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PR00665

Identifier
OXYTOCINR  [View Relations]  [View Alignment]  
Accession
PR00665
No. of Motifs
7
Creation Date
21-FEB-1997  (UPDATE 07-JUN-1999)
Title
Oxytocin receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00896 VASOPRESSINR
INTERPRO; IPR002062
GCRDB; GCR_0026; GCR_0033
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Vasopressin and oxytocin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.284-291.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Vasopressin and oxytocin are members of the neurohypophyseal hormone family
found in all mammalian species [6]. They are present in high levels in the
posterior pituitary. Oxytocin stimulates contraction of uterine smooth 
muscle, and stimulates milk secretion in response to suckling by inducing
contraction of myoepithelial cells in the mammary gland [6]. Clinically, it
is used to induce labour and promote lactation.
 
Oxytocin receptors are found in uterine smooth muscle, myoepithelial
cells in the mammary gland, and in the pituitary [6]. Activation of
phosphoinositide metabolism is effected via a pertussis-toxin-insensitive
G protein, probably of the Gq/G11 class [6].
 
OXYTOCINR is a 7-element fingerprint that provides a signature for the
oxytocin receptors. The fingerprint was derived from an initial alignment
of 4 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment 
that characterise the oxytocin receptors but distinguish them from the
rest of the rhodopsin-like superfamily - motifs 1 and 2 lie at the
N-terminus; motif 3 spans the first cytoplasmic loop; motif 4 spans the
second cytoplasmic loop; motif 5 lies in the third cytoplasmic loop; motifs
6 and 7 lie at the C-terminus. A single iteration on OWL29.1 was required
to reach convergence, no further sequences being identified beyond the
starting set. Several partial matches were found, all of which are either
fragments (HSOXYTOC and CJOXYR) or closely related mesotocin (BMMREC)
and isotocin receptors (I50130) that fail to make significant matches with
one or more motifs. 
 
An update on SPTR37_9f identified a true set of 7 sequences, and 2
partial matches.
Summary Information
   7 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
77777777
60000000
50000000
40011011
30000000
20001010
1234567
True Positives
OXYR_BOVIN    OXYR_HUMAN    OXYR_MACMU    OXYR_MOUSE    
OXYR_PIG OXYR_RAT OXYR_SHEEP
True Positive Partials
Codes involving 4 elements
Q90252
Codes involving 2 elements
Q90334
Sequence Titles
OXYR_BOVIN  Oxytocin receptor (OT-R) - Bos taurus (Bovine). 
OXYR_HUMAN Oxytocin receptor (OT-R) - Homo sapiens (Human).
OXYR_MACMU Oxytocin receptor (OT-R) - Macaca mulatta (Rhesus macaque).
OXYR_MOUSE Oxytocin receptor (OT-R) - Mus musculus (Mouse).
OXYR_PIG Oxytocin receptor (OT-R) - Sus scrofa (Pig).
OXYR_RAT Oxytocin receptor (OT-R) - Rattus norvegicus (Rat).
OXYR_SHEEP Oxytocin receptor (OT-R) - Ovis aries (Sheep).

Q90252 MESOTOCIN RECEPTOR (MTR) - BUFO MARINUS (GIANT TOAD) (CANE TOAD).

Q90334 ISOTOCIN RECEPTOR - CATOSTOMUS COMMERSONI (WHITE SUCKER).
Scan History
OWL29_1    1  100  NSINGLE    
SPTR37_9f 2 9 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
AEAVNGSAAPPGTE OSOXYTREC 11 11 -
VELDLGSGVPPGEE A55597 11 11 -
AEAANASAAPPGAE OXYR_HUMAN 11 11 -
AEAVNSSAAPPEAE OXYR_PIG 11 11 -

Motif 2 width=15
Element Seqn Id St Int Rpt
EGNRTAGPPQRNEAL A55597 24 -1 -
EGNRTAGPPRRNEAL OXYR_HUMAN 24 -1 -
EGNRTAGPPQRNEAL OXYR_PIG 24 -1 -
EGNRTAGPPQRNEAL OSOXYTREC 24 -1 -

Motif 3 width=15
Element Seqn Id St Int Rpt
RTTRHKHSRLFFFMK OSOXYTREC 65 26 -
RTTRHKHSRLFFFMK A55597 65 26 -
RTTRHKHSRLFFFMK OXYR_PIG 65 26 -
RTTRQKHSRLFFFMK OXYR_HUMAN 65 26 -

Motif 4 width=17
Element Seqn Id St Int Rpt
CLAICQPLRSLRRRTDR A55597 138 58 -
CLAICQPLRSLRRRTDR OXYR_HUMAN 138 58 -
CLAICQPLRALRRPADR OXYR_PIG 138 58 -
CLAICQPLRSLRRRTDR OSOXYTREC 138 58 -

Motif 5 width=14
Element Seqn Id St Int Rpt
NDAAGGAGRAALAR A55597 245 90 -
GAAADCAGRAALAR OSOXYTREC 248 93 -
GAAAGDGGRVALAR OXYR_HUMAN 246 91 -
GAAAGSRGRAALAR OXYR_PIG 248 93 -

