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PR00839

Identifier
V8PROTEASE  [View Relations]  [View Alignment]  
Accession
PR00839
No. of Motifs
6
Creation Date
18-MAR-1998  (UPDATE 14-JUN-1999)
Title
V8 serine protease family signature
Database References

PROSITE; PS00672 V8_HIS; PS00673 V8_SER
BLOCKS; BL00672
INTERPRO; IPR000126
Literature References
1. DANCER, S.J., GARRATT, R., SALDANHA, J., JHOTI, H. AND EVANS, R.      
The epidermolytic toxins are serine proteases.
FEBS LETT. 268 129-132 (1990).
 
2. BAILEY, C.J. AND SMITH, T.P.      
The reactive serine residue of epidermolytic toxin-A.
BIOCHEM.J. 269 535-537 (1990).
 
3. RAWLINGS, N.D. AND BARRETT, A.J.      
Families of serine peptidases.
METHODS ENZYMOL. 244 19-61 (1994).
 
4. SVENDSEN, I. AND BREDDAM, K.      
Isolation and amino acid sequence of a glutamic acid-specific 
endopeptidase from Bacillus licheniformis.
EUR.J.BIOCHEMISTRY 204 165-171 (1992).
 
5. SLOMA, A., RUDOLPH, C.F., RUFO, G.A. JR., SULLIVAN, B.J., THERIAULT, K.A.,
ALLY, D. AND PERO, J.      
Gene encoding a novel extracellular metalloprotease in Bacillus subtilis.
J.BACTERIOL. 172 1024-1029 (1990).

Documentation
In a number of prokaryotic proteases the catalytic activity is provided by
a charge relay system analagous to that of the trypsin family of serine
proteases, but which probably evolved by independent convergent evolution
[1,2]. The sequences around the catalytic residues (Asp, Ser and His) are 
completely different from those of the corresponding residues in trypsin
serine proteases. 
 
These proteins belong to family S2B of the peptidase classification system
[3]. Members include S.aureus V8 protease, which preferentially cleaves
peptide bonds C-terminal to Asp and Glu residues; B.licheniformis glutamate-
specific endopeptidase [4], which also cleaves on the C-terminal side of 
acidic residues, but with strong preference for glutamate; B.subtilis
extracellular "metalloprotease" [5]; and S.aureus exfoliative (or 
epidermolytic) toxins A and B, which cause impetigous diseases (also known
as Staphylococcal scalded skin syndrome).
 
V8PROTEASE is a 6-element fingerprint that provides a signature for the
V8 family of serine proteases. The fingerprint was derived from an initial
alignment of 10 sequences: the motifs were drawn from short conserved
regions spanning virtually the full alignment length - motif 2 includes the
region encoded by PROSITE pattern V8_HIS (PS00672), which contains the
active site His; and motif 5 spans the region encoded by PROSITE pattern
V8_SER (PS00673), which includes the active site serine. A single iteration
on OWL30.0 was required to reach convergence, no further sequences being 
identified beyond the starting set. Several partial matches were also found, 
all of which appear to be related proteases that match motif 5, and most of
which also match motif 6.
 
