| Literature References | 1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
5. HAHN, M., KEITEL, T. AND HEINEMANN, U.
Crystal and molecular structure at 0.16nm resolution of the hybrid Bacillus
endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase H(A16-M).
EUR.J.BIOCHEMISTRY 232(3) 849-858 (1995).
6. JUNCOSA, M., PONS, J., DOT, T., QUEROL, E., PLANAS, A.
Identification of active-site carboxylic residues in Bacillus licheniformis
1,3-1,4-beta-d-glucan 4-glucanohydrolase by site-directed mutagenesis.
J.BIOL.CHEM. 269 14530-14535 (1994).
|
| Documentation | O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt).
Family 16 mainly encompasses bacterial beta-glucanases. These enzymes
hydrolyse 1,4-beta-D-glycosidic linkages in beta-D-glucans containing
1,3- and 1,4-bonds.
The 3D structure of a hybrid endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase
from Bacillus has been refined to 1.6A [5]. The protein belongs to the
mainly-beta class, with 16 strands and 3 helices; the fold reveals a
compact jellyroll-type beta-sheet structure that comprises two major
anti-parallel beta-sheets and a 3-stranded minor mixed sheet. Amino acid
residues involved in catalysis (two closely-clustered conserved glutamates
[6]) and substrate binding are located inside a deep channel spanning the
surface of the protein [5].
GLHYDRLASE16 is an 8-element fingerprint that provides a signature for
family 16 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from short conserved regions
spanning the N-terminal half of the alignment - motif 1 spans beta-strands
6 and 7; motif 2 includes strand 8; motif 3 spans strand 9 and includes the
region encoded by PROSITE pattern GLYCOSYL_HYDROL_F16 (PS01034), which
contains the catalytic cluster of acidic residues; motif 4 includes strand
10; motif 5 spans helix 2 and strand 12; motif 6 spans strands 13 and 14;
motif 7 spans strand 15 and helix 3; and motif 7 includes strand 16. Two
iterations on OWL29.3 were required to reach convergence, at which point
a true set comprising 25 sequences was identified. Several partial matches
were also found, all of which are related sequences that fail to make
significant matches with one or more motifs.
An update on SPTR37_9f identified a true set of 12 sequences, and 11
partial matches.
|
| Sequence Titles | GUB_BACAM BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - BACILLUS AMYLOLIQUEFACIENS.
GUB_BACBR BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - BACILLUS BREVIS.
GUB_BACLI BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - BACILLUS LICHENIFORMIS.
GUB_BACSU BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - BACILLUS SUBTILIS.
GUB_CLOTM BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) (LAMINARINASE) - CLOSTRIDIUM THERMOCELLUM.
GUB_PAEMA BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - PAENIBACILLUS MACERANS (BACILLUS MACERANS).
GUB_PAEPO BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - PAENIBACILLUS POLYMYXA (BACILLUS POLYMYXA).
O07856 BETA-(1,3-1,4)-GLUCANASE PRECURSOR - STREPTOCOCCUS BOVIS.
O14412 BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - ORPINOMYCES SP.
Q45648 LICHENASE PRECURSOR - BACILLUS SP.
Q45691 ENDO-BETA-1,3-1,4-GLUCANASE - BACILLUS SUBTILIS.
XYND_RUMFL XYLANASE/BETA-GLUCANASE PRECURSOR [INCLUDES: ENDO-1,4-BETA-XYLANASE (EC 3.2.1.8) (XYLANASE); (ENDO-BETA-1,3-1,4 GLUCANASE) (EC 3.2.1.73) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE)] - RUMINOCOCCUS FLAVEFACIENS.
EXOK_RHIME SUCCINOGLYCAN BIOSYNTHESIS PROTEIN EXOK - RHIZOBIUM MELILOTI.
CGKA_ALTCA KAPPA-CARRAGEENASE PRECURSOR (EC 3.2.1.83) - ALTEROMONAS CARRAGEENOVORA.
GUB_FIBSU BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (MIXED LINKAGE BETA- GLUCANASE) (LICHENASE) - FIBROBACTER SUCCINOGENES (BACTEROIDES SUCCINOGENES).
O68641 BETA-1,3-GLUCANASE II - OERSKOVIA XANTHINEOLYTICA.
O80803 T8F5.9 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
P93669 XYLOGLUCAN ENDOTRANSGLYCOSYLASE-LIKE PROTEIN - HORDEUM VULGARE (BARLEY).
P93670 XYLOGLUCAN ENDOTRANSGLYCOSYLASE (XET) - HORDEUM VULGARE (BARLEY).
Q39099 ENDO-XYLOGLUCAN TRANSFERASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q51333 BETA-1,3-GLUCANASE IIA - OERSKOVIA XANTHINEOLYTICA.
Q59328 ENDO-1,3(4)-BETA-GLUCANASE (EC 3.2.1.6) (ENDO-1,4-BETA-GLUCANASE) (ENDO-1,3-BETA-GLUCANASE) (LAMINARINASE) - CLOSTRIDIUM THERMOCELLUM.
YG46_YEAST HYPOTHETICAL 52.8 KD PROTEIN IN BUB1-HIP1 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
|