| Literature References | 1. OTTO, J.J.
vinculin.
CELL MOTIL.CYTOSKELETON 16 1-6 (1990).
2. JONES, P., JACKSON, P., PRICE, G.J., PATEL, B., OHANION, V., LEAR, A.L.
AND CRITCHLEY, D.R.
Identification of a talin binding site in the cytoskeletal protein vinculin.
J.CELL BIOL. 109 2917-2927 (1989).
3. HERRENKNECHT, K., OZAWA, M., ECKERSKORN, C., LOTTSPEICH, F., LENTER, M.
AND KEMLER, R.
The uvomorulin-anchorage protein alpha-catenin is a vinculin homolog.
PROC.NATL.ACAD.SCI.U.S.A. 88 9156-9160 (1991).
4. WELLER, P.A., OGRYZKO, E.P., CORBEN, E.B., ZHIDKOVA, N.I., PATEL, B.,
PRICE, G.J., SPURR, N.K., KOTELIANSKY, V.E. AND CRITCHLEY, D.R.
Complete sequence of human vinculin and assignment of the gene to
chromosome 10.
PROC.NATL.ACAD.SCI.U.S.A. 87 5667-5671 (1990).
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| Documentation | Vinculin is a eukaryotic protein that appears to be involved in the
attachment of actin-based microfilaments to the plasma membrane [1].
It also interacts with other structural proteins, such as talin [2] and
alpha-actinin. The protein is located on the cytoplasmic side of focal
contacts or adhesion plaques.
Vinculin is a large protein (~1000 residues) that contains an acidic
N-terminal domain, separated from a smaller, basic C-terminal domain by a
50-residue proline-rich region [3]. The central part of the N-terminal
domain consists of a variable number of repeats of a 110-residue domain,
one of which is lacking in nematode vinculin [4]. Alpha-catenins are
evolutionarily related to vinculin, but differ in terms of their structure
in lacking the repeated domain and proline-rich segment.
VINCULIN is a 7-element fingerprint that provides a signature for
vinculins. The fingerprint was derived from an initial alignment of 6
sequences: the motifs were drawn from conserved regions spanning the
C-terminal half of the alignment - motifs 1 and 2 lie in the proline-rich
region; and motifs 3-7 lie in the C-terminal domain. Two iterations on
OWL30.0 were required to reach convergence, at which point a true set
comprising 8 sequences was identified. Two partial matches were also found,
S87175S3 and VINC_XENLA, both of which are vinculin fragments.
An update on SPTR37_9f identified a true set of 7 sequences, and 9
partial matches.
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