| Literature References | 1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
5. STOLTZFUS, L.J., SOSA-PINEDA, B., MOSKOWITZ, S.M., MENON, K.P.,
DLOTT, B., HOOPER, L., TEPLOW, D.B., SHULL, R.M. AND NEUFELD, E.F.
Cloning and characterization of cDNA encoding canine alpha-L-iduronidase.
mRNA deficiency in mucopolysaccharidosis I dog.
J.BIOL.CHEM. 267(10) 6570-6575 (1992).
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| Documentation | O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 39 encompasses mammalian alpha-L-iduronidases and some
bacterial beta-xylosidases.
Alpha-L-iduronidase is a lysosomal enzyme, deficiency of which causes muco-
polysaccharidosis I (MPS I) [5]. The enzyme hydrolyses alpha-L-iduronosidic
linkages in desulphated dermatan.
GLHYDRLASE39 is a 7-element fingerprint that provides a signature for
family 39 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions
spanning the central portion of the alignment - motif 3 includes the
region encoded by PROSITE pattern GLYCOSYL_HYDROL_F39 (PS01027), which
contains a Glu residue that may act as the proton donor in the catalytic
mechanism. A single iteration on OWL29.3 was required to reach convergence,
no further sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 6 sequences, and 1
partial match.
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| Sequence Titles | IDUA_CANFA ALPHA-L-IDURONIDASE PRECURSOR (EC 3.2.1.76) - CANIS FAMILIARIS (DOG).
IDUA_HUMAN ALPHA-L-IDURONIDASE PRECURSOR (EC 3.2.1.76) - HOMO SAPIENS (HUMAN).
IDUA_MOUSE ALPHA-L-IDURONIDASE PRECURSOR (EC 3.2.1.76) - MUS MUSCULUS (MOUSE).
O30360 BETA-XYLOSIDASE - THERMOANAEROBACTERIUM SP. 'JW/SL YS485'.
XYNB_CALSA BETA-XYLOSIDASE (EC 3.2.1.37) (1,4-BETA-D-XYLAN XYLOHYDROLASE) (XYLAN 1,4-BETA-XYLOSIDASE) - CALDOCELLUM SACCHAROLYTICUM (CALDICELLULOSIRUPTOR SACCHAROLYTICUS).
XYNB_THESA BETA-XYLOSIDASE (EC 3.2.1.37) (1,4-BETA-D-XYLAN XYLOHYDROLASE) (XYLAN 1,4-BETA-XYLOSIDASE) - THERMOANAEROBACTER SACCHAROLYTICUM.
O30428 XYLANASE - CALDOCELLUM SACCHAROLYTICUM (CALDICELLULOSIRUPTOR SACCHAROLYTICUS).
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