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PR00733

Identifier
GLHYDRLASE6  [View Relations]  [View Alignment]  
Accession
PR00733
No. of Motifs
8
Creation Date
04-JUN-1997  (UPDATE 21-JUN-1999)
Title
Glycosyl hydrolase family 6 signature
Database References

PROSITE; PS00655 GLYCOSYL_HYDROL_F6_1; PS00656 GLYCOSYL_HYDROL_F6_2
BLOCKS; BL00655
PFAM; PF01341 Glycosyl_hydr21
INTERPRO; IPR001524
PDB; 3CBH
SCOP; 3CBH
Literature References
1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
 
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
5. ROUVINEN, J., BERGFORS, T., TEERI, T., KNOWLES, J.K.C. AND JONES, T.A.
3-Dimensional structure of cellobiohydrolase-II from Trichoderma reesei.
SCIENCE 249 380-386 (1990). 

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 6 includes endoglucanases and cellobiohydrolases,
which are essential enzymes for microbial degradation of cellulose and
xylans. 
 
The 3D structure of the enzymatic core of cellobiohydrolase II (CBHII) from
the fungus Trichoderma reesei reveals an alpha-beta protein with a fold
similar to the ubiquitous barrel topology first seen in triose phosphate
isomerase [5]. The active site of CBHII is located at the C-terminal end of
a parallel beta barrel, in an enclosed tunnel through which the cellulose
threads. Two aspartic acid residues, located in the center of the tunnel
are the probable catalytic residues [5].
 
GLHYDRLASE6 is an 8-element fingerprint that provides a signature for 
family 6 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions 
within the catalytic domain - motif 2 includes part of the region encoded
by PROSITE pattern GLYCOSYL_HYDROL_F6_1 (PS00655), which contains a
conserved aspartic acid residue that may be involved in the catalytic
mechanism [5]. Two iterations on OWL29.3 were required to reach convergence,
at which point a true set comprising 16 sequences was identified. Several
partial matches were also found, all of which are family members that fail
to make significant matches with one or more motifs.
 
