPRDFVSVRC STAR_BOVIN 169 -1 -
ELAAEAAGNLVGPRDFVSVRC STAR_HUMAN 169 -1 -
ELAAEAAGNLVGPRDFVSVRC BTY17260 169 -1 -
ELAAEAAGNLVGPRDFVSVRC BTY17259 169 -1 -
ELAAAAAGNLVGPRDFVSVRC RNU76419 246 -1 -
ELAAAARGNLVGPRDFVSVRC JC5386 246 -1 -
ELAAESAGNLVGPRDFVSVRC AF031696 169 -1 -
ELAAAAAGNLVGPRDFVSVRC STAR_RAT 168 -1 -
ELAAEAAGNLVGPRDFVSVGC STAR_PIG 169 -1 -
ELAAAAAGNLVGPRDFVSVRC STAR_MESAU 168 -1 -
ELAAAAAGNLVGPRDFVSVRC STAR_MOUSE 168 -1 -

Motif 6 width=19
Element Seqn Id St Int Rpt
KRRGSTCVLAGMATHFGEM STAR_MOUSE 190 1 -
KRRGSTCVLAGMATHFGEM STAR_RAT 190 1 -
KRRGSTCVLAGMATHFGEM JC5386 268 1 -
KRRGSTCVLAGMATQFEEM AF031696 191 1 -
KRRGSMCVLAGMATLYEEM BTY17260 191 1 -
KRRGSMCVLAGMATLYEEM BTY17259 191 1 -
KRRGSTCVLAGMATHFGEM RNU76419 268 1 -
KRRGSMCVLAGMATLYEEM STAR_BOVIN 191 1 -
KRRGSTCVLAGMDTDFGNM STAR_HUMAN 191 1 -
KRRGSVCVLAGMATDFGEM STAR_PIG 191 1 -
KRRGSTCVLAGIATHFGEM STAR_MESAU 190 1 -

Motif 7 width=20
Element Seqn Id St Int Rpt
PTCMVLHPLAGSPSKTKLTW STAR_MOUSE 221 12 -
PTCMVLRPLAGSPSRTKLTW BTY17260 222 12 -
PTCMVLHPLAGSPSKTKLTW STAR_RAT 221 12 -
PTCMVLRPLAGSPSRTKLTW BTY17259 222 12 -
PTCMVLHPLAGSPSKTKFTW STAR_MESAU 221 12 -
PTCMVLHPLAGSPSKTKLTW STAR_PIG 222 12 -
PTCMVLHPLAGSPSKTKLTW STAR_HUMAN 222 12 -
PTCMVLRPLAGSPSRTKLTW STAR_BOVIN 222 12 -
PTCMVLHPLAGSPSKTKLTW AF031696 222 12 -
PTCMVLHPLAGSPSKTKLTW JC5386 299 12 -
PTCMVLHPLAGSPSKTKLTW RNU76419 299 12 -

Motif 8 width=20
Element Seqn Id St Int Rpt
WLLSIDLKGWLPKTIINQVL STAR_MOUSE 240 -1 -
WLLSIDLKGWLPKSIINQVL STAR_MESAU 240 -1 -
WLLSIDLKGWLPKTIINQVL BTY17260 241 -1 -
WLLSIDLKGWLPKTIINQVL BTY17259 241 -1 -
WLLSIDLKGWLPKTIINQVL STAR_PIG 241 -1 -
WLLSIDLKGWLPKSIINQVL STAR_HUMAN 241 -1 -
WLLSIDLKGWLPKTIINQVL STAR_BOVIN 241 -1 -
WLLSIDLKGWLPKTIINQVL RNU76419 318 -1 -
WLLSIDLKVWLPKTIINQVL JC5386 318 -1 -
WLLSIDLKGWLPKTIINQVL AF031696 241 -1 -
WLLSIDLKGWLPKTIINQVL STAR_RAT 240 -1 -

Motif 9 width=23
Element Seqn Id St Int Rpt
SQTQIEFASHLRKRLESSPASEA RNU76419 338 0 -
SQTQVDFANHLRKRLESSPAPEA AF031696 261 0 -
SQTQVDFANHLRKRLESCPALEA STAR_BOVIN 261 0 -
SQTQVDFANHLRKRLESHPASEA STAR_HUMAN 261 0 -
SQTQVDFANHLRKRLESRPALEA STAR_PIG 261 0 -
SQTQMEFANHLRKRLESSSASEA STAR_MESAU 260 0 -
SQTQIEFANHLRKRLEASPASEA STAR_MOUSE 260 0 -
SQTQVDFANHLRKRLESCPALEA BTY17260 261 0 -
SQTQIEFASHLRKRLESSPASEA STAR_RAT 260 0 -
SQTQVDFANHLRKRLESCPALEA BTY17259 261 0 -
SQTQIEFASHLRKRLESSPASEA JC5386 338 0 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
TFKLCAGSSYRHVRSMK STAR_BOVIN 5 5 -
TFKLCAGSSYRHMRNMK STAR_MOUSE 5 5 -
TFKLCAGSSYRHVRNMK STAR_HORSE 5 5 -
TFKLCAGSSYRHMRNMK STAR_RAT 5 5 -
TFKLCAGSSYRHLRNMK STAR_MESAU 5 5 -
TFKLCAGSSYRHVRNMK STAR_PIG 5 5 -
TFKLCAGSSYRHMRNMK STAR_HUMAN 5 5 -

