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PR00744

Identifier
GLHYDRLASE37  [View Relations]  [View Alignment]  
Accession
PR00744
No. of Motifs
7
Creation Date
12-JUN-1997  (UPDATE 07-JUN-1999)
Title
Glycosyl hydrolase family 37 signature
Database References

PROSITE; PS00927 TREHALASE_1; PS00928 TREHALASE_2
BLOCKS; BL00927
PFAM; PF01204 Trehalase
INTERPRO; IPR001661
Literature References
1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
 
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
5. KOPP, M., MUELLER, H. AND HOLZER, H.
Molecular analysis of the neutral trehalase gene from Saccharomyces
cerevisiae.
J.BIOL.CHEM. 268 4766-4774 (1993).

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). 
 
Family 37 encompasses trehalases. These enzymes are responsible for
degradation of the disaccharide alpha,alpha-trehalose, yielding two glucose
units [5]:
   		alpha,alpha-trehalose + H(2)O = 2 D-glucose
 
GLHYDRLASE37 is a 7-element fingerprint that provides a signature for
family 37 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 8 sequences: the motifs were drawn from conserved regions 
spanning the C-terminal half of the alignment - motif 6 includes the
region encoded by PROSITE pattern TREHALASE_2(PS00928). Two iterations
on OWL29.3 were required to reach convergence, at which point a true set
comprising 14 sequences was identified. Three partial matches were also
found, all of which are fragments.
 
An update on SPTR37_9f identified a true set of 19 sequences, and 1
partial match.
Summary Information
  19 codes involving  7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
719191919191919
61011111
50000000
40000000
30000000
20000000
1234567
True Positives
O22986        O44736        Q22195        Q23176        
Q27453 TREA_BOMMO TREA_CANAL TREA_ECOLI
TREA_HUMAN TREA_KLULA TREA_RABIT TREA_SCHPO
TREA_TENMO TREA_YEAST TREB_EMENI TREB_MAGGR
TREB_NEUCR TREB_YEAST TREF_ECOLI
True Positive Partials
Codes involving 6 elements
Q19472
Sequence Titles
O22986      TREHALASE PRECUSOR ISOLOG - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS). 
O44736 F57B10.7 PROTEIN - CAENORHABDITIS ELEGANS.
Q22195 SIMILAR TO TREHALASE PRECURSOR. NCBI GI: 1086612 - CAENORHABDITIS ELEGANS.
Q23176 W05E10.4 PROTEIN - CAENORHABDITIS ELEGANS.
Q27453 TREHALASE (EC 3.2.1.28) - BOMBYX MORI (SILK MOTH).
TREA_BOMMO TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - BOMBYX MORI (SILK MOTH).
TREA_CANAL NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - CANDIDA ALBICANS (YEAST).
TREA_ECOLI PERIPLASMIC TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - ESCHERICHIA COLI.
TREA_HUMAN TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - HOMO SAPIENS (HUMAN).
TREA_KLULA NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - KLUYVEROMYCES LACTIS (YEAST).
TREA_RABIT TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - ORYCTOLAGUS CUNICULUS (RABBIT).
TREA_SCHPO NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
TREA_TENMO TREHALASE PRECURSOR (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - TENEBRIO MOLITOR (YELLOW MEALWORM).
TREA_YEAST NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
TREB_EMENI NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - EMERICELLA NIDULANS (ASPERGILLUS NIDULANS).
TREB_MAGGR NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - MAGNAPORTHE GRISEA (RICE BLAST FUNGUS).
TREB_NEUCR NEUTRAL TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - NEUROSPORA CRASSA.
TREB_YEAST PROBABLE TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
TREF_ECOLI PROBABLE CYTOPLASMIC TREHALASE (EC 3.2.1.28) (ALPHA,ALPHA-TREHALASE) (ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE) - ESCHERICHIA COLI.

