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PR00784

Identifier
MTUNCOUPLING  [View Relations]  [View Alignment]  
Accession
PR00784
No. of Motifs
6
Creation Date
22-AUG-1997  (UPDATE 28-JUL-1999)
Title
Mitochondrial brown fat uncoupling protein signature
Database References

PROSITE; PS00215 MITOCH_CARRIER
BLOCKS; BL00215
INTERPRO; IPR002030
Literature References
1.CASSARD, A.M., BOUILLAUD, F., MATTEI, M.G., HENTZ, E., RAIMBAULT, S.,
THOMAS, M. AND RICQUIER, D. 
Human uncoupling protein gene: structure, comparison with rat gene, and
assignment to the long arm of chromosome 4. 
J.CELL BIOCHEMISTRY 43(3) 255-264 (1990).
 
2. AQUILA, H., LINK, T.A. AND KLINGENBERG, M.
THe uncoupling protein from brown fat mitochondria is related to the
mitochondrial ADP/ATP carrier. analysis of sequence homologies and of
folding of the protein in the membrane. 
EMBO J. 4(9) 2369-2376 (1985).
 
3. KOZAK, L.P., BRITTON, J.H., KOZAK, U.C. AND WELLS, J.M.
The mitochondrial uncoupling protein gene. Correlation of exon structure
to transmembrane domains. 
J.BIOL.CHEM. 263(25) 12274-12277 (1988).

Documentation
The brown fat uncoupling protein (UCP) dissipates oxidative energy into heat
by transporting protons from the cytosol into the mitochondrial matrix [1].
UCP functions as a dimer, forming a proton channel that leads to uncoupling
of oxidative phosphorylation by dissipation of the electrochemical potential
across the inner membrane.
 
The protein has a tripartite structure, with 3 similar ~100 residue domains.
The domains exhibit striking conservation of several residues, especially
of glycine and proline, which may constitute structurally strategic
positions [2]. The protein is thought to contain 6 transmembrane helices
[3]. These characteristics are shared by the ADP/ATP carrier protein, which
is a member of the mitochondrial carrier family. 
 
MTUNCOUPLING is a 6-element fingerprint that provides a signature for
mitochondrial brown fat uncoupling proteins. The fingerprint was derived
from an initial alignment of 6 sequences: the motifs were drawn from
conserved regions spanning the full alignment length - motif 1 lies in
the first putative TM region; motif 2 spans part of the fifth putative
TM region; and motif 6 lies at the C-terminal extremity, in the purine
nucleotide binding domain. Two iterations on OWL29.4 were required to
reach convergence, at which point a true set comprising 11 sequences was
identified.  
 