An update on SPTR37_9f identified a true set of 18 sequences, and 1
partial match.
Summary Information
18 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
81818181818181818
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
GUN1_STRHA    GUN1_STRSQ    GUN2_THEFU    GUNA_CELFI    
GUNA_MICBI GUNB_FUSOX GUX2_TRIRE GUX3_AGABI
GUXA_CELFI O53607 O86730 P78720
P78721 Q02321 Q12646 Q50901
Q53488 Q60029
Sequence Titles
GUN1_STRHA  ENDOGLUCANASE 1 PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCANASE 1) (CELLULASE I) (CMCASE I) (CEL1) - STREPTOMYCES HALSTEDII. 
GUN1_STRSQ ENDOGLUCANASE 1 PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCANASE) (CELLULASE) (CARBOXYMETHYL CELLULASE) (CMCASE I) - STREPTOMYCES SP. (STRAIN KSM-9).
GUN2_THEFU ENDOGLUCANASE E-2 PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCANASE E-2) (CELLULASE E-2) (CELLULASE E2) - THERMOMONOSPORA FUSCA.
GUNA_CELFI ENDOGLUCANASE A PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCANASE) (CELLULASE) - CELLULOMONAS FIMI.
GUNA_MICBI ENDOGLUCANASE A PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA-GLUCANASE) (CELLULASE) - MICROBISPORA BISPORA.
GUNB_FUSOX PUTATIVE ENDOGLUCANASE TYPE B PRECURSOR (EC 3.2.1.4) (ENDO-1,4-BETA- GLUCANASE) (CELLULASE) - FUSARIUM OXYSPORUM.
GUX2_TRIRE EXOGLUCANASE II PRECURSOR (EC 3.2.1.91) (EXOCELLOBIOHYDROLASE II) (CBHII) (1,4-BETA-CELLOBIOHYDROLASE) - TRICHODERMA REESEI (HYPOCREA JECORINA).
GUX3_AGABI EXOGLUCANASE 3 PRECURSOR (EC 3.2.1.91) (EXOCELLOBIOHYDROLASE 3) (1,4-BETA-CELLOBIOHYDROLASE 3) - AGARICUS BISPORUS (COMMON MUSHROOM).
GUXA_CELFI EXOGLUCANASE A PRECURSOR (EC 3.2.1.91) (EXOCELLOBIOHYDROLASE A) (1,4-BETA-CELLOBIOHYDROLASE A) (CBP95) - CELLULOMONAS FIMI.
O53607 PUTATIVE CELLULASE - MYCOBACTERIUM TUBERCULOSIS.
O86730 PUTATIVE SECRETED CELLULASE - STREPTOMYCES COELICOLOR.
P78720 CELLULASE A (EC 3.2.1.4) (ENDOGLUCANASE) (ENDO-1,4-BETA-GLUCANASE) (CARBOXYMETHYL CELLULASE) - ORPINOMYCES SP. PC-2.
P78721 CELLULASE C (EC 3.2.1.4) (ENDOGLUCANASE) (ENDO-1,4-BETA-GLUCANASE) (CARBOXYMETHYL CELLULASE) - ORPINOMYCES SP. PC-2.
Q02321 EXOCELLOBIOHYDROLASE - PHANEROCHAETE CHRYSOSPORIUM.
Q12646 CELLOBIOHYDROLASE PRECURSOR (EC 3.2.1.91) (CELLULOSE 1,4-BETA-CELLOBIOSIDASE) (EXOGLUCANASE) (EXOCELLOBIOHYDROLASE) (1,4-BETA-CELLOBIOHYDROLASE) - NEOCALLIMASTIX PATRICIARUM (RUMEN FUNGUS).
Q50901 BETA-1,4-GLYCANASE - MYXOCOCCUS XANTHUS.
Q53488 ENDO-BETA-1,4-GLUCANASE - MICROMONOSPORA CELLULOLYTICUM.
Q60029 BETA-1,4-EXOCELLULASE PRECURSOR (EC 3.2.1.91) (CELLULOSE 1,4-BETA-CELLOBIOSIDASE) (EXOGLUCANASE) (EXOCELLOBIOHYDROLASE) (1,4-BETA-CELLOBIOHYDROLASE) - THERMOMONOSPORA FUSCA.
Scan History
OWL29_3    3  100  NSINGLE    
SPTR37_9f 3 50 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
DPRTPVIRDRIASVPQGTWF GUN2_THEFU 54 54 -
NPSLATKAASVAKIPTFVWF GUX3_AGABI 116 116 -
DPRTPVIRDRIAAVPTGRWF GUNA_MICBI 57 57 -
SGTDKALLEKIALTPQAYWV GUNA_CELFI 190 190 -
SGAKATAAAKVADVPSFQWM GUNB_FUSOX 132 132 -
DGRAAAINASIANTPMARWF GUN1_STRHA 55 55 -
DHRAPLIAERIGSQPQAVWF GUN1_STRSQ 97 97 -

Motif 2 width=14
Element Seqn Id St Int Rpt
KIPILVVYNAPGRD GUN2_THEFU 97 23 -
QLVQIVVYDLPDRD GUX3_AGABI 156 20 -
KIPIMVVYAMPNRD GUNA_MICBI 100 23 -
KTPMLVVYAIPGRD GUNA_CELFI 234 24 -
YAGQFVVYDLPNRD GUNB_FUSOX 177 25 -
KLPILVAYNIYNRD GUN1_STRHA 97 22 -
QLPVVVPYMIPFRD GUN1_STRSQ 141 24 -

Motif 3 width=16
Element Seqn Id St Int Rpt
YRSWIDEFAAGLKNRP GUN2_THEFU 125 14 -
YKNYVDQIAAQIKQFP GUX3_AGABI 189 19 -
YRAWIDEIAAGLRNRP GUNA_MICBI 128 14 -
YARWVDTVAQGIKGNP GUNA_CELFI 261 13 -
YKAYIAKIKGILQNYS GUNB_FUSOX 210 19 -
YADWIARFAGGIAARP GUN1_STRHA 126 15 -
YAEWSGLFAAGLGSEP GUN1_STRSQ 169 14 -

Motif 4 width=14
Element Seqn Id St Int Rpt
QARIYFDAGHSAWH GUN2_THEFU 181 40 -
GVTMYIDAGHAGWL GUX3_AGABI 251 46 -
QAKVYFDAGHDAWV GUNA_MICBI 185 41 -
GARVYIDAGHAKWL GUNA_CELFI 312 35 -
NVSMYLDAGHGGWL GUNB_FUSOX 272 46 -
NTWVYMDAGNPRWA GUN1_STRHA 182 40 -
EARVYYDVGHSAWH GUN1_STRSQ 225 40 -

