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PR00855

Identifier
PRSTNOIDFPR  [View Relations]  [View Alignment]  
Accession
PR00855
No. of Motifs
9
Creation Date
12-FEB-1998  (UPDATE 07-JUN-1999)
Title
Prostaglandin F receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01788 PROSTANOIDR; PR00428 PROSTAGLNDNR
INTERPRO; IPR000141
GCRDB; GCR_0963
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL,
Prostanoids.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp239-251.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Prostanoids (prostaglandins (PG) and thromboxanes (TX)) mediate a wide
variety of actions and play important physiological roles in the cardio-
vascular and immune systems, and in pain sensation in peripheral systems
[6]. PGI2 and TXA2 have opposing actions, involving regulation of the
interaction of platelets with the vascular endothelium, while PGE2, PGI2
and PGD2 are powerful vasodilators and potentiate the action of various
autocoids to induce plasma extravasation and pain sensation. To date,
evidence for at least 5 classes of prostanoid receptor has been obtained.
However, identification of subtypes and their distribution is hampered by
expression of more than one receptor within a tissue, coupled with poor
selectivity of available agonists and antagonists. 
 
FP receptors mediate contraction in a wide range of smooth muscle, including
intraocular, myomretrial and bronchial tissues, and are potent stimulants of
luteolysis [6]. The receptors activate the phosphoinositide pathway through
a pertussis-toxin-insensitive G protein, probably of the Gq/G11 class [6].
 
PRSTNOIDFPR is a 9-element fingerprint that provides a signature for the
prostanoid FP receptors. The fingerprint was derived from an initial 
alignment of 4 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas of 
the alignment that characterise the prostanoid FP receptors but distinguish
them from the rest of the prostanoid receptor family - motifs 1 and 2 span
the N-terminus; motif 3 spans the first cytoplasmic loop, leading into TM
domain 2; motifs 4 and 5 span the second external loop, leading into TM
domain 5; motif 6 spans the third cytoplasmic loop; motif 7 spans the third
external loop; and motifs 8 and 9 reside at the C-terminus. Two iterations
on OWL29.6 were required to reach convergence, at which point a true set
comprising 6 sequences was identified. Two partial matches were also found,
both of which are prostanoid FP receptor fragments.
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9555555555
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
PF2R_BOVIN    PF2R_HUMAN    PF2R_MOUSE    PF2R_RAT      
PF2R_SHEEP
Sequence Titles
PF2R_BOVIN  Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Bos taurus (Bovine). 
PF2R_HUMAN Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Homo sapiens (Human).
PF2R_MOUSE Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Mus musculus (Mouse).
PF2R_RAT Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Rattus norvegicus (Rat).
PF2R_SHEEP Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Ovis aries (Sheep).
Scan History
OWL30_0    2  50   NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
SMNSSKQPVSPAAGLIANTTCQ PF2R_MOUSE 2 2 -
SINSSKQPASSAAGLIANTTCQ PF2R_RAT 2 2 -
SMNNSKQLVSPAAALLSNTTCQ PF2R_HUMAN 2 2 -
STNSSIQPVSPESELLSNTTCQ PF2R_BOVIN 2 2 -

Motif 2 width=15
Element Seqn Id St Int Rpt
ENRLSVFFSIIFMTV PF2R_MOUSE 25 1 -
ENRLSVFFSIIFMTV PF2R_RAT 25 1 -
ENRLSVFFSVIFMTV PF2R_HUMAN 25 1 -
EEDLSISFSIIFMTV PF2R_BOVIN 25 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
KAYQRFRQKSKASFLL PF2R_MOUSE 53 13 -
KAYQRFRRKSKASFLL PF2R_RAT 53 13 -
KAYQRFRQKSKASFLL PF2R_HUMAN 53 13 -
KAYQRFRQKYKSSFLL PF2R_BOVIN 53 13 -

Motif 4 width=14
Element Seqn Id St Int Rpt
ILGHRDYQIQASRT PF2R_MOUSE 171 102 -
ILGHRDYQIQASRT PF2R_RAT 171 102 -
ILGHRDYKIQASRT PF2R_HUMAN 171 102 -
ILGHRDYKIQASRT PF2R_BOVIN 171 102 -

Motif 5 width=18
Element Seqn Id St Int Rpt
YNTEHIEDWEDRFYLLFF PF2R_MOUSE 188 3 -
YNTEHIEDWEDRFYLLFF PF2R_RAT 188 3 -
YNTEDIKDWEDRFYLLLF PF2R_HUMAN 188 3 -
YKTDEIKDWEDRFYLLLF PF2R_BOVIN 188 3 -

Motif 6 width=17
Element Seqn Id St Int Rpt
LRVKFRSQQHRQGRSHH PF2R_MOUSE 228 22 -
LRVKFRSQQHRQGRSHH PF2R_RAT 228 22 -
LRVKFKSQQHRQGRSHH PF2R_HUMAN 228 22 -
LKVKFRSQQHRQGRSHH PF2R_BOVIN 228 22 -

