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PR00706

Identifier
PYROGLUPTASE  [View Relations]  [View Alignment]  
Accession
PR00706; PR00140
No. of Motifs
5
Creation Date
01-JUN-1997  (UPDATE 07-JUN-1999)
Title
Pyroglutamyl peptidase I (C15) family signature
Database References

INTERPRO; IPR000816
Literature References
1. RAWLINGS, N.D. AND BARRETT, A.J.
Families of cysteine peptidases.
METHODS ENZYMOL. 244 461-486 (1994).
 
2. AWADE, A.C., CLEUZIAT, P., GONZALES, T. AND ROBERT-BAUDOUY, J.
Pyrrolidone carboxyl peptidase (Pcp): an enzyme that removes
pyroglutamic acid (pGlu) from pGlu-peptides and pGlu-proteins.
PROTEINS 20 34-51 (1994).
 
3. AWADE, A., CLEUZIAT, P., GONZALES, T. AND ROBERT-BAUDOUY, J.
Characterization of the pcp gene encoding the pyrrolidone carboxyl peptidase
of Bacillus subtilis.
FEBS LETT. 305 67-73 (1992).
 
4. GONZALES, T. AND ROBERT-BAUDOUY, J.
Characterization of the pcp gene of Pseudomonas fluorescens and of its 
product, pyrrolidone carboxyl peptidase (PCP).
J.BACTERIOL. 176 2569-2576 (1994).

Documentation
Cysteine protease activity is dependent on an active dyad of cysteine and
histidine, the order and spacing of these residues varying in the 20 or so
known families. Families C1, C2 and C10 are loosely termed papain-like, and 
nearly half of all cysteine proteases are found exclusively in viruses [1].
 
Pyrrolidone carboxyl peptidase (Pcp or PYRase) is an exopeptidase that
hydrolytically removes the pGlu from pGlu-peptides or pGlu-proteins [2,3].
PYRase has been found in a variety of bacterial, plant and animal tissues.
At least two different classes have been characterised, the first
containing bacterial and animal type I PYRases, and the second containing
animal type II and serum PYRases. Type I and bacterial PYRases are soluble
enzymes, while type II PYRases are membrane-bound. The primary application
of PYRase has been its utilisation for protein or peptide sequencing, and
bacterial diagnosis [3]. The conserved residues Cys-144 and His-168 have 
been identified by inhibition and mutagenesis studies [2,4].
 
PYROGLUPTASE is a 5-element fingerprint that provides a signature for the
pyroglutamyl peptidase I (C15) protease family. The fingerprint was derived
from an initial alignment of 4 sequences: the motifs were drawn from 
conserved regions spanning the N-terminal two-thirds of the alignment -
motif 4 contains the active Cys residue and motif 5 contains the active His.
Two iterations on OWL29.3 were required to reach convergence, at which point
a true set comprising 5 sequences was identified.
 
An update on SPTR37_9f identified a true set of 9 sequences, and 1
partial match.
Summary Information
   9 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
599999
400000
300000
210010
12345
True Positives
O58321        O73944        O87765        PCP_BACAM     
PCP_BACSU PCP_PSEFL PCP_STRPY PCP_THELI
Q53596
True Positive Partials
Codes involving 2 elements
O07930
Sequence Titles
O58321      PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - PYROCOCCUS HORIKOSHII. 
O73944 PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - PYROCOCCUS FURIOSUS.
O87765 PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - LACTOCOCCUS LACTIS (SUBSP. CREMORIS) (STREPTOCOCCUS CREMORIS).
PCP_BACAM PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - BACILLUS AMYLOLIQUEFACIENS.
PCP_BACSU PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - BACILLUS SUBTILIS.
PCP_PSEFL PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - PSEUDOMONAS FLUORESCENS.
PCP_STRPY PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - STREPTOCOCCUS PYOGENES.
PCP_THELI PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) - THERMOCOCCUS LITORALIS.
Q53596 PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - STAPHYLOCOCCUS AUREUS.

O07930 PYRROLIDONE-CARBOXYLATE PEPTIDASE (EC 3.4.19.3) (5-OXOPROLYL- PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) - MYCOBACTERIUM TUBERCULOSIS, AND MYCOBACTERIUM BOVIS.
Scan History
OWL29_3    2  100  NSINGLE    
SPTR37_9f 2 10 NSINGLE
Initial Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
VLLTGFDPFGGETVNPSWEAVKRL PCP_BACAM 5 5 -
VLITGFDPFDKETVNPSWEAAKRL PCP_BACSU 5 5 -
ILVTGFDPFGGEAINPALEAIKKL PCP_STRPY 3 3 -
VLLTGFEPFDQDPVNPSWEAVRQL PCP_PSEFL 4 4 -

Motif 2 width=13
Element Seqn Id St Int Rpt
PDIIICVGQAGGR PCP_BACAM 63 34 -
PDIVICVGQAGGR PCP_BACSU 63 34 -
PDAVLCIGQAGGR PCP_STRPY 60 33 -
PAMVIATGLGPGR PCP_PSEFL 63 35 -

