| Literature References | 1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
5. KOPP, M., MUELLER, H. AND HOLZER, H.
Molecular analysis of the neutral trehalase gene from Saccharomyces
cerevisiae.
J.BIOL.CHEM. 268 4766-4774 (1993).
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| Documentation | O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt).
Family 37 encompasses trehalases. These enzymes are responsible for
degradation of the disaccharide alpha,alpha-trehalose, yielding two glucose
units [5]:
alpha,alpha-trehalose + H(2)O = 2 D-glucose
GLHYDRLASE37 is a 7-element fingerprint that provides a signature for
family 37 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 8 sequences: the motifs were drawn from conserved regions
spanning the C-terminal half of the alignment - motif 6 includes the
region encoded by PROSITE pattern TREHALASE_2(PS00928). Two iterations
on OWL29.3 were required to reach convergence, at which point a true set
comprising 14 sequences was identified. Three partial matches were also
found, all of which are fragments.
An update on SPTR37_9f identified a true set of 19 sequences, and 1
partial match.
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| Sequence Titles | O22986 TREHALASE PRECUSOR ISOLOG - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O44736 F57B10.7 PROTEIN - CAENORHABDITIS ELEGANS.
Q22195 SIMILAR TO TREHALASE PRECURSOR. NCBI GI: 1086612 - CAENORHABDITIS ELEGANS.
Q23176 W05E10.4 PROTEIN - CAENORHABDITIS ELEGANS.
Q27453 TREHALASE (EC 3.2.1.28) - BOMBYX MORI (SILK MOTH).
TREA_BOMMO TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - BOMBYX MORI (SILK MOTH).
TREA_CANAL NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - CANDIDA ALBICANS (YEAST).
TREA_ECOLI PERIPLASMIC TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - ESCHERICHIA COLI.
TREA_HUMAN TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - HOMO SAPIENS (HUMAN).
TREA_KLULA NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - KLUYVEROMYCES LACTIS (YEAST).
TREA_RABIT TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - ORYCTOLAGUS CUNICULUS (RABBIT).
TREA_SCHPO NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
TREA_TENMO TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - TENEBRIO MOLITOR (YELLOW MEALWORM).
TREA_YEAST NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
TREB_EMENI NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - EMERICELLA NIDULANS (ASPERGILLUS NIDULANS).
TREB_MAGGR NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - MAGNAPORTHE GRISEA (RICE BLAST FUNGUS).
TREB_NEUCR NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - NEUROSPORA CRASSA.
TREB_YEAST PROBABLE TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
TREF_ECOLI PROBABLE CYTOPLASMIC TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - ESCHERICHIA COLI.
Q19472 F15A2.2 PROTEIN - CAENORHABDITIS ELEGANS.
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