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PR00846

Identifier
GLHYDRLASE56  [View Relations]  [View Alignment]  
Accession
PR00846
No. of Motifs
6
Creation Date
15-FEB-1998  (UPDATE 07-JUN-1999)
Title
Glycosyl hydrolase family 56 signature
Database References
PRINTS; PR00847 HYALURONDASE; PR00848 SPERMPH20
INTERPRO; IPR001968
Literature References
1. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
2. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
3. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
Nucleotide sequences of the Arb genes, which control beta-glucosidase
utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
coli Bgl operon and evidence for a new beta-glycohydrolase family
including enzymes from eubacteria, archaebacteria and humans.
J.BACTERIOL. 174 765-777 (1992).
 
5. GMACHL, M. AND KREIL, G.
Bee venom hyaluronidase is homologous to a membrane protein of mammalian
sperm. 
PROC.NATL.ACAD.SCI.U.S.A. 90 3569-3573 (1993). 
 
6. LATHROP W.F., CARMICHAEL E.P., MYLES D.G., PRIMAKOFF P.
cDNA cloning reveals the molecular structure of a sperm surface protein, 
PH-20, involved in sperm-egg adhesion and the wide distribution of its
gene among mammals. 
J.CELL BIOL. 111 2939-2949 (1990). 

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 56 encompasses a group of hyaluronidases (EC 3.2.1.35)
that includes venom hyaluronidases [5] and mammalian sperm surface proteins
(PH-20) [6].
 
The venom of honeybees contains several biologically-active peptides and 
two enzymes, one of which is a hyaluronidase [5]. The amino acid sequence
of bee venom hyaluronidase contains 349 amino acids, and includes four
cysteines and a number of potential glycosylation sites [5]. The sequence
shows a high degree of similarity to PH-20, a membrane protein of mammalian 
sperm involved in sperm-egg adhesion, supporting the view that hyaluronidases
play a role in fertilisation [5]. 
 
PH-20 is required for sperm adhesion to the egg zona pellucida; it is
located on both the sperm plasma membrane and acrosomal membrane [6]. The
amino acid sequence of the mature protein contains 468 amino acids, and
includes six potential N-linked glycosylation sites and twelve cysteines,
eight of which are tightly clustered near the C-terminus [6].
 
GLHYDRLASE56 is a 6-element fingerprint that provides a signature for
family 56 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 13 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length. Two iterations on OWL30.0
were required to reach convergence, at which point a true set comprising
18 sequences was identified.
 
