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PR00737

Identifier
GLHYDRLASE16  [View Relations]  [View Alignment]  
Accession
PR00737
No. of Motifs
8
Creation Date
05-JUN-1997  (UPDATE 21-JUN-1999)
Title
Glycosyl hydrolase family 16 signature
Database References

PROSITE; PS01034 GLYCOSYL_HYDROL_F16
BLOCKS; BL01034
PFAM; PF00722 glycosyl_hydro9
INTERPRO; IPR000757
PDB; 1AYH
SCOP; 1CPM
CATH; 1AYH
Literature References
1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
 
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
5. HAHN, M., KEITEL, T. AND HEINEMANN, U.
Crystal and molecular structure at 0.16nm resolution of the hybrid Bacillus
endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase H(A16-M).
EUR.J.BIOCHEMISTRY 232(3) 849-858 (1995).
 
6. JUNCOSA, M., PONS, J., DOT, T., QUEROL, E., PLANAS, A.
Identification of active-site carboxylic residues in Bacillus licheniformis
1,3-1,4-beta-d-glucan 4-glucanohydrolase by site-directed mutagenesis.
J.BIOL.CHEM. 269 14530-14535 (1994).

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt).
 
Family 16 mainly encompasses bacterial beta-glucanases. These enzymes
hydrolyse 1,4-beta-D-glycosidic linkages in beta-D-glucans containing
1,3- and 1,4-bonds.
 
The 3D structure of a hybrid endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase
from Bacillus has been refined to 1.6A [5]. The protein belongs to the
mainly-beta class, with 16 strands and 3 helices; the fold reveals a
compact jellyroll-type beta-sheet structure that comprises two major
anti-parallel beta-sheets and a 3-stranded minor mixed sheet. Amino acid
residues involved in catalysis (two closely-clustered conserved glutamates
[6]) and substrate binding are located inside a deep channel spanning the
surface of the protein [5]. 
 
GLHYDRLASE16 is an 8-element fingerprint that provides a signature for
family 16 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from short conserved regions 
spanning the N-terminal half of the alignment - motif 1 spans beta-strands
6 and 7; motif 2 includes strand 8; motif 3 spans strand 9 and includes the
region encoded by PROSITE pattern GLYCOSYL_HYDROL_F16 (PS01034), which
contains the catalytic cluster of acidic residues; motif 4 includes strand
10; motif 5 spans helix 2 and strand 12; motif 6 spans strands 13 and 14;
motif 7 spans strand 15 and helix 3; and motif 7 includes strand 16. Two
iterations on OWL29.3 were required to reach convergence, at which point
a true set comprising 25 sequences was identified. Several partial matches
were also found, all of which are related sequences that fail to make
significant matches with one or more motifs.
 
An update on SPTR37_9f identified a true set of 12 sequences, and 11
partial matches.
Summary Information
  12 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
10 codes involving 2 elements
Composite Feature Index
81212121212121212
700000000
600000000
511110100
400000000
300000000
241515400
12345678
True Positives
GUB_BACAM     GUB_BACBR     GUB_BACLI     GUB_BACSU     
GUB_CLOTM GUB_PAEMA GUB_PAEPO O07856
O14412 Q45648 Q45691 XYND_RUMFL
True Positive Partials
Codes involving 5 elements
EXOK_RHIME
Codes involving 2 elements
CGKA_ALTCA GUB_FIBSU O68641 O80803
P93669 P93670 Q39099 Q51333
Q59328 YG46_YEAST
Sequence Titles
GUB_BACAM   BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - BACILLUS AMYLOLIQUEFACIENS. 
GUB_BACBR BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - BACILLUS BREVIS.
GUB_BACLI BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - BACILLUS LICHENIFORMIS.
GUB_BACSU BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - BACILLUS SUBTILIS.
GUB_CLOTM BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) (LAMINARINASE) - CLOSTRIDIUM THERMOCELLUM.
GUB_PAEMA BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - PAENIBACILLUS MACERANS (BACILLUS MACERANS).
GUB_PAEPO BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - PAENIBACILLUS POLYMYXA (BACILLUS POLYMYXA).
O07856 BETA-(1,3-1,4)-GLUCANASE PRECURSOR - STREPTOCOCCUS BOVIS.
O14412 BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE) - ORPINOMYCES SP.
Q45648 LICHENASE PRECURSOR - BACILLUS SP.
Q45691 ENDO-BETA-1,3-1,4-GLUCANASE - BACILLUS SUBTILIS.
XYND_RUMFL XYLANASE/BETA-GLUCANASE PRECURSOR [INCLUDES: ENDO-1,4-BETA-XYLANASE (EC 3.2.1.8) (XYLANASE); (ENDO-BETA-1,3-1,4 GLUCANASE) (EC 3.2.1.73) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (LICHENASE)] - RUMINOCOCCUS FLAVEFACIENS.

