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PR00843

Identifier
GLHYDRLASE30  [View Relations]  [View Alignment]  
Accession
PR00843
No. of Motifs
6
Creation Date
13-FEB-1998  (UPDATE 07-JUN-1999)
Title
Glycosyl hydrolase family 30 signature
Database References

INTERPRO; IPR001139
Literature References
1. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
2. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
3. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
Nucleotide sequences of the Arb genes, which control beta-glucosidase
utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
coli Bgl operon and evidence for a new beta-glycohydrolase family
including enzymes from eubacteria, archaebacteria and humans.
J.BACTERIOL. 174 765-777 (1992).
 
5. DINUR, T., OSIECKI, K.M., LEGLER, G., GATT, S., DESNICK, R.J.
AND GRABOWSKI, G.A.
Human acid beta-glucosidase: isolation and amino acid sequence of a peptide
containing the catalytic site. 
PROC.NATL.ACAD.SCI.U.S.A. 83 1660-1664 (1986). 
 
6. WINFIELD, S.L., TAYEBI, N., MARTIN, B.M., GINNS, E.I. AND SIDRANSKY, E.
Identification of three additional genes contiguous to the glucocerebrosidase 
locus on chromosome 1q21: Implications for Gaucher disease.
GENOME RES. 7 1020-1026 (1997).
 
7. IWASAWA, K., IDA, H. AND ETO, Y.
Differences in origin of the 1448C mutation in patients with Gaucher disease.
ACTA PAEDIATR.JPN. 39 451-453 (1997).

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 30 encompasses the mammalian glucosyl-ceramidases (EC
3.2.1.45).
 
Human acid beta-glucosidase (D-glucosyl-N-acylsphingosine glucohydrolase),
cleaves the glucosidic bonds of glucosylceramide and synthetic beta-
glucosides [5]. Any one of over 50 different mutations in the gene of
glucocerebrosidase have been found to affect activity of this hydrolase,
producing variants of Gaucher disease, the most prevalent lysosomal
storage disease [5,7]. 
 
GLHYDRLASE30 is a 6-element fingerprint that provides a signature for
family 30 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions 
spanning the N-terminal half of the alignment - motifs 1 and 4 each include
potential glycosylation sites; and motif 5 encodes a putative proton donor
site. A single iteration on OWL29.6 was required to reach convergence, no
further sequences being identified beyond the starting set. Two partial
matches were found: CEF11E6 is a fragment that lacks the portion of sequence
bearing motifs 5 and 6; and I67792, a putative glucosylceramidase that
matches motifs 4, 5 and 6.
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6555555
5000000
4000000
3000000
2000000
123456
True Positives
GLCM_HUMAN    GLCM_MOUSE    O16580        O16581        
Q16545
Sequence Titles
GLCM_HUMAN  GLUCOSYLCERAMIDASE PRECURSOR (EC 3.2.1.45) (BETA-GLUCOCEREBROSIDASE) (ACID BETA-GLUCOSIDASE) (D-GLUCOSYL-N-ACYLSPHINGOSINE GLUCOHYDROLASE) (ALGLUCERASE) (IMIGLUCERASE) - HOMO SAPIENS (HUMAN). 
GLCM_MOUSE GLUCOSYLCERAMIDASE PRECURSOR (EC 3.2.1.45) (BETA-GLUCOCEREBROSIDASE) (ACID BETA-GLUCOSIDASE) (D-GLUCOSYL-N-ACYLSPHINGOSINE GLUCOHYDROLASE) - MUS MUSCULUS (MOUSE).
O16580 C33C12.3 PROTEIN - CAENORHABDITIS ELEGANS.
O16581 C33C12.8 PROTEIN - CAENORHABDITIS ELEGANS.
Q16545 GLUCOCEREBROSIDASE PRECURSOR - HOMO SAPIENS (HUMAN).
Scan History
OWL29_6    1  50   NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
TGIVCVCNITYCDEIPDINL CELC33C125 78 78 -
TGTVCVCSLDSCDEIPPLDI CELC33C124 33 33 -
SSVVCVCNATYCDSFDPPTF GLCM_HUMAN 51 51 -
SSVVCVCNATYCDSFDPPTF HUMGCBL 31 31 -
SSVVCVCNASYCDSLDPVTL GLCM_MOUSE 31 31 -

Motif 2 width=21
Element Seqn Id St Int Rpt
TIDSSKKYQTIQGFGSTFSDA CELC33C124 88 35 -
TIDSSKTYQTIQGFGSTFSDA CELC33C125 133 35 -
TLQPEQKFQKVKGFGGAMTDA HUMGCBL 87 36 -
TLQPEKKFQKVKGFGGAMTDA GLCM_MOUSE 87 36 -
TLQPEQKFQKVKGFGGAMTDA GLCM_HUMAN 107 36 -