An update on SPTR37_9f identified a true set of 20 sequences.
Summary Information
20 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6202020202020
5000000
4000000
3000000
2000000
123456
True Positives
HUGA_APIME    HUGA_DOLMA    HUGA_VESVU    HYA1_CAVPO    
HYA1_HUMAN HYA1_MACFA HYA1_MOUSE HYA1_RABIT
O15177 O35631 O35632 O43820
O60540 O70229 Q12794 Q12891
Q22675 Q29152 Q62803 Q93013
Sequence Titles
HUGA_APIME  HYALURONOGLUCOSAMINIDASE PRECURSOR (EC 3.2.1.35) (HYALURONIDASE) (ALLERGEN API M 2) (API M II) - APIS MELLIFERA (HONEYBEE). 
HUGA_DOLMA HYALURONOGLUCOSAMINIDASE (EC 3.2.1.35) (HYALURONIDASE) (ALLERGEN DOL M 2) (DOL M II) - DOLICHOVESPULA MACULATA (WHITE-FACE HORNET) (BALD-FACED HORNET).
HUGA_VESVU HYALURONOGLUCOSAMINIDASE (EC 3.2.1.35) (HYALURONIDASE) (ALLERGEN VES V 2) (VES V II) - VESPULA VULGARIS (YELLOW JACKET) (WASP).
HYA1_CAVPO HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN PH-20) - CAVIA PORCELLUS (GUINEA PIG).
HYA1_HUMAN HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN PH-20) (SPERM ADHESION MOLECULE 1) - HOMO SAPIENS (HUMAN).
HYA1_MACFA HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN PH-20) - MACACA FASCICULARIS (CRAB EATING MACAQUE) (CYNOMOLGUS MONKEY).
HYA1_MOUSE HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN PH-20) - MUS MUSCULUS (MOUSE).
HYA1_RABIT HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN PH-20) - ORYCTOLAGUS CUNICULUS (RABBIT).
O15177 LYSOSOMAL HYALURONIDASE - HOMO SAPIENS (HUMAN).
O35631 HYALURONIDASE - MUS MUSCULUS (MOUSE).
O35632 HYALURONIDASE - MUS MUSCULUS (MOUSE).
O43820 SIMILAR TO HYALURONOGLUCOSAMINIDASE - HOMO SAPIENS (HUMAN).
O60540 HYALURONIDASE - HOMO SAPIENS (HUMAN).
O70229 HYALURONIDASE 1 (HYALURONOGLUCOSAMINIDASE 1) - MUS MUSCULUS (MOUSE).
Q12794 TUMOR SUPPRESSOR (LUCA-1) - HOMO SAPIENS (HUMAN).
Q12891 LYSOSOMAL HYALURONIDASE (PH-20 HOMOLOG) (LUCA-2) (LUCA2) (PH-20 HOMOLOGY) - HOMO SAPIENS (HUMAN).
Q22675 T22C8.2 PROTEIN - CAENORHABDITIS ELEGANS.
Q29152 PROBABLE HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN PH-20) - VULPES VULPES (RED FOX).
Q62803 PROBABLE HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN PH-20) (2B1 ANTIGEN PROTEIN) - RATTUS NORVEGICUS (RAT).
Q93013 HYALURONOGLUCOSAMINIDASE 1 (LUCA-1) (TUMOR SUPPRESSOR LUCA-1) - HOMO SAPIENS (HUMAN).
Scan History
OWL30_0    2  40   NSINGLE    
SPTR37_9f 2 21 NSINGLE
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
LLWVWNAPTEFCI HYA1_CAVPO 48 48 -
FLWIWNVPTERCV HYA1_MOUSE 49 49 -
FLWAWNAPSEFCL HYA1_HUMAN 49 49 -
FLWAWNAPSEFCL HYA1_MACFA 49 49 -
FLWAWNAPTEFCL HYA1_RABIT 49 49 -
FVVAWDVPTQDCG AC002455 36 36 -
FTTVWNANTQWCL JC5584 32 32 -
FTTVWNANTQWCL HSU03056 32 32 -
TDVVWMVPSWTCK CET22C81 36 36 -
FVVAWNVPTQECA MMAJ0059 36 36 -
FNVYWNVPTFMCH HUGA_APIME 43 43 -
FNIYWNVPTFMCH HUGA_VESVU 8 8 -
FNIYWNVPTFMCH HUGA_DOLMA 8 8 -

Motif 2 width=15
Element Seqn Id St Int Rpt
KNGGLPQMGDLEAHL CET22C81 97 48 -
VHGGVPQNGSLCAHL MMAJ0059 96 47 -
VHGGVPQNVSLWAHR AC002455 96 47 -
VFGGLPQNASLIAHL JC5584 92 47 -
VFGGLPQNASLIAHL HSU03056 92 47 -
VHGRIPQLGPLQQHL HYA1_RABIT 110 48 -
VHGGIPQKVSLQDHL HYA1_MACFA 110 48 -
VNGGIPQKISLQDHL HYA1_HUMAN 110 48 -
HYGGIPQRGDYQAHL HYA1_MOUSE 109 47 -
VHGGLPQLMNLQQHL HYA1_CAVPO 109 48 -
RNGGVPQEGNITIHL HUGA_DOLMA 70 49 -
RNGGVPQEGNITIHL HUGA_VESVU 70 49 -
RNGGVPQLGNLTKHL HUGA_APIME 106 50 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GIGVIDFERWRPIFRQNW HUGA_VESVU 102 17 -
GIAVIDIEEFRPMWELSW CET22C81 129 17 -
GLAVIDWEAWRPRWAFNW HSU03056 124 17 -
GLAVIDWEAWRPRWAFNW JC5584 124 17 -
GLAVIDWEDWRPVWVRNW AC002455 128 17 -
GLAVIDWEEWRPVWVRNW MMAJ0059 128 17 -
GVGVIDFESWRPIFRQNW HUGA_APIME 138 17 -
GLAVIDWEEWLPTWLRNW HYA1_RABIT 141 16 -
GMAVIDWEEWRPTWARNW HYA1_MACFA 141 16 -
GMAVIDWEEWRPTWARNW HYA1_HUMAN 141 16 -
GLAIIDWEEWRPTWLRNW HYA1_MOUSE 140 16 -
GLAVIDWEEWRPTWTRNW HYA1_CAVPO 140 16 -
GIGVIDFERWRPIFRQNW HUGA_DOLMA 102 17 -