EXOK_RHIME SUCCINOGLYCAN BIOSYNTHESIS PROTEIN EXOK - RHIZOBIUM MELILOTI.

CGKA_ALTCA KAPPA-CARRAGEENASE PRECURSOR (EC 3.2.1.83) - ALTEROMONAS CARRAGEENOVORA.
GUB_FIBSU BETA-GLUCANASE PRECURSOR (EC 3.2.1.73) (ENDO-BETA-1,3-1,4 GLUCANASE) (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) (MIXED LINKAGE BETA- GLUCANASE) (LICHENASE) - FIBROBACTER SUCCINOGENES (BACTEROIDES SUCCINOGENES).
O68641 BETA-1,3-GLUCANASE II - OERSKOVIA XANTHINEOLYTICA.
O80803 T8F5.9 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
P93669 XYLOGLUCAN ENDOTRANSGLYCOSYLASE-LIKE PROTEIN - HORDEUM VULGARE (BARLEY).
P93670 XYLOGLUCAN ENDOTRANSGLYCOSYLASE (XET) - HORDEUM VULGARE (BARLEY).
Q39099 ENDO-XYLOGLUCAN TRANSFERASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q51333 BETA-1,3-GLUCANASE IIA - OERSKOVIA XANTHINEOLYTICA.
Q59328 ENDO-1,3(4)-BETA-GLUCANASE (EC 3.2.1.6) (ENDO-1,4-BETA-GLUCANASE) (ENDO-1,3-BETA-GLUCANASE) (LAMINARINASE) - CLOSTRIDIUM THERMOCELLUM.
YG46_YEAST HYPOTHETICAL 52.8 KD PROTEIN IN BUB1-HIP1 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Scan History
OWL29_3    2  100  NSINGLE    
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
AEYRSTNIYGYGLYEVSMK GUB_BACMA 85 85 -
GENRSVQTYGYGLYEVNMK GUB_BACLI 91 91 -
GENRSVQTYGYGLYEVRMK GUB_BACAM 87 87 -
GENRSVQTYGYGLYEVRMK GUB_BACSU 90 90 -
GEYRTKSFFGYGYYEVRMK GUB_CLOTM 93 93 -
GELRTNDFYHYGLFEVSMK GUB_BACBR 97 97 -
GEYRSTNNYGYGLYEVSMK GUB_BACPO 86 86 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PAKNTGIVSSFFTYTGP GUB_BACSU 109 0 -
PAKNTGIVSSFFTYTGP GUB_BACAM 106 0 -
PAKNTGIVSSFFTYTGP GUB_BACMA 104 0 -
PAKNTGIVSSFFTYTGP GUB_BACPO 105 0 -
AAKNVGIVSSFFTYTGP GUB_CLOTM 112 0 -
PAKVEGTVSSFFTYTGE GUB_BACBR 116 0 -
PAKNVGIVSSFFTYTGP GUB_BACLI 110 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
QWDEIDIEFLGKDTTK GUB_BACPO 126 4 -
QWDEIDIEFLGKDTTK GUB_BACMA 125 4 -
PWDEIDIEFLGKDTTK GUB_BACLI 131 4 -
PWDEIDIEFLGKDTTR GUB_BACBR 139 6 -
PWDEIDIEFLGKDTTK GUB_BACAM 127 4 -
PWDEIDIEFLGKDTTK GUB_BACSU 130 4 -
PWDEIDIEFLGKDTTK GUB_CLOTM 133 4 -

Motif 4 width=14
Element Seqn Id St Int Rpt
VQFNYYTNGVGGHE GUB_BACPO 142 0 -
VQFNYYTNGVGGHE GUB_BACMA 141 0 -
VQFNYYTNGAGNHE GUB_BACSU 146 0 -
VQFNYYTNGAGNHE GUB_BACAM 143 0 -
VQFNYYTNGVGNHE GUB_BACLI 147 0 -
IQFNYFTNGVGGNE GUB_BACBR 155 0 -
VQFNWYKNGVGGNE GUB_CLOTM 149 0 -

Motif 5 width=18
Element Seqn Id St Int Rpt
LGFDAANAYHTYAFDWQP GUB_BACSU 164 4 -
LGFDASTSFHTYAFDWQP GUB_BACPO 160 4 -
LGFDASKGFHTYAFDWQP GUB_BACMA 159 4 -
LGFDASQDFHTYGFEWRP GUB_CLOTM 167 4 -
LGFDASESFNTYAFEWRE GUB_BACBR 173 4 -
LGFDAANSYHTYAFDWQP GUB_BACLI 165 4 -
LGFDAANAYHTYAFDWQP GUB_BACAM 161 4 -

