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PR00806

Identifier
VINCULIN  [View Relations]  [View Alignment]  
Accession
PR00806
No. of Motifs
7
Creation Date
07-MAR-1998  (UPDATE 03-JUL-1999)
Title
Vinculin signature
Database References

PROSITE; PS00663 VINCULIN_1; PS00664 VINCULIN_2
BLOCKS; BL00663
INTERPRO; IPR000633
Literature References
1. OTTO, J.J.
vinculin.
CELL MOTIL.CYTOSKELETON 16 1-6 (1990).
 
2. JONES, P., JACKSON, P., PRICE, G.J., PATEL, B., OHANION, V., LEAR, A.L.
AND CRITCHLEY, D.R.
Identification of a talin binding site in the cytoskeletal protein vinculin. 
J.CELL BIOL. 109 2917-2927 (1989).
 
3. HERRENKNECHT, K., OZAWA, M., ECKERSKORN, C., LOTTSPEICH, F., LENTER, M.
AND KEMLER, R.
The uvomorulin-anchorage protein alpha-catenin is a vinculin homolog.
PROC.NATL.ACAD.SCI.U.S.A. 88 9156-9160 (1991).
 
4. WELLER, P.A., OGRYZKO, E.P., CORBEN, E.B., ZHIDKOVA, N.I., PATEL, B.,
PRICE, G.J., SPURR, N.K., KOTELIANSKY, V.E. AND CRITCHLEY, D.R.
Complete sequence of human vinculin and assignment of the gene to
chromosome 10. 
PROC.NATL.ACAD.SCI.U.S.A. 87 5667-5671 (1990). 

Documentation
Vinculin is a eukaryotic protein that appears to be involved in the
attachment of actin-based microfilaments to the plasma membrane [1].  
It also interacts with other structural proteins, such as talin [2] and
alpha-actinin. The protein is located on the cytoplasmic side of focal
contacts or adhesion plaques.
 
Vinculin is a large protein (~1000 residues) that contains an acidic
N-terminal domain, separated from a smaller, basic C-terminal domain by a
50-residue proline-rich region [3]. The central part of the N-terminal
domain consists of a variable number of repeats of a 110-residue domain,
one of which is lacking in nematode vinculin [4]. Alpha-catenins are 
evolutionarily related to vinculin, but differ in terms of their structure
in lacking the repeated domain and proline-rich segment.
 
VINCULIN is a 7-element fingerprint that provides a signature for
vinculins. The fingerprint was derived from an initial alignment of 6
sequences: the motifs were drawn from conserved regions spanning the
C-terminal half of the alignment - motifs 1 and 2 lie in the proline-rich
region; and motifs 3-7 lie in the C-terminal domain. Two iterations on
OWL30.0 were required to reach convergence, at which point a true set
comprising 8 sequences was identified. Two partial matches were also found,
S87175S3 and VINC_XENLA, both of which are vinculin fragments.
 
An update on SPTR37_9f identified a true set of 7 sequences, and 9
partial matches.
Summary Information
   7 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
6 codes involving 2 elements
Composite Feature Index
77777777
60000000
50000000
40000000
30000000
20060006
1234567
True Positives
O46037        Q17162        Q24584        VINC_CAEEL    
VINC_CHICK VINC_HUMAN VINC_MOUSE
True Positive Partials
Codes involving 2 elements
CTN1_HUMAN CTN1_MOUSE CTN2_CHICK CTN2_HUMAN
CTN2_MOUSE Q12795
Sequence Titles
O46037      COSMID 103B4 - DROSOPHILA MELANOGASTER (FRUIT FLY). 
Q17162 VINCULIN - BRUGIA MALAYI.
Q24584 VINCULIN - DROSOPHILA MELANOGASTER (FRUIT FLY).
VINC_CAEEL VINCULIN (P107B) - CAENORHABDITIS ELEGANS.
VINC_CHICK VINCULIN - GALLUS GALLUS (CHICKEN).
VINC_HUMAN VINCULIN - HOMO SAPIENS (HUMAN).
VINC_MOUSE VINCULIN - MUS MUSCULUS (MOUSE).

