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PR00890

Identifier
TRANSGELIN  [View Relations]  [View Alignment]  
Accession
PR00890
No. of Motifs
6
Creation Date
07-JUN-1998  (UPDATE 22-JUN-1999)
Title
Smooth muscle protein 22-alpha (transgelin) signature
Database References
PRINTS; PR00888 SM22CALPONIN
INTERPRO; IPR001061
Literature References
1. SOLWAY, J., SELTZER, J., SAMAHA, F.F., KIM, S., ALGER, L.E., NIU, Q.,
MORRISEY, E.E., IP, H.S. AND PARMACEK, M.S.
Structure and expression of a smooth muscle cell-specific gene, SM22 alpha. 
J.BIOL.CHEM. 270 13460-13469 (1995). 
 
2. PRINJHA, R.K., SHAPLAND, C.E., HSUAN, J.J., TOTTY, N.F., MASON, I.J.
AND LAWSON, D.
Cloning and sequencing of cDNAs encoding the actin cross-linking protein
transgelin defines a new family of actin-associated proteins. 
CELL MOTIL.CYTOSKEL. 28 243-255 (1994). 

Documentation
SM22-alpha (transgelin) is expressed exclusively in smooth muscle-containing
tissues of adult animals and is one of the earliest markers of differentiated
smooth muscle cells (SMCs) [1]. SM22-alpha mRNA is expressed at high levels
in the aorta, uterus, lung, and intestine, and in primary cultures of rat 
aortic SMCs, and the SMC line, A7r5 [1].
 
Transgelin is the product of a single gene that is conserved in yeast,
Drosophila, molluscs and humans [2], and is highly similar to several other
proteins of unknown function (including mouse p27 and human WS3-10). The 
smaller domains of similarity shared between transgelin and other proteins, 
such as rat NP25, chicken calponins alpha and beta, and Drosophila mp20,
suggest that these proteins may be classified as members of a new transgelin
multigene family [2]. 
 
TRANSGELIN is a 6-element fingerprint that provides a signature for smooth
muscle 22-alpha proteins. The fingerprint was derived from an initial 
alignment of 5 sequences: the motifs were drawn from short conserved 
sections spanning virtually the full alignment length, focusing on those
regions that characterise the SM22 proteins but distinguish them from 
the rest of the calponin family - motif 5 includes a putative (RGD) cell
attachment site. Two iterations on OWL30.2 were required to reach
convergence, at which point a true set comprising 10 sequences was
identified. Four partial matches were also found, all of which are
SM22/calponin family members that fail to match one or more motifs.
 
An update on SPTR37_9f identified a true set of 6 sequences, and 4
partial matches.
Summary Information
   6 codes involving  6 elements
0 codes involving 5 elements
1 codes involving 4 elements
1 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
6666666
5000000
4011101
3011001
2010001
123456
True Positives
NP25_RAT      SM22_CHICK    SM22_HUMAN    SM22_MOUSE    
SM22_RAT SM2H_HUMAN
True Positive Partials
Codes involving 4 elements
Q24799
Codes involving 3 elements
Q93713
Codes involving 2 elements
MP20_DROME
Sequence Titles
NP25_RAT    NEURONAL PROTEIN NP25 - RATTUS NORVEGICUS (RAT). 
SM22_CHICK SMOOTH MUSCLE PROTEIN 22-ALPHA (SM22-ALPHA) - GALLUS GALLUS (CHICKEN).
SM22_HUMAN SMOOTH MUSCLE PROTEIN 22-ALPHA (SM22-ALPHA) (TRANSGELIN) (WS3-10) (22 KD ACTIN-BINDING PROTEIN) - HOMO SAPIENS (HUMAN).
SM22_MOUSE SMOOTH MUSCLE PROTEIN 22-ALPHA (SM22-ALPHA) (TRANSGELIN) (ACTIN- ASSOCIATED PROTEIN P27) - MUS MUSCULUS (MOUSE).
SM22_RAT SMOOTH MUSCLE PROTEIN 22-ALPHA (SM22-ALPHA) (TRANSGELIN) - RATTUS NORVEGICUS (RAT).
SM2H_HUMAN SM22-ALPHA HOMOLOG (HA1756) (KIAA0120) - HOMO SAPIENS (HUMAN).

Q24799 MYOPHILIN ANTIGEN - ECHINOCOCCUS GRANULOSUS.

Q93713 F43G9.9 PROTEIN - CAENORHABDITIS ELEGANS.