An update on SPTR37_9f identified a true set of 13 sequences, and 4
partial matches.
Summary Information
  13 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
4 codes involving 2 elements
Composite Feature Index
6131313131313
5000000
4000000
3000000
2000431
123456
True Positives
O77792        O88285        UCP1_HUMAN    UCP1_MESAU    
UCP1_MOUSE UCP1_RABIT UCP1_RAT UCP2_HUMAN
UCP2_MOUSE UCP2_RAT UCP3_HUMAN UCP3_MOUSE
UCP3_RAT
True Positive Partials
Codes involving 2 elements
O24391 O43808 O65623 O81845
Sequence Titles
O77792      MITOCHONDRIAL UNCOUPLING PROTEIN 3 (UCP 3) - BOS TAURUS (BOVINE). 
O88285 UNCOUPLING PROTEIN-2 - MUS MUSCULUS (MOUSE).
UCP1_HUMAN MITOCHONDRIAL BROWN FAT UNCOUPLING PROTEIN 1 (UCP 1) - HOMO SAPIENS (HUMAN).
UCP1_MESAU MITOCHONDRIAL BROWN FAT UNCOUPLING PROTEIN 1 (UCP 1) - MESOCRICETUS AURATUS (GOLDEN HAMSTER).
UCP1_MOUSE MITOCHONDRIAL BROWN FAT UNCOUPLING PROTEIN 1 (UCP 1) - MUS MUSCULUS (MOUSE).
UCP1_RABIT MITOCHONDRIAL BROWN FAT UNCOUPLING PROTEIN 1 (UCP 1) - ORYCTOLAGUS CUNICULUS (RABBIT).
UCP1_RAT MITOCHONDRIAL BROWN FAT UNCOUPLING PROTEIN 1 (UCP 1) - RATTUS NORVEGICUS (RAT).
UCP2_HUMAN MITOCHONDRIAL UNCOUPLING PROTEIN 2 (UCP 2) (UCPH) - HOMO SAPIENS (HUMAN).
UCP2_MOUSE MITOCHONDRIAL UNCOUPLING PROTEIN 2 (UCP 2) (UCPH) - MUS MUSCULUS (MOUSE).
UCP2_RAT MITOCHONDRIAL UNCOUPLING PROTEIN 2 (UCP 2) - RATTUS NORVEGICUS (RAT).
UCP3_HUMAN MITOCHONDRIAL UNCOUPLING PROTEIN 3 (UCP 3) - HOMO SAPIENS (HUMAN).
UCP3_MOUSE MITOCHONDRIAL UNCOUPLING PROTEIN 3 (UCP 3) - MUS MUSCULUS (MOUSE).
UCP3_RAT MITOCHONDRIAL UNCOUPLING PROTEIN 3 (UCP 3) - RATTUS NORVEGICUS (RAT).

O24391 MITOCHONDRIAL UNCOUPLING PROTEIN - SOLANUM TUBEROSUM (POTATO).
O43808 PEROXISOMAL INTEGRAL MEMBRANE PROTEIN - HOMO SAPIENS (HUMAN).
O65623 PLANT UNCOUPLING MITOCHONDRIAL PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O81845 MITOCHONDRIAL UNCOUPLING PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Scan History
OWL29_4    2  100  NSINGLE    
SPTR37_9f 3 100 NSINGLE
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
FSAGVAACLADII UCP_MESAU 17 17 -
FSAGVAACLADVI UCP_RABIT 18 18 -
FSAGVAACVADII UCP_BOVIN 2 2 -
FSAGVSACLADII UCP_MOUSE 17 17 -
FSAGVSACLADII UCP_RAT 17 17 -
FSAPIAACLADVI UCP_HUMAN 18 18 -

Motif 2 width=20
Element Seqn Id St Int Rpt
IQGEGQISSTIRYKGVLGTI UCP_MESAU 42 12 -
IQGEFPITSGIRYKGVLGTI UCP_RABIT 43 12 -
IQGECLISSAIRYKGVLGTI UCP_BOVIN 27 12 -
IQGEGQASSTIRYKGVLGTI UCP_MOUSE 42 12 -
IQGEGQASSTIRYKGVLGTI UCP_RAT 42 12 -
VQGECPTSSVIRYKGVLGTI UCP_HUMAN 43 12 -

Motif 3 width=13
Element Seqn Id St Int Rpt
YDTVQEYFSSGKE UCP_MESAU 95 33 -
YDTVQEFFTSGEE UCP_RABIT 96 33 -
YDTVQEFFTTGKE UCP_BOVIN 80 33 -
YDSVQEYFSSGRE UCP_MOUSE 95 33 -
YDTVQEYFSSGRE UCP_RAT 95 33 -
YDTVQEFLTAGKE UCP_HUMAN 96 33 -

Motif 4 width=18
Element Seqn Id St Int Rpt
IATTESFSTLWKGTTPNL UCP_MESAU 163 55 -
IATTESLTSLWKGTTPNL UCP_RABIT 163 54 -
IATTEGLTGLWKGTSPNL UCP_BOVIN 146 53 -
IATTESLSTLWKGTTPNL UCP_MOUSE 163 55 -
IATTESLSTLWKGTTPNL UCP_RAT 163 55 -
IATTEGLTGLWKGTTPNL UCP_HUMAN 164 55 -

