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PR00690

Identifier
ADHESNFAMILY  [View Relations]  [View Alignment]  
Accession
PR00690
No. of Motifs
6
Creation Date
10-APR-1997  (UPDATE 28-JUL-1999)
Title
Adhesin family signature
Database References
PRINTS; PR00691 ADHESINB
PROSITE; PS00013 PROKAR_LIPOPROTEIN
PFAM; PF01297 Lipoprotein_4
INTERPRO; IPR001987
Literature References
1. SAMPSON, J.S., O'CONNOR, S.P., STINSON, A.R., THARPE, J.A. AND RUSSELL, H.
Cloning and nucleotide sequence analysis of psaA, the Streptococcus
pneumoniae gene encoding a 37-kilodalton protein homologous to previously
reported Streptococcus sp. adhesins.
INFECT.IMMUN. 62(1) 319-324 (1994).
 
2. GANESHKUMAR, N., HANNAM, P.M., KOLENBRANDER, P.E. AND MCBRIDE, B.C.
Nucleotide sequence of a gene coding for a saliva-binding protein (ssaB)
from Streptococcus sanguis 12 and possible role of the protein in
coaggregation with Actinomyces. 
INFECT.IMMUN. 59(3) 1093-1099 (1991).

Documentation
The Streptococcus pneumoniae psaA gene encodes a protein with significant
similarity to previously-reported Streptococcal proteins, SsaB (80%
similarity) and FimA (92.3% similarity), from S.sanguis and S.parasanguis
[1]. These homologues are associated with bacterial adhesion, and PsaA 
may play a similar role [1].
 
The SsaB protein has a putative hydrophobic 19-amino-acid signal sequence
yielding a 32,620-Mr secreted protein [2]. SsaB is hydrophilic and appears
not to have a hydrophobic membrane anchor in its C-terminal region. A high
degree of similarity exists between S.sanguis ssaB and type 1 fimbrial
genes [2]. Comparison of the gene products reveals close similarity of the
two proteins. It is thought that ssaB adhesion may play a role in oral
colonisation by binding either to a receptor on saliva or to a receptor
on Actinomyces. 
 
ADHESNFAMILY is a 6-element fingerprint that provides a signature for
the adhesins and related periplasmic binding proteins. The fingerprint was
derived from an initial alignment of 15 sequences: the motifs were drawn
from short conserved regions spanning the full alignment length. A single
iteration on OWL29.2 was required to reach convergence, no further sequences
being identified beyond the starting set. Two partial matches were found,
YEBL_ECOLI and D908289, both of which match motifs 2 and 3.
 
An update on SPTR37_9f identified a true set of 15 sequences, and 6
partial matches.
Summary Information
  15 codes involving  6 elements
0 codes involving 5 elements
2 codes involving 4 elements
0 codes involving 3 elements
4 codes involving 2 elements
Composite Feature Index
6151515151515
5000000
4222200
3000000
2133100
123456
True Positives
ADHS_STRGC    ADHS_STRPA    ADHS_STRPN    ADHS_STRSA    
O34385 P72415 P72538 P96116
Q47723 Q47869 Q53891 Q55280
Q56329 Q56952 Q57449
True Positive Partials
Codes involving 4 elements
O34966 O83077
Codes involving 2 elements
O67917 O84420 Q54914 ZNUA_ECOLI
Sequence Titles
ADHS_STRGC  COAGGREGATION-MEDIATING ADHESIN PRECURSOR - STREPTOCOCCUS GORDONII CHALLIS. 
ADHS_STRPA ADHESIN B PRECURSOR (SALIVA-BINDING PROTEIN) - STREPTOCOCCUS PARASANGUIS.
ADHS_STRPN ADHESION PROTEIN PRECURSOR - STREPTOCOCCUS PNEUMONIAE.
ADHS_STRSA ADHESIN B PRECURSOR (SALIVA-BINDING PROTEIN) - STREPTOCOCCUS SANGUIS.
O34385 YTGA - BACILLUS SUBTILIS.
P72415 LIPOPROTEIN - STAPHYLOCOCCUS EPIDERMIDIS.
P72538 SURFACE ADHESIN A PRECURSOR - STREPTOCOCCUS PNEUMONIAE.
P96116 TROA PRECURSOR - TREPONEMA PALLIDUM.
Q47723 ENDOCARDITIS SPECIFIC ANTIGEN - ENTEROCOCCUS FAECALIS (STREPTOCOCCUS FAECALIS).
Q47869 EWLA - ERYSIPELOTHRIX RHUSIOPATHIAE.
Q53891 SCBA - STREPTOCOCCUS CRISTATUS.
Q55280 PERIPLASMIC-BINDING PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
Q56329 TROMP1 - TREPONEMA PALLIDUM.
Q56952 YFEA - YERSINIA PESTIS.
Q57449 HYPOTHETICAL PROTEIN HI0362 PRECURSOR - HAEMOPHILUS INFLUENZAE.

