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PR00763

Identifier
COAGULIN  [View Relations]  [View Alignment]  
Accession
PR00763
No. of Motifs
8
Creation Date
26-AUG-1997  (UPDATE 07-JUN-1999)
Title
Coagulin signature
Database References

INTERPRO; IPR000275
Literature References
1. SRIMAL, S., MIYATA, T., KAWABATA, S., MIYATA, T. AND IWANAGA, S.
The complete amino acid sequence of coagulogen isolated from southeast
asian horseshoe crab, Carcinoscorpius rotundicauda.
J.BIOCHEMISTRY 98(2) 305-318 (1985). 
 
2. MIYATA, T., USUI, K. AND IWANAGA, S.
The amino acid sequence of coagulogen isolated from southeast asian
horseshoe crab, Tachypleus gigas. 
J.BIOCHEMISTRY 95(6) 1793-1801 (1984).

Documentation
Coagulogen is a gel-forming protein of hemolymph that hinders the spread of
invaders by immobilising them [1,2]. The protein contains a single 175-
residue polypeptide chain; this is cleaved after Arg-18 and Arg-46 by a
clotting enzyme contained in the hemocyte and activated by a bacterial
endotoxin (lipopolysaccharide). Cleavage releases two chains of coagulin,
A and B, linked by two disulphide bonds, together with the peptide C [1,2].
Gel formation results from interlinking of coagulin molecules.
 
Secondary structure prediction suggests the C peptide forms an alpha-
helix, which is released during the proteolytic conversion of coagulogen to
coagulin gel [1]. The beta-sheet structure and 16 half-cystines found in the
molecule appear to yield a compact protein stable to acid and heat.
 
COAGULIN is an 8-element fingerprint that provides a signature for
coagulins. The fingerprint was derived from an initial alignment of 4
sequences: the motifs were drawn from conserved regions spanning the full
alignment length - motif 1 includes the A chain; motif 2 lies in the C
peptide; and motifs 3-8 reside in the B chain. A single iteration on
OWL29.4 was required to reach convergence, no further sequences being
identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
844444444
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
COAG_CARRO    COAG_LIMPO    COAG_TACGI    COAG_TACTR    
Sequence Titles
COAG_CARRO  COAGULOGEN [CONTAINS: COAGULIN; PEPTIDE C] - CARCINOSCORPIUS ROTUNDICAUDA (SOUTHEAST ASIAN HORSESHOE CRAB). 
COAG_LIMPO COAGULOGEN PRECURSOR [CONTAINS: COAGULIN; PEPTIDE C] - LIMULUS POLYPHEMUS (ATLANTIC HORSESHOE CRAB).
COAG_TACGI COAGULOGEN [CONTAINS: COAGULIN; PEPTIDE C] - TACHYPLEUS GIGAS (SOUTHEAST ASIAN HORSESHOE CRAB).
COAG_TACTR COAGULOGEN PRECURSOR [CONTAINS: COAGULIN; PEPTIDE C] - TACHYPLEUS TRIDENTATUS (JAPANESE HORSESHOE CRAB).
Scan History
OWL29_4    1  100  NSINGLE    
SPTR37_9f 2 9 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
DTNAPLCLCDEPGILGR COAG_CARRO 2 2 -
DTNAPICLCDEPGVLGR COAG_TACTR 22 22 -
DTNAPLCLCDEPGILGR COAG_TACGI 2 2 -
DPNVPTCLCEEPTLLGR COAG_LIMPO 22 22 -

Motif 2 width=20
Element Seqn Id St Int Rpt
IEKAVEAVAEESGVSGRGFS COAG_CARRO 30 11 -
IEKAVEAVAQESGVSGRGFS COAG_TACTR 50 11 -
IEKAVEEVAKEGGVSGRGFS COAG_TACGI 30 11 -
IEEAVQAITDKDEISGRGFS COAG_LIMPO 50 11 -

Motif 3 width=18
Element Seqn Id St Int Rpt
FSHHPVFRECGKYECRTV COAG_CARRO 51 1 -
FSHHPVFRECGKYECRTV COAG_TACTR 71 1 -
FSHHPVFRECGKYECRTV COAG_TACGI 51 1 -
FGGHPAFKECGKYECRTV COAG_LIMPO 71 1 -

Motif 4 width=21
Element Seqn Id St Int Rpt
EHTRCYNFPPFVHFTSECPVS COAG_CARRO 71 2 -
EHSRCYNFPPFTHFKLECPVS COAG_TACTR 91 2 -
EHSRCYNFPPFIHFKSECPVS COAG_TACGI 71 2 -
EDSRCYNFFPFHHFPSECPVS COAG_LIMPO 91 2 -

