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PR00825

Identifier
DOLALLERGEN  [View Relations]  [View Alignment]  
Accession
PR00825
No. of Motifs
6
Creation Date
13-FEB-1998  (UPDATE 07-JUN-1999)
Title
Vespid venom allergen phospholipase A1 signature
Database References
PRINTS; PR00821 TAGLIPASE
PROSITE; PS00120 LIPASE_SER
BLOCKS; BL00120
INTERPRO; IPR002334
Literature References
1. HOFFMAN, D.R.
Allergens in hymenoptera venom. XXVI: the complete amino acid sequences
of two vespid venom phospholipases. 
INT.ARCH.ALLERGY IMMUNOL. 104 184-190 (1994). 
 
2.SOLDATOVA, L., KOCHOUMIAN, L. AND KING, T.P.
Sequence similarity of a hornet (D.maculata) venom allergen phospholipase
A1 with mammalian lipases.
FEBS LETT. 320 145-149 (1993). 

Documentation
The two principal allergenic proteins in vespid venoms are antigen 5s and
phospholipases [1]. The complete amino acid sequences of the venom
phospholipase A1 from the yellow jacket, Vespula maculifrons, and the more
acidic isoenzyme from the white faced hornet, Dolichovespula maculata, have
been determined [1,2]. The proteins, which contain ~300 amino acid residues,
show no sequence similarity to other known phospholipases, but do have some
similarity to mammalian lipases (~40% identity over 123 residues) [1,2].
Experimental evidence suggests that hornet phospholipase has weak lipase
activity [2].
 
DOLALLERGEN is a 6-element fingerprint that provides a signature for vespid
venom allergen phospholipase A1. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from short conserved regions 
spanning the C-terminal half of the alignment, focusing on those regions 
that characterise the venom phospholipases but distinguish them from related 
lipases. Two iterations on OWL29.6 were required to reach convergence, at
which point a true set comprising 4 sequences was identified. Several
partial matches were also found, all of which are closely related lipases
that match motifs 1 and 2.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6333333
5000000
4000000
3000000
2000000
123456
True Positives
PA12_DOLMA    PA1_VESMC     PA1_VESVU     
Sequence Titles
PA12_DOLMA  PHOSPHOLIPASE A1 2 (EC 3.1.1.32) (ALLERGEN DOL M 1.02) (DOL M I) - DOLICHOVESPULA MACULATA (WHITE-FACE HORNET) (BALD-FACED HORNET). 
PA1_VESMC PHOSPHOLIPASE A1 (EC 3.1.1.32) (ALLERGEN VES M 1) (VES M I) - VESPULA MACULIFRONS (EASTERN YELLOW JACKET) (WASP).
PA1_VESVU PHOSPHOLIPASE A1 PRECURSOR (EC 3.1.1.32) (ALLERGEN VES V 1) (VES V I) - VESPULA VULGARIS (YELLOW JACKET) (WASP).
Scan History
OWL29_6    2  100  NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
DCSERICETDAHYVQILH PA11_DOLMA 192 192 -
DCPERICETDAHYVQILH PA12_DOLMA 178 178 -

Motif 2 width=21
Element Seqn Id St Int Rpt
LGTERTLGTVDFYINNGSNQP PA11_DOLMA 214 4 -
LGTERTLGTVDFYINDGSNQP PA12_DOLMA 200 4 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GCRYIIGETCSHTRAVKY PA11_DOLMA 235 0 -
GCTYIIGETCSHTRAVKY PA12_DOLMA 221 0 -

Motif 4 width=18
Element Seqn Id St Int Rpt
ECIRRECCLIGVPQSKNP PA11_DOLMA 255 2 -
ECIRRECCLIGVPQSKNP PA12_DOLMA 241 2 -

Motif 5 width=18
Element Seqn Id St Int Rpt
KCTRNECVCVGLNAKKYP PA11_DOLMA 277 4 -
KCTRNECVCVGLNAKEYP PA12_DOLMA 263 4 -

Motif 6 width=21
Element Seqn Id St Int Rpt
KRGSFYVPVEAEAPYCNNNGK PA11_DOLMA 295 0 -
KKGSFYVPVEAKAPFCNNNGK PA12_DOLMA 281 0 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
HCSERLCETDAEYVQIIH PA1_VESVU 211 211 -
HCSERLCETDAEYVQIIH PA1_VESMC 175 175 -
DCPERICETDAHYVQILH PA12_DOLMA 178 178 -

Motif 2 width=21
Element Seqn Id St Int Rpt
LGTEKTLGTVDFYMNNGKNQP PA1_VESVU 233 4 -
LGTEKILGTVDFYMNNGKNNP PA1_VESMC 197 4 -
LGTERTLGTVDFYINDGSNQP PA12_DOLMA 200 4 -

Motif 3 width=18
Element Seqn Id St Int Rpt
GCGRFFSEVCSHSRAVIY PA1_VESVU 254 0 -
GCGRFFSEVCSHTRAVIY PA1_VESMC 218 0 -
GCTYIIGETCSHTRAVKY PA12_DOLMA 221 0 -

Motif 4 width=18
Element Seqn Id St Int Rpt
ECIKHECCLIGIPKSKSS PA1_VESVU 274 2 -
ECIKHECCLIGIPRSKSS PA1_VESMC 238 2 -
ECIRRECCLIGVPQSKNP PA12_DOLMA 241 2 -

Motif 5 width=18
Element Seqn Id St Int Rpt
SCTKQECVCVGLNAKKYP PA1_VESVU 296 4 -
RCTKQECVCVGLNAKKYP PA1_VESMC 260 4 -
KCTRNECVCVGLNAKEYP PA12_DOLMA 263 4 -

Motif 6 width=21
Element Seqn Id St Int Rpt
SRGSFYVPVESTAPFCNNKGK PA1_VESVU 314 0 -
SRGSFYVPVESTAPFCNNKGK PA1_VESMC 278 0 -
KKGSFYVPVEAKAPFCNNNGK PA12_DOLMA 281 0 -