O34966 YCDH - BACILLUS SUBTILIS.
O83077 ABC TRANSPORTER, PERIPLASMIC BINDING PROTEIN - TREPONEMA PALLIDUM.

O67917 ADHESION PROTEIN - AQUIFEX AEOLICUS.
O84420 SOLUTE-BINDING PROTEIN - CHLAMYDIA TRACHOMATIS.
Q54914 ORF 1 AND ORF2 5' REGION - STREPTOCOCCUS PYOGENES.
ZNUA_ECOLI HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA PRECURSOR - ESCHERICHIA COLI.
Scan History
OWL29_2    1  100  NSINGLE    
SPTR37_9f 2 200 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
KKKVLTTFTVLADMVQNVA S55045 54 54 -
KLNVVATNSIIADITKNIA ADHS_STRGC 33 33 -
KLKVVTTNSILADITKNIA ADHS_STRPA 32 32 -
KLKVVATNSIIADITKNIA ADHS_STRSA 32 32 -
KLKVVTTNSILADITKNIA ADHS_STRPN 33 33 -
KLKVVATNSIIADITKNIA SPU407861 32 32 -
KLAIVTTNSILSDLVKNVG EFU03756 30 30 -
KFKVVTTFTVIQDIAQNVA G64063 23 23 -
KPLVVTTIGMIADAVKNIA TPU552141 33 33 -
KPLVVTTIGMIADAVKNIA TPU16363 43 43 -
KLKVVTTNSILYDMVKRVG SEABCTS2 32 32 -
KFKVVTTFTIIQDIAQNIA YPU50597 52 52 -
KLNVVATNSIIADITKNIA SCU465421 33 33 -
KLKVVATNSIIADITKNIA SPU53509 32 32 -
KINVVATTTMIKDLVEIIG ERU52850 32 32 -

Motif 2 width=14
Element Seqn Id St Int Rpt
GKDPHEYEPLPEDV ADHS_STRPA 63 12 -
GQDPHKYEPLPEDV ADHS_STRGC 64 12 -
GKDPHEYEPLPEDV ADHS_STRSA 63 12 -
GQDPHEYEPLPEDV ADHS_STRPN 64 12 -
GQDPHEYEPLPEDV SPU53509 63 12 -
GQDPHEYEPLPEDV SCU465421 64 12 -
GAEIHDYQPTPRDI YPU50597 83 12 -
GQDPHEYEVKPKDI SEABCTS2 63 12 -
GVDPHLYTATAGDV TPU16363 74 12 -
GVDPHLYTATAGDV TPU552141 64 12 -
GAEIHEYEPTPKDI G64063 54 12 -
GTDPHEYEPLPEDI EFU03756 61 12 -
GAEIHGYEPTPSDI S55045 85 12 -
GVDPHLYKAKPSDV ERU52850 63 12 -
GQDPHDYEPLAEDV SPU407861 63 12 -

