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PR00655

Identifier
AUXINBINDNGP  [View Relations]  [View Alignment]  
Accession
PR00655
No. of Motifs
7
Creation Date
09-APR-1997  (UPDATE 06-JUN-1999)
Title
Auxin binding protein signature
Database References

INTERPRO; IPR000526
Literature References
1. PALME, K., HESSE, T., CAMPOS, N., GARBERS, C., YANOFSKY, M.F.
AND SCHELL, J.
Molecular analysis of an auxin binding protein gene located on
chromosome 4 of Arabidopsis.
PLANT CELL 4(2) 193-201 (1992).
 
2. HESSE, T., FELDWISCH, J., BALSHUSEMANN, D., BAUW, G., PUYPE, M.,
VANDEKERCKHOVE, J., LOBLER, M., KLAMBT, D., SCHELL, J. AND PALME, K.
Molecular cloning and structural analysis of a gene from Zea mays
coding for a putative receptor for the plant hormone auxin.
EMBO J. 8(9) 2453-2461 (1989).

Documentation
Auxin binding protein is located in the lumen of the endoplasmic reticulum
(ER). The primary structure contains an N-terminal hydrophobic leader
sequence of 30-40 amino acids, which could represent a signal for
translocation of the protein to the ER [1,2]. The mature protein comprises
around 165 residues, and contains a number of potential N-glycosylation
sites. In vitro transport studies have demonstrated co-translational
glycosylation [1]. Retention within the lumen of the ER correlates with
an additional signal located at the C terminus, represented by the
sequence Lys-Asp-Glu-Leu, known to be responsible for preventing secretion
of proteins from the lumen of the ER in eucaryotic cells [1,2].
 
AUXINBINDNGP is a 7-element fingerprint that provides a signature for 
auxin binding proteins. The fingerprint was derived from an initial 
alignment of 9 sequences: the motifs were drawn from conserved regions
spanning the full length of the mature protein. A single iteration on
OWL29.2 was required to reach convergence, no further sequences being
identified beyond the starting set. A single partial match was found,
ABP5_MAIZE, a maize auxin binding protein fragment that lacks the 
portion of sequence bearing the final 2 motifs.
 
An update on SPTR37_9f identified a true set of 11 sequences.
Summary Information
11 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
711111111111111
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
ABP1_ARATH    ABP1_MAIZE    ABP1_TOBAC    ABP2_TOBAC    
ABP4_MAIZE O64412 O64413 P93271
Q39457 Q41193 Q43431
Sequence Titles
ABP1_ARATH  AUXIN-BINDING PROTEIN 1 PRECURSOR (ABP) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS). 
ABP1_MAIZE AUXIN-BINDING PROTEIN 1 PRECURSOR (ABP) (ERABP1) - ZEA MAYS (MAIZE).
ABP1_TOBAC AUXIN-BINDING PROTEIN T85 PRECURSOR (ABP) - NICOTIANA TABACUM (COMMON TOBACCO).
ABP2_TOBAC AUXIN-BINDING PROTEIN T92 PRECURSOR (ABP) - NICOTIANA TABACUM (COMMON TOBACCO).
ABP4_MAIZE AUXIN-BINDING PROTEIN 4 PRECURSOR (ABP) - ZEA MAYS (MAIZE).
O64412 AUXIN-BINDING PROTEIN - RAPHANUS SATIVUS (RADISH).
O64413 AUXIN-BINDING PROTEIN - AVENA SATIVA (OAT).
P93271 AUXIN BINDING PROTEIN - MALUS DOMESTICA (APPLE) (MALUS SYLVESTRIS).
Q39457 AUXIN BINDING PROTEIN 1 - CAPSICUM ANNUUM (BELL PEPPER).
Q41193 AUXIN-BINDING PROTEIN - ZEA MAYS (MAIZE).
Q43431 AUXIN-BINDING PROTEIN PRECURSOR - FRAGARIA ANANASSA (STRAWBERRY).
Scan History
OWL29_2    1  100  NSINGLE    
SPTR37_9f 2 12 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
SLVRDISRMQQRNYGREGFS ABP4_MAIZE 48 48 -
PIVRNISDLPQDNYGRPGLS ABP1_ARATH 42 42 -
SLVRDISQMPQSSYGIEGLS ABP1_MAIZE 45 45 -
PLVRNISELPQENYGRSGLS ABP2_TOBAC 28 28 -
PLVRNISELPQENYGRSGLS ABP1_TOBAC 28 28 -
PIVRNISELPQDNYGRGGLA MDU77952 33 33 -
PVVRNISELPQNSYGRGGLA FXAABP1GN 34 34 -
SLVRDISQMPQSSYGIEGLS MZEAUX 45 45 -
PLVRNISELPQNNYGRPGLS CANERABP1 25 25 -

