SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00924

Identifier
ALKEXNUCLASE  [View Relations]  [View Alignment]  
Accession
PR00924
No. of Motifs
6
Creation Date
26-JUN-1998  (UPDATE 06-JUN-1999)
Title
Alphaherpesvirus alkaline exonuclease signature
Database References

INTERPRO; IPR001616
Literature References
1. TELFORD, E.A., WATSON, M.S., MCBRIDE, K. AND DAVISON, A.J.
The DNA sequence of equine herpesvirus-1. 
VIROLOGY 189 304-316 (1992). 
 
2. DRAPER, K.G., DEVI-RAO, G., COSTA, R.H., BLAIR, E.D., THOMPSON, R.L.
AND WAGNER, E.K.
Characterization of the genes encoding herpes simplex virus type 1 and 
type 2 alkaline exonucleases and overlapping proteins. 
J.VIROL. 57 1023-1036 (1986).

Documentation
Equine herpesvirus-1 is a respiratory virus capable of causing abortion and
neurological disease. Its complete DNA sequence has been determined [1] and
the constituent genes found to be arranged co-linearly with those in the
genomes of other alphaherpesviruses, namely varicella-zoster virus and 
herpes simplex virus type-1 (HSV-1) [1]. Comparisons of the predicted amino
acid sequences have allowed functions of many equine herpesvirus 1 proteins
to be inferred. 
 
For example, detailed analysis of HSV-1 and HSV-2 DNA has revealed an open 
reading frame sufficient to encode 626 amino acids for the HSV-1 alkaline
exonuclease (620 amino acids for HSV-2) [2]. Comparison of the predicted
amino acid sequences of the viral enzymes has revealed significant
differences in the N-terminal portions of the proteins; nevertheless, their
three-dimensional structures are believed to be similar. 
 
ALKEXNUCLASE is a 6-element fingerprint that provides a signature for the
alphaherpesvirus alkaline exonuclease. The fingerprint was derived from an 
initial alignment of 5 sequences: the motifs were drawn from conserved
regions spanning the C-terminal half of the alignment. Three iterations
on OWL30.2 were required to reach convergence, at which point a true set
comprising 19 sequences was identified. A single partial match was also
found, JC5169, a pseudorabies virus alkaline nuclease that matches motifs
1,4,5 and 6.
 
An update on SPTR37_9f identified a true set of 21 sequences, and 1
partial match.
Summary Information
  21 codes involving  6 elements
0 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6212121212121
5000000
4100111
3000000
2000000
123456
True Positives
EXON_EBV      EXON_HCMVA    EXON_HSV11    EXON_HSV2     
EXON_HSV6U EXON_HSV6Z EXON_HSV7J EXON_HSVEB
EXON_HSVSA EXON_VZVD O36386 O39290
O40930 O41954 P88925 P89435
Q65572 Q66641 Q68978 Q68979
Q85039
True Positive Partials
Codes involving 4 elements
Q87066
Sequence Titles
EXON_EBV    ALKALINE EXONUCLEASE (EC 3.1.11.-) - EPSTEIN-BARR VIRUS (STRAIN B95-8) (HUMAN HERPESVIRUS 4). 
EXON_HCMVA ALKALINE EXONUCLEASE (EC 3.1.11.-) - HUMAN CYTOMEGALOVIRUS (STRAIN AD169).
EXON_HSV11 ALKALINE EXONUCLEASE (EC 3.1.11.-) - HERPES SIMPLEX VIRUS (TYPE 1 / STRAIN 17).
EXON_HSV2 ALKALINE EXONUCLEASE (EC 3.1.11.-) - HERPES SIMPLEX VIRUS (TYPE 2).
EXON_HSV6U ALKALINE EXONUCLEASE (EC 3.1.11.-) - HERPES SIMPLEX VIRUS (TYPE 6 / STRAIN UGANDA-1102).
EXON_HSV6Z ALKALINE EXONUCLEASE (EC 3.1.11.-) - HERPES SIMPLEX VIRUS (TYPE 6 / STRAIN Z29).
EXON_HSV7J ALKALINE EXONUCLEASE (EC 3.1.11.-) - HERPES SIMPLEX VIRUS (TYPE 7 / STRAIN JI) (HHV7).
EXON_HSVEB ALKALINE EXONUCLEASE (EC 3.1.11.-) - EQUINE HERPESVIRUS TYPE 1 (STRAIN AB4P) (EHV-1).
EXON_HSVSA ALKALINE EXONUCLEASE (EC 3.1.11.-) - HERPESVIRUS SAIMIRI (STRAIN 11).
EXON_VZVD ALKALINE EXONUCLEASE (EC 3.1.11.-) - VARICELLA-ZOSTER VIRUS (STRAIN DUMAS) (VZV).
O36386 ALKALINE EXONUCLEASE - ALCELAPHINE HERPESVIRUS 1 (WILDEBEEST HERPESVIRUS).
O39290 COUNTERPART OF HSV-1 GENE UL12 AND VZV GENE 48 - EQUINE HERPESVIRUS 4.
O40930 ORF 37 - KAPOSI'S SARCOMA-ASSOCIATED HERPESVIRUS (KSHV) (HUMAN HERPESVIRUS 8).
O41954 ALKALINE EXONUCLEASE - MURINE HERPESVIRUS 68.
P88925 ORF 37 - KAPOSI'S SARCOMA-ASSOCIATED HERPESVIRUS (KSHV) (HUMAN HERPESVIRUS 8).
P89435 DEOXYRIBONUCLEASE - HERPES SIMPLEX VIRUS (TYPE 2).
Q65572 DEOXYRIBONUCLEASE - BOVINE HERPESVIRUS 1.
Q66641 DEOXYRIBONUCLEASE - EQUINE HERPESVIRUS 2.
Q68978 ALKALINE EXONUCLEASE - HUMAN HERPESVIRUS 1.
Q68979 1.9KD ORF - HUMAN HERPESVIRUS 1.
Q85039 UL12 - PSEUDORABIES VIRUS.