Motif 3 width=18
Element Seqn Id St Int Rpt
IAKASEADILFFNGLNLE EFU03756 74 -1 -
IVKAQDADLILYNGMNLE S55045 98 -1 -
VKAIQEADVVAFNGVHLE ERU52850 76 -1 -
VEWLGNADLILYNGLHLE TPU552141 77 -1 -
VEWLGNADLILYNGLHLE TPU16363 87 -1 -
IKALTDADVVFYNGLNLE SEABCTS2 76 -1 -
IVKAQSADLILWNGMNLE YPU50597 96 -1 -
VKKTSQADLIFYNGINLE SCU465421 77 -1 -
VKKTSEADLIFYNGINLE SPU53509 76 -1 -
VKKTSEADLIFYNGINLE SPU407861 76 -1 -
VKKTSKADLIFYNGINLE ADHS_STRGC 77 -1 -
VKKTSQADLIFYNGINLE ADHS_STRPN 77 -1 -
VKKTSQADLIFYNGINLE ADHS_STRSA 76 -1 -
VKKTSQADLIFYNGINLE ADHS_STRPA 76 -1 -
IVKAQSADLILWNGLNLE G64063 67 -1 -

Motif 4 width=22
Element Seqn Id St Int Rpt
IPAEKKMIVTSEGCFKYFSKAY ADHS_STRPN 195 100 -
IPAEKKLIVTSEGAFKYFSKAY SPU407861 194 100 -
IPAEKKLIVTSEGAFKYFSKAY SPU53509 194 100 -
IPGEKKMIVTSEGCFKYFSKAY SCU465421 195 100 -
IPAEQRWLVTSEGAFSYLAKDY YPU50597 207 93 -
IPKNQRAMMTSEGAFKYFAQQF SEABCTS2 195 101 -
LPAERRVLVTAHDAFGYFSRAY TPU16363 198 93 -
LPAERRVLVTAHDAFGYFSRAY TPU552141 188 93 -
IPEAQRWLVTSEGAFSYLAKDY G64063 178 93 -
IPDDKKLLVTSEGAFKYFSKAY EFU03756 192 100 -
VPANQRFLVSCEGAFSYLARDY S55045 209 93 -
IPEQQRVLVTAHDAFAYFGRYF ERU52850 189 95 -
IPEEKKMIVTSEGCFKYFSKAY ADHS_STRSA 194 100 -
IPEDKKMIVTSEGCFKYFSKAY ADHS_STRPA 194 100 -
IPEEKKMIVTSEGCPKYFSKAY ADHS_STRGC 195 100 -

Motif 5 width=19
Element Seqn Id St Int Rpt
KIPVVFSESTISDKPAKQV YPU50597 260 31 -
KVPSLFVESSVDDRPMKTV SCU465421 248 31 -
KVPSLFVESSVDDRPMKTV SPU53509 247 31 -
KVPSLFVDSSVDDRPMKTV SPU407861 247 31 -
KIKAIYTESSVPKKTIESL ERU52850 242 31 -
NVPTIFCESTVSDKGQKQV S55045 262 31 -
KAPVLFVETSVDKRSMERV EFU03756 245 31 -
KVPSLFVESSVDERPMKTV ADHS_STRPN 248 31 -
KVPSLFVESSVDDRPMKTV ADHS_STRSA 247 31 -
KVPALFVESSVDERPMKTV ADHS_STRPA 247 31 -
KVPSLFVESSVDDRPMKTV ADHS_STRGC 248 31 -
NIPVVFSESTISAKPAQQV G64063 231 31 -
KLPAIFIESSIPHKNVEAL TPU552141 241 31 -
KLPAIFIESSIPHKNVEAL TPU16363 251 31 -
HLKHLLVETSVDKKAMQSL SEABCTS2 248 31 -

