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PR00664

Identifier
OCTOPAMINER  [View Relations]  [View Alignment]  
Accession
PR00664
No. of Motifs
7
Creation Date
22-FEB-1997  (UPDATE 03-JUL-1999)
Title
Octopamine receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN

INTERPRO; IPR002002
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. ARAKAWA, S., GOCAYNE, J.D., MCCOMBIE, W.R., URQUHART, D.A., 
HALL, L.M., FRASER, C.M. AND VENTER J.C.
Cloning, localization, and permanent expression of a Drosophila 
octopamine receptor.
NEURON 4(3) 343-354 (1990).
 
7. SAUDOU, F., AMLAIKY, N., PLASSAT, J.L., BORRELLI, E. AND HEN, R.
Cloning and characterization of a Drosophila tyramine receptor.
EMBO J. 9(11) 3611-3617 (1990).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The insect octopamine receptor mediates the attenuation of adenylate cyclase 
activity. Sequence and pharmacological comparisons indicate that the 
octopamine receptor is unique, but closely related to mammalian adrenergic 
receptors, perhaps as an evolutionary precursor [6,7]. 
 
OCTOPAMINER is a 7-element fingerprint that provides a signature for the
octopamine receptors. The fingerprint was derived from an initial alignment
of 5 sequences: the motifs were drawn from conserved sections within either
loop or TM regions, focusing on those areas of the alignment that
characterise the octopamine receptors but distinguish them from the rest
of the rhodopsin-like superfamily - motif 1 lies in the N-terminal region
leading into TM domain 1; motif 2 spans the first cytoplasmic loop; motif 3
lies in the first external loop; motif 4 lies in the second external loop; 
motifs 5 and 6 lie in the third cytoplasmic loop; and motif 7 lies in the
third external loop. A single iteration on OWL29.1 was required to reach
convergence, no further sequences being identified beyond the starting set. 
A single partial match was found, D83547, a Balanus amphitrite homologue.
 
An update on SPTR37_9f identified a true set of 5 sequences, and 4
partial matches.
Summary Information
   5 codes involving  7 elements
0 codes involving 6 elements
1 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
75555555
60000000
50111011
40111010
30000000
20020200
1234567
True Positives
OAR1_LOCMI    OAR2_LOCMI    OAR_BOMMO     OAR_DROME     
OAR_HELVI
True Positive Partials
Codes involving 5 elements
O77254
Codes involving 4 elements
Q93127
Codes involving 2 elements
O00537 O00590
Sequence Titles
OAR1_LOCMI  Tyramine receptor 1 (Tyr-Loc1) - Locusta migratoria (Migratory locust). 
OAR2_LOCMI Putative tyramine receptor 2 (Tyr-Loc2) - Locusta migratoria (Migratory locust).
OAR_BOMMO Octopamine receptor - Bombyx mori (Silk moth).
OAR_DROME Tyramine/octopamine receptor precursor (Tyr/Oct-Dro) - Drosophila melanogaster (Fruit fly).
OAR_HELVI Octopamine receptor - Heliothis virescens (Noctuid moth) (Owlet moth).

O77254 G-PROTEIN COUPLED RECEPTOR - BOOPHILUS MICROPLUS (CATTLE TICK).

Q93127 PUTATIVE TYRAMINE/OCTOPAMINE RECEPTOR - BALANUS AMPHITRITE.

O00537 CHEMOKINE RECEPTOR CCR-9 - HOMO SAPIENS (HUMAN).
O00590 CC-CHEMOKINE RECEPTOR 10 - HOMO SAPIENS (HUMAN).
Scan History
OWL29_1    1  50   NSINGLE    
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
GITLAVPEWEAICTA HVOCTOK50 42 42 -
GISLAVPEWEAICTA BMOCTOB96 44 44 -
GVRLAVPEWEVAVTA S58868 41 41 -
GVRLAVPEWEVAVTA S58869 41 41 -
GLQLAVPEWEALLTA OAR_DROME 99 99 -

Motif 2 width=18
Element Seqn Id St Int Rpt
VVLSVFTYKPLRIVQNFF S58869 72 16 -
VVLSVFTYKPLRIVQNFF S58868 72 16 -
VILSVFTYKPLRIVQNFF BMOCTOB96 75 16 -
VILSVFTYKPLRIVQNFF HVOCTOK50 73 16 -
VILSVFTYKPLRIVQNFF OAR_DROME 130 16 -

Motif 3 width=14
Element Seqn Id St Int Rpt
VFGIYVCKMWLTCD BMOCTOB96 121 28 -
EFGIHLCKLWLTCD OAR_DROME 176 28 -
VFGIVVCKMWLTCD S58868 118 28 -
VFGIVVCKMWLTCD S58869 118 28 -
VFGIYVCKMWLTCD HVOCTOK50 119 28 -