MP20_DROME MUSCLE-SPECIFIC PROTEIN 20 - DROSOPHILA MELANOGASTER (FRUIT FLY).
Scan History
OWL30_2    2  100  NSINGLE    
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
IVMQCGPDVGRPDRGRLGFQ SM22_RAT 33 33 -
IIVQCGPDVGRPDRGPLGFQ SM22_HUMAN 33 33 -
IVVQCGPDVGRPDRGRLGFQ SM22_MOUSE 33 33 -
IVAQCGSSVGRPDRGRLGFQ SM22_CHICK 33 33 -
ITTQCRKDVGRPQPGRENFQ SM2H_HUMAN 34 34 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LSKLVNSLYPEGSKPV SM22_RAT 61 8 -
LSKLVNSLYPEGSKPV SM22_HUMAN 61 8 -
LSKLVNSLYPEGSKPV SM22_MOUSE 61 8 -
LSQLVNSLYPDGSKPV SM22_CHICK 61 8 -
LCELINALYPEGQAPV SM2H_HUMAN 62 8 -

Motif 3 width=14
Element Seqn Id St Int Rpt
SMVFKQMEQVAQFL SM22_RAT 84 7 -
SMVFKQMEQVAQFL SM22_HUMAN 84 7 -
SMVFKQMEQVAQFL SM22_MOUSE 84 7 -
TMVFKQMEQIAQFL SM22_CHICK 84 7 -
TMAFKQMEQISQFL SM2H_HUMAN 84 6 -

Motif 4 width=10
Element Seqn Id St Int Rpt
EGKDMAAVQR SM22_RAT 118 20 -
EGKDMAAVQR SM22_HUMAN 118 20 -
EGKDMAAVQR SM22_MOUSE 118 20 -
EAKDMAAVQR SM22_CHICK 118 20 -
EGKNMACVQR SM2H_HUMAN 118 20 -

Motif 5 width=20
Element Seqn Id St Int Rpt
LAVTKNDGHYRGDPNWFMKK SM22_RAT 135 7 -
LAVTKNDGHYRGDPNWFMKK SM22_HUMAN 135 7 -
LAVTKNDGNYRGDPNWFMKK SM22_MOUSE 135 7 -
LAVTKNDGHYHGDPNWFMKK SM22_CHICK 135 7 -
LAVARDDGLFSGDPNWFPKK SM2H_HUMAN 135 7 -

Motif 6 width=13
Element Seqn Id St Int Rpt
EFTDSQLQEGKHV SM22_RAT 161 6 -
EFTESQLQEGKHV SM22_HUMAN 161 6 -
DFTDSQLQEGKHV SM22_MOUSE 161 6 -
EFSESQLKEGKNI SM22_CHICK 161 6 -
NFSDNQLQEGKNV SM2H_HUMAN 161 6 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
IVMQCGPDVGRPDRGRLGFQ SM22_RAT 33 33 -
IIVQCGPDVGRPDRGPLGFQ SM22_HUMAN 33 33 -
IVVQCGPDVGRPDRGRLGFQ SM22_MOUSE 33 33 -
IVAQCGSSVGRPDRGRLGFQ SM22_CHICK 33 33 -
ITTQCRKDVGRPQPGRENFQ SM2H_HUMAN 34 34 -
IILQCAEDIEHPPPGRTHFQ NP25_RAT 34 34 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LSKLVNSLYPEGSKPV SM22_RAT 61 8 -
LSKLVNSLYPEGSKPV SM22_HUMAN 61 8 -
LSKLVNSLYPEGSKPV SM22_MOUSE 61 8 -
LSQLVNSLYPDGSKPV SM22_CHICK 61 8 -
LCELINALYPEGQAPV SM2H_HUMAN 62 8 -
LCKLINSLYPPGQEPI NP25_RAT 62 8 -

Motif 3 width=14
Element Seqn Id St Int Rpt
SMVFKQMEQVAQFL SM22_RAT 84 7 -
SMVFKQMEQVAQFL SM22_HUMAN 84 7 -
SMVFKQMEQVAQFL SM22_MOUSE 84 7 -
TMVFKQMEQIAQFL SM22_CHICK 84 7 -
TMAFKQMEQISQFL SM2H_HUMAN 84 6 -
KMAFKQMEQISQFL NP25_RAT 84 6 -

Motif 4 width=10
Element Seqn Id St Int Rpt
EGKDMAAVQR SM22_RAT 118 20 -
EGKDMAAVQR SM22_HUMAN 118 20 -
EGKDMAAVQR SM22_MOUSE 118 20 -
EAKDMAAVQR SM22_CHICK 118 20 -
EGKNMACVQR SM2H_HUMAN 118 20 -
EGKDMAAVQR NP25_RAT 118 20 -

Motif 5 width=20
Element Seqn Id St Int Rpt
LAVTKNDGHYRGDPNWFMKK SM22_RAT 135 7 -
LAVTKNDGHYRGDPNWFMKK SM22_HUMAN 135 7 -
LAVTKNDGNYRGDPNWFMKK SM22_MOUSE 135 7 -
LAVTKNDGHYHGDPNWFMKK SM22_CHICK 135 7 -
LAVARDDGLFSGDPNWFPKK SM2H_HUMAN 135 7 -
VAVTKDDGCYRGEPSWFHRK NP25_RAT 135 7 -

Motif 6 width=13
Element Seqn Id St Int Rpt
EFTDSQLQEGKHV SM22_RAT 161 6 -
EFTESQLQEGKHV SM22_HUMAN 161 6 -
DFTDSQLQEGKHV SM22_MOUSE 161 6 -
EFSESQLKEGKNI SM22_CHICK 161 6 -
NFSDNQLQEGKNV SM2H_HUMAN 161 6 -
GFSEEQLRQGQNV NP25_RAT 161 6 -