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PR00764

Identifier
COMPLEMENTC9  [View Relations]  [View Alignment]  
Accession
PR00764
No. of Motifs
6
Creation Date
24-AUG-1997  (UPDATE 07-JUN-1999)
Title
Complement C9 signature
Database References

PROSITE; PS00279 MAC_PERFORIN
BLOCKS; BL00279
INTERPRO; IPR001862
Literature References
1. MARAZZITI, D., EGGERTSEN, G., FEY, G.H. AND STANLEY, K.K. 
Relationships between the gene and protein structure in human complement
component C9. 
BIOCHEMISTRY 27(17) 6529-6534 (1988).
 
2. STANLEY, K.K., KOCHER, H.P., LUZIO, J.P., JACKSON, P. AND TSCHOPP, J.
The sequence and topology of human complement component C9.
EMBO J. 4(2) 375-382 (1985).
 
3. PEITSCH, M.C., AMIGUET, P., GUY, R., BRUNNER, J., MAIZEL, J.V.JR.
AND TSCHOPP, J.
Localization and molecular modelling of the membrane-inserted domain of
the ninth component of human complement and perforin.
MOL.IMMUNOL. 27(7) 589-602 (1990).
 
4. DISCIPIO, R.G., GEHRING, M.R., PODACK, E.R., KAN, C.C., HUGLI, T.E.
AND FEY, G.H.
Nucleotide sequence of cdna and derived amino acid sequence of human
complement component C9. 
PROC.NATL.ACAD.SCI.U.S.A. 81(23) 7298-7302 (1984). 

Documentation
Complement component C9 is a multi-domain protein that contains an
N-terminal type-1 TSP domain, an LDL-receptor class A repeat, a number of
potential transmembrane (TM) regions and a C-terminal EGF-like domain [1-3].
Hydropathy analysis of the sequence indicates the N-terminal half of C9 to
be predominantly hydrophilic in character, while the C-terminal section
is more hydrophobic. The amphipathic organisation of the primary structure
is consistent with the known potential of polymerised C9 to penetrate lipid
bilayers, causing the formation of transmembrane channels [3,4]. 
 
COMPLEMENTC9 is a 6-element fingerprint that provides a signature for the
complement C9 family of proteins. The fingerprint was derived from an
initial alignment of 5 sequences: the motifs were drawn from conserved
regions spanning the full alignment length - motif 1 lies within the
N-terminal type-1 TSP motif; motif 2 was drawn from the LDL-receptor
class A repeat; motif 3 spans part of the first putative TM domain and
includes the region encoded by PROSITE pattern MAC_PERFORIN (PS00279);
and motif 6 lies in the C-terminal EGF-like region. Two iterations on
OWL29.4 were required to reach convergence, at which point a true set
comprising 18 sequences was identified. A single partial match was found,
C8B_RAT, a complement C8 beta chain fragment that lacks the first 3 motifs.
 
An update on SPTR37_9f identified a true set of 12 sequences.
Summary Information
12 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6121212121212
5000000
4000000
3000000
2000000
123456
True Positives
CO6_HUMAN     CO7_HUMAN     CO8A_HUMAN    CO8A_RABIT    
CO8B_HUMAN CO8B_RABIT CO9_FUGRU CO9_HORSE
CO9_HUMAN CO9_RABIT CO9_RAT Q62957
Sequence Titles
CO6_HUMAN   Complement component C6 precursor - Homo sapiens (Human). 
CO7_HUMAN Complement component C7 precursor - Homo sapiens (Human).
CO8A_HUMAN Complement component C8 alpha chain precursor - Homo sapiens (Human).
CO8A_RABIT Complement component C8 alpha chain precursor - Oryctolagus cuniculus (Rabbit).
CO8B_HUMAN Complement component C8 beta chain precursor - Homo sapiens (Human).
CO8B_RABIT Complement component C8 beta chain precursor - Oryctolagus cuniculus (Rabbit).
CO9_FUGRU Complement component C9 precursor - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes).
CO9_HORSE COMPLEMENT COMPONENT C9 PRECURSOR - EQUUS CABALLUS (HORSE).
CO9_HUMAN COMPLEMENT COMPONENT C9 PRECURSOR - HOMO SAPIENS (HUMAN).
CO9_RABIT COMPLEMENT COMPONENT C9 PRECURSOR - ORYCTOLAGUS CUNICULUS (RABBIT).
CO9_RAT COMPLEMENT COMPONENT C9 PRECURSOR - RATTUS NORVEGICUS (RAT).
Q62957 COMPLEMENT COMPONENT C9 PRECURSOR - RATTUS NORVEGICUS (RAT).
Scan History
OWL29_4    2  100  NSINGLE    
SPTR37_9f 2 13 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
FRSRSIEVFGQFNGKRC CO9_HUMAN 62 62 -
FRSRSIEVFGQFHGKSC CO9_RABIT 62 62 -
FRSRSIEVFGQFNGQRC CO9_HORSE 62 62 -
FRSRSILAFGQFNGKSC CO9_MOUSE 40 40 -
HRSRSVEVFGQFGGKPC CO9_ONCMY 38 38 -