Motif 3 width=27
Element Seqn Id St Int Rpt
LLLKSYFSEEGIGYNIIRVPMASCDFS GLCM_HUMAN 142 14 -
LLLKSYFSEEGIGYNIIRVPMASCDFS HUMGCBL 122 14 -
TILRQYFSDSGLNLQFGRVPIASNDFS CELC33C125 168 14 -
LIMKQYFSDTGLNLQFGRVPIASTDFS CELC33C124 123 14 -
LLLRSYFSTNGIEYNIIRVPMASCDFS GLCM_MOUSE 122 14 -

Motif 4 width=29
Element Seqn Id St Int Rpt
RVYTYADTPNDFQLSNFSLPEEDTKLKIP GLCM_MOUSE 150 1 -
RTYTYADTPDDFQLHNFSLPEEDTKLKIP HUMGCBL 150 1 -
RVYTYDDNLEDYNMAHFSLQREDYQWKIP CELC33C125 196 1 -
RVYSYNDVANDYSMQNFNLTKEDFQWKIP CELC33C124 151 1 -
RTYTYADTPDDFQLHNFSLPEEDTKLKIP GLCM_HUMAN 170 1 -

Motif 5 width=18
Element Seqn Id St Int Rpt
DTYHKSYVTYILHFLEEY CELC33C125 267 42 -
DIYHQTWARYFVKFLDAY HUMGCBL 222 43 -
DIFHQTWANYFVKFLDAY GLCM_MOUSE 222 43 -
DIYHQTWARYFVKFLDAY GLCM_HUMAN 242 43 -
DNYHQAYAKYFVRFLEEY CELC33C124 222 42 -

Motif 6 width=23
Element Seqn Id St Int Rpt
VKLLMLDDQRLLLPRWAEVVLSD GLCM_MOUSE 294 54 -
VRLLMLDDQRLLLPHWAKVVLTD HUMGCBL 295 55 -
VKILILDDNRGNLPKWADTVLND CELC33C125 341 56 -
VKLLILDDNRGNLPKWADTVLND CELC33C124 296 56 -
VRLLMLDDQRLLLPHWAKVVLTD GLCM_HUMAN 315 55 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
SSVVCVCNATYCDSFDPPTF GLCM_HUMAN 51 51 -
SSVVCVCNATYCDSFDPPTF Q16545 51 51 -
SSVVCVCNASYCDSLDPVTL GLCM_MOUSE 31 31 -
TGTVCVCSLDSCDEIPPLDI O16580 33 33 -
TGIVCVCNITYCDEIPDINL O16581 78 78 -

Motif 2 width=21
Element Seqn Id St Int Rpt
TLQPEQKFQKVKGFGGAMTDA GLCM_HUMAN 107 36 -
TLQPEQKFQKVKGFGGAMTDA Q16545 107 36 -
TLQPEKKFQKVKGFGGAMTDA GLCM_MOUSE 87 36 -
TIDSSKKYQTIQGFGSTFSDA O16580 88 35 -
TIDSSKTYQTIQGFGSTFSDA O16581 133 35 -

Motif 3 width=27
Element Seqn Id St Int Rpt
LLLKSYFSEEGIGYNIIRVPMASCDFS GLCM_HUMAN 142 14 -
LLLKSYFSEEGIGYNIIRVPMASCDFS Q16545 142 14 -
LLLRSYFSTNGIEYNIIRVPMASCDFS GLCM_MOUSE 122 14 -
LIMKQYFSDTGLNLQFGRVPIASTDFS O16580 123 14 -
TILRQYFSDSGLNLQFGRVPIASNDFS O16581 168 14 -

Motif 4 width=29
Element Seqn Id St Int Rpt
RTYTYADTPDDFQLHNFSLPEEDTKLKIP GLCM_HUMAN 170 1 -
RTYTYADTPDDFQLHNFSLPEEDTKLKIP Q16545 170 1 -
RVYTYADTPNDFQLSNFSLPEEDTKLKIP GLCM_MOUSE 150 1 -
RVYSYNDVANDYSMQNFNLTKEDFQWKIP O16580 151 1 -
RVYTYDDNLEDYNMAHFSLQREDYQWKIP O16581 196 1 -

Motif 5 width=18
Element Seqn Id St Int Rpt
DIYHQTWARYFVKFLDAY GLCM_HUMAN 242 43 -
DIYHQTWARYFVKFLDAY Q16545 242 43 -
DIFHQTWANYFVKFLDAY GLCM_MOUSE 222 43 -
DNYHQAYAKYFVRFLEEY O16580 222 42 -
DTYHKSYVTYILHFLEEY O16581 267 42 -

Motif 6 width=23
Element Seqn Id St Int Rpt
VRLLMLDDQRLLLPHWAKVVLTD GLCM_HUMAN 315 55 -
VRLLMLDDQRLLLPHWAKVVLTD Q16545 315 55 -
VKLLMLDDQRLLLPRWAEVVLSD GLCM_MOUSE 294 54 -
VKLLILDDNRGNLPKWADTVLND O16580 296 56 -
VKILILDDNRGNLPKWADTVLND O16581 341 56 -