Motif 5 width=11
Element Seqn Id St Int Rpt
AHGIATNTSNY GUN2_THEFU 212 17 -
LRGIATNVANF GUX3_AGABI 290 25 -
ADGIALNVSNY GUNA_MICBI 216 17 -
AVGFALNTSNY GUNA_CELFI 342 16 -
VRGLVTNVSNY GUNB_FUSOX 311 25 -
AHGFSLNVSNY GUN1_STRHA 212 16 -
GAGIATNISNY GUN1_STRSQ 256 17 -

Motif 6 width=14
Element Seqn Id St Int Rpt
LRAVIDTSRNGNGP GUN2_THEFU 244 21 -
AHFIVDQGRSGVQN GUX3_AGABI 338 37 -
LRAVIDTSRNGNGP GUNA_MICBI 248 21 -
KKFVIDTSRNGNGS GUNA_CELFI 372 19 -
VKFIVDQGRSGKQP GUNB_FUSOX 360 38 -
KPFVVDTSRNGNGS GUN1_STRHA 247 24 -
LGAVVDTSRNGNGP GUN1_STRSQ 287 20 -

Motif 7 width=21
Element Seqn Id St Int Rpt
GNEWCDPSGRAIGTPSTTNTG GUN2_THEFU 259 1 -
WGDWCNVKGAGFGQRPTTNTG GUX3_AGABI 356 4 -
GSEWCDPPGRATGTWSTTDTG GUNA_MICBI 263 1 -
NGEWCNPRGRALGERPVAVND GUNA_CELFI 386 0 -
QGDWCNAKGTGFGLRPSTNTG GUNB_FUSOX 379 5 -
NGEWCNPSGRRIGTPTRTGGG GUN1_STRHA 261 0 -
GSEWCDPPGRLVGNNPTVNPG GUN1_STRSQ 302 1 -

Motif 8 width=15
Element Seqn Id St Int Rpt
IDAFLWIKLPGEADG GUN2_THEFU 283 3 -
IDAIVWVKPGGECDG GUX3_AGABI 380 3 -
IDAFLWIKPPGEADG GUNA_MICBI 287 3 -
LDALLWVKLPGESDG GUNA_CELFI 410 3 -
ADAFVWVKPGGESDG GUNB_FUSOX 403 3 -
AEMLLWIKTPGESDG GUN1_STRHA 282 0 -
VDAFLWIKLPGELDG GUN1_STRSQ 326 3 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
DPRTPVIRDRIASVPQGTWF GUN2_THEFU 54 54 -
SGSLQEKAKKVKYVPTAAWL P78721 155 155 -
NPSLATKAASVAKIPTFVWF GUX3_AGABI 116 116 -
DPRTPVIRDRIAAVPTGRWF GUNA_MICBI 57 57 -
NGDLKAKAEKVKYVPTAVWL P78720 156 156 -
SYDLQQKAQKVKNVPTAVWL Q12646 131 131 -
SGTDKALLEKIALTPQAYWV GUNA_CELFI 190 190 -
DPTLSSKAASVANIPTFTWL Q02321 132 132 -
SGAKATAAAKVADVPSFQWM GUNB_FUSOX 132 132 -
DGRAAAINASIANTPMARWF GUN1_STRHA 55 55 -
ANPPNAELTSVANTPQSYWL O53607 111 111 -
NAAAEPGGDRIADEPTGVWL O86730 183 183 -
DHRAPLIAERIGSQPQAVWF GUN1_STRSQ 97 97 -
TGAMATAAAAVAKVPSFMWL GUX2_TRIRE 141 141 -
DSRAARIQSSVANNLAARWF Q50901 225 225 -
DPALAAKMRTVAGQPTAVWM GUXA_CELFI 74 74 -
KAAAEPGGSAVANESTAVWL Q60029 197 197 -
DFRAAVIREKIASQPQARWY Q53488 58 58 -