Motif 2 width=21
Element Seqn Id St Int Rpt
FQCGTGRCIKKRLLCNGDNDC CO9_HORSE 105 26 -
FTCESGACIKLRLSCNGDYDC CO9_ONCMY 84 29 -
FQCETGRCIKRRLLCNGDNDC CO9_MOUSE 83 26 -
FHCGTGRCIKRRLLCNGDNDC CO9_RABIT 106 27 -
FQCSTGRCIKMRLRCNGDNDC CO9_HUMAN 105 26 -

Motif 3 width=23
Element Seqn Id St Int Rpt
KSLPTTYEKGEYFAFLETYGTHY CO9_RABIT 329 202 -
KALPTSYEKGEYFGFLETYGTHY CO9_MOUSE 302 198 -
KSLPLEYEKGQYFSFLEDYGTHY CO9_ONCMY 294 189 -
KALPTAYEKGEYIAFLETYGTHY CO9_HORSE 324 198 -
KALPTTYEKGEYFAFLETYGTHY CO9_HUMAN 324 198 -

Motif 4 width=20
Element Seqn Id St Int Rpt
NWASSLNDAPVLISQRLSPI CO9_HORSE 457 110 -
NWASSINDAPVLISQKLSPI CO9_HUMAN 457 110 -
NWASSLANAPALISQRMSPI CO9_MOUSE 440 115 -
NWARTVGDAPALLSSEPEPI CO9_ONCMY 428 111 -
NWASSLSDAPVLINQKLSPI CO9_RABIT 467 115 -

Motif 5 width=20
Element Seqn Id St Int Rpt
AYIKKQNLEKAVEDYIDEFS CO9_MOUSE 470 10 -
AHQKKQNLERAIEDYINEFS CO9_HORSE 487 10 -
AHQKRQNLERGIEDYINEFS CO9_RABIT 497 10 -
AHLKKQNLERAIEDYINEFS CO9_HUMAN 487 10 -
ANTRRLNMQRATQEYEAEYS CO9_ONCMY 458 10 -

Motif 6 width=21
Element Seqn Id St Int Rpt
CHPCQNGGTVIQIDGQCLCSC CO9_HORSE 510 3 -
CHTCQNGGTVILMDGKCLCAC CO9_HUMAN 510 3 -
CSPCQNGGTALLMDGQCLCTC CO9_RABIT 520 3 -
CYPCLNGGTIILLDGQCLCSC CO9_MOUSE 493 3 -
CKPCHNGGSLALLDGKCLCLC CO9_ONCMY 481 3 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
FRSRSIEVFGQFQGKSC CO9_RAT 60 60 -
FRSRSMEVFGQFQGKSC Q62957 73 73 -
FRSRSIEVFGQFNGQRC CO9_HORSE 62 62 -
FRSRSIEVFGQFHGKSC CO9_RABIT 62 62 -
FRSRSIEVFGQFNGKRC CO9_HUMAN 62 62 -
YRHRSLLQPNKFGGTIC CO8A_HUMAN 58 58 -
YRHRSLLQPNKFGGTIC CO8A_RABIT 58 58 -
RRSRGVEVFGQFAGIAC CO9_FUGRU 56 56 -
YRYAYLLQPSQFHGEPC CO8B_HUMAN 84 84 -
SKVRSVLRPSQFGGQPC CO6_HUMAN 101 101 -
TRRRSVAVYGQYGGQPC CO7_HUMAN 47 47 -
YRHAYLLRPSQFNGEPC CO8B_RABIT 84 84 -

Motif 2 width=21
Element Seqn Id St Int Rpt
FQCETGRCIKRKLLCNGDNDC CO9_RAT 103 26 -
FQCETGRCIKRKLLCNGDNDC Q62957 116 26 -
FQCGTGRCIKKRLLCNGDNDC CO9_HORSE 105 26 -
FHCGTGRCIKRRLLCNGDNDC CO9_RABIT 106 27 -
FQCSTGRCIKMRLRCNGDNDC CO9_HUMAN 105 26 -
QCKETGRCLKRHLVCNGDQDC CO8A_HUMAN 101 26 -
QCKETGRCLKRHLVCNGENDC CO8A_RABIT 101 26 -
FQCESGSCIKLRLKCNGDYDC CO9_FUGRU 101 28 -
VCAQTGRCVNRRLLCNGDNDC CO8B_HUMAN 126 25 -
FRCDSGRCIARKLECNGENDC CO6_HUMAN 144 26 -
FRCFSGQCISKSLVCNGDSDC CO7_HUMAN 89 25 -
VCAQTGRCVNRRLLCNGDNDC CO8B_RABIT 126 25 -

