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PR00813

Identifier
BCTERIALGSPG  [View Relations]  [View Alignment]  
Accession
PR00813
No. of Motifs
2
Creation Date
19-MAR-1998  (UPDATE 10-JUN-1999)
Title
Bacterial general secretion pathway protein G signature
Database References

PROSITE; PS00409 PROKAR_NTER_METHYL
BLOCKS; BL00409
PFAM; PF00114 pilin
INTERPRO; IPR000983
Literature References
1. SALMOND, G.P.C. AND REEVES, P.J.
Membrane traffic wardens and protein secretion in Gram-negative bacteria.
TRENDS BIOCHEM.SCI. 18 7-12 (1993).
 
2. WANDERSMAN, C.
Secretion across the bacterial outer membrane.
TRENDS GENET. 8(9) 317-321 (1992).
 
3. LORY, S.
Determinants of extracellular protein secretion in Gram-negative bacteria.
J.BACTERIOL. 174(11) 3423-3428 (1992) 
 
4. HOWARD, S.P., CRITCH, J. AND BEDI, A.
Isolation and analysis of the eight exe genes and their involvement in 
extracellular protein secretion and outer membrane assembly in Aeromonas
hydrophila.
J.BACTERIOL. 175(20) 6695-6703 (1993).
 
5. STOJILJKOVI, I., SCHONHERR, R. AND KUSTERS, J.G.
Identification of the HopG gene, a component of Escherichia coli K-12 type
II export system, and its conservation among different pathogenic Escherichia
coli and Shigella isolates.
J.BACTERIOL. 177(7) 1892-1895 (1995).

Documentation
The general (type II) secretion pathway (GSP) within Gram-negative bacteria
is a signal sequence-dependent process repsonsible for protein export [1-3].
The process has two stages: exoproteins are first translocated across the
inner membrane by the general signal-dependent export pathway (GEP), and 
then across the outer membrane by a species-specific accessory mechanism. 
 
Several proteins are involved in the GSP; one of these is termed general  
secretion pathway protein G (GSPG). This protein shares several sequence
similarities with bacterial fimbrial protein, or pilin, the major structural
protein of pili [4,5]. Pili are polar flexible filamentous adhesions ~2500nm  
in length, and diameter ~5.4nm. Fimbrial and GSPG proteins share the 
following characteristics: a methylated, hydrophobic N-terminal residue; a 
hydrophobic leader peptide of 5-10 residues, terminating with glycine; 
glutamate as the fifth residue of the mature sequence; and a highly
hydrophobic N-terminus.
 
BCTERIALGSPG is a 2-element fingerprint that provides a signature for 
general secretion pathway protein G. The fingerprint was derived from an
initial alignment of 8 sequences: the motifs were drawn from conserved
regions in the N-terminal portion of the alignment - motif 1 includes the
hydrophobic region encoded by PROSITE pattern PROKAR_NTER_METHYL (PS00409).
Two iterations on OWL30.0 were required to reach convergence, at which
point a true set comprising 10 sequences was identified. 
 