An update on SPTR37_9f identified a true set of 10 sequences, and 8
partial matches.
Summary Information
  10 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
8 codes involving 2 elements
Composite Feature Index
6101010101010
5000000
4000000
3000000
2020176
123456
True Positives
ETA_STAAU     ETB_STAAU     GSEP_BACLI    MPR_BACSU     
P76176 Q04186 Q47809 Q53781
Q53782 STSP_STAAU
True Positive Partials
Codes involving 2 elements
O82261 P72780 P73940 Q00344
Q06502 Q50335 Q83017 RPOA_LELV
Sequence Titles
ETA_STAAU   Exfoliative toxin A precursor (EC 3.4.21.-) (Epidermolytic toxin A) - Staphylococcus aureus. 
ETB_STAAU Exfoliative toxin B precursor (EC 3.4.21.-) (Epidermolytic toxin B) - Staphylococcus aureus.
GSEP_BACLI GLUTAMYL ENDOPEPTIDASE PRECURSOR (EC 3.4.21.19) (GLUTAMATE SPECIFIC ENDOPEPTIDASE) (GSE) - BACILLUS LICHENIFORMIS.
MPR_BACSU EXTRACELLULAR METALLOPROTEASE PRECURSOR (EC 3.4.21.-) - BACILLUS SUBTILIS.
P76176 FROM BASES 1663181 TO 1676139 (SECTION 145 OF 400) OF THE COMPLETE GENOME (SECTION 145 OF 400) - ESCHERICHIA COLI.
Q04186 GLUTAMIC ACID SPECIFIC PROTEASE PREPROPEPTIDE (EC 3.4.21.19) - Staphylococcus aureus.
Q47809 STAPHYLOCOCCAL SERINE PROTEINASE HOMOLOGUE - ENTEROCOCCUS FAECALIS (STREPTOCOCCUS FAECALIS).
Q53781 NOVEL ANTIGEN - STAPHYLOCOCCUS AUREUS.
Q53782 NOVEL ANTIGEN - STAPHYLOCOCCUS AUREUS.
STSP_STAAU Glutamyl endopeptidase precursor (EC 3.4.21.19) (Staphylococcal serine proteinase) (V8 proteinase) (Endoproteinase GLU-C) - Staphylococcus aureus.

O82261 F17A22.33 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
P72780 PROTEASE HHOA - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P73940 PROTEASE HHOB - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
Q00344 ALP1 - COCHLIOBOLUS CARBONUM (BIPOLARIS ZEICOLA).
Q06502 POLYPROTEIN - LACTATE DEHYDROGENASE-ELEVATING VIRUS (LDV).
Q50335 PUTATIVE LIPOPROTEIN - MYCOPLASMA PNEUMONIAE.
Q83017 POLYPROTEIN 1A - LACTATE DEHYDROGENASE-ELEVATING VIRUS (LDV).
RPOA_LELV POL POLYPROTEIN (ORF1A/1B) [CONTAINS: RNA-DIRECTED RNA POLYMERASE (EC 2.7.7.48); HELICASE; PROTEASE (EC 3.4.21.-)] - LELYSTAD VIRUS (LV) (PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS).
Scan History
OWL30_0    1  100  NSINGLE    
SPTR37_9f 2 70 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
KVDEKDRQKYPYNTIGNV ETA_STAAU 70 70 -
RTRISSTTSFPYRATVQL MPR_BACSU 101 101 -
YTHITDNARSPYNSVGTV ETB_STAAU 56 56 -
RTRVTNTTAYPYRAIVHI GSEP_BACLI 102 102 -
RHQITDTTNGHYAPVTYI STSP_STAAU 76 76 -
VTQVKDTNNFPYNGVVSF SAU63529 40 40 -
VTKIQDTNIFPYTGVVAF SAU60589 40 40 -
RHQITDTTNGHYAPVTYI S21758 76 76 -
RVPVNDTTQSPWDAVGQL H64915 44 44 -
RQEVADTTEAPFASIGRI S25140 55 55 -

Motif 2 width=18
Element Seqn Id St Int Rpt
ATGFVVGKNTILTNKHVS SAU60589 60 2 -
ASGVVVGKDTLLTNKHVV S21758 104 10 -
ATGFVIGKNTIITNKHVS SAU63529 60 2 -
CTATLIAPNLALTAGHCL H64915 69 7 -
GTGFVVGTNTIVTNNHVA S25140 85 12 -
ASGVVVGKDTLLTNKHVV STSP_STAAU 104 10 -
CTGWMIGPKTVATAGHCI GSEP_BACLI 126 6 -
ATGVLIGKNTIVTNYHVA ETB_STAAU 81 7 -
CTGFLVNPNTVVTAGHCV MPR_BACSU 131 12 -
ATGVLIGKNTVLTNRHIA ETA_STAAU 95 7 -

