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PR00827

Identifier
FELALLERGEN  [View Relations]  [View Alignment]  
Accession
PR00827
No. of Motifs
4
Creation Date
18-NOV-1997  (UPDATE 07-JUN-1999)
Title
Major allergen Fel Di signature
Database References

INTERPRO; IPR002412
Literature References
1. LEITERMANN, K. AND OHMAN, J.L.
Cat allergen 1: biochemical, antigenic, and allergenic properties. 
J.ALLERGY CLIN.IMMUNOL. 74 147-153 (1984). 
 
2. MORGENSTERN, J.P., GRIFFITH, I.J., BRAUER, A.W., ROGERS, B.L., BOND, J.F.,
CHAPMAN, M.D. AND KUO, M.C.
Amino acid sequence of Fel dI, the major allergen of the domestic cat:
protein sequence analysis and cDNA cloning. 
PROC.NATL.ACAD.SCI.U.S.A. 88 9690-9694(1991). 

Documentation
Allergen 1 from the domestic cat, Felis domesticus, is an important agent
in human allergic reactions [1]. The protein is expressed in saliva and 
sebaceous glands. The complete primary structure of Fel dI has been 
determined [2]. The allergen is composed of two polypeptide chains, which
have been shown to be encoded by different genes [2]. The protein exists as 
a heterotetramer of non-covalently linked disulphide-linked heterodimers of 
chains 1 and 2. Fel dI chain 1 shares sequence similarity with rabbit 
uteroglobin; chain 2 is a glycoprotein with N-linked oligosaccharides [2].
 
FELALLERGEN is a 4-element fingerprint that provides a signature for
feline Fel dI allergens. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from short conserved regions 
spanning virtually the full alignment length - motifs 1 and 4 reside at the
N- and C-termini respectively, and include the regions encoded by PROSITE 
patterns UTEROGLOBIN_1 (PS00403) and UTEROGLOBIN_2 (PS00404), each of which 
contains a Cys residue that is thought to be involved in interchain
disulphide bond formation. Two iterations on OWL29.6 were required to reach
convergence, at which point a true set comprising 6 sequences was identified. 
A single partial match was also found, a mouse salivary androgen-binding
protein that matches motifs 1,2 and 4.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
43333
30000
20000
1234
True Positives
FELA_FELCA    FELB_FELCA    O35176        
Sequence Titles
FELA_FELCA  MAJOR ALLERGEN I POLYPEPTIDE CHAIN 1 MAJOR FORM PRECURSOR (ALLERGEN FEL D 1-A) (FEL D I-A) (ALLERGEN CAT-1) (AG4) - FELIS SILVESTRIS CATUS (CAT). 
FELB_FELCA MAJOR ALLERGEN I POLYPEPTIDE CHAIN 1 MINOR FORM PRECURSOR (ALLERGEN FEL D 1-A) (FEL D I-A) (ALLERGEN CAT-1) (AG4) - FELIS SILVESTRIS CATUS (CAT).
O35176 LACRIMAL GLAND PROTEIN - MUS MUSCULUS (MOUSE).
Scan History
OWL29_6    2  25   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
DCEICPAVKRDVDLFL FELB_FELCA 17 17 -
NCEICPAVKRDVDLFL A56413 21 21 -
DCEICPAVKRDVDLFL FDFELDI11 21 21 -
NCEICPAVKRDVDLFL FELA_FELCA 21 21 -
DCEICPAVKRDVDLFL FDFELDI1 17 17 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LTGTPDEYVEQVAQYK FELA_FELCA 36 -1 -
LTGTPDEYVEQVAQYN FDFELDI11 36 -1 -
LTGTPDEYVEQVAQYK A56413 36 -1 -
LTGTPDEYVEQVAQYN FDFELDI1 32 -1 -
LTGTPDEYVEQVAQYK FELB_FELCA 32 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
NALPVVLENARILKNC FDFELDI1 47 -1 -
KALPVVLENARILKNC FELB_FELCA 47 -1 -
KALPVVLENARILKNC FELA_FELCA 51 -1 -
KALPVVLENARILKNC A56413 51 -1 -
NALPVVLENARILKNC FDFELDI11 51 -1 -

Motif 4 width=13
Element Seqn Id St Int Rpt
CVDAKMTEEDKEN FELA_FELCA 66 -1 -
CVDAKMTEEDKEN FDFELDI1 62 -1 -
CVDAKMTEEDKEN FDFELDI11 66 -1 -
CVDAKMTEEDKEN A56413 66 -1 -
CVDAKMTEEDKEN FELB_FELCA 62 -1 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
DCEICPAVKRDVDLFL FELB_FELCA 17 17 -
NCEICPAVKRDVDLFL FELA_FELCA 21 21 -
DCGICPAIKEDVHLFL O35176 22 22 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LTGTPDEYVEQVAQYK FELB_FELCA 32 -1 -
LTGTPDEYVEQVAQYK FELA_FELCA 36 -1 -
LFGTPEEYVNYVEKYK O35176 37 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
KALPVVLENARILKNC FELB_FELCA 47 -1 -
KALPVVLENARILKNC FELA_FELCA 51 -1 -
KDDPETLENTEKLKIC O35176 52 -1 -

Motif 4 width=13
Element Seqn Id St Int Rpt
CVDAKMTEEDKEN FELB_FELCA 62 -1 -
CVDAKMTEEDKEN FELA_FELCA 66 -1 -
CVDRTLTKENKEH O35176 67 -1 -