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PR00752

Identifier
VASOPRSNV1AR  [View Relations]  [View Alignment]  
Accession
PR00752
No. of Motifs
7
Creation Date
09-SEP-1997  (UPDATE 10-JUN-1999)
Title
Vasopressin V1A receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00896 VASOPRESSINR
INTERPRO; IPR001224
GCRDB; GCR_0360; GCR_0942
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Vasopressin and oxytocin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.284-291.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Vasopressin and oxytocin are members of the neurohypophyseal hormone family
found in all mammalian species [6]. They are present in high levels in the
posterior pituitary. Vasopressin has an essential role in the control of 
the water content of the body, acting in the kidney to increase water and
sodium absorption [6]. In higher concentrations, vasopressin stimulates
contraction of vascular smooth muscle, stimulates glycogen breakdown in the
liver, induces platelet activation, and evokes release of corticotrophin 
from the anterior pituitary [6]. Vasopressin and its analogues are used
clinically to treat diabetes insipidus [6].
 
In the periphery, the V1A receptor is found in high levels in vascular
smooth muscle, myometrium and the bladder where it mediates contraction [6]. 
In the CNS, V1 sites are distributed widely and are found in lateral septal
nucleus, hippocampus, superior collicular, substantia nigra and central
grey matter [6]. The receptors activate phosphoinositide metabolism through
a pertussis-toxin-insensitive G protein, probably of the Gq/G11 class [6].
 
VASOPRSNV1AR is a 7-element fingerprint that provides a signature for
vasopressin V1A receptors. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from conserved sections 
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the vasopressin V1A receptors but
distinguish them from the rest of the rhodopsin-like superfamily - motifs 1
and 2 lie at the N-terminus; motif 3 spans the first cytoplasmic loop and
the N-terminus of TM domain 2; motif 4 spans the second cytoplasmic loop
and the N-terminus of TM domain 4; motif 5 lies in the third cytoplasmic
loop; and motifs 6 and 7 lie at the C-terminus. A single iteration on
OWL29.4 was required to reach convergence, no further sequences being
identified beyond the starting set. Three partial matches were also found:
I50132 is a vasotocin receptor that matches motifs 3, 4 and 6; V1BR_HUMAN
and V1BR_RAT are vasopressin V1B receptors that match motifs 3 and 4. 
 
An update on SPTR37_9f identified a true set of 4 sequences, and 2
partial matches.
Summary Information
   4 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
74444444
60000000
50000000
40000000
30000000
20022000
1234567
True Positives
V1AR_HUMAN    V1AR_MOUSE    V1AR_RAT      V1AR_SHEEP    
True Positive Partials
Codes involving 2 elements
Q90352 V1BR_HUMAN
Sequence Titles
V1AR_HUMAN  Vasopressin V1a receptor (V1aR) (Vascular/hepatic-type arginine vasopressin receptor) (Antidiuretic hormone receptor 1a) (AVPR V1a) - Homo sapiens (Human). 
V1AR_MOUSE Vasopressin V1a receptor (V1aR) (Vascular/hepatic-type arginine vasopressin receptor) (Antidiuretic hormone receptor 1a) (AVPR V1a) - Mus musculus (Mouse).
V1AR_RAT Vasopressin V1a receptor (V1aR) (Vascular/hepatic-type arginine vasopressin receptor) (Antidiuretic hormone receptor 1a) (AVPR V1a) - Rattus norvegicus (Rat).
V1AR_SHEEP Vasopressin V1a receptor (V1aR) (Vascular/hepatic-type arginine vasopressin receptor) (Antidiuretic hormone receptor 1a) (AVPR V1a) - Ovis aries (Sheep).

Q90352 VASOSTOCIN-RECEPTOR - CATOSTOMUS COMMERSONI (WHITE SUCKER).
V1BR_HUMAN Vasopressin V1b receptor (V1bR) (AVPR V1b) (Vasopressin V3 receptor) (AVPR V3) (Antidiuretic hormone receptor 1b) - Homo sapiens (Human).
Scan History
OWL29_4    1  100  NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
GSPSPGPSNSSRWWPL V1AR_SHEEP 5 5 -
GPDAGPSGNSSPWWPL V1AR_HUMAN 6 6 -
GSQDRSVGNSSPWWPL V1AR_RAT 6 6 -
GSHDLPAGNSSPWWPL MUSV1AAVRB 6 6 -
GSQDRSVGNSSPWWPL S71837 6 6 -
GSHDLPAGNSSPWWPL MUSV1AAVRA 6 6 -

Motif 2 width=18
Element Seqn Id St Int Rpt
SQEAARLGEGDSPLGDVR S71837 29 7 -
SREAAGLGEGGSPPGDVR MUSV1AAVRA 29 7 -
SREAAGLGEGGSRRGDVR MUSV1AAVRB 29 7 -
SQEAARLGEGDSPLGDVR V1AR_RAT 29 7 -
SREAEALGEGNGPPRDVR V1AR_HUMAN 29 7 -
SGDLAGLGEDGGPQADTR V1AR_SHEEP 28 7 -