Motif 3 width=13
Element Seqn Id St Int Rpt
FYPGRDGSATPFG S25140 121 18 -
NTDDNGNTETPYG ETA_STAAU 129 16 -
AAPGRNGSSYPYG MPR_BACSU 161 12 -
RDAEKNEFPTPYG ETB_STAAU 116 17 -
VSPGRNGTSYPYG GSEP_BACLI 157 13 -
KAFPSAINQDNYP STSP_STAAU 132 10 -
KAFPSAINQDNYP S21758 132 10 -
HPNGDKGNGGIYK SAU63529 89 11 -
HPNSDKGNGGIYS SAU60589 89 11 -
KGLWRYEIHDIEG H64915 106 19 -

Motif 4 width=14
Element Seqn Id St Int Rpt
SDTHVTISGYPGEK S25140 180 46 -
VNQNITVTGYPGDK STSP_STAAU 193 48 -
VGTTVTISGYPGDK GSEP_BACLI 217 47 -
KGDKYSLLGYPYNY ETB_STAAU 177 48 -
VGLSSSVTGFPCDK MPR_BACSU 223 49 -
DGDKLELIGYPFDH ETA_STAAU 190 48 -
VNQNITVTGYPGDK S21758 193 48 -
VDDKIKVIGYPLPA SAU63529 148 46 -
AGERIKVIGYPHPY SAU60589 148 46 -
AGRKVTQAGYPEDH H64915 178 59 -

Motif 5 width=17
Element Seqn Id St Int Rpt
MQYDLSTTGGNSGSPVF STSP_STAAU 226 19 -
LFYDIDTTGGQSGSPIY S25140 217 23 -
MSHQCDTLPGDSGSPLM H64915 212 20 -
IVYSAHTESGNSGSPVL SAU60589 182 20 -
LNFDAYIEPGNSGSPVL SAU63529 182 20 -
LQYAMDTYGGQSGSPVF GSEP_BACLI 250 19 -
SQYFGYTEVGNSGSGIF ETB_STAAU 206 15 -
LTYTTDTYGCQSGSPVY MPR_BACSU 256 19 -
LRYYGFTVPGNSGSGIF ETA_STAAU 222 18 -
MQYDLSTTGGNSGSPVF S21758 226 19 -

Motif 6 width=13
Element Seqn Id St Int Rpt
NSNGELVGIHSSK ETA_STAAU 239 0 -
NAQFEVVGVHSNG S25140 234 0 -
DDGWQLIGVQSSA H64915 232 3 -
NSNNELVGIHFAS SAU60589 199 0 -
NSNNEVIGVVYGG SAU63529 199 0 -
NEKNEVIGIHWGG STSP_STAAU 243 0 -
CSGPCSLAVHTNG GSEP_BACLI 275 8 -
NLKGELIGIHSGK ETB_STAAU 223 0 -
DTGQTAIAIHTNG MPR_BACSU 277 4 -
NEKNEVIGIHWGG S21758 243 0 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
RHQITDTTNGHYAPVTYI Q04186 76 76 -
RHQITDTTNGHYAPVTYI STSP_STAAU 76 76 -
VTQVKDTNNFPYNGVVSF Q53782 40 40 -
VTKIQDTNIFPYTGVVAF Q53781 40 40 -
RTRVTNTTAYPYRAIVHI GSEP_BACLI 102 102 -
RQEVADTTEAPFASIGRI Q47809 55 55 -
YTHITDNARSPYNSVGTV ETB_STAAU 56 56 -
RTRISSTTSFPYRATVQL MPR_BACSU 101 101 -
KVDEKDRQKYPYNTIGNV ETA_STAAU 70 70 -
RVPVNDTTQSPWDAVGQL P76176 44 44 -