Motif 3 width=21
Element Seqn Id St Int Rpt
ERVAINLNEARIPDNEGNQPV PCP_BACAM 81 5 -
ERVAINLADARIPDNEGHQPI PCP_BACSU 81 5 -
ERVAINQDDARIPDNEGNQPI PCP_STRPY 78 5 -
ERVAININDARIPDNLGEQPI PCP_PSEFL 81 5 -

Motif 4 width=17
Element Seqn Id St Int Rpt
AAVSYTAGTFVCNHLFY PCP_BACAM 133 31 -
AAVSYTAGTFVCNYLFY PCP_BACSU 133 31 -
ASVSNTAGTFVCNHLMY PCP_STRPY 130 31 -
ASVSQTAGTFVCNQVFY PCP_PSEFL 133 31 -

Motif 5 width=13
Element Seqn Id St Int Rpt
RGGFIHIPYIPEQ PCP_BACAM 163 13 -
RGGFIHIPYIPQQ PCP_BACSU 163 13 -
KAGFMHIPFMMEQ PCP_STRPY 160 13 -
RSGFIHVPFLPEQ PCP_PSEFL 161 11 -
Final Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
VLLTGFDPFGGETVNPSWEAVKRL PCP_BACAM 5 5 -
VLITGFDPFDKETVNPSWEAAKRL PCP_BACSU 5 5 -
ILVTGFDPFGGEAINPALEAIKKL PCP_STRPY 3 3 -
ILVTGFDPFGDDKINPAIEAVKRL O87765 3 3 -
ILVTGFAPFDNQDINPSWEAVTQL Q53596 3 3 -
VLLTGFEPFDQDPVNPSWEAVRQL PCP_PSEFL 4 4 -
ILLTGFEPFGGDDKNPTMDIVEAL O58321 3 3 -
VLVTGFEPFGGEKINPTERIAKDL O73944 3 3 -
VLITGFEPFGGDSKNPTEQIAKYF PCP_THELI 4 4 -

Motif 2 width=13
Element Seqn Id St Int Rpt
PDIIICVGQAGGR PCP_BACAM 63 34 -
PDIVICVGQAGGR PCP_BACSU 63 34 -
PDAVLCIGQAGGR PCP_STRPY 60 33 -
PDYVLSIGQAGGR O87765 60 33 -
YDVVLAIGQAGGR Q53596 60 33 -
PAMVIATGLGPGR PCP_PSEFL 63 35 -
PDITINLGLAPGR O58321 58 31 -
PDIAIHVGLAPGR O73944 61 34 -
PEIVINLGLAPTY PCP_THELI 62 34 -

Motif 3 width=21
Element Seqn Id St Int Rpt
ERVAINLNEARIPDNEGNQPV PCP_BACAM 81 5 -
ERVAINLADARIPDNEGHQPI PCP_BACSU 81 5 -
ERVAINQDDARIPDNEGNQPI PCP_STRPY 78 5 -
ERVAINLDDGRIQDNAGYQPL O87765 78 5 -
ERVAINIDDARIPDNDDFQPI Q53596 78 5 -
ERVAININDARIPDNLGEQPI PCP_PSEFL 81 5 -
ERVAVNMIDARIPDNDGEQPK O58321 76 5 -
ERIAVNAIDARIPDNEGKKIE O73944 79 5 -
ERIAVNIIDARIPDNDGYQPI PCP_THELI 80 5 -

Motif 4 width=17
Element Seqn Id St Int Rpt
AAVSYTAGTFVCNHLFY PCP_BACAM 133 31 -
AAVSYTAGTFVCNYLFY PCP_BACSU 133 31 -
ASVSNTAGTFVCNHLMY PCP_STRPY 130 31 -
SAVSNTAGTYVCNHIFY O87765 130 31 -
GALSNSAGTFVCNHVLY Q53596 130 31 -
ASVSQTAGTFVCNQVFY PCP_PSEFL 133 31 -
AVLSYTAGTYLCNFAMY O58321 128 31 -
AYISNSAGLYLCNYVMY O73944 131 31 -
ATISYSAGTYLCNYVMF PCP_THELI 132 31 -

Motif 5 width=13
Element Seqn Id St Int Rpt
RGGFIHIPYIPEQ PCP_BACAM 163 13 -
RGGFIHIPYIPQQ PCP_BACSU 163 13 -
KAGFMHIPFMMEQ PCP_STRPY 160 13 -
KAGFMHIPFLPEQ O87765 160 13 -
RFGFIHVPYIPEQ Q53596 160 13 -
RSGFIHVPFLPEQ PCP_PSEFL 161 11 -
IAGFIHVPYTPDQ O58321 158 13 -
MSGFIHVPYIPEQ O73944 161 13 -
KAGFIHVPYTPDQ PCP_THELI 162 13 -