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PR00854

Identifier
PRSTNOIDDPR  [View Relations]  [View Alignment]  
Accession
PR00854
No. of Motifs
8
Creation Date
12-FEB-1998  (UPDATE 07-JUN-1999)
Title
Prostaglandin D receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01788 PROSTANOIDR; PR00428 PROSTAGLNDNR
INTERPRO; IPR000376
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL,
Prostanoids.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp239-251.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Prostanoids (prostaglandins (PG) and thromboxanes (TX)) mediate a wide
variety of actions and play important physiological roles in the cardio-
vascular and immune systems, and in pain sensation in peripheral systems
[6]. PGI2 and TXA2 have opposing actions, involving regulation of the
interaction of platelets with the vascular endothelium, while PGE2, PGI2
and PGD2 are powerful vasodilators and potentiate the action of various
autocoids to induce plasma extravasation and pain sensation. To date,
evidence for at least 5 classes of prostanoid receptor has been obtained.
However, identification of subtypes and their distribution is hampered by
expression of more than one receptor within a tissue, coupled with poor
selectivity of available agonists and antagonists. 
 
DP receptors have a limited distribution [6]. They mediate relaxation in
vascular, gastrointestinal and uterine smooth muscle in human and some 
other species; they inhibit platelet activation, and modify release of
hypothalamic and pituitary hormones [6]. The receptors activate adenylyl
cyclase through Gs [6].
 
PRSTNOIDDPR is an 8-element fingerprint that provides a signature for the
prostaglandin D receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas of 
the alignment that characterise the prostanoid DP receptors but distinguish
them from the rest of the prostanoid receptor family - motif 1 lies at the
N-terminus; motif 2 spans the first cytoplasmic loop; motif 3 lies in the
first external loop; motif 4 lies in the second external loop; motif 5 lies
in the third cytoplasmic loop; motif 6 resides at the C-terminal end of
TM domain 6; motif 7 spans the third external loop, leading into TM domain 7;
and motif 8 spans the C-terminal end of TM domain 7, leading into the 
C-terminal region. A single iteration on OWL29.6 was required to reach
convergence, no further sequences being identified beyond the starting set. 
Two partial matches were also found, both of which are prostanoid DP
receptor fragments.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
833333333
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
O35932        PD2R_HUMAN    PD2R_MOUSE    
Sequence Titles
O35932      PROSTAGLANDIN D2 RECEPTOR - Rattus norvegicus (Rat). 
PD2R_HUMAN Prostaglandin D2 receptor (Prostanoid DP receptor) (PGD receptor) - Homo sapiens (Human).
PD2R_MOUSE Prostaglandin D2 receptor (Prostanoid DP receptor) (PGD receptor) - Mus musculus (Mouse).
Scan History
OWL29_6    1  50   NSINGLE    
SPTR37_9f 2 7 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
YRCQTSTWVERGSSA MUSPDR2 5 5 -
YRCQAATWVERGSSA RNU92289 5 5 -
YRCQNTTSVEKGNSA I39153 6 6 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LARSGLGSCRPGPLHPPP MUSPDR2 40 20 -
LARSGLGSCRPGPLHPPP RNU92289 40 20 -
LARSGLGWCSRRPLRPLP I39153 41 20 -

Motif 3 width=12
Element Seqn Id St Int Rpt
LKELLPASGNQL MUSPDR2 92 34 -
LKELLPASGNQL RNU92289 92 34 -
LRVLAPALDNSL I39153 93 34 -

Motif 4 width=16
Element Seqn Id St Int Rpt
IQMIHKERSFSVIGFS MUSPDR2 184 80 -
IQMIHKKRSFSVIGFS RNU92289 184 80 -
IQMVHEEGSLSVLGYS I39153 185 80 -

Motif 5 width=24
Element Seqn Id St Int Rpt
PHRCSRDRAQSGSDYRHGSLHPLE MUSPDR2 235 35 -
PRRCSRDRAQSGSDYRHGSPNPLE RNU92289 235 35 -
PRSCTRDCAEPRADGREASPQPLE I39153 236 35 -

Motif 6 width=12
Element Seqn Id St Int Rpt
PLIYRAYYGAFK MUSPDR2 279 20 -
PLIYRAYYGAFK RNU92289 279 20 -
PVIYRAYYGAFK I39153 280 20 -

Motif 7 width=17
Element Seqn Id St Int Rpt
EGDSEDLQALRFLSVIS MUSPDR2 296 5 -
DGDSEDLQALRFLSVIS RNU92289 296 5 -
SEEAEDLRALRFLSVIS I39153 300 8 -

Motif 8 width=21
Element Seqn Id St Int Rpt
TSVFRMLFHKVFTRPLIYRNW MUSPDR2 324 11 -
TSVFRMLFHKAFTRPLIYRNW RNU92289 324 11 -
SPVFRIFFHKIFIRPLRYRSR I39153 328 11 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
YRCQAATWVERGSSA O35932 5 5 -
YRCQTSTWVERGSSA PD2R_MOUSE 5 5 -
YRCQNTTSVEKGNSA PD2R_HUMAN 6 6 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LARSGLGSCRPGPLHPPP O35932 40 20 -
LARSGLGSCRPGPLHPPP PD2R_MOUSE 40 20 -
LARSGLGWCSRRPLRPLP PD2R_HUMAN 41 20 -

Motif 3 width=12
Element Seqn Id St Int Rpt
LKELLPASGNQL O35932 92 34 -
LKELLPASGNQL PD2R_MOUSE 92 34 -
LRVLAPALDNSL PD2R_HUMAN 93 34 -

Motif 4 width=16
Element Seqn Id St Int Rpt
IQMIHKKRSFSVIGFS O35932 184 80 -
IQMIHKERSFSVIGFS PD2R_MOUSE 184 80 -
IQMVHEEGSLSVLGYS PD2R_HUMAN 185 80 -

Motif 5 width=24
Element Seqn Id St Int Rpt
PRRCSRDRAQSGSDYRHGSPNPLE O35932 235 35 -
PHRCSRDRAQSGSDYRHGSLHPLE PD2R_MOUSE 235 35 -
PRSCTRDCAEPRADGREASPQPLE PD2R_HUMAN 236 35 -

Motif 6 width=12
Element Seqn Id St Int Rpt
PLIYRAYYGAFK O35932 279 20 -
PLIYRAYYGAFK PD2R_MOUSE 279 20 -
PVIYRAYYGAFK PD2R_HUMAN 280 20 -

Motif 7 width=17
Element Seqn Id St Int Rpt
DGDSEDLQALRFLSVIS O35932 296 5 -
EGDSEDLQALRFLSVIS PD2R_MOUSE 296 5 -
SEEAEDLRALRFLSVIS PD2R_HUMAN 300 8 -

Motif 8 width=21
Element Seqn Id St Int Rpt
TSVFRMLFHKAFTRPLIYRNW O35932 324 11 -
TSVFRMLFHKVFTRPLIYRNW PD2R_MOUSE 324 11 -
SPVFRIFFHKIFIRPLRYRSR PD2R_HUMAN 328 11 -