Motif 5 width=14
Element Seqn Id St Int Rpt
CEPVFGYTVAGEFR COAG_CARRO 95 3 -
CEPVFGYTVAGEFR COAG_TACTR 115 3 -
CEPVFGYTAAGEFR COAG_TACGI 95 3 -
CEPTFGYTTSNELR COAG_LIMPO 115 3 -

Motif 6 width=21
Element Seqn Id St Int Rpt
RVIVQAPRAGFRQCVWQHKCR COAG_CARRO 108 -1 -
RVIVQAPRAGFRQCVWQHKCR COAG_TACTR 128 -1 -
RVIVQAPRAGFRQCVWQHKCR COAG_TACGI 108 -1 -
RIIVQAPKAGFRQCVWQHKCR COAG_LIMPO 128 -1 -

Motif 7 width=21
Element Seqn Id St Int Rpt
CGFSGRCTQQRSVVRLVTYNL COAG_CARRO 134 5 -
CGYNGRCTQQRSVVRLVTYNL COAG_TACTR 154 5 -
CGFNGRCTQQRSVVRLVTFNL COAG_TACGI 134 5 -
CQRTGRCTQQRSVVRLVTYDL COAG_LIMPO 155 6 -

Motif 8 width=19
Element Seqn Id St Int Rpt
EKDGFLCESFRTCCGCPCR COAG_CARRO 155 0 -
EKDGFLCESFRTCCGCPCR COAG_TACTR 175 0 -
EKNGFLCETFRTCCGCPCR COAG_TACGI 155 0 -
EKGVFFCENVRTCCGCPCR COAG_LIMPO 176 0 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
DTNAPLCLCDEPGILGR COAG_CARRO 2 2 -
DTNAPICLCDEPGVLGR COAG_TACTR 22 22 -
DTNAPLCLCDEPGILGR COAG_TACGI 2 2 -
DPNVPTCLCEEPTLLGR COAG_LIMPO 22 22 -

Motif 2 width=20
Element Seqn Id St Int Rpt
IEKAVEAVAEESGVSGRGFS COAG_CARRO 30 11 -
IEKAVEAVAQESGVSGRGFS COAG_TACTR 50 11 -
IEKAVEEVAKEGGVSGRGFS COAG_TACGI 30 11 -
IEEAVQAITDKDEISGRGFS COAG_LIMPO 50 11 -

Motif 3 width=18
Element Seqn Id St Int Rpt
FSHHPVFRECGKYECRTV COAG_CARRO 51 1 -
FSHHPVFRECGKYECRTV COAG_TACTR 71 1 -
FSHHPVFRECGKYECRTV COAG_TACGI 51 1 -
FGGHPAFKECGKYECRTV COAG_LIMPO 71 1 -

Motif 4 width=21
Element Seqn Id St Int Rpt
EHTRCYNFPPFVHFTSECPVS COAG_CARRO 71 2 -
EHSRCYNFPPFTHFKLECPVS COAG_TACTR 91 2 -
EHSRCYNFPPFIHFKSECPVS COAG_TACGI 71 2 -
EDSRCYNFFPFHHFPSECPVS COAG_LIMPO 91 2 -

Motif 5 width=14
Element Seqn Id St Int Rpt
CEPVFGYTVAGEFR COAG_CARRO 95 3 -
CEPVFGYTVAGEFR COAG_TACTR 115 3 -
CEPVFGYTAAGEFR COAG_TACGI 95 3 -
CEPTFGYTTSNELR COAG_LIMPO 115 3 -

Motif 6 width=21
Element Seqn Id St Int Rpt
RVIVQAPRAGFRQCVWQHKCR COAG_CARRO 108 -1 -
RVIVQAPRAGFRQCVWQHKCR COAG_TACTR 128 -1 -
RVIVQAPRAGFRQCVWQHKCR COAG_TACGI 108 -1 -
RIIVQAPKAGFRQCVWQHKCR COAG_LIMPO 128 -1 -

Motif 7 width=21
Element Seqn Id St Int Rpt
CGFSGRCTQQRSVVRLVTYNL COAG_CARRO 134 5 -
CGYNGRCTQQRSVVRLVTYNL COAG_TACTR 154 5 -
CGFNGRCTQQRSVVRLVTFNL COAG_TACGI 134 5 -
CQRTGRCTQQRSVVRLVTYDL COAG_LIMPO 155 6 -

Motif 8 width=19
Element Seqn Id St Int Rpt
EKDGFLCESFRTCCGCPCR COAG_CARRO 155 0 -
EKDGFLCESFRTCCGCPCR COAG_TACTR 175 0 -
EKNGFLCETFRTCCGCPCR COAG_TACGI 155 0 -
EKGVFFCENVRTCCGCPCR COAG_LIMPO 176 0 -