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PR00772

Identifier
ENTEROTOXINB  [View Relations]  [View Alignment]  
Accession
PR00772
No. of Motifs
5
Creation Date
26-AUG-1997  (UPDATE 07-JUN-1999)
Title
Heat labile enterotoxin B chain signature
Database References

PFAM; PF01376 Enterotoxin_B
INTERPRO; IPR001835
PDB; 1LTB
SCOP; 1LTB
Literature References
1. SIXMA, T.K., KALK, K.H., VAN ZANTEN, B.A., DAUTER, Z., KINGMA, J.,
WITHOLT, B. AND HOL, W.G.
Refined structure of Escherichia coli heat-labile enterotoxin, a close
relative of cholera toxin. 
J.MOL.BIOL. 230(3) 890-918 (1993).

Documentation
E.coli heat-labile enterotoxin is a bacterial protein toxin with an AB5
multimer structure, in which the B pentamer has a membrane-binding function
and the A subunit is needed for enzymatic activity [1]. The B subunits are
arranged as a donut-shaped pentamer, each subunit participating in ~30
hydrogen bonds and 6 salt bridges with its two neighbours [1]. 
 
The A subunit has a less well-defined secondary structure. It predominantly
interacts with the pentamer via the C-terminal A2 fragment, which runs
through the charged central pore of the B subunits. A putative catalytic
residue in the A1 fragment (Glu112) lies close to a hydrophobic region,
which packs two loops together. It is thought that this region might be
important for catalysis and membrane translocation [1]. 
 
ENTEROTOXINB is a 5-element fingerprint that provides a signature for
heat labile enterotoxin B chains. The fingerprint was derived from an
initial alignment of 4 sequences: the motifs were drawn from conserved
regions spanning the full alignment length - motif 1 spans helix 1 and
strand 1; motif 2 spans strands 2 and 3; motif 3 includes strand 4 and
the N-terminus of helix 2; motif 4 includes the C-terminus of helix 2
and strand 5; and motif 5 encodes strand 6. Two iterations on OWL29.4
were required to reach convergence, at which point a true set comprising
11 sequences was identified.
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
555555
400000
300000
200000
12345
True Positives
CHTB_VIBCH    ELBH_ECOLI    ELBP_ECOLI    Q56635        
Q57193
Sequence Titles
CHTB_VIBCH  CHOLERA ENTEROTOXIN, BETA CHAIN PRECURSOR - VIBRIO CHOLERAE. 
ELBH_ECOLI HEAT-LABILE ENTEROTOXIN B CHAIN PRECURSOR (LT-B, HUMAN) (LTH-B) - ESCHERICHIA COLI.
ELBP_ECOLI HEAT-LABILE ENTEROTOXIN B CHAIN PRECURSOR (LT-B, PORCINE) (LTP-B) - ESCHERICHIA COLI.
Q56635 CHOLERA TOXIN PRECURSOR - VIBRIO CHOLERAE.
Q57193 CHOLERA TOXIN B PROTEIN (CTB) PRECURSOR (CTB) - VIBRIO CHOLERAE.
Scan History
OWL29_4    2  50   NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
TDLCAEYHNTQIHTLNNKIFSY NRL_1CHBD 6 6 -
TELCSEYRNTQIYTINDKILSY ECOELT1 27 27 -
TELCSEYRNTQIYTINDKILSY ELBP_ECOLI 27 27 -
TELCSEYHNTQIYTINDKILSY ELBH_ECOLI 27 27 -
TDLCAEYHNTQIYTLNDKIFSY CHTB_VIBCH 27 27 -
TDLCAEYHNTQIHTLNDKILSY VIBCTXABA1 27 27 -
TDLCAEYHNTQIYTLNDKIFSY NRL_1XTCD 6 6 -
TDLCAESHNTQIYTLNDKIFSY VCCTX1 27 27 -
TDLCAEYHNTQIHTLNDKIFSY VCTOXAB561 27 27 -
TDLCAEYHNTQIHTLNDKIFSY A00483 17 17 -
TDLCAEYHNTQIHTLNDKIFSY A09803 34 34 -

Motif 2 width=17
Element Seqn Id St Int Rpt
ESLAGKREMAIITFKNG VCCTX1 50 1 -
ESMAGKREMVIITFKSG ECOELT1 50 1 -
ESLAGKREMAIITFKNG VCTOXAB561 50 1 -
ESMAGKREMVIITFKSG ELBP_ECOLI 50 1 -
ESMAGKREMVIITFKSG ELBH_ECOLI 50 1 -
ESLAGKREMAIITFKNG CHTB_VIBCH 50 1 -
ESLAGKREMAIITFKNG A00483 40 1 -
ESLAGKREMAIITFKNG A09803 57 1 -
ESLAGKREMAIITFKNG NRL_1CHBD 29 1 -
ESLAGNREMAIITFKNG VIBCTXABA1 50 1 -
ESLAGKREMAIITFKNG NRL_1XTCD 29 1 -