CTN1_HUMAN ALPHA-1 CATENIN (CADHERIN-ASSOCIATED PROTEIN) (ALPHA E-CATENIN) - HOMO SAPIENS (HUMAN).
CTN1_MOUSE ALPHA-1 CATENIN (102 KD CADHERIN-ASSOCIATED PROTEIN) (CAP102) (ALPHA E-CATENIN) - MUS MUSCULUS (MOUSE).
CTN2_CHICK ALPHA-2 CATENIN (ALPHA N-CATENIN) (NEURAL ALPHA-CATENIN) - GALLUS GALLUS (CHICKEN).
CTN2_HUMAN ALPHA-2 CATENIN (ALPHA-CATENIN RELATED PROTEIN) (ALPHA N-CATENIN) - HOMO SAPIENS (HUMAN).
CTN2_MOUSE ALPHA-2 CATENIN (ALPHA-CATENIN RELATED PROTEIN) (ALPHA N-CATENIN) - MUS MUSCULUS (MOUSE).
Q12795 ALPHA2(E)-CATENIN - HOMO SAPIENS (HUMAN).
Scan History
OWL30_0    2  50   NSINGLE    
SPTR37_9f 2 98 NSINGLE
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
DIPAPPRPPPP VINC_CAEEL 784 784 -
DELAPPKPPLP VINC_HUMAN 855 855 -
DELAPPKPPLP VINC_CHICK 855 855 -
DELAPPKPPLP MUSVINCULX 856 856 -
AERAPPRPPLP DMC103B413 715 715 -
DELAPPKPPLP VINC_PIG 60 60 -

Motif 2 width=14
Element Seqn Id St Int Rpt
APVRPPPPETDDED DMC103B413 730 4 -
PPPRPPPPEEKDEE VINC_PIG 75 4 -
PPPRPPPPEEKDEE MUSVINCULX 871 4 -
PPPRPPPPEEKDEE VINC_CHICK 870 4 -
PPPRPPPPEEKDEE VINC_HUMAN 870 4 -
PPPRPPPPPEYDEE VINC_CAEEL 799 4 -

Motif 3 width=18
Element Seqn Id St Int Rpt
NEIIAAAKRMAILMARLS DMC103B413 776 32 -
NDIVAAAKRMAILMARLS VINC_CAEEL 851 38 -
NDIIAAAKRMALLMAEMS VINC_HUMAN 916 32 -
NDIIAAAKRMALLMAEMS VINC_CHICK 916 32 -
NDIIAAAKRMALLMAEMS VINC_PIG 187 98 -
NDIIAAAKRMALLMAEMS MUSVINCULX 917 32 -

Motif 4 width=16
Element Seqn Id St Int Rpt
KKIAEASEDVTRLAKE DMC103B413 812 18 -
KDIAKASDEVTRLAKE VINC_CHICK 951 17 -
KDIAKASDEVTRLAKE VINC_HUMAN 951 17 -
KAIADSSEEVTRLAVQ VINC_CAEEL 886 17 -
KDIAKASDEVTRLAKE VINC_PIG 222 17 -
KDIAKASDEVTRLAKE MUSVINCULX 952 17 -

Motif 5 width=16
Element Seqn Id St Int Rpt
EVAKQCTDKRIRTNLL VINC_HUMAN 966 -1 -
QLARLCTDIKMRTALL VINC_CAEEL 901 -1 -
EVAKQCTDKRIRTNLL VINC_PIG 237 -1 -
EVAKQCTDKRIRTNLL VINC_CHICK 966 -1 -
ELARQCTDRRIRTNLL DMC103B413 827 -1 -
EVAKQCTDKRIRTNLL MUSVINCULX 967 -1 -

Motif 6 width=22
Element Seqn Id St Int Rpt
ERIPTISTQLKILSTVKATMLG MUSVINCULX 986 3 -
ERIPTISTQLKILSTVKATMLG VINC_PIG 256 3 -
ERIPTISTQLKILSTVKATMLG VINC_HUMAN 985 3 -
ERIPTIATQLKVLSTVKATMLG VINC_CAEEL 920 3 -
ERIPTISTQLKILSTVKATMLG VINC_CHICK 985 3 -
ERIPTIGTQLKILSTVKATMLG DMC103B413 846 3 -

