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PR00822

Identifier
LIPOLIPASE  [View Relations]  [View Alignment]  
Accession
PR00822
No. of Motifs
8
Creation Date
18-FEB-1998  (UPDATE 07-JUN-1999)
Title
Lipoprotein lipase signature
Database References
PRINTS; PR00821 TAGLIPASE
PROSITE; PS00120 LIPASE_SER
BLOCKS; BL00120
INTERPRO; IPR002330
Literature References
1. WION, K.L., KIRCHGESSNER, T.G., LUSIS, A.J., SCHOTZ, M.C. AND LAWN, R.M.
Human lipoprotein lipase complementary DNA sequence.
SCIENCE 235 1638-1641 (1987). 
 
2. REINA, M., BRUNZELL, J.D. AND DEEB, S.S.
Molecular basis of familial chylomicronemia: mutations in the lipoprotein
lipase and apolipoprotein C-II genes.
J.LIPID RES. 33 1823-1832 (1992). 
 
3. VAN TILBEURGH, H., ROUSSEL, A., LALOUEL, J.M. AND CAMBILLAU, C.
Lipoprotein lipase. molecular model based on the pancreatic lipase X-ray
structure: consequences for heparin binding and catalysis. 
J.BIOL.CHEM. 269 4626-4633 (1994). 

Documentation
Lipoprotein lipase (LPL) is a key enzyme of lipid metabolism that hydrolyses
triglycerides, providing free fatty acids for cells and affecting the 
maturation of circulating lipoproteins [1]. The enzyme is thought to play
a role in the development of obesity and atherosclerosis [1]. Human LPL
contains 448 amino acids; sequence comparison indicates that human LPL,
hepatic lipase, and pancreatic lipase are members of a gene family [1]. 
 
Defects in LPL are a cause of familial chylomicronemia syndrome (or type I
hyperlipoproteinemia) and also of a form of deficiency characterised by
hypertriglyceridemia. Familial chylomicronemia is a recessive disorder
usually manifesting in childhood. On a normal diet, patients often present
with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive
xanthomata, and massive hypertriglyceridemia, sometimes complicated with
acute pancreatitis.
 
LPL and pancreatic lipase share ~30% sequence identity, suggesting a similar
tertiary fold [3]. Molecular models of LPL have been constructed, based on 
X-ray crystal structures of pancreatic lipase [2]. These models allowed the
authors to propose hypotheses on the structural determinants of LPL that are
responsible for heparin binding, dimer formation, and phospholipase activity. 
 
LIPOLIPASE is an 8-element fingerprint that provides a signature for
lipoprotein lipases. The fingerprint was derived from an initial alignment
of 7 sequences: the motifs were drawn from conserved sections spanning the
full alignment length, focusing on those regions that characterise the LPLs
but distinguish them from the rest of the lipase family. Two iterations on
OWL30.0 were required to reach convergence, at which point a true set
comprising 10 sequences was identified. A single partial match was also
found, a partial lipase sequence from the zebrafish that matches motifs 4,
5, 6 and 8. 
 
An update on SPTR37_9f identified a true set of 10 sequences.
Summary Information
10 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
81010101010101010
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
LIPL_CAVPO    LIPL_CHICK    LIPL_FELCA    LIPL_HUMAN    
LIPL_MOUSE LIPL_PAPAN LIPL_PIG LIPL_RAT
LIPL_SHEEP O46647
Sequence Titles
LIPL_CAVPO  LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - CAVIA PORCELLUS (GUINEA PIG). 
LIPL_CHICK LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - GALLUS GALLUS (CHICKEN).
LIPL_FELCA LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - FELIS SILVESTRIS CATUS (CAT).
LIPL_HUMAN LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - HOMO SAPIENS (HUMAN).
LIPL_MOUSE LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - MUS MUSCULUS (MOUSE).
LIPL_PAPAN LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - PAPIO ANUBIS (OLIVE BABOON).
LIPL_PIG LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - SUS SCROFA (PIG).
LIPL_RAT LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - RATTUS NORVEGICUS (RAT).
LIPL_SHEEP LIPOPROTEIN LIPASE PRECURSOR (EC 3.1.1.34) (LPL) - OVIS ARIES (SHEEP).
O46647 LIPOPROTEIN LIPASE PRECURSOR - MUSTELA VISON (AMERICAN MINK).
Scan History
OWL30_0    2  100  NSINGLE    
SPTR37_9f 2 11 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
ALRTPEDTAEDTCHLIPG LIPL_BOVIN 32 32 -
ALRTPEDTAEDTCHLIPG LIPL_PAPAN 42 42 -
ALRTPEDTAEDTCHLIPG LIPL_HUMAN 42 42 -
ALRTPEDIAEDTCHLIPG LIPL_FELCA 45 45 -
ALRTPEDTAEDTCHLIPG LIPL_RAT 42 42 -
ALRTPEDTAEDTCHLIPG LIPL_MOUSE 42 42 -
SLRTPAEPDEDVCYLVPG LIPL_CHICK 42 42 -

