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PR00880

Identifier
ACHEINSECT  [View Relations]  [View Alignment]  
Accession
PR00880
No. of Motifs
9
Creation Date
02-JUL-1998  (UPDATE 28-JUL-1999)
Title
Insect acetylcholinesterase signature
Database References
PRINTS; PR00878 CHOLNESTRASE
INTERPRO; IPR001445
Literature References
1. VOET, D. AND VOET, J.G.
Biochemical communications: Hormones and neurotransmission.
BIOCHEMISTRY 34-4 1298-1299 (1995).
 
2. COUSIN, X., HOTELIER, T., GILES, K., TOUTANT, J.P. AND CHATONNET, A.
aCHEdb: the database system for ESTHER, the a/b fold family of proteins and
the Cholinesterase gene server.
NUCLEIC ACIDS RES. 26 226-228 (1998).
 
3. THE ESTHER DATABASE
http://meleze.ensam.inra.fr/cholinesterase/definitions.html
 
4. LIU, H.T., STILWELL, G., ANTHONY, N., ROCHELEAU, T. AND
FFRENCH-CONSTANT, R.H.
Analysis of a mosquito acetylcholinesterase gene promotor.
INSECT MOL.BIOL. 7 11-17 (1998).
 
5. GUEDES, R.N.C., ZHU, K.Y., KAMBHAMPATI, S. AND DOUER, B.A.
Characterisation of acetylcholinesterase purified from the lesser grain
borer, Rhyzopertha dominica.
COMP.BIOCHEM.PHYSIOL. 119 205-210 (1998).
 
6. CHOLINESTERASES
http://bnlstb.bio.bnl.gov:8000/disk$3/giles/che.html

Documentation
Acetylcholine is involved in the transfer of messages across a variety of
synapses in vertebrates and invertebrates. The diverse physiological effects
attributable to this molecule arise from the presence of specific receptors
in the postsynaptic cell membranes. Several classes of receptor are known, 
most of which translate the binding of acetylcholine into the opening of 
channels for the passage of ions, such as sodium and potassium.
 
Acetylcholinesterase is an enzyme that catalyses the hydrolysis of 
acetylcholine to choline and acetate:
 
     Acetylcholine + H(2)O -> Choline + Acetate 	     
 
The enzyme also acts on a variety of acetic esters and catalyses trans-
acetylations. It is found in, or attached to, cellular or basement membranes
of presynaptic cholinergic neurons and postsynaptic cholinoceptive cells.
To prevent continuous firing of nerve impulses, acetylcholinesterase has a
high K(cat) (~14000/s), to ensure that acetylcholine is broken down quickly.
 
Cholinesterases constitute a family of enzymes that fall into two main
types, depending on their substrate specificity: enzymes that preferentially
hydrolyse acetyl esters are termed acetylcholinesterase (AChE) (EC 3.1.1.7); 
those that prefer other types of ester, such as butyrylcholine are termed 
butyrylcholinesterase (BChE) (EC 3.1.1.8). 
 
Due to the importance of this enzyme, it is often targeted by insecticides,
and is especially relevant in efforts to eradicate mosquitos from certain
areas of the world [4,5].
 
ACHEINSECT is a 9-element fingerprint that provides a signature for insect
acetylcholinesterases. The fingerprint was derived from an initial alignment
of 4 sequences: the motifs were drawn from short conserved sections spanning
the N-terminal portion of the alignment. Two iterations on OWL30.2 were 
required to reach convergence, at which point a true set comprising 5
sequences was identified. Several partial matches were also found, all of
which are Drosophila AChE fragments.
 
An update on SPTR37_9f identified a true set of 4 sequences, and 1
partial match.
Summary Information
4 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9444444444
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
ACES_ANOST    ACES_DROME    ACES_LEPDE    P91954        
Sequence Titles
ACES_ANOST  ACETYLCHOLINESTERASE PRECURSOR (EC 3.1.1.7) - ANOPHELES STEPHENSI (INDO-PAKISTAN MALARIA MOSQUITO). 
ACES_DROME ACETYLCHOLINESTERASE PRECURSOR (EC 3.1.1.7) (ACHE) - DROSOPHILA MELANOGASTER (FRUIT FLY).
ACES_LEPDE ACETYLCHOLINESTERASE PRECURSOR (EC 3.1.1.7) - LEPTINOTARSA DECEMLINEATA (COLORADO POTATO BEETLE).
P91954 ACETYLCHOLINESTERASE - LUCILIA CUPRINA (GREENBOTTLE FLY) (AUSTRALIAN SHEEP BLOWFLY).
Scan History
OWL30_2    2  120  NSINGLE    
SPTR37_9f 2 75 NSINGLE
Initial Motifs
Motif 1  width=6
Element Seqn Id St Int Rpt
QTSSGP S66236 36 36 -
QTSSGP ACES_ANOST 37 37 -
QTSSGP ACES_DROME 46 46 -
ETTSGL ACES_LEPDE 45 45 -

