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PR00977

Identifier
SCYTLDPTASE  [View Relations]  [View Alignment]  
Accession
PR00977
No. of Motifs
5
Creation Date
10-SEP-1998  (UPDATE 07-JUN-1999)
Title
Scytalidopepsin B aspartic protease (A4) signature
Database References

INTERPRO; IPR000250
Literature References
1. RAWLINGS, N.D. AND BARRETT A.J.
Families of aspartic peptidases, and those of unkown catalytic mechanism.
METHODS ENZYMOL. 248 183-228 (1995).
 
2. RAWLINGS, N.D. AND BARRETT, A.J.
Evolutionary families of peptidases.
BIOCHEM.J. 290 205-218 (1993).
 
3. RAWLINGS, N.D. AND BARRETT, A.J.
MEROPS - Peptidase Database
http://www.bi.bbsrc.ac.uk/merops/merops.htm
 
4. RAWLINGS, N.D. AND BARRETT, A.J.
Family A4 - Scytalidopepsin B aspartic peptidases
http://www.bi.bbsrc.ac.uk/merops/famcards/a4.htm
 
5. INOUE, H., KIMURA, T., MAKABE, O. AND TAKAHASHI, K.
The gene and deduced protein sequences of the zymogen of Aspergillus niger
acid proteinase A. 
J.BIOL.CHEM. 266 19484-19489 (1991).

Documentation
Aspartic proteases have a catalytic aspartate residue at their active site.
A number of families (denoted by the letter A followed by a number) have 
been identified, most of which (including pepsin, retropepsin and 
cauliflower mosaic virus peptidase) have been grouped into a single clan 
(AA), as they are all thought to share a common ancestry. Families A6 and
A21 belong to the AB clan. The remaining families have not yet received 
clan assignments [1-3]. 
 
Scytalidopepsin B is a fungal aspartic protease belonging to the A4 family
[1,4]. This family of proteases has not yet been assigned to a clan. The
proteins are thermostable, pepstatin insensitive and are active at low pH 
ranges [1]. The enzyme has a unique heterodimeric structure, with a
39-residue light chain and a 173-residue heavy chain bound to each other
non-covalently [5]. 
 
SCYTLDPTASE is a 5-element fingerprint that provides a signature for the
scytalidopepsin B aspartic protease (A4) family. The fingerprint was 
derived from an initial alignment of 4 sequences: the motifs were drawn 
from conserved regions spanning virtually the full length of the heavy
chain. A single iteration on OWL30.2 was required to reach convergence
at which point a true set comprising 5 sequences was identified. 
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
544444
400000
300000
200000
12345
True Positives
PRTA_ASPNG    PRTB_SCYLI    Q00550        Q00551        
Sequence Titles
PRTA_ASPNG  ASPERGILLOPEPSIN II PRECURSOR (EC 3.4.23.19) (ACID PROTEASE A) (PROCTASE A) - ASPERGILLUS NIGER. 
PRTB_SCYLI SCYTALIDOPEPSIN B (EC 3.4.23.32) (ACID PROTEASE B) (SLB) - SCYTALIDIUM LIGNICOLUM.
Q00550 ACID PROTEINASE - CRYPHONECTRIA PARASITICA (CHESNUT BLIGHT FUNGUS) (ENDOTHIA PARASITICA).
Q00551 ACID PROTEINASE - CRYPHONECTRIA PARASITICA (CHESNUT BLIGHT FUNGUS) (ENDOTHIA PARASITICA).
Scan History
OWL30_2    1  50   NSINGLE    
SPTR37_9f 2 5 NSINGLE
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
ASAWVGIDGDTAQNSILQTGVDF S63631 104 104 -
ASAWVGIDGDTCTSAILQTGVDF S63630 108 108 -
ASAWVGIDGDTCETAILQTGVDF PRTA_ASPNG 116 116 -
SAAWVGIDGDTCQTAILQTGFDW PRTB_SCYLI 36 36 -

Motif 2 width=21
Element Seqn Id St Int Rpt
FDAWYEWYPNYAYDFSGFSVS S63630 139 8 -
YDAWYEWYPDYAYDFSDITIS PRTA_ASPNG 147 8 -
YDAWYEWYPEVSDDFSGITIS PRTB_SCYLI 64 5 -
YDAWYEWYPDYAYDFSGISIS S63631 135 8 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GDTIVMTASASSLKAGTV S63630 161 1 -
GDTIKVTVTATTTTSGTA S63631 157 1 -
GDSIQMSVTATSDTSGSA PRTB_SCYLI 86 1 -
GDSIKVTVEATSKSSGSA PRTA_ASPNG 169 1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
ENSTTGKKVTQSFS S63630 181 2 -
ENLTTGQKVSKSFS PRTB_SCYLI 106 2 -
ENLTTGQSVTHTFS PRTA_ASPNG 189 2 -
ENVTKGTTVTHTFT S63631 177 2 -

Motif 5 width=16
Element Seqn Id St Int Rpt
LQELNAEWIVEDFEEG S63631 196 5 -
LCEYNAEWIVEDFESG S63630 200 5 -
LCRTNAEFIIEDFEEC PRTB_SCYLI 125 5 -
LCETNAEWIVEDFESG PRTA_ASPNG 209 6 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
ASAWVGIDGDTCETAILQTGVDF PRTA_ASPNG 116 116 -
ASAWVGIDGDTAQNSILQTGVDF Q00551 104 104 -
ASAWVGIDGDTCTSAILQTGVDF Q00550 108 108 -
SAAWVGIDGDTCQTAILQTGFDW PRTB_SCYLI 36 36 -

Motif 2 width=21
Element Seqn Id St Int Rpt
YDAWYEWYPDYAYDFSDITIS PRTA_ASPNG 147 8 -
YDAWYEWYPDYAYDFSGISIS Q00551 135 8 -
FDAWYEWYPNYAYDFSGFSVS Q00550 139 8 -
YDAWYEWYPEVSDDFSGITIS PRTB_SCYLI 64 5 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GDSIKVTVEATSKSSGSA PRTA_ASPNG 169 1 -
GDTIKVTVTATTTTSGTA Q00551 157 1 -
GDTIVMTASASSLKAGTV Q00550 161 1 -
GDSIQMSVTATSDTSGSA PRTB_SCYLI 86 1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
ENLTTGQSVTHTFS PRTA_ASPNG 189 2 -
ENVTKGTTVTHTFT Q00551 177 2 -
ENSTTGKKVTQSFS Q00550 181 2 -
ENLTTGQKVSKSFS PRTB_SCYLI 106 2 -

Motif 5 width=16
Element Seqn Id St Int Rpt
LCETNAEWIVEDFESG PRTA_ASPNG 209 6 -
LQELNAEWIVEDFEEG Q00551 196 5 -
LCEYNAEWIVEDFESG Q00550 200 5 -
LCRTNAEFIIEDFEEC PRTB_SCYLI 125 5 -