Motif 5 width=19
Element Seqn Id St Int Rpt
ILADDVPCHLLSAFVAGFC UCP_MESAU 206 25 -
ILADDVPCHFVSALIAGFC UCP_RABIT 206 25 -
LLADDVPCHFVSAVVAGFC UCP_BOVIN 189 25 -
ILADDVPCHLLSALVAGFC UCP_MOUSE 206 25 -
ILADDVPCHLLSALVAGFC UCP_RAT 206 25 -
ILADDVPCHLVSALIAGFC UCP_HUMAN 207 25 -

Motif 6 width=22
Element Seqn Id St Int Rpt
IMFVCFEQLKKELSKSRQTVDC UCP_MESAU 283 58 -
IMFVCFEKLKGELMRSRQTVDC UCP_RABIT 283 58 -
IMFVCFERLKQELMKCRHTMDC UCP_BOVIN 265 57 -
IMFVCFEQLKKELMKSRQTVDC UCP_MOUSE 283 58 -
IMFVCFEQLKKELMKSRQTVDC UCP_RAT 283 58 -
IMFVCFEQLKRELSKSRQTMDC UCP_HUMAN 284 58 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
LGAGTAACIADLI UCP2_MOUSE 18 18 -
LGAGTAACIADLI O88285 18 18 -
LGAGTAACIADLI UCP2_RAT 18 18 -
LGAGTAACIADLI UCP2_HUMAN 18 18 -
LGAGTAACFADLL UCP3_MOUSE 18 18 -
LGAGTAACFADLL UCP3_RAT 18 18 -
LGAGTAACFADLV UCP3_HUMAN 18 18 -
LAAGTAACFADLL O77792 18 18 -
FSAGVSACLADII UCP1_MOUSE 17 17 -
FSAGVAACLADVI UCP1_RABIT 18 18 -
FSAGVAACLADII UCP1_MESAU 17 17 -
FSAPIAACLADVI UCP1_HUMAN 18 18 -
FSAGVSACLADII UCP1_RAT 17 17 -

Motif 2 width=20
Element Seqn Id St Int Rpt
SQGLVRTAASAQYRGVLGTI UCP2_MOUSE 47 16 -
SQGLVRTAASAQYRGVLGTI O88285 47 16 -
SQGLARTAASAQYRGVLGTI UCP2_RAT 47 16 -
SQGPVRATASAQYRGVMGTI UCP2_HUMAN 47 16 -
IQGENPGAQSVQYRGVLGTI UCP3_MOUSE 43 12 -
IQGENPGVQSVQYRGVLGTI UCP3_RAT 43 12 -
ENQAVQTARLVQYRGVLGTI UCP3_HUMAN 46 15 -
ENQAALAARSAQYRGVLGTI O77792 46 15 -
IQGEGQASSTIRYKGVLGTI UCP1_MOUSE 42 12 -
IQGEFPITSGIRYKGVLGTI UCP1_RABIT 43 12 -
IQGEGQISSTIRYKGVLGTI UCP1_MESAU 42 12 -
VQGECPTSSVIRYKGVLGTI UCP1_HUMAN 43 12 -
IQGEGQASSTIRYKGVLGTI UCP1_RAT 42 12 -

Motif 3 width=13
Element Seqn Id St Int Rpt
YDSVKQFYTKGSE UCP2_MOUSE 100 33 -
YDSVKQFYTKGSE O88285 100 33 -
YDSVKQFYTKGSE UCP2_RAT 100 33 -
YDSVKQFYTKGSE UCP2_HUMAN 100 33 -
YDSVKQFYTPKGA UCP3_MOUSE 96 33 -
YDSVKQFYTPKGT UCP3_RAT 96 33 -
YDSVKQVYTPKGA UCP3_HUMAN 99 33 -
YDSVKQFYTPKGS O77792 99 33 -
YDSVQEYFSSGRE UCP1_MOUSE 95 33 -
YDTVQEFFTSGEE UCP1_RABIT 96 33 -
YDTVQEYFSSGKE UCP1_MESAU 95 33 -
YDTVQEFLTAGKE UCP1_HUMAN 96 33 -
YDTVQEYFSSGRE UCP1_RAT 95 33 -