Q87066 ALKALINE NUCLEASE - PSEUDORABIES VIRUS.
Scan History
OWL30_2    3  100  NSINGLE    
SPTR37_9f 2 26 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
EAATRAQSESDLWTLLRR PRVULGNS1 91 91 -
EAATQNQADCQLWALLRR EXON_HSV11 219 219 -
EAATQNQADCQLWALLRR EXON_HSV2 209 209 -
EAQTRDQSESQLWALLRR EXON_HSVEB 111 111 -
ESETRGQGDNAIWTLLRR EXON_VZVD 127 127 -

Motif 2 width=24
Element Seqn Id St Int Rpt
CGLLMDARTGVVGASLDMLVCDRD EXON_HSVEB 219 90 -
CGVLMDGHTGMVGASLDILVCPRD EXON_HSV2 314 87 -
CGLLLDPRTGMVGASLDLLVCDRD PRVULGNS1 207 98 -
CGLLIDVRTGLIGASLDVLVCDRD EXON_VZVD 244 99 -
CGVLMDGHTGMVGASLDILVCPRD EXON_HSV11 324 87 -

Motif 3 width=18
Element Seqn Id St Int Rpt
TPLAFYEVKCRAKYAFDP EXON_HSV2 348 10 -
TEMRFFEIKCRAKYLFSA PRVULGNS1 242 11 -
TPLAFYEVKCRAKYAFDP EXON_HSV11 358 10 -
TTLDFFEIKCRAKYLFDP EXON_HSVEB 254 11 -
TDISFFEIKCRAKYLFDP EXON_VZVD 280 12 -

Motif 4 width=17
Element Seqn Id St Int Rpt
RKFLRSIKNPAVEYFAP EXON_HSVEB 294 22 -
RGFLYSIARPGVEFFEG PRVULGNS1 282 22 -
RDFLYTIKNPCVSFFGP EXON_VZVD 321 23 -
RAFIRSIPKPSVRYFAP EXON_HSV11 399 23 -
RAFIRSIPNPGVRYFAP EXON_HSV2 389 23 -

Motif 5 width=21
Element Seqn Id St Int Rpt
PVFANPRHPNFKQILVQGYVL EXON_HSV11 490 74 -
PIFANPRHPNFKQILVQGYVL EXON_HSV2 480 74 -
PVFANPRHPNFKQILVQAYVL EXON_HSVEB 385 74 -
PVFANPRHANFKQIAVQTYVL EXON_VZVD 411 73 -
PVFANPRHANFRQILVQSYVV PRVULGNS1 372 73 -

Motif 6 width=19
Element Seqn Id St Int Rpt
ILPDQAVPIALIITPVRVE EXON_HSV2 553 52 -
IPPHCAVPVIAVLTPIEVD EXON_VZVD 478 46 -
VHSSCAIPILLFVTPVIVD EXON_HSVEB 452 46 -
ILPNQAVPIALIITPVRID EXON_HSV11 559 48 -
VPPACAVPVLLIVTPVSVC PRVULGNS1 430 37 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
EAATQNQADCQLWALLRR EXON_HSV11 219 219 -
EAATQNQADCQLWALLRR Q68978 219 219 -
EAATQNQADCQLWALLRR Q68979 93 93 -
EAQTRDQSESQLWALLRR O39290 168 168 -
EAATQNQADCQLWALLRR EXON_HSV2 209 209 -
EAATQNQADCQLWALLRR P89435 209 209 -
EAQTRDQSESQLWALLRR EXON_HSVEB 111 111 -
EAATRAQSESDLWTLLRR Q85039 91 91 -
EAATRGQAACDLWWLLRR Q65572 84 84 -
ERETRGQSENPLWHALRR EXON_HSV6Z 123 123 -
ESETRGQGDNAIWTLLRR EXON_VZVD 127 127 -
EALTRGQQDNELWDILRD O41954 121 121 -
EAITRGQYENNLWEIFRD Q66641 146 146 -
ERATSSQSLNPVWDALRD O40930 123 123 -
ERATSSQSLNPVWDALRD P88925 123 123 -
ERLTRGQGENVLWEVLRD O36386 117 117 -
ESMTRGQSENLMWDILRN EXON_EBV 107 107 -
ERETRGQSENPLWHALRK EXON_HSV6U 123 123 -
EEVTRGQYLNSLWDALRD EXON_HSVSA 119 119 -
EKESRGQSRNSVWHLLRM EXON_HCMVA 148 148 -
ERETRGQSTNPLWHAMRK EXON_HSV7J 114 114 -

