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PR00898

Identifier
VASOPRSNV2R  [View Relations]  [View Alignment]  
Accession
PR00898
No. of Motifs
8
Creation Date
15-APR-1998  (UPDATE 07-JUN-1999)
Title
Vasopressin V2 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00896 VASOPRESSINR
INTERPRO; IPR000161
GCRDB; GCR_0364; GCR_0798; GCR_0383; GCR_0773; GCR_0654
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Vasopressin and oxytocin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.284-291.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Vasopressin and oxytocin are members of the neurohypophyseal hormone family
found in all mammalian species [6]. They are present in high levels in the
posterior pituitary. Vasopressin has an essential role in the control of 
the water content of the body, acting in the kidney to increase water and
sodium absorption [6]. In higher concentrations, vasopressin stimulates
contraction of vascular smooth muscle, stimulates glycogen breakdown in the
liver, induces platelet activation, and evokes release of corticotrophin 
from the anterior pituitary [6]. Vasopressin and its analogues are used
clinically to treat diabetes insipidus [6]. 
 
The V2 receptor is found in high levels in the osmoregulatory epithelia of
the terminal urinary tract, where it stimulates water reabsorption [6]. It
is also present in lower levels in the endothelium and blood vessels of some
species, where it induces vasodilation [6]. In the CNS, binding sites are
found in the subiculum, with lower levels in caudate-putamen and islands 
of Calleja [6]. The receptor is involved in an effector pathway that forms
cAMP through activation of Gs [6].
 
VASOPRSNV2R is an 8-element fingerprint that provides a signature for
vasopressin V2 receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from short conserved
sections spanning the full alignment length, focusing on those regions
that characterise the vasopressin V2 receptors but distinguish them from
the rest of the vasopressin family - motifs 1 and 2 reside at the N-terminus; 
motif 3 spans the first cytoplasmic loop; motif 4 spans the second 
cytoplasmic loop; motifs 5 and 6 span the third cytoplasmic loop; and 
motifs 7 and 8 reside at the C-terminus. A single iteration on OWL30.1 was
required to reach convergence, no further sequences being identified
beyond the starting set.
 
An update on SPTR37_9f identified a true set of 6 sequences, and 1
partial match.
Summary Information
   6 codes involving  8 elements
0 codes involving 7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
866666666
700000000
611111100
500000000
400000000
300000000
200000000
12345678
True Positives
O77808        O88721        V2R_BOVIN     V2R_HUMAN     
V2R_PIG V2R_RAT
True Positive Partials
Codes involving 6 elements
O43192
Sequence Titles
O77808      VASOPRESSIN V2 RECEPTOR - CANIS FAMILIARIS (DOG). 
O88721 VASOPRESSIN RECEPTOR TYPE II - MUS MUSCULUS (MOUSE).
V2R_BOVIN Vasopressin V2 receptor (Renal-type arginine vasopressin receptor) (Antidiuretic hormone receptor) (AVPR V2) - Bos taurus (Bovine).
V2R_HUMAN Vasopressin V2 receptor (Renal-type arginine vasopressin receptor) (Antidiuretic hormone receptor) (AVPR V2) - Homo sapiens (Human).
V2R_PIG Vasopressin V2 receptor (Renal-type arginine vasopressin receptor) (Antidiuretic hormone receptor) (AVPR V2) - Sus scrofa (Pig).
V2R_RAT Vasopressin V2 receptor (Renal-type arginine vasopressin receptor) (Antidiuretic hormone receptor) (AVPR V2) - Rattus norvegicus (Rat).

O43192 VASOPRESSIN RECEPTOR TYPE 2 - HOMO SAPIENS (HUMAN).
Scan History
OWL30_1    1  50   NSINGLE    
SPTR37_9f 2 7 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
TVSAVPGLFSPPSSPSN V2R_RAT 6 6 -
TTSAVPRALSWPAAPGN V2R_PIG 5 5 -
TTSAVPGHPSLPSLPSN V2R_HUMAN 6 6 -
TTSAVPWHLSQPTPAGN V2R_BOVIN 6 6 -

Motif 2 width=15
Element Seqn Id St Int Rpt
NSSQEELLDDRDPLL V2R_RAT 22 -1 -
NGSEREPLDDRDPLL V2R_PIG 21 -1 -
NSSQERPLDTRDPLL V2R_HUMAN 22 -1 -
NGSEGELLTARDPLL V2R_BOVIN 22 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ALIRRGRRGRWAPMHV V2R_RAT 61 24 -
ALVRRGRRGRWAPMHV V2R_PIG 60 24 -
ALARRGRRGHWAPIHV V2R_HUMAN 61 24 -
ALVRRGRRGRWAPMHV V2R_BOVIN 61 24 -

Motif 4 width=16
Element Seqn Id St Int Rpt
MLAYRHGGGARWNRPV V2R_RAT 145 68 -
MLAYRHGGGARWNRPV V2R_PIG 144 68 -
MLAYRHGSGAHWNRPV V2R_HUMAN 145 68 -
MLAHRHGGGTHWNRPV V2R_BOVIN 145 68 -