Motif 3 width=23
Element Seqn Id St Int Rpt
KALPVSYEKGEYFGFLETYGTHY CO9_RAT 326 202 -
KALPVSYEKGEYFGFLETYGTHY Q62957 339 202 -
KALPTAYEKGEYIAFLETYGTHY CO9_HORSE 324 198 -
KSLPTTYEKGEYFAFLETYGTHY CO9_RABIT 329 202 -
KALPTTYEKGEYFAFLETYGTHY CO9_HUMAN 324 198 -
MELPDQYNYGMYAKFINDYGTHY CO8A_HUMAN 319 197 -
VELPEQYNYGMYSKFINDYGTHY CO8A_RABIT 319 197 -
KSLPLEYEKGIYYAFLEDYGTHY CO9_FUGRU 307 185 -
KRLPLEYSYGEYRDLFRDFGTHY CO8B_HUMAN 322 175 -
NHLPLEYNSALYSRIFDDFGTHY CO6_HUMAN 343 178 -
SHLPSLYDYSAYRRLIDQYGTHY CO7_HUMAN 281 171 -
QRVPLEYSYGEYRDLFRDFGHHF CO8B_RABIT 322 175 -

Motif 4 width=20
Element Seqn Id St Int Rpt
NWASSLDDAPALISQKLSPI CO9_RAT 466 117 -
NWASSLDDAPALISQKLSPI Q62957 479 117 -
NWASSLNDAPVLISQRLSPI CO9_HORSE 457 110 -
NWASSLSDAPVLINQKLSPI CO9_RABIT 467 115 -
NWASSINDAPVLISQKLSPI CO9_HUMAN 457 110 -
SWGRSLKYNPVVIDFEMQPI CO8A_HUMAN 445 103 -
FWGRSLKYNPVVIDFEMQPI CO8A_RABIT 446 104 -
NWARTIASAPALINSEPEPI CO9_FUGRU 441 111 -
EWGDAVQYNPAIIKVKVEPL CO8B_HUMAN 451 106 -
EWLESVKENPAVIDFELAPI CO6_HUMAN 471 105 -
AWAESVTNLPQVIKQKLTPL CO7_HUMAN 405 101 -
EWGDAVQYNPAIIKIKVEPL CO8B_RABIT 451 106 -

Motif 5 width=20
Element Seqn Id St Int Rpt
AYAKKQNMEKAIEDYVNEFS CO9_RAT 496 10 -
AYAKKQNMEKAIEDYVNEFS Q62957 509 10 -
AHQKKQNLERAIEDYINEFS CO9_HORSE 487 10 -
AHQKRQNLERGIEDYINEFS CO9_RABIT 497 10 -
AHLKKQNLERAIEDYINEFS CO9_HUMAN 487 10 -
LEAKRQNLRRALDQYLMEFN CO8A_HUMAN 476 11 -
VEAKRQNLRRALDQYLMEFN CO8A_RABIT 477 11 -
ANSRIANLKQATADYVAEYN CO9_FUGRU 471 10 -
SSTVRQNMKQALEEFQKEVS CO8B_HUMAN 482 11 -
AVTKRNNLRKALQEYAAKFD CO6_HUMAN 500 9 -
ASVKKLYLKWALEEYLDEFD CO7_HUMAN 434 9 -
SSTVKQNMRQALEEFQGEVS CO8B_RABIT 482 11 -

Motif 6 width=21
Element Seqn Id St Int Rpt
CYPCQNGGTAILLDGQCMCSC CO9_RAT 519 3 -
CYPCQNGGTAILLDGQCMCSC Q62957 532 3 -
CHPCQNGGTVIQIDGQCLCSC CO9_HORSE 510 3 -
CSPCQNGGTALLMDGQCLCTC CO9_RABIT 520 3 -
CHTCQNGGTVILMDGKCLCAC CO9_HUMAN 510 3 -
CGPCFNNGVPILEGTSCRCQC CO8A_HUMAN 499 3 -
CGPCFNNGKPILEGTSCRCQC CO8A_RABIT 500 3 -
CRPCHNGGTLALLDGKCICMC CO9_FUGRU 494 3 -
CAPCQGNGVPVLKGSRCDCIC CO8B_HUMAN 505 3 -
CAPCPNNGRPTLSGTECLCVC CO6_HUMAN 523 3 -
CRPCQNGGLATVEGTHCLCHC CO7_HUMAN 457 3 -
CAPCQGNGVPVQKGSRCDCIC CO8B_RABIT 505 3 -