Q19472 F15A2.2 PROTEIN - CAENORHABDITIS ELEGANS.
Scan History
OWL29_3    2  100  NSINGLE    
SPTR37_9f 2 24 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
YGKILNANRSYYLCRSQPP TREA_KLULA 344 344 -
YGKILNANRSYYLCRSQPP TREA_YEAST 341 341 -
YGHIPNGGRVYYLQRSQPP TREA_RABIT 207 207 -
YSKILNANRSYYLCRSQPP TREB_YEAST 370 370 -
FGHIPNGSRWYYQERSQPP TREA_BOMMO 200 200 -
YGHIPNGNRSYYLSRSQPP TREA_ECOLI 191 191 -
YGHIPNGNRTYYLSRSQPP TREF_ECOLI 207 207 -
YGFIPNGARVFYLNRSQPP TREA_TENMO 203 203 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LSCYHPDGIGIPPETEPG TREA_KLULA 414 51 -
LSRYHPNGLGIPPETESD TREA_YEAST 411 51 -
LNRYHVPYGGPRPESYSK TREA_RABIT 275 49 -
LSCYHSDGIGIPPETEPD TREB_YEAST 440 51 -
LLRYYIPSAGPRPESYYE TREA_BOMMO 268 49 -
LNRYWDDRDTPRPESWVE TREA_ECOLI 266 56 -
LNRYWDDRDTPRDESWLE TREF_ECOLI 281 55 -
LAQYNSNSGSPRPESYYE TREA_TENMO 271 49 -

Motif 3 width=18
Element Seqn Id St Int Rpt
FLHDRGVRESGHDTTYRF TREA_KLULA 469 37 -
FLHDRGVRESGHDTTYRF TREA_YEAST 466 37 -
WAELKAGAESGWDFSSRW TREA_RABIT 309 16 -
FLHDRAVRESGHDTTYRF TREB_YEAST 495 37 -
YADLKSAAESGWDFSTRW TREA_BOMMO 303 17 -
YRDLRSAAASGWDFSSRW TREA_ECOLI 300 16 -
YRDLRAGAASGWDYSSRW TREF_ECOLI 314 15 -
YMDLKSAAESGWDFSSRW TREA_TENMO 306 17 -

Motif 4 width=18
Element Seqn Id St Int Rpt
LATIDLNSLLYKYEVDIA TREA_KLULA 493 6 -
LATIDLNSLLYKYEIDIA TREA_YEAST 490 6 -
LVPVDLNAFLCQAEELLS TREA_RABIT 344 17 -
LATIDLNSLLYKYEKDIA TREB_YEAST 519 6 -
VIPVDLNAIFAGALQITA TREA_BOMMO 340 19 -
IVPVDLNSLMFKMEKILA TREA_ECOLI 332 14 -
FIPIDLNAFLFKLESAIA TREF_ECOLI 346 14 -
IIPVDLNAFLCQAFQKLS TREA_TENMO 343 19 -

Motif 5 width=17
Element Seqn Id St Int Rpt
IDKYLWDEETGFYYDYN TREA_KLULA 545 34 -
ITKYMWDDESGFFFDYN TREA_YEAST 543 35 -
LTALLWDEDKGAWFDYD TREA_RABIT 387 25 -
INKYMWDEDSGFFFYYN TREB_YEAST 572 35 -
IEQALWDEEDGVWHDYD TREA_BOMMO 383 25 -
IEKYLWNDQQGWYADYD TREA_ECOLI 375 25 -
VNRYLWDDENGIYRDYD TREF_ECOLI 389 25 -
IEMVHYNRDDGIWYDWD TREA_TENMO 386 25 -