Motif 2 width=25
Element Seqn Id St Int Rpt
TVTGMYESWVPKLVAALYKREPDSN LIPL_BOVIN 73 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_HUMAN 83 23 -
TVTGMYESWVPKLVAAPYKREPDSN LIPL_FELCA 86 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_RAT 83 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_MOUSE 83 23 -
TVTGMYESWVPKLVDALYKREPDSN LIPL_CHICK 83 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_PAPAN 83 23 -

Motif 3 width=17
Element Seqn Id St Int Rpt
LSRAQQHYPVSAGYTKL LIPL_PAPAN 114 6 -
LSRAQQHYPVSAGYTKL LIPL_FELCA 117 6 -
LSRAQQHYPVSAGYTKL LIPL_BOVIN 104 6 -
LVRAQQHYPVSAAYTKL LIPL_CHICK 114 6 -
LYRAQQHYPVSAGYTKL LIPL_MOUSE 114 6 -
LYRAQQHYPVSAGYTKL LIPL_RAT 114 6 -
LSRAQEHYPVSAGYTKL LIPL_HUMAN 114 6 -

Motif 4 width=13
Element Seqn Id St Int Rpt
GIAGSLTNKKVNR LIPL_FELCA 169 35 -
GIAGSLTNKKVNR LIPL_BOVIN 156 35 -
GIAGSLTNKKVNR LIPL_PAPAN 166 35 -
GIAGSLTNKKVNR LIPL_HUMAN 166 35 -
GVAGSLTNKKVNR LIPL_RAT 166 35 -
GVAGSLTNKKVNR LIPL_MOUSE 166 35 -
GIAGSLTKKKVNR LIPL_CHICK 166 35 -

Motif 5 width=17
Element Seqn Id St Int Rpt
RGSPGRSIGIQKPVGHV LIPL_RAT 214 35 -
RGSPGRSIGIQKPVGHV LIPL_BOVIN 204 35 -
RGSPDRSIGIQKPVGHI LIPL_CHICK 214 35 -
RGSPGRSIGIQKPVGHV LIPL_MOUSE 214 35 -
RGSPGRSIGIQKPVGHV LIPL_PAPAN 214 35 -
RGSPGRSIGIQKPVGHV LIPL_HUMAN 214 35 -
RGSPGRSIGIQKPVGHV LIPL_FELCA 217 35 -

Motif 6 width=16
Element Seqn Id St Int Rpt
IHFSGTENGKQHNQAF LIPL_MOUSE 347 116 -
IHFSGTESETHTNQAF LIPL_PAPAN 347 116 -
IHFSGTESETHTNQAF LIPL_HUMAN 347 116 -
IHFSGTESDTQTNQVF LIPL_FELCA 350 116 -
IHFSGTENDKQNNQAF LIPL_RAT 347 116 -
IHFFGKTNVTKVDQPF LIPL_CHICK 347 116 -
IHFSGTESNTYTNQAF LIPL_BOVIN 337 116 -