Motif 4 width=18
Element Seqn Id St Int Rpt
IAREEGIRGLWKGTSPNV UCP2_MOUSE 166 53 -
IAREEGIRGLWKGTSPNV O88285 166 53 -
IAREEGIRGLWKGTSPNV UCP2_RAT 166 53 -
IAREEGFRGLWKGTSPNV UCP2_HUMAN 166 53 -
IAREEGVRGLWKGTWPNI UCP3_MOUSE 165 56 -
IAREEGVRGLWKGTWPNI UCP3_RAT 165 56 -
IAREEGVRGLWKGTLPNI UCP3_HUMAN 169 57 -
IAREEGVRGLWKGILPNI O77792 168 56 -
IATTESLSTLWKGTTPNL UCP1_MOUSE 163 55 -
IATTESLTSLWKGTTPNL UCP1_RABIT 163 54 -
IATTESFSTLWKGTTPNL UCP1_MESAU 163 55 -
IATTEGLTGLWKGTTPNL UCP1_HUMAN 164 55 -
IATTESLSTLWKGTTPNL UCP1_RAT 163 55 -

Motif 5 width=19
Element Seqn Id St Int Rpt
LMTDDLPCHFTSAFGAGFC UCP2_MOUSE 209 25 -
LMTDDLPCHFTSAFGAGFC O88285 209 25 -
LMTDDLPCHFTSAFGAGFC UCP2_RAT 209 25 -
LMTDDLPCHFTSAFGAGFC UCP2_HUMAN 209 25 -
LFTDNFPCHFVSAFGAGFC UCP3_MOUSE 208 25 -
LFTDNFPCHFVSAFGAGFC UCP3_RAT 208 25 -
LLTDNFPCHFVSAFGAGFC UCP3_HUMAN 212 25 -
LLTDNFPCHFVSAFGAGFC O77792 211 25 -
ILADDVPCHLLSALVAGFC UCP1_MOUSE 206 25 -
ILADDVPCHFVSALIAGFC UCP1_RABIT 206 25 -
ILADDVPCHLLSAFVAGFC UCP1_MESAU 206 25 -
ILADDVPCHLVSALIAGFC UCP1_HUMAN 207 25 -
ILADDVPCHLLSALVAGFC UCP1_RAT 206 25 -

Motif 6 width=22
Element Seqn Id St Int Rpt
VMFVTYEQLKRALMAAYQSREA UCP2_MOUSE 286 58 -
VMFVTYEQLKRALMAAYQSREA O88285 286 58 -
VMFVTYEQLKRALMAAYESREA UCP2_RAT 286 58 -
VMFVTYEQLKRALMAACTSREA UCP2_HUMAN 286 58 -
MMFVTYEQLKRALMKVQVLRES UCP3_MOUSE 285 58 -
MMFVTYEQLKRALMKVQVLRES UCP3_RAT 285 58 -
VMFVTYEQLKRALMKVQMLRES UCP3_HUMAN 289 58 -
VMFVTYEQMKRALMKVQMLRDS O77792 288 58 -
IMFVCFEQLKKELMKSRQTVDC UCP1_MOUSE 283 58 -
IMFVCFEKLKGELMRSRQTVDC UCP1_RABIT 283 58 -
IMFVCFEQLKKELSKSRQTVDC UCP1_MESAU 283 58 -
IMFVCFEQLKRELSKSRQTMDC UCP1_HUMAN 284 58 -
IMFVCFEQLKKELMKSRQTVDC UCP1_RAT 283 58 -