Motif 2 width=14
Element Seqn Id St Int Rpt
KIPILVVYNAPGRD GUN2_THEFU 97 23 -
KTVVFVLYMIPTRD P78721 193 18 -
QLVQIVVYDLPDRD GUX3_AGABI 156 20 -
KIPIMVVYAMPNRD GUNA_MICBI 100 23 -
KTVVFVLYMIPTRD P78720 194 18 -
KTVVFIMYMIPTRD Q12646 169 18 -
KTPMLVVYAIPGRD GUNA_CELFI 234 24 -
QLVQIVIYDLPDRD Q02321 178 26 -
YAGQFVVYDLPNRD GUNB_FUSOX 177 25 -
KLPILVAYNIYNRD GUN1_STRHA 97 22 -
AMPVLTLYGIPHRD O53607 155 24 -
MVVQLVIYNLPGRD O86730 233 30 -
QLPVVVPYMIPFRD GUN1_STRSQ 141 24 -
YAGQFVVYDLPDRD GUX2_TRIRE 186 25 -
KLPVLVAYNIPGRD Q50901 267 22 -
LVFNLVIYDLPGRD GUXA_CELFI 126 32 -
LTIQVVIYNLPGRD Q60029 251 34 -
QIPVLSVYEITNRD Q53488 101 23 -

Motif 3 width=16
Element Seqn Id St Int Rpt
YRSWIDEFAAGLKNRP GUN2_THEFU 125 14 -
YQGYVNSIYNTINQYP P78721 222 15 -
YKNYVDQIAAQIKQFP GUX3_AGABI 189 19 -
YRAWIDEIAAGLRNRP GUNA_MICBI 128 14 -
YKGYINNIYNTSNQYK P78720 223 15 -
YKGYVDNIARTIRSYP Q12646 198 15 -
YARWVDTVAQGIKGNP GUNA_CELFI 261 13 -
YENYIDQIVAQIQQFP Q02321 211 19 -
YKAYIAKIKGILQNYS GUNB_FUSOX 210 19 -
YADWIARFAGGIAARP GUN1_STRHA 126 15 -
YRGWIDAVASGLGSSP O53607 183 14 -
KTEYIDPIAEILSDSK O86730 265 18 -
YAEWSGLFAAGLGSEP GUN1_STRSQ 169 14 -
YKNYIDTIRQIVVEYS GUX2_TRIRE 219 19 -
YRTWISSFAAAIGNRP Q50901 295 14 -
KSEYIDPIADLLDNPE GUXA_CELFI 160 20 -
YVNGVGYALRKLGEIP Q60029 339 74 -
YQTWVSNFARGLGNQT Q53488 129 14 -

Motif 4 width=14
Element Seqn Id St Int Rpt
QARIYFDAGHSAWH GUN2_THEFU 181 40 -
NVRVYLDAAHGGWL P78721 284 46 -
GVTMYIDAGHAGWL GUX3_AGABI 251 46 -
QAKVYFDAGHDAWV GUNA_MICBI 185 41 -
HVKVYLDAAHGAWL P78720 285 46 -
NVSVYLDAAHGAWL Q12646 260 46 -
GARVYIDAGHAKWL GUNA_CELFI 312 35 -
GVYMYMDAGHAGWL Q02321 273 46 -
NVSMYLDAGHGGWL GUNB_FUSOX 272 46 -
NTWVYMDAGNPRWA GUN1_STRHA 182 40 -
AAAVYVDAGHSRWL O53607 238 39 -
NVYNYVDAGHHGWL O86730 337 56 -
EARVYYDVGHSAWH GUN1_STRSQ 225 40 -
NVAMYLDAGHAGWL GUX2_TRIRE 281 46 -
NTWAYLDAGNALWI Q50901 352 41 -
NVYNYIDIGHSGWL GUXA_CELFI 225 49 -
NVYNYIDAAHHGWI Q60029 355 0 -
NAKVYLDGGHSTWN Q53488 185 40 -

Motif 5 width=11
Element Seqn Id St Int Rpt
AHGIATNTSNY GUN2_THEFU 212 17 -
IRGISTNVSNY P78721 320 22 -
LRGIATNVANF GUX3_AGABI 290 25 -
ADGIALNVSNY GUNA_MICBI 216 17 -
LRGISTNVSNY P78720 321 22 -
IRGLSTNISNY Q12646 296 22 -
AVGFALNTSNY GUNA_CELFI 342 16 -
IKGLATNVANY Q02321 312 25 -
VRGLVTNVSNY GUNB_FUSOX 311 25 -
AHGFSLNVSNY GUN1_STRHA 212 16 -
ARGFSLNVSNF O53607 268 16 -
VHGFIVNTANY O86730 377 26 -
GAGIATNISNY GUN1_STRSQ 256 17 -
LRGLATNVANY GUX2_TRIRE 320 25 -
VRGFALNVSNF Q50901 382 16 -
IDGFVSDVANT GUXA_CELFI 265 26 -
VHGFISNTANY Q60029 395 26 -
ADGFFTNVSNF Q53488 215 16 -