Motif 6 width=17
Element Seqn Id St Int Rpt
FHELVQRFLCCSSSHLK OXYR_PIG 339 77 -
FHELVQRFFCCSARYLK A55597 336 77 -
FQDLVQRFLCCSFRRLK OSOXYTREC 339 77 -
FHELVQRFLCCSASYLK OXYR_HUMAN 337 77 -

Motif 7 width=20
Element Seqn Id St Int Rpt
KKSNSSTFVLSQHSSSQKSC OXYR_PIG 366 10 -
KKSNSSTFVLSRRSSSQRSC A55597 363 10 -
KKSNSSSFVLSHRSSSQRSC OXYR_HUMAN 364 10 -
KKIHSYTFVLSRHSSSQRSC OSOXYTREC 366 10 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
TEAVNSSAAPPGAE OXYR_MACMU 11 11 -
AEAANASAAPPGAE OXYR_HUMAN 11 11 -
AEAVNGSAAPPGTE OXYR_SHEEP 11 11 -
AEAVNGSAAPPGTE OXYR_BOVIN 11 11 -
IELDLGSGVPPGAE OXYR_MOUSE 11 11 -
VELDLGSGVPPGEE OXYR_RAT 11 11 -
AEAVNSSAAPPEAE OXYR_PIG 11 11 -

Motif 2 width=15
Element Seqn Id St Int Rpt
EGNCTAGPPRRNEAL OXYR_MACMU 24 -1 -
EGNRTAGPPRRNEAL OXYR_HUMAN 24 -1 -
EGNRTAGPPQRNEAL OXYR_SHEEP 24 -1 -
EGNRTAGPPQRNEAL OXYR_BOVIN 24 -1 -
EGNLTAGPPRRNEAL OXYR_MOUSE 24 -1 -
EGNRTAGPPQRNEAL OXYR_RAT 24 -1 -
EGNRTAGPPQRNEAL OXYR_PIG 24 -1 -

Motif 3 width=15
Element Seqn Id St Int Rpt
RTTRHKHSRLFFFMK OXYR_MACMU 65 26 -
RTTRQKHSRLFFFMK OXYR_HUMAN 65 26 -
RTTRHKHSRLFFFMK OXYR_SHEEP 65 26 -
RTTRHKHSRLFFFMK OXYR_BOVIN 65 26 -
RTTRHKHSRLFFFMK OXYR_MOUSE 65 26 -
RTTRHKHSRLFFFMK OXYR_RAT 65 26 -
RTTRHKHSRLFFFMK OXYR_PIG 65 26 -

Motif 4 width=17
Element Seqn Id St Int Rpt
CLAICQPLRSLRRRTDR OXYR_MACMU 138 58 -
CLAICQPLRSLRRRTDR OXYR_HUMAN 138 58 -
CLAICQPLRSLRRRTDR OXYR_SHEEP 138 58 -
CLAICQPLRSLSRRTDR OXYR_BOVIN 138 58 -
CLAICQPLRSLRRRTDR OXYR_MOUSE 138 58 -
CLAICQPLRSLRRRTDR OXYR_RAT 138 58 -
CLAICQPLRALRRPADR OXYR_PIG 138 58 -

Motif 5 width=14
Element Seqn Id St Int Rpt
GAAAGDGGRMALAR OXYR_MACMU 246 91 -
GAAAGDGGRVALAR OXYR_HUMAN 246 91 -
GAAADCAGRAALAR OXYR_SHEEP 248 93 -
GEAADWAGRAILAR OXYR_BOVIN 248 93 -
SDAAGGAGRAALAR OXYR_MOUSE 245 90 -
NDAAGGAGRAALAR OXYR_RAT 245 90 -
GAAAGSRGRAALAR OXYR_PIG 248 93 -

Motif 6 width=17
Element Seqn Id St Int Rpt
FHELVQRFLCCSASYLK OXYR_MACMU 337 77 -
FHELVQRFLCCSASYLK OXYR_HUMAN 337 77 -
FQDLVQRFLCCSFRRLK OXYR_SHEEP 339 77 -
FQELVQRFLCCSFRRLK OXYR_BOVIN 339 77 -
FHELVQRFLCCSARYLK OXYR_MOUSE 336 77 -
FHELVQRFFCCSARYLK OXYR_RAT 336 77 -
FHELVQRFLCCSSSHLK OXYR_PIG 339 77 -

Motif 7 width=20
Element Seqn Id St Int Rpt
KKSNSSSFVLSHRSSSQRSC OXYR_MACMU 364 10 -
KKSNSSSFVLSHRSSSQRSC OXYR_HUMAN 364 10 -
KKIHSYTFVLSRHSSSQRSC OXYR_SHEEP 366 10 -
KKSNSSTFVLSQYSSSQRRC OXYR_BOVIN 366 10 -
KKSNSSTFVLSRCSSSQRSC OXYR_MOUSE 363 10 -
KKSNSSTFVLSRRSSSQRSC OXYR_RAT 363 10 -
KKSNSSTFVLSQHSSSQKSC OXYR_PIG 366 10 -