Motif 3 width=16
Element Seqn Id St Int Rpt
LHRTPRKTSRMHLFIR S71837 76 29 -
LHRTPRKTSRMHLFIR V1AR_SHEEP 75 29 -
LHRTPRKTSRMHLFIR V1AR_HUMAN 76 29 -
LHRTPRKTSRMHLFIR V1AR_RAT 76 29 -
LHRTPRKTSRMHLFIR MUSV1AAVRA 76 29 -
LHRTPRKTSRMHLFIR MUSV1AAVRB 76 29 -

Motif 4 width=18
Element Seqn Id St Int Rpt
HPLKTLQQPARRSRLMIA S71837 155 63 -
HPLKTLQQPARRSRLMIA V1AR_RAT 155 63 -
HPLKTLQQPARRSRLMIA V1AR_HUMAN 155 63 -
HPLKTLQQPARRSRLMIA V1AR_SHEEP 154 63 -
HPLKTLQQPARRSRLMIA MUSV1AAVRA 155 63 -
HPLKTLQQPARRSRLMIA MUSV1AAVRB 155 63 -

Motif 5 width=14
Element Seqn Id St Int Rpt
AFQKGFLLAPCVSS V1AR_HUMAN 265 92 -
ALYRGVLHARCVSS V1AR_SHEEP 265 93 -
PFHKGLLVTPCVSS MUSV1AAVRA 270 97 -
PFHKGLLVTPCVSS V1AR_RAT 271 98 -
PFHKGLLVTPCVSS S71837 271 98 -
PFHKGLLVTPCVSS MUSV1AAVRB 270 97 -

Motif 6 width=11
Element Seqn Id St Int Rpt
LLQDCVQSFPC MUSV1AAVRB 360 76 -
LLQDCVQSFPC MUSV1AAVRA 360 76 -
LLQDCVQSFPC V1AR_HUMAN 355 76 -
LLQDCAQSFPC V1AR_SHEEP 355 76 -
LLQDCVQSFPC V1AR_RAT 361 76 -
LLQDCVQSFPC S71837 361 76 -

Motif 7 width=16
Element Seqn Id St Int Rpt
CHSMAQKFAKDDSDSM V1AR_RAT 372 0 -
CQSIAQKFAKDDSDSM MUSV1AAVRB 371 0 -
CHSMAQKFAKDDSDSM S71837 372 0 -
CQSIAQKFAKDDSDSM MUSV1AAVRA 371 0 -
CQNVKRTFTREGSDSM V1AR_SHEEP 366 0 -
CQNMKEKFNKEDTDSM V1AR_HUMAN 366 0 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
GSHDLPAGNSSPWWPL V1AR_MOUSE 6 6 -
GSQDRSVGNSSPWWPL V1AR_RAT 6 6 -
GPDAGPSGNSSPWWPL V1AR_HUMAN 6 6 -
GSPSPGPSNSSRWWPL V1AR_SHEEP 5 5 -

Motif 2 width=18
Element Seqn Id St Int Rpt
SREAAGLGEGGSPPGDVR V1AR_MOUSE 29 7 -
SQEAARLGEGDSPLGDVR V1AR_RAT 29 7 -
SREAEALGEGNGPPRDVR V1AR_HUMAN 29 7 -
SGDLAGLGEDGGPQADTR V1AR_SHEEP 28 7 -

Motif 3 width=16
Element Seqn Id St Int Rpt
LHRTPRKTSRMHLFIR V1AR_MOUSE 76 29 -
LHRTPRKTSRMHLFIR V1AR_RAT 76 29 -
LHRTPRKTSRMHLFIR V1AR_HUMAN 76 29 -
LHRTPRKTSRMHLFIR V1AR_SHEEP 75 29 -

Motif 4 width=18
Element Seqn Id St Int Rpt
HPLKTLQQPARRSRLMIA V1AR_MOUSE 155 63 -
HPLKTLQQPARRSRLMIA V1AR_RAT 155 63 -
HPLKTLQQPARRSRLMIA V1AR_HUMAN 155 63 -
HPLKTLQQPARRSRLMIA V1AR_SHEEP 154 63 -

Motif 5 width=14
Element Seqn Id St Int Rpt
PFHKGLLVTPCVSS V1AR_MOUSE 270 97 -
PFHKGLLVTPCVSS V1AR_RAT 271 98 -
AFQKGFLLAPCVSS V1AR_HUMAN 265 92 -
ALYRGVLHARCVSS V1AR_SHEEP 265 93 -

Motif 6 width=11
Element Seqn Id St Int Rpt
LLQDCVQSFPC V1AR_MOUSE 360 76 -
LLQDCVQSFPC V1AR_RAT 361 76 -
LLQDCVQSFPC V1AR_HUMAN 355 76 -
LLQDCAQSFPC V1AR_SHEEP 355 76 -

Motif 7 width=16
Element Seqn Id St Int Rpt
CQSIAQKFAKDDSDSM V1AR_MOUSE 371 0 -
CHSMAQKFAKDDSDSM V1AR_RAT 372 0 -
CQNMKEKFNKEDTDSM V1AR_HUMAN 366 0 -
CQNVKRTFTREGSDSM V1AR_SHEEP 366 0 -