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PR00957

Identifier
GENE66  [View Relations]  [View Alignment]  
Accession
PR00957
No. of Motifs
8
Creation Date
14-AUG-1998  (UPDATE 10-JUN-1999)
Title
Gene 66 (IR5) protein signature
Database References

INTERPRO; IPR000714
Literature References
1. HOLDEN V.R., YALAMANCHILI R.R., HARTY R.N., O'CALLAGHAN D.J.
Identification and characterization of an equine herpesvirus 1 late gene
encoding a potential zinc finger. 
VIROLOGY 188 704-713 (1992).
 
2. SAKAGUCHI, M., URAKAWA, T., HIRAYAMA, Y., MIKI, N., YAMAMOTO, M.
AND HIRAI, K.
Sequence determination and genetic content of an 8.9Kb restriction fragment
in the short unique region and the internal inverted repeat of Marek's 
disease virus type 1 DNA.
VIRUS GENES 6 365-378 (1992). 

Documentation
The IR5 open reading frame (ORF) of the equine herpesvirus type 1 (EHV-1)
genome maps within the inverted repeat segments [1]. Sequence analyses of
the gene region revealed an ORF of 236 amino acids that showed a high degree
of similarity to ORF64 of varicella zoster virus and ORF3 of EHV-4, both of
which map within the inverted repeats, and to the US10 ORF of herpes simplex
virus type 1 (HSV-1), which maps within the unique short segment [1]. 
 
The IR5 ORF houses a sequence of 13 residues (CAYWCCLGHAFAC) that matches
perfectly the consensus zinc finger motif (C-X2-4-C-X2-15-C/H-X2-4-C/H) [1].
Putative cis-acting elements flanking the IR5 ORF include a TATA box, a 
CAAT box, and a polyadenylation signal. Coupled with various experimental
data, the IR5 gene of EHV-1 thus exhibits characteristics representative of
a late gene of the gamma-1 class. 
 
The DNA sequence covering ~70% of the short unique region (Us) and part of 
the short inverted repeat of the Marek's disease virus type 1 GA strain has
been determined [2]. Sequence analysis showed the presence of nine potential
ORFs in the Us region, four of which were found to be similar to US10 
(minor virion protein) [2].
 
GENE66 is an 8-element fingerprint that provides a signature for so-called
gene 66 (IR5) proteins. The fingerprint was derived from an initial 
alignment of 4 sequences: the motifs were drawn from short conserved 
regions spanning virtually the full alignment length - motif 4 includes the
first 10 residues of the putative zinc-finger motif. A single iteration on
OWL30.2 was required to reach convergence, no further sequences being 
identified beyond the starting set. A single partial match was found, 
U639_HSVMG, a hypothetical Marek's disease herpesvirus protein that matches
motifs 3 and 4. 
 
An update on SPTR37_9f identified a true set of 5 sequences, and 2
partial matches.
Summary Information
   5 codes involving  8 elements
1 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
855555555
711111110
600000000
500000000
400000000
300000000
200110000
12345678
True Positives
O42059        Q66680        US10_HSVE4    US10_HSVEB    
US10_HSVEK
True Positive Partials
Codes involving 7 elements
Q69361
Codes involving 2 elements
U639_HSVMG
Sequence Titles
O42059      COUNTERPART OF HSV-1 GENE US10 AND VZV GENE 64 - EQUINE HERPESVIRUS 4. 
Q66680 VIRION PROTEIN US10 - EQUINE HERPESVIRUS 1.
US10_HSVE4 28 KD PROTEIN (ORF3) - EQUINE HERPESVIRUS TYPE 4 (STRAIN 1942) (EHV-4) (EQUINE HERPESVIRUS TYPE 1 SUBTYPE 2).
US10_HSVEB GENE 66 PROTEIN - EQUINE HERPESVIRUS TYPE 1 (STRAIN AB4P) (EHV-1).
US10_HSVEK GENE 66 PROTEIN (IR5 PROTEIN) (ORF S2-1) - EQUINE HERPESVIRUS TYPE 1 (STRAIN KENTUCKY A) (EHV-1).

Q69361 HOMOLOGOUS TO PROTEIN ENCODED BY HSV-1 US10 - FELINE HERPESVIRUS (FELID HERPESVIRUS 1).

U639_HSVMG HYPOTHETICAL 23.6 KD PROTEIN - MAREK'S DISEASE HERPESVIRUS (STRAIN GA) (MDHV).
Scan History
OWL30_2    1  50   NSINGLE    
SPTR37_9f 2 47 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
YPTSTDTAAHAVSLPRSV US10_HSVEB 35 35 -
YPTSTDTAAHAVSLPRSV US10_HSVEK 35 35 -
YPLRGDSADHAETLPRSV A370054 24 24 -
YPTSTDTAAHAVSLPRSV US10_HSVE4 59 59 -

Motif 2 width=27
Element Seqn Id St Int Rpt
RAMSADAADALRRGAGPPPEIWPRAYR US10_HSVE4 84 7 -
RAVSAEAADALRSGAGPPAEAWPRVYR US10_HSVEK 60 7 -
RVASCEAFCLMRLGGPPPADIWPGVYR A370054 49 7 -
RAVSAEAADALRSGAGPPAEAWPRVYR US10_HSVEB 60 7 -

Motif 3 width=21
Element Seqn Id St Int Rpt
FHSADPLRRAVGRYLVDLGAA US10_HSVE4 127 16 -
FHVADPIRHLVGRYLMGLGPA A370054 96 20 -
FHSADPLRRAVGRYLVDLGAA US10_HSVEB 103 16 -
FHSADPLRRAVGLYLVDLGAA US10_HSVEK 103 16 -