Motif 2 width=20
Element Seqn Id St Int Rpt
GLQQQAVLAIGQELNRRALG STAR_BOVIN 22 0 -
GLRHQAVLAIGQELNRRALG STAR_RAT 22 0 -
GLRHQAVLAIGQELNWRALG STAR_MOUSE 22 0 -
GLRHQAALAIGQELNWRAPG STAR_HORSE 22 0 -
GLRHQAVLAIGQELNRRTLG STAR_MESAU 22 0 -
GLRHQAVLALGQELNRRALG STAR_PIG 22 0 -
GLRQQAVMAISQELNRRALG STAR_HUMAN 22 0 -

Motif 3 width=20
Element Seqn Id St Int Rpt
LYEELVDRMEAMGEWNPNVK STAR_RAT 132 90 -
LYEELVDRMEAMGEWNPNVK STAR_MOUSE 132 90 -
LYEELVERMEAMGEWNPNVK STAR_HORSE 133 91 -
LYAELVDRMEAMGEWNPNVK STAR_MESAU 132 90 -
LYEELVERMEAMGEWNPSVK STAR_PIG 133 91 -
LYEELVERMEAMGEWNPNVK STAR_HUMAN 133 91 -
LYEELVERMEAMGEWNPNVK STAR_BOVIN 133 91 -

Motif 4 width=18
Element Seqn Id St Int Rpt
KEIKVLKKIGKDTVITHE STAR_RAT 151 -1 -
KEIKVLQRIGKDTVITHE STAR_MOUSE 151 -1 -
KEIKVLQKIGKDTVITHE STAR_HORSE 152 -1 -
KEIKVLQKIGKDTVITHE STAR_MESAU 151 -1 -
KKIKILQKIGKDTVITHE STAR_PIG 152 -1 -
KEIKVLQKIGKDTFITHE STAR_HUMAN 152 -1 -
KEIKVLQKIGKDTVITHE STAR_BOVIN 152 -1 -

Motif 5 width=21
Element Seqn Id St Int Rpt
ELAAAAAGNLVGPRDFVSVRC STAR_MESAU 168 -1 -
ELAAAAAGNLVGPRDFVSVRC STAR_RAT 168 -1 -
ELAAAAAGNLVGPRDFVSVRC STAR_MOUSE 168 -1 -
ELAAESAGNLVGPRDFVSVRC STAR_HORSE 169 -1 -
ELAAEAAGNLVGPRDFVSVGC STAR_PIG 169 -1 -
ELAAEAAGNLVGPRDFVSVRC STAR_HUMAN 169 -1 -
ELAAEVAGNLVGPRDFVSVRC STAR_BOVIN 169 -1 -

Motif 6 width=19
Element Seqn Id St Int Rpt
KRRGSTCVLAGMATHFGEM STAR_RAT 190 1 -
KRRGSTCVLAGMATHFGEM STAR_MOUSE 190 1 -
KRRGSTCVLAGMATQFEEM STAR_HORSE 191 1 -
KRRGSTCVLAGIATHFGEM STAR_MESAU 190 1 -
KRRGSVCVLAGMATDFGEM STAR_PIG 191 1 -
KRRGSTCVLAGMDTDFGNM STAR_HUMAN 191 1 -
KRRGSMCVLAGMATLYEEM STAR_BOVIN 191 1 -

Motif 7 width=20
Element Seqn Id St Int Rpt
PTCMVLHPLAGSPSKTKLTW STAR_RAT 221 12 -
PTCMVLHPLAGSPSKTKLTW STAR_MOUSE 221 12 -
PTCMVLHPLAGSPSKTKLTW STAR_HORSE 222 12 -
PTCMVLHPLAGSPSKTKFTW STAR_MESAU 221 12 -
PTCMVLHPLAGSPSKTKLTW STAR_PIG 222 12 -
PTCMVLHPLAGSPSKTKLTW STAR_HUMAN 222 12 -
PTCMVLRPLAGSPSRTKLTW STAR_BOVIN 222 12 -

Motif 8 width=20
Element Seqn Id St Int Rpt
WLLSIDLKGWLPKTIINQVL STAR_RAT 240 -1 -
WLLSIDLKGWLPKTIINQVL STAR_MOUSE 240 -1 -
WLLSIDLKGWLPKTIINQVL STAR_HORSE 241 -1 -
WLLSIDLKGWLPKSIINQVL STAR_MESAU 240 -1 -
WLLSIDLKGWLPKTIINQVL STAR_PIG 241 -1 -
WLLSIDLKGWLPKSIINQVL STAR_HUMAN 241 -1 -
WLLSIDLKGWLPKTIINQVL STAR_BOVIN 241 -1 -

Motif 9 width=23
Element Seqn Id St Int Rpt
SQTQIEFASHLRKRLESSPASEA STAR_RAT 260 0 -
SQTQIEFANHLRKRLEASPASEA STAR_MOUSE 260 0 -
SQTQVDFANHLRKRLESSPAPEA STAR_HORSE 261 0 -
SQTQMEFANHLRKRLESSSASEA STAR_MESAU 260 0 -
SQTQVDFANHLRKRLESRPALEA STAR_PIG 261 0 -
SQTQVDFANHLRKRLESHPASEA STAR_HUMAN 261 0 -
SQTQVDFANHLRKRLESCPALEA STAR_BOVIN 261 0 -