An update on SPTR37_9f identified a true set of 12 sequences.
Summary Information
12 codes involving  2 elements
Composite Feature Index
21212
12
True Positives
GSPG_AERHY    GSPG_ECOLI    GSPG_ERWCA    GSPG_ERWCH    
GSPG_KLEPN GSPG_PSEAE GSPG_VIBCH GSPG_XANCP
O32569 O66734 O69084 Q52294
Sequence Titles
GSPG_AERHY  GENERAL SECRETION PATHWAY PROTEIN G PRECURSOR - AEROMONAS HYDROPHILA. 
GSPG_ECOLI PUTATIVE GENERAL SECRETION PATHWAY PROTEIN G PRECURSOR (PROTEIN TRANSPORT PROTEIN HOFG) - ESCHERICHIA COLI.
GSPG_ERWCA GENERAL SECRETION PATHWAY PROTEIN G PRECURSOR (PECTIC ENZYMES SECRETION PROTEIN OUTG) - ERWINIA CAROTOVORA.
GSPG_ERWCH GENERAL SECRETION PATHWAY PROTEIN G PRECURSOR (PECTIC ENZYMES SECRETION PROTEIN OUTG) - ERWINIA CHRYSANTHEMI.
GSPG_KLEPN GENERAL SECRETION PATHWAY PROTEIN G PRECURSOR (PULLULANASE SECRETION PROTEIN PULG) - KLEBSIELLA PNEUMONIAE.
GSPG_PSEAE GENERAL SECRETION PATHWAY PROTEIN G PRECURSOR (PILD-DEPENDENT PROTEIN PDDA) - PSEUDOMONAS AERUGINOSA.
GSPG_VIBCH GENERAL SECRETION PATHWAY PROTEIN G PRECURSOR (CHOLERA TOXIN SECRETION PROTEIN EPSG) - VIBRIO CHOLERAE.
GSPG_XANCP GENERAL SECRETION PATHWAY PROTEIN G PRECURSOR - XANTHOMONAS CAMPESTRIS (PV. CAMPESTRIS).
O32569 ETPG PROTEIN - ESCHERICHIA COLI.
O66734 GENERAL SECRETION PATHWAY PROTEIN G - AQUIFEX AEOLICUS.
O69084 SECRETION PROTEIN XCPT - PSEUDOMONAS PUTIDA GB-1.
Q52294 XCPT PROTEIN - PSEUDOMONAS PUTIDA.
Scan History
OWL30_0    2  300  NSINGLE    
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=26
Element Seqn Id St Int Rpt
GFTLLEIMVVIVIIGVLASLVVPNLM GSPG_ECOLI 9 9 -
GFTLLEVMVVIVILGILASLVVPNLM GSPG_AERHY 8 8 -
GFTLLEIMVVIVILGVLASLVVPNLM GSPG_ERWCA 22 22 -
GFTLLEIMVVIVILGVLASLVVPNLM GSPG_ERWCH 7 7 -
GFTLLEIMVVIVILGVLASLVVPNLM GSPG_KLEPN 6 6 -
GFTLLEVMVVVVILGILASFVVPNLL GSPG_VIBCH 9 9 -
GFTLIEIMVVVVILGILAALVVPQVM GSPG_PSEAE 8 8 -
GMSLLEIIIVIVLIGAVLTLVGSRVL GSPG_XANCP 17 17 -

Motif 2 width=19
Element Seqn Id St Int Rpt
AVSDIVALENALDMYKLDN GSPG_ECOLI 45 10 -
AVSDIVALENALDMYKLDN GSPG_AERHY 44 10 -
AVSDIVSLESALDMYKLDN GSPG_ERWCA 58 10 -
AVSDIVALESQLDMYKLDN GSPG_ERWCH 43 10 -
VVSDLVALEGALDMYKLDN GSPG_KLEPN 42 10 -
AVTDIVALENALDMYKLDN GSPG_VIBCH 45 10 -
AKGDIKAIAAALDMYKLDN GSPG_PSEAE 44 10 -
AKSQIQTLAGKIENFQLDT GSPG_XANCP 53 10 -
Final Motifs
Motif 1  width=26
Element Seqn Id St Int Rpt
GFTLLEIMVVIVIIGVLASLVVPNLM GSPG_ECOLI 9 9 -
GFTLLEVMVVIVILGILASLVVPNLM GSPG_AERHY 8 8 -
GFTLLEIMVVIVILGVLASLVVPNLM GSPG_ERWCA 22 22 -
GFTLLEIMVVIVILGVLASLVVPNLM GSPG_ERWCH 7 7 -
GFTLLEIMVVIVILGVLASLVVPNLM GSPG_KLEPN 6 6 -
GFTLLEVMVVVVILGILASFVVPNLL GSPG_VIBCH 9 9 -
GFTLLEIMVVIVILGVLASLVVPNLM O32569 8 8 -
GFTLIEIMVVVVILGILAALVVPQVM GSPG_PSEAE 8 8 -
GFTLMEIMVVIFIIGLLIAVVAPSVL Q52294 10 10 -
GMSLLEIIIVIVLIGAVLTLVGSRVL GSPG_XANCP 17 17 -
GFTLIEVLVVLIILGLLAALIVPKLT O66734 4 4 -
GFTLLELLVVLVVLGLLAGIVAPKYF O69084 10 10 -

Motif 2 width=19
Element Seqn Id St Int Rpt
AVSDIVALENALDMYKLDN GSPG_ECOLI 45 10 -
AVSDIVALENALDMYKLDN GSPG_AERHY 44 10 -
AVSDIVSLESALDMYKLDN GSPG_ERWCA 58 10 -
AVSDIVALESQLDMYKLDN GSPG_ERWCH 43 10 -
VVSDLVALEGALDMYKLDN GSPG_KLEPN 42 10 -
AVTDIVALENALDMYKLDN GSPG_VIBCH 45 10 -
VMSDLVALESTLDMYRLDN O32569 44 10 -
AKGDIKAIAAALDMYKLDN GSPG_PSEAE 44 10 -
VMADLATLEQALDMYRLDN Q52294 46 10 -
AKSQIQTLAGKIENFQLDT GSPG_XANCP 53 10 -
TKLQLKAVKNALEQFKLDN O66734 40 10 -
ARAQIEGLSKALDLYRLEV O69084 46 10 -