Motif 2 width=18
Element Seqn Id St Int Rpt
ASGVVVGKDTLLTNKHVV Q04186 104 10 -
ASGVVVGKDTLLTNKHVV STSP_STAAU 104 10 -
ATGFVIGKNTIITNKHVS Q53782 60 2 -
ATGFVVGKNTILTNKHVS Q53781 60 2 -
CTGWMIGPKTVATAGHCI GSEP_BACLI 126 6 -
GTGFVVGTNTIVTNNHVA Q47809 85 12 -
ATGVLIGKNTIVTNYHVA ETB_STAAU 81 7 -
CTGFLVNPNTVVTAGHCV MPR_BACSU 131 12 -
ATGVLIGKNTVLTNRHIA ETA_STAAU 95 7 -
CTATLIAPNLALTAGHCL P76176 69 7 -

Motif 3 width=13
Element Seqn Id St Int Rpt
KAFPSAINQDNYP Q04186 132 10 -
KAFPSAINQDNYP STSP_STAAU 132 10 -
HPNGDKGNGGIYK Q53782 89 11 -
HPNSDKGNGGIYS Q53781 89 11 -
VSPGRNGTSYPYG GSEP_BACLI 157 13 -
FYPGRDGSATPFG Q47809 121 18 -
RDAEKNEFPTPYG ETB_STAAU 116 17 -
AAPGRNGSSYPYG MPR_BACSU 161 12 -
NTDDNGNTETPYG ETA_STAAU 129 16 -
KGLWRYEIHDIEG P76176 106 19 -

Motif 4 width=14
Element Seqn Id St Int Rpt
VNQNITVTGYPGDK Q04186 193 48 -
VNQNITVTGYPGDK STSP_STAAU 193 48 -
VDDKIKVIGYPLPA Q53782 148 46 -
AGERIKVIGYPHPY Q53781 148 46 -
VGTTVTISGYPGDK GSEP_BACLI 217 47 -
SDTHVTISGYPGEK Q47809 180 46 -
KGDKYSLLGYPYNY ETB_STAAU 177 48 -
VGLSSSVTGFPCDK MPR_BACSU 223 49 -
DGDKLELIGYPFDH ETA_STAAU 190 48 -
AGRKVTQAGYPEDH P76176 178 59 -

Motif 5 width=17
Element Seqn Id St Int Rpt
MQYDLSTTGGNSGSPVF Q04186 226 19 -
MQYDLSTTGGNSGSPVF STSP_STAAU 226 19 -
LNFDAYIEPGNSGSPVL Q53782 182 20 -
IVYSAHTESGNSGSPVL Q53781 182 20 -
LQYAMDTYGGQSGSPVF GSEP_BACLI 250 19 -
LFYDIDTTGGQSGSPIY Q47809 217 23 -
SQYFGYTEVGNSGSGIF ETB_STAAU 206 15 -
LTYTTDTYGCQSGSPVY MPR_BACSU 256 19 -
LRYYGFTVPGNSGSGIF ETA_STAAU 222 18 -
MSHQCDTLPGDSGSPLM P76176 212 20 -

Motif 6 width=13
Element Seqn Id St Int Rpt
NEKNEVIGIHWGG Q04186 243 0 -
NEKNEVIGIHWGG STSP_STAAU 243 0 -
NSNNEVIGVVYGG Q53782 199 0 -
NSNNELVGIHFAS Q53781 199 0 -
CSGPCSLAVHTNG GSEP_BACLI 275 8 -
NAQFEVVGVHSNG Q47809 234 0 -
NLKGELIGIHSGK ETB_STAAU 223 0 -
DTGQTAIAIHTNG MPR_BACSU 277 4 -
NSNGELVGIHSSK ETA_STAAU 239 0 -
DDGWQLIGVQSSA P76176 232 3 -