Motif 4 width=11
Element Seqn Id St Int Rpt
LLGWNDWPDVF HVOCTOK50 188 55 -
LLGWNDWPEVF BMOCTOB96 190 55 -
LIGWNDWPMEF S58869 187 55 -
LIGWNDWPMEF S58868 187 55 -
LIGWNDWPDEF OAR_DROME 245 55 -

Motif 5 width=13
Element Seqn Id St Int Rpt
LRERAKASKLNSA S58869 242 44 -
LRERAKASKLNSA S58868 242 44 -
LRDRAKATKISTI BMOCTOB96 245 44 -
LRDRAKATKISTI HVOCTOK50 243 44 -
LRERARANKLNTI OAR_DROME 300 44 -

Motif 6 width=14
Element Seqn Id St Int Rpt
VYQFIEEKQRISLS S58869 390 135 -
VYQFIEEKQRISLS S58868 390 135 -
VYQFIEEKQRISLT HVOCTOK50 384 128 -
VNQFIEEKQKISLS OAR_DROME 509 196 -
VYQFIEEKQRISLT BMOCTOB96 386 128 -

Motif 7 width=13
Element Seqn Id St Int Rpt
CKPSPKLVNFITW S58868 440 36 -
CCLSNKFINFITW BMOCTOB96 435 35 -
CCLSNKFINFITW HVOCTOK50 433 35 -
CCPTNKFKNFITW OAR_DROME 558 35 -
CKPSPKLVNFITW S58869 440 36 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
GVRLAVPEWEVAVTA OAR2_LOCMI 41 41 -
GVRLAVPEWEVAVTA OAR1_LOCMI 41 41 -
GITLAVPEWEAICTA OAR_HELVI 42 42 -
GISLAVPEWEAICTA OAR_BOMMO 44 44 -
GLQLAVPEWEALLTA OAR_DROME 99 99 -

Motif 2 width=18
Element Seqn Id St Int Rpt
VVLSVFTYKPLRIVQNFF OAR2_LOCMI 72 16 -
VVLSVFTYKPLRIVQNFF OAR1_LOCMI 72 16 -
VILSVFTYKPLRIVQNFF OAR_HELVI 73 16 -
VILSVFTYKPLRIVQNFF OAR_BOMMO 75 16 -
VILSVFTYKPLRIVQNFF OAR_DROME 130 16 -

Motif 3 width=14
Element Seqn Id St Int Rpt
VFGIVVCKMWLTCD OAR2_LOCMI 118 28 -
VFGIVVCKMWLTCD OAR1_LOCMI 118 28 -
VFGIYVCKMWLTCD OAR_HELVI 119 28 -
VFGIYVCKMWLTCD OAR_BOMMO 121 28 -
EFGIHLCKLWLTCD OAR_DROME 176 28 -

Motif 4 width=11
Element Seqn Id St Int Rpt
LIGWNDWPMEF OAR2_LOCMI 187 55 -
LIGWNDWPMEF OAR1_LOCMI 187 55 -
LLGWNDWPDVF OAR_HELVI 188 55 -
LLGWNDWPEVF OAR_BOMMO 190 55 -
LIGWNDWPDEF OAR_DROME 245 55 -

Motif 5 width=13
Element Seqn Id St Int Rpt
LRERAKASKLNSA OAR2_LOCMI 242 44 -
LRERAKASKLNSA OAR1_LOCMI 242 44 -
LRDRAKATKISTI OAR_HELVI 243 44 -
LRDRAKATKISTI OAR_BOMMO 245 44 -
LRERARANKLNTI OAR_DROME 300 44 -

Motif 6 width=14
Element Seqn Id St Int Rpt
VYQFIEEKQRISLS OAR2_LOCMI 390 135 -
VYQFIEEKQRISLS OAR1_LOCMI 390 135 -
VYQFIEEKQRISLT OAR_HELVI 384 128 -
VYQFIEEKQRISLT OAR_BOMMO 386 128 -
VNQFIEEKQKISLS OAR_DROME 509 196 -

Motif 7 width=13
Element Seqn Id St Int Rpt
CKPSPKLVNFITW OAR2_LOCMI 440 36 -
CKPSPKLVNFITW OAR1_LOCMI 440 36 -
CCLSNKFINFITW OAR_HELVI 433 35 -
CCLSNKFINFITW OAR_BOMMO 435 35 -
CCPTNKFKNFITW OAR_DROME 558 35 -