Motif 2 width=24
Element Seqn Id St Int Rpt
CGVLMDGHTGMVGASLDILVCPRD EXON_HSV11 324 87 -
CGVLMDGHTGMVGASLDILVCPRD Q68978 324 87 -
CGVLMDGHTGMVGASLDILVCPRD Q68979 198 87 -
CGLLIDARTGVVGASLDMLVCDRD O39290 276 90 -
CGVLMDGHTGMVGASLDILVCPRD EXON_HSV2 314 87 -
CGVLMDGHTGMVGASLDILVCPRD P89435 314 87 -
CGLLMDARTGVVGASLDMLVCDRD EXON_HSVEB 219 90 -
CGLLLDPRTGMVGASLDLLVCDRD Q85039 207 98 -
CGLLIDPRTGALGASIDMLVCDRD Q65572 202 100 -
LGLLLDPSSGVFGASLDACFGISF EXON_HSV6Z 197 56 -
CGLLIDVRTGLIGASLDVLVCDRD EXON_VZVD 244 99 -
CGFLPSAIDGIFGVSLDTAFNVFT O41954 203 64 -
VGFLQSPKDGIFGVSLDMCANVSV Q66641 228 64 -
YGFMQSPQNGIFGVSLDFAANVKT O40930 205 64 -
YGFMQSPQNGIFGVSLDFAANVKT P88925 205 64 -
FGFMLSPNDGIFGVSLDMCTNASM O36386 200 65 -
FGFMISPTDGIFGVSLDLCVNVES EXON_EBV 187 62 -
LGLLLDPSSGVFGASLDACFGISF EXON_HSV6U 197 56 -
CGFMQSPLDGIFGVSLDYCTNVET EXON_HSVSA 202 65 -
LGLLIDPTSGLLGASMDLCFGVLK EXON_HCMVA 238 72 -
LGLLLDPSSGVFGASIDSYYGISF EXON_HSV7J 189 57 -

Motif 3 width=18
Element Seqn Id St Int Rpt
TPLAFYEVKCRAKYAFDP EXON_HSV11 358 10 -
TPLAFYEVKCRAKYAFDP Q68978 358 10 -
TPLAFYEVKCRAKYAFDP Q68979 232 10 -
TTLDFFEIKCRAKYLFDP O39290 311 11 -
TPLAFYEVKCRAKYAFDP EXON_HSV2 348 10 -
TPLAFYEVKCRAKYAFDP P89435 348 10 -
TTLDFFEIKCRAKYLFDP EXON_HSVEB 254 11 -
TEMRFFEIKCRAKYLFSA Q85039 242 11 -
TALGVFEIKCRAKYAFDP Q65572 237 11 -
EKALIFEIKFRYKYLRDK EXON_HSV6Z 230 9 -
TDISFFEIKCRAKYLFDP EXON_VZVD 280 12 -
PDSIVYEIKSRYKYQFSK O41954 236 9 -
ADAEIYEIKCRFKYNYSK Q66641 261 9 -
PNCKVYEIKCRFKYTFAK O40930 238 9 -
PNCKVYEIKCRFKYTFAK P88925 238 9 -
STTTIYEIKCRYKYLFSK O36386 233 9 -
DRSCIYEIKCRFKYLFSK EXON_EBV 219 8 -
EKALIFEIKFKYKYLRDK EXON_HSV6U 230 9 -
PDTEVYEIKSRFKYLFDK EXON_HSVSA 235 9 -
PCARVYEIKCRYKYLRKK EXON_HCMVA 272 10 -
DKVVIFELKFRYKYLREK EXON_HSV7J 222 9 -