Motif 2 width=21
Element Seqn Id St Int Rpt
HITVAGALNHGMKEVEVWLQT ABP1_MAIZE 65 0 -
HTTIAGSVLHGMKEIEVWLQT ABP2_TOBAC 48 0 -
HTTIAGSVLHGIQEIEVWLQT CANERABP1 45 0 -
HTTIAGSVLHGMKEIEVWLQT ABP1_TOBAC 48 0 -
HITVTGALAHGTKEVEVWLQT ABP4_MAIZE 68 0 -
HTTVAGSLLHGLKEVEVWLQT MDU77952 53 0 -
HTTVAGSLLHGLKEVEVWLQT FXAABP1GN 54 0 -
HITVAGALNHGMKEVEVWLQT MZEAUX 65 0 -
HMTVAGSVLHGMKEVEIWLQT ABP1_ARATH 62 0 -

Motif 3 width=25
Element Seqn Id St Int Rpt
PGSRTPIHRHSCEEIFVVLKGQGIL ABP1_TOBAC 71 2 -
PGSSTPIHRHSCEEVFVVLKGQGTL CANERABP1 68 2 -
PGSGTPIHRHSCEEVFVVLKGSGTL MDU77952 76 2 -
PGSGTPIHRHSCEEVFVVLKGSGTV FXAABP1GN 77 2 -
PGQRTPIHRHSCEEVFTVLKGKGTL MZEAUX 88 2 -
PGFRTPIHRHSCEEIFIVLKGQGTL ABP2_TOBAC 71 2 -
PGQRTPIHRHSCEEVFTVLKGKGTL ABP1_MAIZE 88 2 -
PGSETPIHRHSCEEVFVVLKGSGTL ABP1_ARATH 85 2 -
PGQRTPIHRHSCEEVFIVLKGKGTL ABP4_MAIZE 91 2 -

Motif 4 width=14
Element Seqn Id St Int Rpt
HSKYPGNPQEFHIF ABP2_TOBAC 102 6 -
HGNFPGKPIEFPIF ABP1_ARATH 115 5 -
SLKYPGQPQEVPVF ABP4_MAIZE 121 5 -
SLKYPGQPQEIPFF MZEAUX 118 5 -
HEKYPGKPQEFSIF FXAABP1GN 108 6 -
HGKFPGKPQEFSIF MDU77952 106 5 -
HSKYPGNPQEFHIF CANERABP1 99 6 -
HSKYPGNPQEFHIF ABP1_TOBAC 102 6 -
SLKYPGQPQEIPFF ABP1_MAIZE 118 5 -

Motif 5 width=16
Element Seqn Id St Int Rpt
FANSTFHIPVNDVHQV MDU77952 119 -1 -
FPNSTFHVPVNDAHQV CANERABP1 112 -1 -
FQNTTFSIPVSDPHQV MZEAUX 131 -1 -
FPNSTFHIPVNDVHQV ABP1_TOBAC 115 -1 -
FPNSTFHIPVNDVHQV ABP2_TOBAC 115 -1 -
FQNTTFSIPVNDPHQV ABP1_MAIZE 131 -1 -
FANSTIHIPINDAHQV ABP1_ARATH 128 -1 -
FQNTTFSIPVNDPHQV ABP4_MAIZE 134 -1 -
FANSTFQIPVNDVHQI FXAABP1GN 121 -1 -