Motif 3 width=20
Element Seqn Id St Int Rpt
FQVEVPGSQHIDSQKKAIER ELBP_ECOLI 69 2 -
FQVEVPGSQHIDSQKKAIER ECOELT1 69 2 -
FQVEVPGSQHIDSQKKAIER VIBCTXABA1 69 2 -
FQVEVPSSQHIDSQKKAIER NRL_1XTCD 48 2 -
FQVEVPSSQHIDSQKKAIER VCCTX1 69 2 -
FQVEVPGSQHIDSQKKAIER VCTOXAB561 69 2 -
FQVEVPGSQHIDSQKKAIER A00483 59 2 -
FQVEVPGSQHIDSQKKAIER A09803 76 2 -
FQVEVPGSQHIDSQKKAIER NRL_1CHBD 48 2 -
FQVEVPGSQHIDSQKKAIER CHTB_VIBCH 69 2 -
FQVEVPGSQHIDSQKKAIER ELBH_ECOLI 69 2 -

Motif 4 width=20
Element Seqn Id St Int Rpt
KDTLRIAYLTEAKVEKLCTW NRL_1XTCD 69 1 -
KDTLRIAYLTEAKVEKLCVW VIBCTXABA1 90 1 -
KDTLRIAYLTEAKVEKLCVW A09803 97 1 -
KDTLRIAYLTEAKVEKLCVW A00483 80 1 -
KDTLRITYLTETKIDKLCVW ECOELT1 90 1 -
KDTLRIAYLTEAKVEKLCVW CHTB_VIBCH 90 1 -
KDTLRITYLTETKIDKLCVW ELBH_ECOLI 90 1 -
KDTLRITYLTETKIDKLCVW ELBP_ECOLI 90 1 -
KDTLRIAYLTEAKVEKLCVW VCTOXAB561 90 1 -
KNTLRIAYLTEAKVEKLCVW NRL_1CHBD 69 1 -
KDTLRIAYLTEAKVEKLCVW VCCTX1 90 1 -

Motif 5 width=14
Element Seqn Id St Int Rpt
WNNKTPNSIAAISM ECOELT1 109 -1 -
WNNKTPHAIAAISM NRL_1XTCD 88 -1 -
WNNKTPHAIAAISM VCCTX1 109 -1 -
WNNKTPHAIAAISM VCTOXAB561 109 -1 -
WNNKTPHAIAAISM A00483 99 -1 -
WNNKTPHAIAAISM A09803 116 -1 -
WNNKTPHAIAAISM NRL_1CHBD 88 -1 -
WNNKTPNSIAAISM ELBH_ECOLI 109 -1 -
WNNKTPNSIAAISM ELBP_ECOLI 109 -1 -
WNNKTPHAIAAISM CHTB_VIBCH 109 -1 -
WNNKTPHAIAAISM VIBCTXABA1 109 -1 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
TDLCAEYHNTQIYTLNDKIFSY CHTB_VIBCH 27 27 -
TDLCAEYHNTQIHTLNDKIFSY Q57193 27 27 -
TDLCAEYHNTQIHTLNDKILSY Q56635 27 27 -
TELCSEYHNTQIYTINDKILSY ELBH_ECOLI 27 27 -
TELCSEYRNTQIYTINDKILSY ELBP_ECOLI 27 27 -

Motif 2 width=17
Element Seqn Id St Int Rpt
ESLAGKREMAIITFKNG CHTB_VIBCH 50 1 -
ESLAGKREMAIITFKNG Q57193 50 1 -
ESLAGNREMAIITFKNG Q56635 50 1 -
ESMAGKREMVIITFKSG ELBH_ECOLI 50 1 -
ESMAGKREMVIITFKSG ELBP_ECOLI 50 1 -

Motif 3 width=20
Element Seqn Id St Int Rpt
FQVEVPGSQHIDSQKKAIER CHTB_VIBCH 69 2 -
FQVEVPGSQHIDSQKKAIER Q57193 69 2 -
FQVEVPGSQHIDSQKKAIER Q56635 69 2 -
FQVEVPGSQHIDSQKKAIER ELBH_ECOLI 69 2 -
FQVEVPGSQHIDSQKKAIER ELBP_ECOLI 69 2 -

Motif 4 width=20
Element Seqn Id St Int Rpt
KDTLRIAYLTEAKVEKLCVW CHTB_VIBCH 90 1 -
KDTLRIAYLTEAKVEKLCVW Q57193 90 1 -
KDTLRIAYLTEAKVEKLCVW Q56635 90 1 -
KDTLRITYLTETKIDKLCVW ELBH_ECOLI 90 1 -
KDTLRITYLTETKIDKLCVW ELBP_ECOLI 90 1 -

Motif 5 width=14
Element Seqn Id St Int Rpt
WNNKTPHAIAAISM CHTB_VIBCH 109 -1 -
WNNKTPHAIAAISM Q57193 109 -1 -
WNNKTPHAIAAISM Q56635 109 -1 -
WNNKTPNSIAAISM ELBH_ECOLI 109 -1 -
WNNKTPNSIAAISM ELBP_ECOLI 109 -1 -