Motif 4 width=26
Element Seqn Id St Int Rpt
FMEETLKLAKKTRKQADWGYYGYPYC HUGA_DOLMA 160 40 -
FFIETLRLGKRLRPNAKWGYYLFPKC CET22C81 187 40 -
WMAGTLQLGGALRPRGLWGFYGFPDC HSU03056 182 40 -
FMEETLKAAKRMRPAANWGYYAYPYC HUGA_APIME 196 40 -
WMAGTLQLGRALRPRGLWGFYGFPDC JC5584 182 40 -
FMLETLRYVKAVRPRHLWGFYLFPDC AC002455 186 40 -
FMLNTLRLRQGSQTQHLWGFYLFPDC MMAJ0059 186 40 -
FMLETLKLGKSLRPSSLWGYYLFPDC HYA1_CAVPO 198 40 -
FMEGTLHLGKFLRPNQLWGYYLFPDC HYA1_MOUSE 198 40 -
FLVETIKLGKLLRPNHLWGYYLFPDC HYA1_HUMAN 199 40 -
FMLETIKLGRSLRPNHLWGYYLFPDC HYA1_MACFA 199 40 -
FMEETLKLGRLLRPNHLWGYYLFPDC HYA1_RABIT 199 40 -
FMEETLKLAKKTRKQADWGYYGYPYC HUGA_VESVU 160 40 -

Motif 5 width=15
Element Seqn Id St Int Rpt
NDNLKWLWKASTGLY HYA1_MOUSE 245 21 -
NDDLSWLWKESTALF HYA1_RABIT 247 22 -
NDDLSWLWNESTALY HYA1_MACFA 246 21 -
NDDLSWLWNESTALY HYA1_HUMAN 246 21 -
NNDLQWLWNDSTALY HYA1_CAVPO 245 21 -
NDKMSWLFNNQNVLL HUGA_DOLMA 205 19 -
NDKMSWLFNNQNVLL HUGA_VESVU 205 19 -
NDKMSWLFESEDVLL HUGA_APIME 241 19 -
NDQLAWLWAESTALF MMAJ0059 235 23 -
NDQLAWLWAESTALF AC002455 235 23 -
NDQLGWLWGQSRALY JC5584 229 21 -
NDQLGWLWGQSRALY HSU03056 229 21 -
NDNLHWLWGESTALF CET22C81 232 19 -

Motif 6 width=14
Element Seqn Id St Int Rpt
CQYLKDYLTRLLVP AC002455 340 90 -
CLQFREYLNNELGP HUGA_APIME 345 89 -
CKRLQDYLLTVLGP HUGA_VESVU 308 88 -
CKRLREYLLTVLGP HUGA_DOLMA 308 88 -
CIGLENYMKGTLLP HYA1_CAVPO 351 91 -
CPILHKYMQTTLNP HYA1_MOUSE 351 91 -
CLLLDNYMETILNP HYA1_HUMAN 351 90 -
CLLLDTYMETILNP HYA1_MACFA 351 90 -
CLHLDNYMKTILNP HYA1_RABIT 355 93 -
CGSLQTYVDNTLGP CET22C81 337 90 -
CQYLKNYLTQLLVP MMAJ0059 339 89 -
CQAIKEYMDTTLGP HSU03056 333 89 -
CQAIKEYMDTTLGP JC5584 333 89 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
FVVAWNVPTQECA O35631 36 36 -
FVVAWNVPTQECA O35632 36 36 -
FLWAWNAPTEFCL HYA1_RABIT 49 49 -
FLWAWNAPSEFCL HYA1_MACFA 49 49 -
FLWAWNAPSEFCL HYA1_HUMAN 49 49 -
FVVAWDVPTQDCG O15177 36 36 -
FVVAWDVPTQDCG Q12891 36 36 -
FVWVWNVPTEACV Q62803 49 49 -
FTTVWNANTQWCL Q93013 32 32 -
FTTVWNANTQWCL Q12794 32 32 -
FLWGWNAPTELCA Q29152 49 49 -
FLWIWNVPTERCV HYA1_MOUSE 49 49 -
LLWVWNAPTEFCI HYA1_CAVPO 48 48 -
FITVWNGDTHWCL O70229 60 60 -
FSVLWNVPSAHCE O43820 31 31 -
FSVLWNVPSAHCE O60540 31 31 -
TDVVWMVPSWTCK Q22675 36 36 -
FNIYWNVPTFMCH HUGA_DOLMA 8 8 -
FNIYWNVPTFMCH HUGA_VESVU 8 8 -
FNVYWNVPTFMCH HUGA_APIME 43 43 -