Motif 6 width=18
Element Seqn Id St Int Rpt
IKWYVDGVLKHTATTNIP GUB_BACPO 180 2 -
IKWYVDGQLKHTATNQIP GUB_BACSU 184 2 -
IKWYVDGQLKHTATTQIP GUB_BACAM 181 2 -
IKWYVDGQLKHTATTQIP GUB_BACLI 185 2 -
ITWYVNGEAVHTATENIP GUB_BACBR 193 2 -
IDFYVDGKKVYRGTRNIP GUB_CLOTM 187 2 -
IKWYVDGVLKHTATANIP GUB_BACMA 179 2 -

Motif 7 width=23
Element Seqn Id St Int Rpt
PGKIMMNLWPGIGVDEWLGRYDG GUB_CLOTM 207 2 -
PGKIMMNLWNGTGVDSWLGSYNG GUB_BACPO 200 2 -
PGKIMMNLWNGTGVDDWLGSYNG GUB_BACMA 199 2 -
PGKIMMNLWNGTGVDEWLGSYNG GUB_BACSU 204 2 -
PGKIMMNLWNGTGVDDWLGSYNG GUB_BACAM 201 2 -
PGKIMMNLWNGAGVDEWLGSYNG GUB_BACLI 205 2 -
PQKIMMNLWPGVGVDGWTGVFDG GUB_BACBR 213 2 -

Motif 8 width=13
Element Seqn Id St Int Rpt
NPLYAEYDWVKYT GUB_BACPO 224 1 -
NPLYAEYDWVKYT GUB_BACMA 223 1 -
TPLQAEYEYVKYY GUB_CLOTM 231 1 -
TPVYSYYDWVRYT GUB_BACBR 238 2 -
TPLSRSLHWVRYT GUB_BACLI 229 1 -
NPIYAHYDWMRYR GUB_BACAM 225 1 -
NPLYAHYDWVRYT GUB_BACSU 228 1 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
GENRSVQTYGYGLYEVRMK GUB_BACSU 90 90 -
GEYRSTNNYGYGLYEVSMK GUB_PAEPO 86 86 -
AEYRSTNIYGYGLYEVSMK GUB_PAEMA 85 85 -
GENRSVQTYGYGLYEVRMK Q45691 87 87 -
GENRSVQTYGYGLYEVRMK GUB_BACAM 87 87 -
GENRSVQTYGYGLYEVNMK GUB_BACLI 91 91 -
GELRTNDFYHYGLFEVSMK Q45648 99 99 -
GELRTNDFYHYGLFEVSMK GUB_BACBR 97 97 -
GEYRTKNYYGYGMFQVNMK O14412 91 91 -
GEYRTKSFFGYGYYEVRMK GUB_CLOTM 93 93 -
GEWRSKERFGYGLFQVNMK O07856 84 84 -
GEFRTNNFYHYGYYECSMQ XYND_RUMFL 637 637 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PAKNTGIVSSFFTYTGP GUB_BACSU 109 0 -
PAKNTGIVSSFFTYTGP GUB_PAEPO 105 0 -
PAKNTGIVSSFFTYTGP GUB_PAEMA 104 0 -
PAKNTGIVSSFFTYTGP Q45691 106 0 -
PAKNTGIVSSFFTYTGP GUB_BACAM 106 0 -
PAKNVGIVSSFFTYTGP GUB_BACLI 110 0 -
PAKSTGTVSSFFTYTGP Q45648 118 0 -
PAKVEGTVSSFFTYTGE GUB_BACBR 116 0 -
PIKNPGVVSSFFTYTGP O14412 110 0 -
AAKNVGIVSSFFTYTGP GUB_CLOTM 112 0 -
PIKNPGVVSSFFTYTGP O07856 103 0 -
AMKNDGVVSSFFTYTGP XYND_RUMFL 656 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
PWDEIDIEFLGKDTTK GUB_BACSU 130 4 -
QWDEIDIEFLGKDTTK GUB_PAEPO 126 4 -
QWDEIDIEFLGKDTTK GUB_PAEMA 125 4 -
PWDEIDIEFLGKDTTK Q45691 127 4 -
PWDEIDIEFLGKDTTK GUB_BACAM 127 4 -
PWDEIDIEFLGKDTTK GUB_BACLI 131 4 -
PWDEIDIEFLGKDTTK Q45648 141 6 -
PWDEIDIEFLGKDTTR GUB_BACBR 139 6 -
KWDEIDIEFLGYDTTK O14412 131 4 -
PWDEIDIEFLGKDTTK GUB_CLOTM 133 4 -
KWDEIDIEFLGKDTTK O07856 124 4 -
PWDEIDIEILGKNTTQ XYND_RUMFL 677 4 -