Motif 6 width=20
Element Seqn Id St Int Rpt
IYGEVFTDSIGKEGTKGDSY SEABCTS2 274 7 -
IGGELFSDAMGDAGTSEGTY TPU552141 272 12 -
YGGVLYVDSLSAKNGPVPTY G64063 257 7 -
IYDTLFTDSLAKEGTEGDTY EFU03756 271 7 -
FGGNLYVDSLSTEEGPVPTF S55045 288 7 -
IGGEIYSDSLKEDASYIETY ERU52850 273 12 -
IYAQIFTDSIAEQGKEGDSY SPU53509 273 7 -
IYAQIFTDSIAEQGKEGDRY SPU407861 273 7 -
IHAKIFTDSIADQGEEGDTY ADHS_STRSA 273 7 -
IYAKIFTDSIAKEGEKGDSY ADHS_STRPA 273 7 -
IYAKIFTDSIAEKGEDGDSY ADHS_STRGC 274 7 -
IFAKIFTDSIAKEGEEGDSY ADHS_STRPN 274 7 -
IYAKIFTDSVAEKGEEGDSY SCU465421 274 7 -
YGGVLYVDSLSGEKGPVPTY YPU50597 286 7 -
IGGELFSDAMGDAGTSEGTY TPU16363 282 12 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
KLKVVATNSIIADITKNIA P72538 32 32 -
KLKVVTTNSILADITKNIA ADHS_STRPN 33 33 -
KLKVVATNSIIADITKNIA ADHS_STRSA 32 32 -
KLNVVATNSIIADITKNIA Q53891 33 33 -
KLKVVTTNSILADITKNIA ADHS_STRPA 32 32 -
KLNVVATNSIIADITKNIA ADHS_STRGC 33 33 -
KLAIVTTNSILSDLVKNVG Q47723 30 30 -
KPLVVTTIGMIADAVKNIA P96116 33 33 -
KPLVVTTIGMIADAVKNIA Q56329 43 43 -
KFKVVTTFTIIQDIAQNIA Q56952 52 52 -
KLKVVTTNSILYDMVKRVG P72415 32 32 -
KFKVVTTFTVIQDIAQNVA Q57449 23 23 -
KKKVLTTFTVLADMVQNVA Q55280 54 54 -
QLQVTATTSQIADAAENIG O34385 31 31 -
KINVVATTTMIKDLVEIIG Q47869 32 32 -

Motif 2 width=14
Element Seqn Id St Int Rpt
GQDPHEYEPLPEDV P72538 63 12 -
GQDPHEYEPLPEDV ADHS_STRPN 64 12 -
GKDPHEYEPLPEDV ADHS_STRSA 63 12 -
GQDPHEYEPLPEDV Q53891 64 12 -
GKDPHEYEPLPEDV ADHS_STRPA 63 12 -
GQDPHKYEPLPEDV ADHS_STRGC 64 12 -
GTDPHEYEPLPEDI Q47723 61 12 -
GVDPHLYTATAGDV P96116 64 12 -
GVDPHLYTATAGDV Q56329 74 12 -
GAEIHDYQPTPRDI Q56952 83 12 -
GQDPHEYEVKPKDI P72415 63 12 -
GAEIHEYEPTPKDI Q57449 54 12 -
GAEIHGYEPTPSDI Q55280 85 12 -
GVDPHLYKASQGDT O34385 62 12 -
GVDPHLYKAKPSDV Q47869 63 12 -

Motif 3 width=18
Element Seqn Id St Int Rpt
VKKTSEADLIFYNGINLE P72538 76 -1 -
VKKTSQADLIFYNGINLE ADHS_STRPN 77 -1 -
VKKTSQADLIFYNGINLE ADHS_STRSA 76 -1 -
VKKTSQADLIFYNGINLE Q53891 77 -1 -
VKKTSQADLIFYNGINLE ADHS_STRPA 76 -1 -
VKKTSKADLIFYNGINLE ADHS_STRGC 77 -1 -
IAKASEADILFFNGLNLE Q47723 74 -1 -
VEWLGNADLILYNGLHLE P96116 77 -1 -
VEWLGNADLILYNGLHLE Q56329 87 -1 -
IVKAQSADLILWNGMNLE Q56952 96 -1 -
IKALTDADVVFYNGLNLE P72415 76 -1 -
IVKAQSADLILWNGLNLE Q57449 67 -1 -
IVKAQDADLILYNGMNLE Q55280 98 -1 -
TKKLMSADVVLYSGLHLE O34385 75 -1 -
VKAIQEADVVAFNGVHLE Q47869 76 -1 -