Motif 4 width=22
Element Seqn Id St Int Rpt
ETHAELSTRLLFCAHWCCLGHA US10_HSVE4 150 2 -
ESHPELHTRLLYCAYWCCLGHA A370054 119 2 -
ETHAELSGRMLFCAYWCCLGHA US10_HSVEB 126 2 -
ETHAELSGRMLFCAYWCCLGHA US10_HSVEK 126 2 -

Motif 5 width=16
Element Seqn Id St Int Rpt
CSRPQMYERACARFFE US10_HSVEB 150 2 -
CTHSHIYEDACRRFFE A370054 143 2 -
CSRQAMYERECARFFE US10_HSVE4 174 2 -
CSRPQMYERACARFFE US10_HSVEK 150 2 -

Motif 6 width=17
Element Seqn Id St Int Rpt
GAGEIPPADAVAHWNAL A370054 162 3 -
GIGETPPADAERYWAAL US10_HSVEB 169 3 -
GIGETPPADAERYWAAL US10_HSVEK 169 3 -
GIGETPPADSERYWVAL US10_HSVE4 193 3 -

Motif 7 width=19
Element Seqn Id St Int Rpt
MAGADPELFPRHAAAAAYL US10_HSVE4 212 2 -
MVLDEPELLVKHAAAAVYL A370054 181 2 -
MAGAEPELFPRHAAAAAYL US10_HSVEB 188 2 -
MAGAEPELFPRHAAAAAYL US10_HSVEK 188 2 -

Motif 8 width=11
Element Seqn Id St Int Rpt
GRKLPLQLPSA US10_HSVEB 210 3 -
GRKLPLPLPPQ US10_HSVE4 234 3 -
GRKLPLQLPSA US10_HSVEK 210 3 -
RRNYGGCIPNI A370054 201 1 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
YPTSTDTAAHAVSLPRSV Q66680 35 35 -
YPTSTDTAAHAVSLPRSV US10_HSVEB 35 35 -
YPTSTDTAAHAVSLPRSV US10_HSVEK 35 35 -
YPTSTDTAAHAVSLPRSV O42059 28 28 -
YPTSTDTAAHAVSLPRSV US10_HSVE4 59 59 -

Motif 2 width=27
Element Seqn Id St Int Rpt
RAVSAEAADALRSGAGPPAEAWPRVYR Q66680 60 7 -
RAVSAEAADALRSGAGPPAEAWPRVYR US10_HSVEB 60 7 -
RAVSAEAADALRSGAGPPAEAWPRVYR US10_HSVEK 60 7 -
RAMSADAADALRRGAGPPPEIWPRAYR O42059 53 7 -
RAMSADAADALRRGAGPPPEIWPRAYR US10_HSVE4 84 7 -

Motif 3 width=21
Element Seqn Id St Int Rpt
FHSADPLRRAVGRYLVDLGAA Q66680 103 16 -
FHSADPLRRAVGRYLVDLGAA US10_HSVEB 103 16 -
FHSADPLRRAVGLYLVDLGAA US10_HSVEK 103 16 -
FHSADPLRRAVGRYLVDLGAA O42059 96 16 -
FHSADPLRRAVGRYLVDLGAA US10_HSVE4 127 16 -

Motif 4 width=22
Element Seqn Id St Int Rpt
ETHAELSGRMLFCAYWCCLGHA Q66680 126 2 -
ETHAELSGRMLFCAYWCCLGHA US10_HSVEB 126 2 -
ETHAELSGRMLFCAYWCCLGHA US10_HSVEK 126 2 -
ETHAELSTRLLFCAHWCCLGHA O42059 119 2 -
ETHAELSTRLLFCAHWCCLGHA US10_HSVE4 150 2 -

Motif 5 width=16
Element Seqn Id St Int Rpt
CSRPQMYERACARFFE Q66680 150 2 -
CSRPQMYERACARFFE US10_HSVEB 150 2 -
CSRPQMYERACARFFE US10_HSVEK 150 2 -
CSRQAMYERECARFFE O42059 143 2 -
CSRQAMYERECARFFE US10_HSVE4 174 2 -

Motif 6 width=17
Element Seqn Id St Int Rpt
GIGETPPADAERYWAAL Q66680 169 3 -
GIGETPPADAERYWAAL US10_HSVEB 169 3 -
GIGETPPADAERYWAAL US10_HSVEK 169 3 -
GIGETPPADSERYWVAL O42059 162 3 -
GIGETPPADSERYWVAL US10_HSVE4 193 3 -

Motif 7 width=19
Element Seqn Id St Int Rpt
MAGAEPELFPRHAAAAAYL Q66680 188 2 -
MAGAEPELFPRHAAAAAYL US10_HSVEB 188 2 -
MAGAEPELFPRHAAAAAYL US10_HSVEK 188 2 -
MAGADPELFPRHAAAAAYL O42059 181 2 -
MAGADPELFPRHAAAAAYL US10_HSVE4 212 2 -

Motif 8 width=11
Element Seqn Id St Int Rpt
GRKLPLQLPSA Q66680 210 3 -
GRKLPLQLPSA US10_HSVEB 210 3 -
GRKLPLQLPSA US10_HSVEK 210 3 -
GRKLPLPLPPQ O42059 203 3 -
GRKLPLPLPPQ US10_HSVE4 234 3 -