| Literature References | 1. PALME, K., HESSE, T., CAMPOS, N., GARBERS, C., YANOFSKY, M.F.
AND SCHELL, J.
Molecular analysis of an auxin binding protein gene located on
chromosome 4 of Arabidopsis.
PLANT CELL 4(2) 193-201 (1992).
2. HESSE, T., FELDWISCH, J., BALSHUSEMANN, D., BAUW, G., PUYPE, M.,
VANDEKERCKHOVE, J., LOBLER, M., KLAMBT, D., SCHELL, J. AND PALME, K.
Molecular cloning and structural analysis of a gene from Zea mays
coding for a putative receptor for the plant hormone auxin.
EMBO J. 8(9) 2453-2461 (1989).
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| Documentation | Auxin binding protein is located in the lumen of the endoplasmic reticulum
(ER). The primary structure contains an N-terminal hydrophobic leader
sequence of 30-40 amino acids, which could represent a signal for
translocation of the protein to the ER [1,2]. The mature protein comprises
around 165 residues, and contains a number of potential N-glycosylation
sites. In vitro transport studies have demonstrated co-translational
glycosylation [1]. Retention within the lumen of the ER correlates with
an additional signal located at the C terminus, represented by the
sequence Lys-Asp-Glu-Leu, known to be responsible for preventing secretion
of proteins from the lumen of the ER in eucaryotic cells [1,2].
AUXINBINDNGP is a 7-element fingerprint that provides a signature for
auxin binding proteins. The fingerprint was derived from an initial
alignment of 9 sequences: the motifs were drawn from conserved regions
spanning the full length of the mature protein. A single iteration on
OWL29.2 was required to reach convergence, no further sequences being
identified beyond the starting set. A single partial match was found,
ABP5_MAIZE, a maize auxin binding protein fragment that lacks the
portion of sequence bearing the final 2 motifs.
An update on SPTR37_9f identified a true set of 11 sequences.
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