Motif 4 width=22
Element Seqn Id St Int Rpt
IPAEKKLIVTSEGAFKYFSKAY P72538 194 100 -
IPAEKKMIVTSEGCFKYFSKAY ADHS_STRPN 195 100 -
IPEEKKMIVTSEGCFKYFSKAY ADHS_STRSA 194 100 -
IPGEKKMIVTSEGCFKYFSKAY Q53891 195 100 -
IPEDKKMIVTSEGCFKYFSKAY ADHS_STRPA 194 100 -
IPEEKKMIVTSEGCPKYFSKAY ADHS_STRGC 195 100 -
IPDDKKLLVTSEGAFKYFSKAY Q47723 192 100 -
LPAERRVLVTAHDAFGYFSRAY P96116 188 93 -
LPAERRVLVTAHDAFGYFSRAY Q56329 198 93 -
IPAEQRWLVTSEGAFSYLAKDY Q56952 207 93 -
IPKNQRAMMTSEGAFKYFAQQF P72415 195 101 -
IPEAQRWLVTSEGAFSYLAKDY Q57449 178 93 -
VPANQRFLVSCEGAFSYLARDY Q55280 209 93 -
IPEKSRVLVTAHDAFAYFGNEY O34385 187 94 -
IPEQQRVLVTAHDAFAYFGRYF Q47869 189 95 -

Motif 5 width=19
Element Seqn Id St Int Rpt
KVPSLFVESSVDDRPMKTV P72538 247 31 -
KVPSLFVESSVDERPMKTV ADHS_STRPN 248 31 -
KVPSLFVESSVDDRPMKTV ADHS_STRSA 247 31 -
KVPSLFVESSVDDRPMKTV Q53891 248 31 -
KVPALFVESSVDERPMKTV ADHS_STRPA 247 31 -
KVPSLFVESSVDDRPMKTV ADHS_STRGC 248 31 -
KAPVLFVETSVDKRSMERV Q47723 245 31 -
KLPAIFIESSIPHKNVEAL P96116 241 31 -
KLPAIFIESSIPHKNVEAL Q56329 251 31 -
KIPVVFSESTISDKPAKQV Q56952 260 31 -
HLKHLLVETSVDKKAMQSL P72415 248 31 -
NIPVVFSESTISAKPAQQV Q57449 231 31 -
NVPTIFCESTVSDKGQKQV Q55280 262 31 -
QIKAVFVESSVSEKSINAV O34385 240 31 -
KIKAIYTESSVPKKTIESL Q47869 242 31 -

Motif 6 width=20
Element Seqn Id St Int Rpt
IYAQIFTDSIAEQGKEGDSY P72538 273 7 -
IFAKIFTDSIAKEGEEGDSY ADHS_STRPN 274 7 -
IHAKIFTDSIADQGEEGDTY ADHS_STRSA 273 7 -
IYAKIFTDSVAEKGEEGDSY Q53891 274 7 -
IYAKIFTDSIAKEGEKGDSY ADHS_STRPA 273 7 -
IYAKIFTDSIAEKGEDGDSY ADHS_STRGC 274 7 -
IYDTLFTDSLAKEGTEGDTY Q47723 271 7 -
IGGELFSDAMGDAGTSEGTY P96116 272 12 -
IGGELFSDAMGDAGTSEGTY Q56329 282 12 -
YGGVLYVDSLSGEKGPVPTY Q56952 286 7 -
IYGEVFTDSIGKEGTKGDSY P72415 274 7 -
YGGVLYVDSLSAKNGPVPTY Q57449 257 7 -
FGGNLYVDSLSTEEGPVPTF Q55280 288 7 -
IGGQLYSDAMGEKGTKEGTY O34385 271 12 -
IGGEIYSDSLKEDASYIETY Q47869 273 12 -