Motif 7 width=18
Element Seqn Id St Int Rpt
TFFSWSDWWTPFAFTIQR LIPL_CHICK 413 50 -
SYFSWSDWWSSPGFAIQK LIPL_PAPAN 413 50 -
SYFSWSDWWSSPGFAIQK LIPL_HUMAN 413 50 -
SYFSWSDWWSSPGFTIEK LIPL_FELCA 416 50 -
SYFRWSDWWSSPSFVIEK LIPL_RAT 413 50 -
SYFSWSNWWSSPGFDIGK LIPL_BOVIN 403 50 -
SYFSWPDWWSSPSFVIER LIPL_MOUSE 413 50 -

Motif 8 width=24
Element Seqn Id St Int Rpt
REKVSHLQKGKASVVFVKCHDKSL LIPL_FELCA 450 16 -
REKVSHLQKGKAPAVFVKCHDKSL LIPL_HUMAN 447 16 -
RDGSSRLGKGEEAAIFVKCLEQPV LIPL_CHICK 447 16 -
REKVSHLQKGKDSAVFVKCHDKSL LIPL_MOUSE 447 16 -
REKVSHLQKGKAPAVFVKCHDKSL LIPL_PAPAN 447 16 -
REKMSYLQKGKSPVIFVKCHDKSL LIPL_BOVIN 437 16 -
REKVSHLQKGKDAAVFVKCHDKSL LIPL_RAT 447 16 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
ALRTPEDTAEDTCHLIPG O46647 42 42 -
ALRTPEDTAEDTCHLIPG LIPL_PAPAN 42 42 -
ALRTPEDTAEDTCHLIPG LIPL_HUMAN 42 42 -
ALRTPEDIAEDTCHLIPG LIPL_FELCA 45 45 -
ALRTPEDTAEDTCHLIPG LIPL_SHEEP 45 45 -
ALRTPEDTAEDTCHLIPG LIPL_RAT 42 42 -
ALRTPEDTVEDTCHLIPG LIPL_PIG 45 45 -
ALRTPEDTAEDTCHLIPG LIPL_MOUSE 42 42 -
ARRTPENTVEDTCHLIPG LIPL_CAVPO 32 32 -
SLRTPAEPDEDVCYLVPG LIPL_CHICK 42 42 -

Motif 2 width=25
Element Seqn Id St Int Rpt
TVTGMYESWVPKLVAALYKREPDSN O46647 83 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_PAPAN 83 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_HUMAN 83 23 -
TVTGMYESWVPKLVAAPYKREPDSN LIPL_FELCA 86 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_SHEEP 86 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_RAT 83 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_PIG 86 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_MOUSE 83 23 -
TVTGMYESWVPKLVAALYKREPDSN LIPL_CAVPO 73 23 -
TVTGMYESWVPKLVDALYKREPDSN LIPL_CHICK 83 23 -

Motif 3 width=17
Element Seqn Id St Int Rpt
LSRAQQHYPVSAGYTKL O46647 114 6 -
LSRAQQHYPVSAGYTKL LIPL_PAPAN 114 6 -
LSRAQEHYPVSAGYTKL LIPL_HUMAN 114 6 -
LSRAQQHYPVSAGYTKL LIPL_FELCA 117 6 -
LSRAQQHYPVSAGYTKL LIPL_SHEEP 117 6 -
LYRAQQHYPVSAGYTKL LIPL_RAT 114 6 -
LSRAQQHYPISAGYTKL LIPL_PIG 117 6 -
LYRAQQHYPVSAGYTKL LIPL_MOUSE 114 6 -
LRRAQHHYPESADYTKL LIPL_CAVPO 104 6 -
LVRAQQHYPVSAAYTKL LIPL_CHICK 114 6 -

Motif 4 width=13
Element Seqn Id St Int Rpt
GIAGSLTNKKVNR O46647 166 35 -
GIAGSLTNKKVNR LIPL_PAPAN 166 35 -
GIAGSLTNKKVNR LIPL_HUMAN 166 35 -
GIAGSLTNKKVNR LIPL_FELCA 169 35 -
GIAGSLTNKKVNR LIPL_SHEEP 169 35 -
GVAGSLTNKKVNR LIPL_RAT 166 35 -
GIAGSLTKKKVNR LIPL_PIG 169 35 -
GVAGSLTNKKVNR LIPL_MOUSE 166 35 -
GVAGSRTNTKVSR LIPL_CAVPO 156 35 -
GIAGSLTKKKVNR LIPL_CHICK 166 35 -