Motif 4 width=14
Element Seqn Id St Int Rpt
VQFNYYTNGAGNHE GUB_BACSU 146 0 -
VQFNYYTNGVGGHE GUB_PAEPO 142 0 -
VQFNYYTNGVGGHE GUB_PAEMA 141 0 -
VQFNYYTNGAENHE Q45691 143 0 -
VQFNYYTNGAGNHE GUB_BACAM 143 0 -
VQFNYYTNGVGNHE GUB_BACLI 147 0 -
IQFNYFTNGVGGNE Q45648 157 0 -
IQFNYFTNGVGGNE GUB_BACBR 155 0 -
VQFNYYTNGQGHHE O14412 147 0 -
VQFNWYKNGVGGNE GUB_CLOTM 149 0 -
VQFNYYTSGQGNHE O07856 140 0 -
VQFNYYTNGQGKHE XYND_RUMFL 693 0 -

Motif 5 width=18
Element Seqn Id St Int Rpt
LGFDAANAYHTYAFDWQP GUB_BACSU 164 4 -
LGFDASTSFHTYAFDWQP GUB_PAEPO 160 4 -
LGFDASKGFHTYAFDWQP GUB_PAEMA 159 4 -
LGFDAANAYHTYAFDWQP Q45691 161 4 -
LGFDAANAYHTYAFDWQP GUB_BACAM 161 4 -
LGFDAANSYHTYAFDWQP GUB_BACLI 165 4 -
LGFDAADDFNTYAFEWRP Q45648 175 4 -
LGFDASESFNTYAFEWRE GUB_BACBR 173 4 -
LGFDASQGFHTYGFFWAR O14412 165 4 -
LGFDASQDFHTYGFEWRP GUB_CLOTM 167 4 -
LGFDASQGFHTYGFDWQA O07856 158 4 -
LGFDSSEAYHTYGFDWQP XYND_RUMFL 711 4 -

Motif 6 width=18
Element Seqn Id St Int Rpt
IKWYVDGQLKHTATNQIP GUB_BACSU 184 2 -
IKWYVDGVLKHTATTNIP GUB_PAEPO 180 2 -
IKWYVDGVLKHTATANIP GUB_PAEMA 179 2 -
IKWYVDWQLKHTATTQIP Q45691 181 2 -
IKWYVDGQLKHTATTQIP GUB_BACAM 181 2 -
IKWYVDGQLKHTATTQIP GUB_BACLI 185 2 -
IRWFVNGELVHTATENIP Q45648 195 2 -
ITWYVNGEAVHTATENIP GUB_BACBR 193 2 -
ITWYVDGTAVYTAYDNIP O14412 185 2 -
IDFYVDGKKVYRGTRNIP GUB_CLOTM 187 2 -
ITWYVDGRAVYTAYNNIP O07856 178 2 -
IAWYVDGREVYRATQDIP XYND_RUMFL 731 2 -

Motif 7 width=23
Element Seqn Id St Int Rpt
PGKIMMNLWNGTGVDEWLGSYNG GUB_BACSU 204 2 -
PGKIMMNLWNGTGVDSWLGSYNG GUB_PAEPO 200 2 -
PGKIMMNLWNGTGVDDWLGSYNG GUB_PAEMA 199 2 -
PGKIMMNLWNGTGVSDWLGSYNG Q45691 201 2 -
PGKIMMNLWNGTGVDDWLGSYNG GUB_BACAM 201 2 -
PGKIMMNLWNGAGVDEWLGSYNG GUB_BACLI 205 2 -
PQKIMMNLWPGIGVDGWTGRFNG Q45648 215 2 -
PQKIMMNLWPGVGVDGWTGVFDG GUB_BACBR 213 2 -
PGKIMMNAWNGIGVDDWLRPFNG O14412 205 2 -
PGKIMMNLWPGIGVDEWLGRYDG GUB_CLOTM 207 2 -
PGKIMMNAWPGTHEVDSWLGAYN O07856 198 2 -
PGKIMMNAWPGLTVDDWLKAFNG XYND_RUMFL 751 2 -

Motif 8 width=13
Element Seqn Id St Int Rpt
NPLYAHYDWVRYT GUB_BACSU 228 1 -
NPLYAEYDWVKYT GUB_PAEPO 224 1 -
NPLYAEYDWVKYT GUB_PAEMA 223 1 -
NPLYAHYDWVRYT Q45691 225 1 -
NPIYAHYDWMRYR GUB_BACAM 225 1 -
TPLSRSLHWVRYT GUB_BACLI 229 1 -
TPVVTQYDWVKYT Q45648 240 2 -
TPVYSYYDWVRYT GUB_BACBR 238 2 -
TNISAYYDWVSYD O14412 229 1 -
TPLQAEYEYVKYY GUB_CLOTM 231 1 -
TPLYAYYDWISYD O07856 223 2 -
TPLTAHYQWVTYN XYND_RUMFL 775 1 -