Motif 4 width=17
Element Seqn Id St Int Rpt
RAFIRSIPKPSVRYFAP EXON_HSV11 399 23 -
RAFIRSIPKPSVRYFAP Q68978 399 23 -
RAFIRSIPKPSVRYFAP Q68979 273 23 -
RKFLRSIKNPAVEYFAS O39290 351 22 -
RAFIRSIPNPGVRYFAP EXON_HSV2 389 23 -
RAFIRSIPNPGVRYFAP P89435 389 23 -
RKFLRSIKNPAVEYFAP EXON_HSVEB 294 22 -
RGFLYSIARPGVEFFEG Q85039 282 22 -
RAFLRSVRRPGVEHCPP Q65572 276 21 -
SDFILSHPVPAIEFRER EXON_HSV6Z 265 17 -
RDFLYTIKNPCVSFFGP EXON_VZVD 321 23 -
IKFINCISRPAVEYVPH O41954 276 22 -
IRFLSGINRPAVEYVPP Q66641 301 22 -
KDFFYSISKPAVEYVGL O40930 278 22 -
KDFFYSISKPAVEYVGL P88925 278 22 -
IDFIQSIQKPTVEYVSR O36386 273 22 -
IRFINSIARPTVEYVPD EXON_EBV 259 22 -
SDFILSHPVPVIEFRER EXON_HSV6U 265 17 -
IKFIFSVSKPAIEFVPS EXON_HSVSA 275 22 -
ASLLQSHPVPGVEFRGE EXON_HCMVA 307 17 -
AKFILSHPIPAIEYREN EXON_HSV7J 257 17 -

Motif 5 width=21
Element Seqn Id St Int Rpt
PVFANPRHPNFKQILVQGYVL EXON_HSV11 490 74 -
PVFANPRHPNFKQILVQGYVL Q68978 490 74 -
PVFANPRHPNFKQILVQGYVL Q68979 364 74 -
PVFANPRHPNFKQILVQAYVL O39290 442 74 -
PIFANPRHPNFKQILVQGYVL EXON_HSV2 480 74 -
PIFANPRHPNFKQILVQGYVL P89435 480 74 -
PVFANPRHPNFKQILVQAYVL EXON_HSVEB 385 74 -
PVFANPRHANFRQILVQSYVV Q85039 372 73 -
PLFANPRHANFKQILVQSYVL Q65572 369 76 -
NVFVNPKHRYFFQSLLQQYVM EXON_HSV6Z 356 74 -
PVFANPRHANFKQIAVQTYVL EXON_VZVD 411 73 -
DVFVNPAHNYFYQVALQNMVV O41954 362 69 -
DVFINPEHSYFYQILLQYKVV Q66641 387 69 -
NLFVNVRHSYFYQVLLQSSIV O40930 364 69 -
NLFVNVRHSYFYQVLLQSSIV P88925 364 69 -
DIYINPRHAYYHQCMLQYKIV O36386 360 70 -
NIFINPRHNYFYQVLLQYKIV EXON_EBV 345 69 -
NVFVNPKHRYFFQSLLQQYVM EXON_HSV6U 356 74 -
GIFMNPRHTYFYQVALQHRVV EXON_HSVSA 361 69 -
PAFVNPRHQYYFQMLIQQYVL EXON_HCMVA 452 128 -
DAFINPRHRYYFQSILQQYVM EXON_HSV7J 348 74 -

Motif 6 width=19
Element Seqn Id St Int Rpt
ILPNQAVPIALIITPVRID EXON_HSV11 559 48 -
ILPNQAVPIALIITPVRID Q68978 559 48 -
ILPNQAVPIALIITPVRID Q68979 433 48 -
VHSSCAIPILLIVTPVIVD O39290 509 46 -
ILPDQAVPIALIITPVRVE EXON_HSV2 553 52 -
ILPDQAVPIALIITPVRVE P89435 553 52 -
VHSSCAIPILLFVTPVIVD EXON_HSVEB 452 46 -
VPPACAVPVLLIVTPVSVC Q85039 430 37 -
VPREGAVPVLLIATPVVVD Q65572 431 41 -
EYIEEEIPLALIVTPVAPN EXON_HSV6Z 423 46 -
IPPHCAVPVIAVLTPIEVD EXON_VZVD 478 46 -
LDTTDEIPVALIITPVRIP O41954 427 44 -
LDSACEIPVLLILTPVYIP Q66641 450 42 -
LDPHVEIPTLLIVTPVYFP O40930 427 42 -
LDPHVEIPTLLIVTPVYFP P88925 427 42 -
VDASVEIPVLLIITPVFVP O36386 426 45 -
LDASVEIPVAVLVTPVVLP EXON_EBV 411 45 -
EYIEEEIPLALIVTPVAPN EXON_HSV6U 423 46 -
LEKTVEIPVFIIITPVYIP EXON_HSVSA 424 42 -
VFHCDHIPLLLIVTPVVFD EXON_HCMVA 519 46 -
EYLEEEIPLILLITPITID EXON_HSV7J 415 46 -