Motif 6 width=14
Element Seqn Id St Int Rpt
LRAVIDTSRNGNGP GUN2_THEFU 244 21 -
MKFIVDTSRNGRNP P78721 355 24 -
AHFIVDQGRSGVQN GUX3_AGABI 338 37 -
LRAVIDTSRNGNGP GUNA_MICBI 248 21 -
LKFIVDTSRNGANV P78720 356 24 -
MHFIVDTGRNGVTI Q12646 331 24 -
KKFVIDTSRNGNGS GUNA_CELFI 372 19 -
ATFIVDQGRSGVQN Q02321 360 37 -
VKFIVDQGRSGKQP GUNB_FUSOX 360 38 -
KPFVVDTSRNGNGS GUN1_STRHA 247 24 -
SHYVIDTSRNGAGP O53607 298 19 -
LGMLIDTSRNGWGG O86730 440 52 -
LGAVVDTSRNGNGP GUN1_STRSQ 287 20 -
AFFITDQGRSGKQP GUX2_TRIRE 369 38 -
KPFVVDTSRNGNGS Q50901 417 24 -
IGMLVDTSRNGWGG GUXA_CELFI 329 53 -
IGMLIDTSRNGWGG Q60029 458 52 -
KRQVIDTSRNGGAA Q53488 250 24 -

Motif 7 width=21
Element Seqn Id St Int Rpt
GNEWCDPSGRAIGTPSTTNTG GUN2_THEFU 259 1 -
SATWCNLKGAGLGARPQANPD P78721 370 1 -
WGDWCNVKGAGFGQRPTTNTG GUX3_AGABI 356 4 -
GSEWCDPPGRATGTWSTTDTG GUNA_MICBI 263 1 -
SGTWCNFKGAGLGQRPKGNPN P78720 376 6 -
SGTWCNLVGTGLGERPRGNPN Q12646 346 1 -
NGEWCNPRGRALGERPVAVND GUNA_CELFI 386 0 -
WGDWCNIKGAGFGTRPTTNTG Q02321 378 4 -
QGDWCNAKGTGFGLRPSTNTG GUNB_FUSOX 379 5 -
NGEWCNPSGRRIGTPTRTGGG GUN1_STRHA 261 0 -
PLNWCNPSGRALGAPPTTATA O53607 316 4 -
LGNWCNQSGAGLGERPQASPA O86730 481 27 -
GSEWCDPPGRLVGNNPTVNPG GUN1_STRSQ 302 1 -
WGDWCNVIGTGFGIRPSANTG GUX2_TRIRE 388 5 -
NGEWCNPAGRKIGTTNQVGVG Q50901 431 0 -
RGAWCNPLGAGIGRFPEATPS GUXA_CELFI 370 27 -
PGNWCNQAGAGLGERPTVNPA Q60029 499 27 -
DWCADDNTDRRIGQYPTTNTG Q53488 265 1 -

Motif 8 width=15
Element Seqn Id St Int Rpt
IDAFLWIKLPGEADG GUN2_THEFU 283 3 -
LDAYVWIKTPGESDS P78721 396 5 -
IDAIVWVKPGGECDG GUX3_AGABI 380 3 -
IDAFLWIKPPGEADG GUNA_MICBI 287 3 -
LDAYMWIKTPGEADG P78720 403 6 -
LDAYMWLKTPGESDG Q12646 372 5 -
LDALLWVKLPGESDG GUNA_CELFI 410 3 -
IDSIVWVKPGGECDG Q02321 402 3 -
ADAFVWVKPGGESDG GUNB_FUSOX 403 3 -
AEMLLWIKTPGESDG GUN1_STRHA 282 0 -
ADAYLWIKRPGESDG O53607 340 3 -
IDAYVWMKPPGESDG O86730 504 2 -
VDAFLWIKLPGELDG GUN1_STRSQ 326 3 -
LDSFVWVKPGGECDG GUX2_TRIRE 412 3 -
AEMTVWIKVPGDSDG Q50901 453 1 -
LDAFVWIKPPGESDG GUXA_CELFI 397 6 -
VDAYVWVKPPGESDG Q60029 522 2 -
IDAYLWVKPPGEADG Q53488 289 3 -