Motif 2 width=9
Element Seqn Id St Int Rpt
QGREVHVYT ACES_DROME 60 8 -
QGREVHVFN ACES_ANOST 51 8 -
LGREVHVFN S66236 50 8 -
LGREVHVFT ACES_LEPDE 59 8 -

Motif 3 width=5
Element Seqn Id St Int Rpt
EPWHG S66236 79 20 -
EPWHG ACES_ANOST 80 20 -
DPWHG ACES_LEPDE 88 20 -
EPWHG ACES_DROME 89 20 -

Motif 4 width=6
Element Seqn Id St Int Rpt
ERYEYF ACES_DROME 107 13 -
ERYEYF S66236 97 13 -
ERYEYF ACES_ANOST 98 13 -
ERYEYF ACES_LEPDE 106 13 -

Motif 5 width=6
Element Seqn Id St Int Rpt
KTRLRH S66236 131 28 -
KARLRH ACES_DROME 141 28 -
KTRLRH ACES_ANOST 132 28 -
RLRIRH ACES_LEPDE 140 28 -

Motif 6 width=10
Element Seqn Id St Int Rpt
NAEILAAVGN ACES_ANOST 187 49 -
NAEMLAAVGN S66236 186 49 -
DADIIAATSD ACES_LEPDE 179 33 -
NADIMAAVGN ACES_DROME 201 54 -

Motif 7 width=5
Element Seqn Id St Int Rpt
RWLKE S66236 236 40 -
RWLKD ACES_DROME 254 43 -
RWLKE ACES_ANOST 239 42 -
RWIKD ACES_LEPDE 231 42 -

Motif 8 width=7
Element Seqn Id St Int Rpt
KTISLQQ ACES_LEPDE 329 93 -
KTISVQQ S66236 334 93 -
KTISVQQ ACES_DROME 352 93 -
KTISVQQ ACES_ANOST 337 93 -

Motif 9 width=8
Element Seqn Id St Int Rpt
GEEWPNFS ACES_DROME 564 205 -
DVNWPLYT ACES_LEPDE 542 206 -
GEHWPLYT ACES_ANOST 550 206 -
GEHWPVYT S66236 547 206 -
Final Motifs
Motif 1  width=6
Element Seqn Id St Int Rpt
QTSSGP ACES_DROME 46 46 -
QTTSGP P91954 105 105 -
QTSSGP ACES_ANOST 37 37 -
ETTSGL ACES_LEPDE 45 45 -

Motif 2 width=9
Element Seqn Id St Int Rpt
QGREVHVYT ACES_DROME 60 8 -
QGREVHVFT P91954 119 8 -
QGREVHVFN ACES_ANOST 51 8 -
LGREVHVFT ACES_LEPDE 59 8 -

Motif 3 width=5
Element Seqn Id St Int Rpt
EPWHG ACES_DROME 89 20 -
EPWHG P91954 148 20 -
EPWHG ACES_ANOST 80 20 -
DPWHG ACES_LEPDE 88 20 -

Motif 4 width=6
Element Seqn Id St Int Rpt
ERYEYF ACES_DROME 107 13 -
ERYEYF P91954 166 13 -
ERYEYF ACES_ANOST 98 13 -
ERYEYF ACES_LEPDE 106 13 -

Motif 5 width=6
Element Seqn Id St Int Rpt
KARLRH ACES_DROME 141 28 -
KARLRH P91954 200 28 -
KTRLRH ACES_ANOST 132 28 -
RLRIRH ACES_LEPDE 140 28 -

Motif 6 width=10
Element Seqn Id St Int Rpt
NADIMAAVGN ACES_DROME 201 54 -
NADIMSAVGN P91954 258 52 -
NAEILAAVGN ACES_ANOST 187 49 -
DADIIAATSD ACES_LEPDE 179 33 -

Motif 7 width=5
Element Seqn Id St Int Rpt
RWLKD ACES_DROME 254 43 -
RWLKE P91954 310 42 -
RWLKE ACES_ANOST 239 42 -
RWIKD ACES_LEPDE 231 42 -

Motif 8 width=7
Element Seqn Id St Int Rpt
KTISVQQ ACES_DROME 352 93 -
KTISVQQ P91954 408 93 -
KTISVQQ ACES_ANOST 337 93 -
KTISLQQ ACES_LEPDE 329 93 -

Motif 9 width=8
Element Seqn Id St Int Rpt
GEEWPNFS ACES_DROME 564 205 -
GEEWPNFS P91954 620 205 -
GEHWPLYT ACES_ANOST 550 206 -
DVNWPLYT ACES_LEPDE 542 206 -