Motif 5 width=17
Element Seqn Id St Int Rpt
RGSPGRSIGIQKPVGHV O46647 214 35 -
RGSPGRSIGIQKPVGHV LIPL_PAPAN 214 35 -
RGSPGRSIGIQKPVGHV LIPL_HUMAN 214 35 -
RGSPGRSIGIQKPVGHV LIPL_FELCA 217 35 -
RGSPGRSIGIQKPVGHV LIPL_SHEEP 217 35 -
RGSPGRSIGIQKPVGHV LIPL_RAT 214 35 -
RGSPGRSIGIQKPVGHV LIPL_PIG 217 35 -
RGSPGRSIGIQKPVGHV LIPL_MOUSE 214 35 -
RGSPGRSIGIQKPVGHV LIPL_CAVPO 204 35 -
RGSPDRSIGIQKPVGHI LIPL_CHICK 214 35 -

Motif 6 width=16
Element Seqn Id St Int Rpt
IHFSGTESDTQTNQAF O46647 347 116 -
IHFSGTESETHTNQAF LIPL_PAPAN 347 116 -
IHFSGTESETHTNQAF LIPL_HUMAN 347 116 -
IHFSGTESDTQTNQVF LIPL_FELCA 350 116 -
IHFSGTESNTYTNQAF LIPL_SHEEP 350 116 -
IHFSGTENDKQNNQAF LIPL_RAT 347 116 -
MRFSGTESDTHTNQAF LIPL_PIG 350 116 -
IHFSGTENGKQHNQAF LIPL_MOUSE 347 116 -
IYFSGTETTTYTNQAF LIPL_CAVPO 337 116 -
IHFFGKTNVTKVDQPF LIPL_CHICK 347 116 -

Motif 7 width=18
Element Seqn Id St Int Rpt
SYFSWSDWWSSPGFAIEK O46647 413 50 -
SYFSWSDWWSSPGFAIQK LIPL_PAPAN 413 50 -
SYFSWSDWWSSPGFAIQK LIPL_HUMAN 413 50 -
SYFSWSDWWSSPGFTIEK LIPL_FELCA 416 50 -
SYFSWSNWWSSPGFDIGK LIPL_SHEEP 416 50 -
SYFRWSDWWSSPSFVIEK LIPL_RAT 413 50 -
SYFSWSNWWSSPGFAIEK LIPL_PIG 416 50 -
SYFSWPDWWSSPSFVIER LIPL_MOUSE 413 50 -
SYFSWSSWWGRPTFTIEK LIPL_CAVPO 403 50 -
TFFSWSDWWTPFAFTIQR LIPL_CHICK 413 50 -

Motif 8 width=24
Element Seqn Id St Int Rpt
REKVSHLQKGKASVVFVKCHDKSL O46647 447 16 -
REKVSHLQKGKAPAVFVKCHDKSL LIPL_PAPAN 447 16 -
REKVSHLQKGKAPAVFVKCHDKSL LIPL_HUMAN 447 16 -
REKVSHLQKGKASVVFVKCHDKSL LIPL_FELCA 450 16 -
REKMSYLQKGKSPVIFVKCHDKSL LIPL_SHEEP 450 16 -
REKVSHLQKGKDAAVFVKCHDKSL LIPL_RAT 447 16 -
REKKSHLQKGKSSVVFVKCHDKSL LIPL_PIG 450 16 -
REKVSHLQKGKDSAVFVKCHDKSL LIPL_MOUSE 447 16 -
REKVSKLQKGKEAPVFVKCHDKSL LIPL_CAVPO 437 16 -
RDGSSRLGKGEEAAIFVKCLEQPV LIPL_CHICK 447 16 -