Motif 6 width=17
Element Seqn Id St Int Rpt
SLDRPTRQWDYPFGWAP TREA_KLULA 618 56 -
SISRPIRQWDYPFGWAP TREA_YEAST 616 56 -
SLQNTGQQWDFPNAWAP TREA_RABIT 454 50 -
SIDRPIRQWDYPFGWAP TREB_YEAST 645 56 -
SLVNSGEQWDFPNAWPP TREA_BOMMO 453 53 -
TSVKSGQQWDAPNGWAP TREA_ECOLI 439 47 -
SEYETGEQWDKPNGWAP TREF_ECOLI 452 46 -
SLSHTGEQWDYPNAWPP TREA_TENMO 456 53 -

Motif 7 width=14
Element Seqn Id St Int Rpt
EGFGWVNSSYLLGM TREA_KLULA 706 71 -
EGFGWVNASYILGL TREA_YEAST 704 71 -
EGFGWTNGVALMLL TREA_RABIT 533 62 -
EGFGWVNTSYLLGL TREB_YEAST 733 71 -
DGFGWSNGVVLEFL TREA_BOMMO 533 63 -
DGFGWTNGVTLKML TREA_ECOLI 516 60 -
DGFGWTNGVVRRLI TREF_ECOLI 530 61 -
SGFGWTNGVVLEFI TREA_TENMO 536 63 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
YGKILNATRSYYLCRSQPP TREB_MAGGR 318 318 -
YGKILNANRSYYLTRSQPP TREB_EMENI 328 328 -
YGKILNATRSYYLCRSQPP TREB_NEUCR 325 325 -
YGKILNANRSYYLGRSQPP TREA_CANAL 490 490 -
YGKILNANRSYYLCRSQPP TREA_KLULA 344 344 -
YGKILNANRSYYLCRSQPP TREA_YEAST 341 341 -
YGKILNANRTYYLLRSQPP TREA_SCHPO 322 322 -
YGHIPNGGRVYYLQRSQPP TREA_RABIT 207 207 -
YSKILNANRSYYLCRSQPP TREB_YEAST 370 370 -
FGHIPNGSRWYYQERSQPP TREA_BOMMO 200 200 -
YGHVPNGGRVYYLQRSQPP TREA_HUMAN 207 207 -
FGHIPNGSRWYYQERSQPP Q27453 200 200 -
YGHIPNGNRSYYLSRSQPP TREA_ECOLI 191 191 -
YGFIPNGTRVYYLNRSQPP Q22195 179 179 -
YGYALNGARAYYTNRSQPP O22986 202 202 -
YGHIPNGNRTYYLSRSQPP TREF_ECOLI 207 207 -
YGFIPNGARVFYLNRSQPP TREA_TENMO 203 203 -
YGFVPNGGRVYYLQRSQPP Q23176 195 195 -
HGFVPNGGRVYYLTRSQPP O44736 184 184 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LSRYRPEGAGVPPETEAD TREB_MAGGR 386 49 -
LSRYRSPGIGVPPETEAS TREB_EMENI 396 49 -
LSRYRPEGLGVPPETEAG TREB_NEUCR 393 49 -
LSCYHPEGKGIPPETEPT TREA_CANAL 563 54 -
LSCYHPDGIGIPPETEPG TREA_KLULA 414 51 -
LSRYHPNGLGIPPETESD TREA_YEAST 411 51 -
LSRYRPGGLGIPPETEAS TREA_SCHPO 390 49 -
LNRYHVPYGGPRPESYSK TREA_RABIT 275 49 -
LSCYHSDGIGIPPETEPD TREB_YEAST 440 51 -
LLRYYIPSAGPRPESYYE TREA_BOMMO 268 49 -
LNRYYVPYGGPRPESYSK TREA_HUMAN 275 49 -
LLRYYIPSAGPRPESYYE Q27453 268 49 -
LNRYWDDRDTPRPESWVE TREA_ECOLI 266 56 -
LYRFVVETSHPRPESYRE Q22195 245 47 -
LSRYYAMWNKPRPESSVF O22986 272 51 -
LNRYWDDRDTPRDESWLE TREF_ECOLI 281 55 -
LAQYNSNSGSPRPESYYE TREA_TENMO 271 49 -
VYQYKTASNVPRPESYRV Q23176 263 49 -
YYQYKAKLKVPRPESYRE O44736 254 51 -