Motif 7 width=20
Element Seqn Id St Int Rpt
LVHNAQNLMQSVKETVREAE VINC_CHICK 1022 15 -
LVHNAQNLMQSVKETVREAE MUSVINCULX 1023 15 -
LVGNAQNLMQSVKETVRAAE DMC103B413 881 13 -
LVHNAQNLMQSVKETVREAE VINC_PIG 293 15 -
LVHNAQNLMQSVKDVVRAAE VINC_CAEEL 966 24 -
LVHNAQNLMQSVKETVREAE VINC_HUMAN 1022 15 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
DELAPPKPPLP VINC_CHICK 855 855 -
DELAPPKPPLP VINC_MOUSE 855 855 -
DELAPPKPPLP VINC_HUMAN 855 855 -
AERAPPRPPLP O46037 750 750 -
AERAPPRPPLP Q24584 750 750 -
DIPAPPRPPPP VINC_CAEEL 784 784 -
DIPTPPRPPPP Q17162 767 767 -

Motif 2 width=14
Element Seqn Id St Int Rpt
PPPRPPPPEEKDEE VINC_CHICK 870 4 -
PPPRPPPPEEKDEE VINC_MOUSE 870 4 -
PPPRPPPPEEKDEE VINC_HUMAN 870 4 -
APVRPPPPETDDED O46037 765 4 -
PVRRPPPPETDDED Q24584 766 5 -
PPPRPPPPPEYDEE VINC_CAEEL 799 4 -
PPPRPPPPPETDDE Q17162 782 4 -

Motif 3 width=18
Element Seqn Id St Int Rpt
NDIIAAAKRMALLMAEMS VINC_CHICK 916 32 -
NDIIAAAKRMALLMAEMS VINC_MOUSE 916 32 -
NDIIAAAKRMALLMAEMS VINC_HUMAN 916 32 -
NEIIAAAKRMAILMARLS O46037 811 32 -
NEIIAAAKRMAILMARLS Q24584 812 32 -
NDIVAAAKRMAILMARLS VINC_CAEEL 851 38 -
NEIVAAAKRMAILMARLS Q17162 834 38 -

Motif 4 width=16
Element Seqn Id St Int Rpt
KDIAKASDEVTRLAKE VINC_CHICK 951 17 -
KDIAKASDEVTRLAKE VINC_MOUSE 951 17 -
KDIAKASDEVTRLAKE VINC_HUMAN 951 17 -
KKIAEASEDVTRLAKE O46037 847 18 -
KKIAEASEDVTRLAKE Q24584 848 18 -
KAIADSSEEVTRLAVQ VINC_CAEEL 886 17 -
KAIADSSEEVTRLAVQ Q17162 869 17 -

Motif 5 width=16
Element Seqn Id St Int Rpt
EVAKQCTDKRIRTNLL VINC_CHICK 966 -1 -
EVAKQCTDKRIRTNLL VINC_MOUSE 966 -1 -
EVAKQCTDKRIRTNLL VINC_HUMAN 966 -1 -
ELARQCTDRRIRTNLL O46037 862 -1 -
ELARQCTDRRIRTNLL Q24584 863 -1 -
QLARLCTDIKMRTALL VINC_CAEEL 901 -1 -
QLARQCTVIKMRMTLL Q17162 884 -1 -

Motif 6 width=22
Element Seqn Id St Int Rpt
ERIPTISTQLKILSTVKATMLG VINC_CHICK 985 3 -
ERIPTISTQLKILSTVKATMLG VINC_MOUSE 985 3 -
ERIPTISTQLKILSTVKATMLG VINC_HUMAN 985 3 -
ERIPTIGTQLKILSTVKATMLG O46037 881 3 -
ERIPTIGTQLKILSTVKATMLG Q24584 882 3 -
ERIPTIATQLKVLSTVKATMLG VINC_CAEEL 920 3 -
ERIPTIATQLKILSTVKATMLG Q17162 903 3 -

Motif 7 width=20
Element Seqn Id St Int Rpt
LVHNAQNLMQSVKETVREAE VINC_CHICK 1022 15 -
LVHNAQNLMQSVKETVREAE VINC_MOUSE 1022 15 -
LVHNAQNLMQSVKETVREAE VINC_HUMAN 1022 15 -
LVGNAQNLMQSVKETVRAAE O46037 916 13 -
LVGNAQNLMQSVKETVRAAE Q24584 917 13 -
LVHNAQNLMQSVKDVVRAAE VINC_CAEEL 966 24 -
LVLNAQNLMQSVKDTVRAAE Q17162 949 24 -