Motif 2 width=15
Element Seqn Id St Int Rpt
VHGGVPQNGSLCAHL O35631 96 47 -
VHGGVPQNGSLCAHL O35632 96 47 -
VHGRIPQLGPLQQHL HYA1_RABIT 110 48 -
VHGGIPQKVSLQDHL HYA1_MACFA 110 48 -
VNGGIPQKISLQDHL HYA1_HUMAN 110 48 -
VHGGVPQNVSLWAHR O15177 96 47 -
VHGGVPQNVSLWAHR Q12891 96 47 -
HHGGIPQKGDLTTHL Q62803 109 47 -
VFGGLPQNASLIAHL Q93013 92 47 -
VFGGLPQNASLIAHL Q12794 92 47 -
VNGGIPQLGSLKKHL Q29152 110 48 -
HYGGIPQRGDYQAHL HYA1_MOUSE 109 47 -
VHGGLPQLMNLQQHL HYA1_CAVPO 109 48 -
VFGGLPQNASLVTHL O70229 120 47 -
HNGGIPQALPLDRHL O43820 91 47 -
HNGGIPQALPLDRHL O60540 91 47 -
KNGGLPQMGDLEAHL Q22675 97 48 -
RNGGVPQEGNITIHL HUGA_DOLMA 70 49 -
RNGGVPQEGNITIHL HUGA_VESVU 70 49 -
RNGGVPQLGNLTKHL HUGA_APIME 106 50 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GLAVIDWEEWRPVWVRNW O35631 128 17 -
GLAVIDWEEWRPVWVRNW O35632 128 17 -
GLAVIDWEEWLPTWLRNW HYA1_RABIT 141 16 -
GMAVIDWEEWRPTWARNW HYA1_MACFA 141 16 -
GMAVIDWEEWRPTWARNW HYA1_HUMAN 141 16 -
GLAVIDWEDWRPVWVRNW O15177 128 17 -
GLAVIDWEDWRPVWVRNW Q12891 128 17 -
GLAIIDWEEWRPTWMRNW Q62803 140 16 -
GLAVIDWEAWRPRWAFNW Q93013 124 17 -
GLAVIDWEAWRPRWAFNW Q12794 124 17 -
GLAVIDWDSWRPNWARNW Q29152 141 16 -
GLAIIDWEEWRPTWLRNW HYA1_MOUSE 140 16 -
GLAVIDWEEWRPTWTRNW HYA1_CAVPO 140 16 -
GLAVIDWEAWRPRWAFNW O70229 152 17 -
GPAVLDWEEWCPLWAGNW O43820 122 16 -
GPAVLDWEEWCPLWAGNW O60540 122 16 -
GIAVIDIEEFRPMWELSW Q22675 129 17 -
GIGVIDFERWRPIFRQNW HUGA_DOLMA 102 17 -
GIGVIDFERWRPIFRQNW HUGA_VESVU 102 17 -
GVGVIDFESWRPIFRQNW HUGA_APIME 138 17 -