Motif 5 width=16
Element Seqn Id St Int Rpt
QVLIFREIHASLVPGP V2R_RAT 225 64 -
QVLIFREIHTSLVPGP V2R_PIG 224 64 -
QVLIFREIHASLVPGP V2R_HUMAN 225 64 -
QVLIFREIHASLGPGP V2R_BOVIN 224 63 -

Motif 6 width=20
Element Seqn Id St Int Rpt
RAGRRRRGRRTGSPSEGAHV V2R_RAT 243 2 -
RAGGHRGGRRAGSPREGARV V2R_PIG 242 2 -
RPGGRRRGRRTGSPGEGAHV V2R_HUMAN 243 2 -
RAGGPRRGCRPGSPAEGARV V2R_BOVIN 242 2 -

Motif 7 width=15
Element Seqn Id St Int Rpt
ASFSSSVSSELRSLL V2R_RAT 326 63 -
ASFSSSISSELRSLL V2R_PIG 325 63 -
ASFSSSVSSELRSLL V2R_HUMAN 326 63 -
ASFSSSISSELRSLL V2R_BOVIN 325 63 -

Motif 8 width=20
Element Seqn Id St Int Rpt
SLGPQDESCATASSSLMKDT V2R_RAT 350 9 -
SLRPQEESCATASSFSARDT V2R_PIG 349 9 -
SLGPQDESCTTASSSLAKDT V2R_HUMAN 350 9 -
SPGPQEESCATASSFLAKDT V2R_BOVIN 349 9 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
TTSAVPGALSSPSSPSN O88721 6 6 -
TVSAVPGLFSPPSSPSN V2R_RAT 6 6 -
TTSAVPRALSWPAAPGN V2R_PIG 5 5 -
TTSAVPGHPSLPSLPSN V2R_HUMAN 6 6 -
TTSAVPRTLSPPTPAGN O77808 6 6 -
TTSAVPWHLSQPTPAGN V2R_BOVIN 6 6 -

Motif 2 width=15
Element Seqn Id St Int Rpt
NSSQEELLDDRDPLL O88721 22 -1 -
NSSQEELLDDRDPLL V2R_RAT 22 -1 -
NGSEREPLDDRDPLL V2R_PIG 21 -1 -
NSSQERPLDTRDPLL V2R_HUMAN 22 -1 -
GNGSRELLDTRDPLL O77808 21 -2 -
NGSEGELLTARDPLL V2R_BOVIN 22 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ALIRRGRRGRWAPMHV O88721 61 24 -
ALIRRGRRGRWAPMHV V2R_RAT 61 24 -
ALVRRGRRGRWAPMHV V2R_PIG 60 24 -
ALARRGRRGHWAPIHV V2R_HUMAN 61 24 -
ALARRVRRGRWAPMHV O77808 60 24 -
ALVRRGRRGRWAPMHV V2R_BOVIN 61 24 -

Motif 4 width=16
Element Seqn Id St Int Rpt
MLAYRHGGGARWNRPV O88721 145 68 -
MLAYRHGGGARWNRPV V2R_RAT 145 68 -
MLAYRHGGGARWNRPV V2R_PIG 144 68 -
MLAYRHGSGAHWNRPV V2R_HUMAN 145 68 -
MLAYRHGGGARWNRPV O77808 144 68 -
MLAHRHGGGTHWNRPV V2R_BOVIN 145 68 -

Motif 5 width=16
Element Seqn Id St Int Rpt
QVLIFREIHASLVPGP O88721 225 64 -
QVLIFREIHASLVPGP V2R_RAT 225 64 -
QVLIFREIHTSLVPGP V2R_PIG 224 64 -
QVLIFREIHASLVPGP V2R_HUMAN 225 64 -
QVLIFREIHSSLVPGP O77808 224 64 -
QVLIFREIHASLGPGP V2R_BOVIN 224 63 -

Motif 6 width=20
Element Seqn Id St Int Rpt
RAGRRRRGHRTGSPSEGAHV O88721 243 2 -
RAGRRRRGRRTGSPSEGAHV V2R_RAT 243 2 -
RAGGHRGGRRAGSPREGARV V2R_PIG 242 2 -
RPGGRRRGRRTGSPGEGAHV V2R_HUMAN 243 2 -
RAGGCRGGHRTGSPSEGARV O77808 242 2 -
RAGGPRRGCRPGSPAEGARV V2R_BOVIN 242 2 -

Motif 7 width=15
Element Seqn Id St Int Rpt
ASFSSSVSSELRSLL O88721 326 63 -
ASFSSSVSSELRSLL V2R_RAT 326 63 -
ASFSSSISSELRSLL V2R_PIG 325 63 -
ASFSSSVSSELRSLL V2R_HUMAN 326 63 -
AFFSSSVSSELRSLF O77808 325 63 -
ASFSSSISSELRSLL V2R_BOVIN 325 63 -

Motif 8 width=20
Element Seqn Id St Int Rpt
SLGPQDESCATASSSLMKDT O88721 350 9 -
SLGPQDESCATASSSLMKDT V2R_RAT 350 9 -
SLRPQEESCATASSFSARDT V2R_PIG 349 9 -
SLGPQDESCTTASSSLAKDT V2R_HUMAN 350 9 -
SLGPQEESCATASSFLAKDT O77808 349 9 -
SPGPQEESCATASSFLAKDT V2R_BOVIN 349 9 -