Motif 6 width=23
Element Seqn Id St Int Rpt
HEDLQVLVVISRPPVKVFMYDDW ABP1_TOBAC 136 5 -
HEDLQVLVIISRPPAKIFLYDDW ABP1_MAIZE 152 5 -
HEDLQVLVIISRPPIKIFIYEDW ABP1_ARATH 148 4 -
HEDLQVLVIISRPPVKIFIYDDW ABP4_MAIZE 155 5 -
HEDLQVLVIISRPPAKIFLYDDW MZEAUX 152 5 -
HEDLQVLVVISRPPVKVFIYENW FXAABP1GN 142 5 -
HEDLQALVTVSRPPVKVFMYQDW MDU77952 140 5 -
HEDLQVLVVISRPPVKVFTYDDW CANERABP1 133 5 -
QEDLQVLDVISRPPVKVFMYDDW ABP2_TOBAC 136 5 -

Motif 7 width=20
Element Seqn Id St Int Rpt
WSMPHTASKLKFPYYWDEGC FXAABP1GN 164 -1 -
WSMPHTAAKLKFPYYWDEEC ABP2_TOBAC 158 -1 -
WSMPHTAAVLKFPFVWDEDC ABP1_MAIZE 174 -1 -
WFMPHTAARLKFPYYWDEQC ABP1_ARATH 170 -1 -
WSMPHTAAKLKFPYFWDEDC ABP4_MAIZE 177 -1 -
WSMPHTAAKLKFPYYWDEEC ABP1_TOBAC 158 -1 -
WSVPHTASKLKFPYYWDEEC CANERABP1 155 -1 -
WFMPHTAAKLRYPYYWDEEC MDU77952 162 -1 -
WSMPHTAAVLKFPFVWDEDC MZEAUX 174 -1 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
PLVRNISELPQENYGRSGLS ABP1_TOBAC 28 28 -
PLVRNISELPQENYGRSGLS ABP2_TOBAC 28 28 -
PLVRNISELPQNNYGRPGLS Q39457 25 25 -
SLVRDISQMPQSSYGIEGLS ABP1_MAIZE 45 45 -
SLVRDISQMPQSSYGIEGLS Q41193 45 45 -
PVVRNISELPQNSYGRGGLA Q43431 34 34 -
PIVRNISELPQDNYGRGGLA P93271 33 33 -
PIVRNISDLPQDNYGRPGLS ABP1_ARATH 42 42 -
SLVRDISRMQQRNYGREGFS ABP4_MAIZE 48 48 -
PIVRNISELPQDSYGIPGLT O64412 42 42 -
SVVKDINQMHQSNYGLEGLS O64413 50 50 -

Motif 2 width=21
Element Seqn Id St Int Rpt
HTTIAGSVLHGMKEIEVWLQT ABP1_TOBAC 48 0 -
HTTIAGSVLHGMKEIEVWLQT ABP2_TOBAC 48 0 -
HTTIAGSVLHGIQEIEVWLQT Q39457 45 0 -
HITVAGALNHGMKEVEVWLQT ABP1_MAIZE 65 0 -
HITVAGALNHGMKEVEVWLQT Q41193 65 0 -
HTTVAGSLLHGLKEVEVWLQT Q43431 54 0 -
HTTVAGSLLHGLKEVEVWLQT P93271 53 0 -
HMTVAGSVLHGMKEVEIWLQT ABP1_ARATH 62 0 -
HITVTGALAHGTKEVEVWLQT ABP4_MAIZE 68 0 -
HMTVAGSVLHGMKEVEIWLQT O64412 62 0 -
HITVGGALAHGMKEVEVLLET O64413 70 0 -

Motif 3 width=25
Element Seqn Id St Int Rpt
PGSRTPIHRHSCEEIFVVLKGQGIL ABP1_TOBAC 71 2 -
PGFRTPIHRHSCEEIFIVLKGQGTL ABP2_TOBAC 71 2 -
PGSSTPIHRHSCEEVFVVLKGQGTL Q39457 68 2 -
PGQRTPIHRHSCEEVFTVLKGKGTL ABP1_MAIZE 88 2 -
PGQRTPIHRHSCEEVFTVLKGKGTL Q41193 88 2 -
PGSGTPIHRHSCEEVFVVLKGSGTV Q43431 77 2 -
PGSGTPIHRHSCEEVFVVLKGSGTL P93271 76 2 -
PGSETPIHRHSCEEVFVVLKGSGTL ABP1_ARATH 85 2 -
PGQRTPIHRHSCEEVFIVLKGKGTL ABP4_MAIZE 91 2 -
PGAATPIHRHSCEEVFVVPKGSGTL O64412 85 2 -
AGQRTPIHRHSCEEVFVVLKGRGTL O64413 93 2 -