Motif 4 width=26
Element Seqn Id St Int Rpt
FMLNTLRLRQGSQTQHLWGFYLFPDC O35631 186 40 -
FMLNTLRLRQGSQTQHLWGFYLFPDC O35632 186 40 -
FMEETLKLGRLLRPNHLWGYYLFPDC HYA1_RABIT 199 40 -
FMLETIKLGRSLRPNHLWGYYLFPDC HYA1_MACFA 199 40 -
FLVETIKLGKLLRPNHLWGYYLFPDC HYA1_HUMAN 199 40 -
FMLETLRYVKAVRPRHLWGFYLFPDC O15177 186 40 -
FMLETLRYVKAVRPRHLWGFYLFPDC Q12891 186 40 -
FMEGTLKLGKHIRPKHLWGFYLFPDC Q62803 198 40 -
WMAGTLQLGRALRPRGLWGFYGFPDC Q93013 182 40 -
WMAGTLQLGGALRPRGLWGFYGFPDC Q12794 182 40 -
FMQETLKLGKFLRPNYLWGFYLYPDC Q29152 199 40 -
FMEGTLHLGKFLRPNQLWGYYLFPDC HYA1_MOUSE 198 40 -
FMLETLKLGKSLRPSSLWGYYLFPDC HYA1_CAVPO 198 40 -
WMAGTLQLGQVLRPRGLWGYYGFPDC O70229 210 40 -
LMEDTLRVAQALRPHGLWGFYHYPAC O43820 180 40 -
LMEDTLRVAQALRPHGLWGFYHYPAC O60540 180 40 -
FFIETLRLGKRLRPNAKWGYYLFPKC Q22675 187 40 -
FMEETLKLAKKTRKQADWGYYGYPYC HUGA_DOLMA 160 40 -
FMEETLKLAKKTRKQADWGYYGYPYC HUGA_VESVU 160 40 -
FMEETLKAAKRMRPAANWGYYAYPYC HUGA_APIME 196 40 -

Motif 5 width=15
Element Seqn Id St Int Rpt
NDQLAWLWAESTALF O35631 235 23 -
NDQLAWLWAESTALF O35632 235 23 -
NDDLSWLWKESTALF HYA1_RABIT 247 22 -
NDDLSWLWNESTALY HYA1_MACFA 246 21 -
NDDLSWLWNESTALY HYA1_HUMAN 246 21 -
NDQLAWLWAESTALF O15177 235 23 -
NDQLAWLWAESTALF Q12891 235 23 -
NDDLDWLWKESTGLY Q62803 245 21 -
NDQLGWLWGQSRALY Q93013 229 21 -
NDQLGWLWGQSRALY Q12794 229 21 -
NDEIDWLWKESTALF Q29152 246 21 -
NDNLKWLWKASTGLY HYA1_MOUSE 245 21 -
NNDLQWLWNDSTALY HYA1_CAVPO 245 21 -
NDQLGWLWNQSYALY O70229 257 21 -
NTQLHWLWAASSALF O43820 228 22 -
NTQLHWLWAASSALF O60540 228 22 -
NDNLHWLWGESTALF Q22675 232 19 -
NDKMSWLFNNQNVLL HUGA_DOLMA 205 19 -
NDKMSWLFNNQNVLL HUGA_VESVU 205 19 -
NDKMSWLFESEDVLL HUGA_APIME 241 19 -

Motif 6 width=14
Element Seqn Id St Int Rpt
CQYLKNYLTQLLVP O35631 339 89 -
CQYLKNYLTQLLVP O35632 340 90 -
CLHLDNYMKTILNP HYA1_RABIT 355 93 -
CLLLDTYMETILNP HYA1_MACFA 351 90 -
CLLLDNYMETILNP HYA1_HUMAN 351 90 -
CQYLKDYLTRLLVP O15177 339 89 -
CQYLKDYLTRLLVP Q12891 340 90 -
CPILRQYMKTTLNP Q62803 351 91 -
CQAIKEYMDTTLGP Q93013 333 89 -
CQAIKEYMDTTLGP Q12794 333 89 -
CTELDTYIKNKLNP Q29152 352 91 -
CPILHKYMQTTLNP HYA1_MOUSE 351 91 -
CIGLENYMKGTLLP HYA1_CAVPO 351 91 -
CQAIKAYMDSTLGP O70229 361 89 -
CWHLHDYLVDTLGP O43820 331 88 -
CWHLHDYLVDTLGP O60540 331 88 -
CGSLQTYVDNTLGP Q22675 337 90 -
CKRLREYLLTVLGP HUGA_DOLMA 308 88 -
CKRLQDYLLTVLGP HUGA_VESVU 308 88 -
CLQFREYLNNELGP HUGA_APIME 345 89 -