Motif 3 width=18
Element Seqn Id St Int Rpt
FLHDRAVRESGHDTSYRL TREB_MAGGR 441 37 -
FMHDRAVRESGHDTSYRL TREB_EMENI 451 37 -
FMHDRAVRESGHDTTYRF TREB_NEUCR 448 37 -
FLHDRAVRESGHDTSYRL TREA_CANAL 619 38 -
FLHDRGVRESGHDTTYRF TREA_KLULA 469 37 -
FLHDRGVRESGHDTTYRF TREA_YEAST 466 37 -
FVHDRAVRESGHDTTYRL TREA_SCHPO 445 37 -
WAELKAGAESGWDFSSRW TREA_RABIT 309 16 -
FLHDRAVRESGHDTTYRF TREB_YEAST 495 37 -
YADLKSAAESGWDFSTRW TREA_BOMMO 303 17 -
WAELKAGAESGWDFSSRW TREA_HUMAN 309 16 -
YADLKSAAESGWDFSTRW Q27453 303 17 -
YRDLRSAAASGWDFSSRW TREA_ECOLI 300 16 -
WGDLAAAAESGRDFSSRF Q22195 280 17 -
HRDIATAAESGCDFSTRW O22986 307 17 -
YRDLRAGAASGWDYSSRW TREF_ECOLI 314 15 -
YMDLKSAAESGWDFSSRW TREA_TENMO 306 17 -
YQDLASAAESGWDFSTRW Q23176 298 17 -
WSEIASAAETGWDFSTRW O44736 289 17 -

Motif 4 width=18
Element Seqn Id St Int Rpt
LATVDLNTLLFKYETDIA TREB_MAGGR 465 6 -
LATVDLNSLLYKYEVDIA TREB_EMENI 475 6 -
LATIDLNSLLFKYETDIA TREB_NEUCR 472 6 -
LATVDLNSLLYKYENDIA TREA_CANAL 643 6 -
LATIDLNSLLYKYEVDIA TREA_KLULA 493 6 -
LATIDLNSLLYKYEIDIA TREA_YEAST 490 6 -
LATVDLNSLLYKYETDIS TREA_SCHPO 469 6 -
LVPVDLNAFLCQAEELLS TREA_RABIT 344 17 -
LATIDLNSLLYKYEKDIA TREB_YEAST 519 6 -
VIPVDLNAIFAGALQITA TREA_BOMMO 340 19 -
LVPVDLNAFLCQAEELMS TREA_HUMAN 344 17 -
VIPVDLNAIFAGALQITA Q27453 340 19 -
IVPVDLNSLMFKMEKILA TREA_ECOLI 332 14 -
LIPVDLNSIICGNMKTLS Q22195 316 18 -
VVPVDLNVFLLKMELDIA O22986 339 14 -
FIPIDLNAFLFKLESAIA TREF_ECOLI 346 14 -
IIPVDLNAFLCQAFQKLS TREA_TENMO 343 19 -
VLPVDLNGLLCWNMDIME Q23176 330 14 -
IIPADLNAFMCANARILA O44736 328 21 -