Motif 7 width=17
Element Seqn Id St Int Rpt
IAINGNNSPVTCETTLF PF2R_MOUSE 272 27 -
IAINGNNSPVTCETTLF PF2R_RAT 272 27 -
IGINGNHSLETCETTLF PF2R_HUMAN 272 27 -
IGMNIQDFKDSCERTLF PF2R_BOVIN 272 27 -

Motif 8 width=18
Element Seqn Id St Int Rpt
NLYKLASRCCGVNIISLH PF2R_MOUSE 314 25 -
NLYKLASRCCGVNIISLH PF2R_RAT 314 25 -
NLYKLASQCCGVHVISLH PF2R_HUMAN 314 25 -
NLYVCTRRCCGVHVISLH PF2R_BOVIN 314 25 -

Motif 9 width=19
Element Seqn Id St Int Rpt
WELSSIKNSLKVAAISESP PF2R_MOUSE 333 1 -
WELSSIKNSLKVAAISESP PF2R_RAT 333 1 -
WELSSIKNSLKVAAISESP PF2R_HUMAN 333 1 -
WELSSIKDSLKVAAISDLP PF2R_BOVIN 333 1 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
SMNSSKQPVSPAAGLIANTTCQ PF2R_MOUSE 2 2 -
SINSSKQPASSAAGLIANTTCQ PF2R_RAT 2 2 -
SMNNSKQLVSPAAALLSNTTCQ PF2R_HUMAN 2 2 -
STNNSVQPVSPASELLSNTTCQ PF2R_SHEEP 2 2 -
STNSSIQPVSPESELLSNTTCQ PF2R_BOVIN 2 2 -

Motif 2 width=15
Element Seqn Id St Int Rpt
ENRLSVFFSIIFMTV PF2R_MOUSE 25 1 -
ENRLSVFFSIIFMTV PF2R_RAT 25 1 -
ENRLSVFFSVIFMTV PF2R_HUMAN 25 1 -
EEDLSISFSIIFMTV PF2R_SHEEP 25 1 -
EEDLSISFSIIFMTV PF2R_BOVIN 25 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
KAYQRFRQKSKASFLL PF2R_MOUSE 53 13 -
KAYQRFRRKSKASFLL PF2R_RAT 53 13 -
KAYQRFRQKSKASFLL PF2R_HUMAN 53 13 -
KAYQRFRQKYKSSFLL PF2R_SHEEP 53 13 -
KAYQRFRQKYKSSFLL PF2R_BOVIN 53 13 -

Motif 4 width=14
Element Seqn Id St Int Rpt
ILGHRDYQIQASRT PF2R_MOUSE 171 102 -
ILGHRDYQIQASRT PF2R_RAT 171 102 -
ILGHRDYKIQASRT PF2R_HUMAN 171 102 -
ILGHRDYKIQASRT PF2R_SHEEP 171 102 -
ILGHRDYKIQASRT PF2R_BOVIN 171 102 -

Motif 5 width=18
Element Seqn Id St Int Rpt
YNTEHIEDWEDRFYLLFF PF2R_MOUSE 188 3 -
YNTEHIEDWEDRFYLLFF PF2R_RAT 188 3 -
YNTEDIKDWEDRFYLLLF PF2R_HUMAN 188 3 -
YKTDQIKDWEDRFYLLLF PF2R_SHEEP 188 3 -
YKTDEIKDWEDRFYLLLF PF2R_BOVIN 188 3 -

Motif 6 width=17
Element Seqn Id St Int Rpt
LRVKFRSQQHRQGRSHH PF2R_MOUSE 228 22 -
LRVKFRSQQHRQGRSHH PF2R_RAT 228 22 -
LRVKFKSQQHRQGRSHH PF2R_HUMAN 228 22 -
LKVKFRSQQHRQGRSHH PF2R_SHEEP 228 22 -
LKVKFRSQQHRQGRSHH PF2R_BOVIN 228 22 -

Motif 7 width=17
Element Seqn Id St Int Rpt
IAINGNNSPVTCETTLF PF2R_MOUSE 272 27 -
IAINGNNSPVTCETTLF PF2R_RAT 272 27 -
IGINGNHSLETCETTLF PF2R_HUMAN 272 27 -
IGMNIQDFKDSCERTLF PF2R_SHEEP 272 27 -
IGMNIQDFKDSCERTLF PF2R_BOVIN 272 27 -

Motif 8 width=18
Element Seqn Id St Int Rpt
NLYKLASRCCGVNIISLH PF2R_MOUSE 314 25 -
NLYKLASRCCGVNIISLH PF2R_RAT 314 25 -
NLYKLASQCCGVHVISLH PF2R_HUMAN 314 25 -
NLYVCTRRCCGVHVISLH PF2R_SHEEP 314 25 -
NLYVCTRRCCGVHVISLH PF2R_BOVIN 314 25 -

Motif 9 width=19
Element Seqn Id St Int Rpt
WELSSIKNSLKVAAISESP PF2R_MOUSE 333 1 -
WELSSIKNSLKVAAISESP PF2R_RAT 333 1 -
WELSSIKNSLKVAAISESP PF2R_HUMAN 333 1 -
WELSSIKNSLKVAAISDLP PF2R_SHEEP 333 1 -
WELSSIKDSLKVAAISDLP PF2R_BOVIN 333 1 -