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PR00847

Identifier
HYALURONDASE  [View Relations]  [View Alignment]  
Accession
PR00847
No. of Motifs
8
Creation Date
14-FEB-1998  (UPDATE 07-JUN-1999)
Title
Bee venom hyaluronidase signature
Database References
PRINTS; PR00846 GLHYDRLASE56
INTERPRO; IPR001329
Literature References
1. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence 
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
2. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
3. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
Nucleotide sequences of the Arb genes, which control beta-glucosidase
utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
coli Bgl operon and evidence for a new beta-glycohydrolase family
including enzymes from eubacteria, archaebacteria and humans.
J.BACTERIOL. 174 765-777 (1992).
 
5. GMACHL, M. AND KREIL, G.
Bee venom hyaluronidase is homologous to a membrane protein of mammalian
sperm. 
PROC.NATL.ACAD.SCI.U.S.A. 90 3569-3573 (1993). 

Documentation
O-Glycosyl hydrolases (EC  3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 56 encompasses a group of hyaluronidases (EC 3.2.1.35)
that includes venom hyaluronidases [5] and mammalian sperm surface proteins
(PH-20).
 
The venom of honeybees contains several biologically-active peptides and 
two enzymes, one of which is a hyaluronidase [5]. The amino acid sequence
of bee venom hyaluronidase contains 349 amino acids, and includes four
cysteines and a number of potential glycosylation sites [5]. The sequence
shows a high degree of similarity to PH-20, a membrane protein of mammalian
sperm involved in sperm-egg adhesion, supporting the view that hyaluronidases
play a role in fertilisation [5]. 
 
HYALURONDASE is an 8-element fingerprint that provides a signature for bee
venom hyaluronidases. The fingerprint was derived from an initial alignment
of 3 sequences: the motifs were drawn from conserved sections spanning 
virtually the full alignment length, focusing on those regions that 
characterise the venom hyaluronidases but distinguish them from other family
56 glycosyl hydrolases. A single iteration on OWL30.0 was required to reach
convergence, no further sequences being identified beyond the starting set.
A single partial match was also found, a closely related C.elegans sequence
that matches motifs 3 and 4.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
833333333
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
HUGA_APIME    HUGA_DOLMA    HUGA_VESVU    
Sequence Titles
HUGA_APIME  HYALURONOGLUCOSAMINIDASE PRECURSOR (EC 3.2.1.35) (HYALURONIDASE) (ALLERGEN API M 2) (API M II) - APIS MELLIFERA (HONEYBEE). 
HUGA_DOLMA HYALURONOGLUCOSAMINIDASE (EC 3.2.1.35) (HYALURONIDASE) (ALLERGEN DOL M 2) (DOL M II) - DOLICHOVESPULA MACULATA (WHITE-FACE HORNET) (BALD-FACED HORNET).
HUGA_VESVU HYALURONOGLUCOSAMINIDASE (EC 3.2.1.35) (HYALURONIDASE) (ALLERGEN VES V 2) (VES V II) - VESPULA VULGARIS (YELLOW JACKET) (WASP).
Scan History
OWL30_0    1  50   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
FMCHQYGLYFDEVT HUGA_DOLMA 17 17 -
FMCHQYDLYFDEVT HUGA_VESVU 17 17 -
FMCHKYGLRFEEVS HUGA_APIME 52 52 -

Motif 2 width=14
Element Seqn Id St Int Rpt
ISIFYDPGEFPALL HUGA_DOLMA 47 16 -
IAIFYDPGEFPALL HUGA_VESVU 47 16 -
IAILYDPGMFPALL HUGA_APIME 83 17 -

Motif 3 width=14
Element Seqn Id St Int Rpt
LDKTYPNRNFNGIG HUGA_DOLMA 91 30 -
LDKIYPNRNFSGIG HUGA_VESVU 91 30 -
LINQIPDKSFPGVG HUGA_APIME 127 30 -

Motif 4 width=16
Element Seqn Id St Int Rpt
RNEHPFWDKKMIELEA HUGA_DOLMA 134 29 -
RNEHPTWNKKMIELEA HUGA_VESVU 134 29 -
RREHPFWDDQRVEQEA HUGA_APIME 170 29 -

Motif 5 width=16
Element Seqn Id St Int Rpt
NMSPNNLVPDCDATAM HUGA_DOLMA 187 37 -
NMSPNNLVPECDVTAM HUGA_VESVU 187 37 -
NLTPNQPSAQCEATTM HUGA_APIME 223 37 -

Motif 6 width=17
Element Seqn Id St Int Rpt
RHELTPDQRVGLVQGRV HUGA_DOLMA 225 22 -
RQELTPDQRIGLVQGRV HUGA_VESVU 225 22 -
RWNLTSGERVGLVGGRV HUGA_APIME 261 22 -

Motif 7 width=17
Element Seqn Id St Int Rpt
YQDDTNTFLTETDVKKT HUGA_DOLMA 265 23 -
YQDETNTFLTETDVKKT HUGA_VESVU 265 23 -
YQDRRDTDLSRADLEAT HUGA_APIME 302 24 -

Motif 8 width=11
Element Seqn Id St Int Rpt
SSSDVNSLSKC HUGA_DOLMA 298 16 -
SSSDVNSLSKC HUGA_VESVU 298 16 -
SSDDINTKAKC HUGA_APIME 335 16 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
FMCHQYGLYFDEVT HUGA_DOLMA 17 17 -
FMCHQYDLYFDEVT HUGA_VESVU 17 17 -
FMCHKYGLRFEEVS HUGA_APIME 52 52 -

Motif 2 width=14
Element Seqn Id St Int Rpt
ISIFYDPGEFPALL HUGA_DOLMA 47 16 -
IAIFYDPGEFPALL HUGA_VESVU 47 16 -
IAILYDPGMFPALL HUGA_APIME 83 17 -

Motif 3 width=14
Element Seqn Id St Int Rpt
LDKTYPNRNFNGIG HUGA_DOLMA 91 30 -
LDKIYPNRNFSGIG HUGA_VESVU 91 30 -
LINQIPDKSFPGVG HUGA_APIME 127 30 -

Motif 4 width=16
Element Seqn Id St Int Rpt
RNEHPFWDKKMIELEA HUGA_DOLMA 134 29 -
RNEHPTWNKKMIELEA HUGA_VESVU 134 29 -
RREHPFWDDQRVEQEA HUGA_APIME 170 29 -

Motif 5 width=16
Element Seqn Id St Int Rpt
NMSPNNLVPDCDATAM HUGA_DOLMA 187 37 -
NMSPNNLVPECDVTAM HUGA_VESVU 187 37 -
NLTPNQPSAQCEATTM HUGA_APIME 223 37 -

Motif 6 width=17
Element Seqn Id St Int Rpt
RHELTPDQRVGLVQGRV HUGA_DOLMA 225 22 -
RQELTPDQRIGLVQGRV HUGA_VESVU 225 22 -
RWNLTSGERVGLVGGRV HUGA_APIME 261 22 -

Motif 7 width=17
Element Seqn Id St Int Rpt
YQDDTNTFLTETDVKKT HUGA_DOLMA 265 23 -
YQDETNTFLTETDVKKT HUGA_VESVU 265 23 -
YQDRRDTDLSRADLEAT HUGA_APIME 302 24 -

Motif 8 width=11
Element Seqn Id St Int Rpt
SSSDVNSLSKC HUGA_DOLMA 298 16 -
SSSDVNSLSKC HUGA_VESVU 298 16 -
SSDDINTKAKC HUGA_APIME 335 16 -