Motif 5 width=17
Element Seqn Id St Int Rpt
IDKYLWNEEAGMYFDYD TREB_MAGGR 526 43 -
MDTYLWDEEKGMYFDYD TREB_EMENI 536 43 -
IDKYLWNEEAGMYFDYN TREB_NEUCR 534 44 -
VDKYLWNEQDGIYYDYN TREA_CANAL 700 39 -
IDKYLWDEETGFYYDYN TREA_KLULA 545 34 -
ITKYMWDDESGFFFDYN TREA_YEAST 543 35 -
MEKYLWSEADSMWYDYN TREA_SCHPO 521 34 -
LTALLWDEDKGAWFDYD TREA_RABIT 387 25 -
INKYMWDEDSGFFFYYN TREB_YEAST 572 35 -
IEQALWDEEDGVWHDYD TREA_BOMMO 383 25 -
LNTVLWDEQTGAWFDYD TREA_HUMAN 387 25 -
IEQALWDEEDGVWHDYD Q27453 383 25 -
IEKYLWNDQQGWYADYD TREA_ECOLI 375 25 -
IRQVLWNEEHNCWFDFD Q22195 359 25 -
FQTVFWNEKAGQWLDYW O22986 382 25 -
VNRYLWDDENGIYRDYD TREF_ECOLI 389 25 -
IEMVHYNRDDGIWYDWD TREA_TENMO 386 25 -
VQNVFYNRTDGTWYDYN Q23176 373 25 -
MRELHWNETDGIWYDYD O44736 371 25 -

Motif 6 width=17
Element Seqn Id St Int Rpt
GLDRPNRQWDYPYGWAP TREB_MAGGR 599 56 -
GLNRPTRQWDYPYGWAP TREB_EMENI 609 56 -
GLERPNRQWDYPYGWAP TREB_NEUCR 607 56 -
GLTRPSRQWDYPFGWAP TREA_CANAL 773 56 -
SLDRPTRQWDYPFGWAP TREA_KLULA 618 56 -
SISRPIRQWDYPFGWAP TREA_YEAST 616 56 -
GLDNPSRQWDYPNGWSP TREA_SCHPO 594 56 -
SLQNTGQQWDFPNAWAP TREA_RABIT 454 50 -
SIDRPIRQWDYPFGWAP TREB_YEAST 645 56 -
SLVNSGEQWDFPNAWPP TREA_BOMMO 453 53 -
SLQKTGQQWDFPNAWAP TREA_HUMAN 454 50 -
SLINSGEQWDFPNAWPP Q27453 453 53 -
TSVKSGQQWDAPNGWAP TREA_ECOLI 439 47 -
SLVNSGEQWDFPNSWPP Q22195 426 50 -
SLTNSGQQWDSPNGWAP O22986 451 52 -
SEYETGEQWDKPNGWAP TREF_ECOLI 452 46 -
SLSHTGEQWDYPNAWPP TREA_TENMO 456 53 -
MSQESDQQWDFPNGWSP Q23176 443 53 -
LAMSSTQQWDKENAWPP O44736 440 52 -

Motif 7 width=14
Element Seqn Id St Int Rpt
EGFGWVNASYIYGL TREB_MAGGR 687 71 -
EGFGWVNASYVYGL TREB_EMENI 697 71 -
EGFGWVNASYVYGL TREB_NEUCR 695 71 -
EGFGWVNASYVFGL TREA_CANAL 861 71 -
EGFGWVNSSYLLGM TREA_KLULA 706 71 -
EGFGWVNASYILGL TREA_YEAST 704 71 -
EGFGWVNASYEVGL TREA_SCHPO 682 71 -
EGFGWTNGVALMLL TREA_RABIT 533 62 -
EGFGWVNTSYLLGL TREB_YEAST 733 71 -
DGFGWSNGVVLEFL TREA_BOMMO 533 63 -
EGFGWDEGVVLMLL TREA_HUMAN 534 63 -
DGFGWSNGVVLEFL Q27453 533 63 -
DGFGWTNGVTLKML TREA_ECOLI 516 60 -
EGFGWTNGVILDFL Q22195 505 62 -
TGFGWSNGVILAFL O22986 531 63 -
DGFGWTNGVVRRLI TREF_ECOLI 530 61 -
SGFGWTNGVVLEFI TREA_TENMO 536 63 -
DGFGWSNGAILDLL Q23176 524 64 -
TGFGWTNGVILDLL O44736 523 66 -