Motif 4 width=14
Element Seqn Id St Int Rpt
HSKYPGNPQEFHIF ABP1_TOBAC 102 6 -
HSKYPGNPQEFHIF ABP2_TOBAC 102 6 -
HSKYPGNPQEFHIF Q39457 99 6 -
SLKYPGQPQEIPFF ABP1_MAIZE 118 5 -
SLKYPGQPQEIPFF Q41193 118 5 -
HEKYPGKPQEFSIF Q43431 108 6 -
HGKFPGKPQEFSIF P93271 107 6 -
HGNFPGKPIEFPIF ABP1_ARATH 115 5 -
SLKYPGQPQEVPVF ABP4_MAIZE 121 5 -
HESVPGKPVEFPIS O64412 115 5 -
SLKYPGTPQEIPFS O64413 123 5 -

Motif 5 width=16
Element Seqn Id St Int Rpt
FPNSTFHIPVNDVHQV ABP1_TOBAC 115 -1 -
FPNSTFHIPVNDVHQV ABP2_TOBAC 115 -1 -
FPNSTFHVPVNDAHQV Q39457 112 -1 -
FQNTTFSIPVNDPHQV ABP1_MAIZE 131 -1 -
FQNTTFSIPVNDPHQV Q41193 131 -1 -
FANSTFQIPVNDVHQI Q43431 121 -1 -
FANSTFHIPVNDVHQV P93271 120 -1 -
FANSTIHIPINDAHQV ABP1_ARATH 128 -1 -
FQNTTFSIPVNDPHQV ABP4_MAIZE 134 -1 -
SANSTFHIPINDAHQV O64412 128 -1 -
SQNSTFTVPINDPHQV O64413 136 -1 -

Motif 6 width=23
Element Seqn Id St Int Rpt
HEDLQVLVVISRPPVKVFMYDDW ABP1_TOBAC 136 5 -
QEDLQVLDVISRPPVKVFMYDDW ABP2_TOBAC 136 5 -
HEDLQVLVVISRPPVKVFTYDDW Q39457 133 5 -
HEDLQVLVIISRPPAKIFLYDDW ABP1_MAIZE 152 5 -
HEDLQVLVIISRPPAKIFLYDDW Q41193 152 5 -
HEDLQVLVVISRPPVKVFIYENW Q43431 142 5 -
HEDLQALVTVSRPPVKVFMYQDW P93271 141 5 -
HEDLQVLVIISRPPIKIFIYEDW ABP1_ARATH 148 4 -
HEDLQVLVIISRPPVKIFIYDDW ABP4_MAIZE 155 5 -
HEDLQVLVIISRPPIKIFTYDDW O64412 148 4 -
HEDLQFLVIISRPPVKVFLYDDW O64413 157 5 -

Motif 7 width=20
Element Seqn Id St Int Rpt
WSMPHTAAKLKFPYYWDEEC ABP1_TOBAC 158 -1 -
WSMPHTAAKLKFPYYWDEEC ABP2_TOBAC 158 -1 -
WSVPHTASKLKFPYYWDEEC Q39457 155 -1 -
WSMPHTAAVLKFPFVWDEDC ABP1_MAIZE 174 -1 -
WSMPHTAAVLKFPFVWDEDC Q41193 174 -1 -
WSMPHTASKLKFPYYWDEGC Q43431 164 -1 -
WFMPHTAAKLRYPYYWDEEC P93271 163 -1 -
WFMPHTAARLKFPYYWDEQC ABP1_ARATH 170 -1 -
WSMPHTAAKLKFPYFWDEDC ABP4_MAIZE 177 -1 -
WFMPHTAARLKFPFYWDEQC O64412 170 -1 -
WSMPHTAAKLKFPFLWDEDC O64413 179 -1 -